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Q8TAT6

- NPL4_HUMAN

UniProt

Q8TAT6 - NPL4_HUMAN

Protein

Nuclear protein localization protein 4 homolog

Gene

NPLOC4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope By similarity.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri580 – 60829RanBP2-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: HGNC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: HGNC
    2. Golgi organization Source: HGNC
    3. membrane fusion Source: HGNC

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00144.

    Protein family/group databases

    TCDBi3.A.16.1.1. the endoplasmic reticular retrotranslocon (er-rt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear protein localization protein 4 homolog
    Short name:
    Protein NPL4
    Gene namesi
    Name:NPLOC4
    Synonyms:KIAA1499, NPL4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:18261. NPLOC4.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Endoplasmic reticulum By similarity. Nucleus By similarity
    Note: Associated with the endoplasmic reticulum and nuclear.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. endoplasmic reticulum Source: HGNC
    3. nuclear outer membrane-endoplasmic reticulum membrane network Source: HGNC
    4. nucleus Source: UniProt

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA143485558.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 608607Nuclear protein localization protein 4 homologPRO_0000057941Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei179 – 1791N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8TAT6.
    PaxDbiQ8TAT6.
    PRIDEiQ8TAT6.

    PTM databases

    PhosphoSiteiQ8TAT6.

    Expressioni

    Tissue specificityi

    Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.1 Publication

    Gene expression databases

    ArrayExpressiQ8TAT6.
    BgeeiQ8TAT6.
    CleanExiHS_NPLOC4.
    GenevestigatoriQ8TAT6.

    Organism-specific databases

    HPAiHPA021560.
    HPA023295.

    Interactioni

    Subunit structurei

    Heterodimer with UFD1L. The heterodimer binds ubiquitinated proteins. The heterodimer binds to VCP and inhibits Golgi membrane fusion.1 Publication

    Protein-protein interaction databases

    BioGridi120798. 68 interactions.
    DIPiDIP-44059N.
    IntActiQ8TAT6. 16 interactions.
    MINTiMINT-3043958.
    STRINGi9606.ENSP00000331487.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TAT6.
    SMRiQ8TAT6. Positions 2-77, 579-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Binds ubiquitinated proteins via its RanBP2-type zinc finger.

    Sequence similaritiesi

    Belongs to the NPL4 family.Curated
    Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri580 – 60829RanBP2-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5100.
    HOGENOMiHOG000008139.
    HOVERGENiHBG052659.
    KOiK14015.
    OMAiECITAGM.
    OrthoDBiEOG71RXJG.
    PhylomeDBiQ8TAT6.
    TreeFamiTF314173.

    Family and domain databases

    InterProiIPR007717. NPL4.
    IPR024682. Npl4_Ub-like_dom.
    IPR007716. NPL4_Zn-bd_put.
    IPR016563. PolyUb_recognition_cplx_Npl4.
    IPR029071. Ubiquitin-rel_dom.
    IPR001876. Znf_RanBP2.
    [Graphical view]
    PfamiPF05021. NPL4. 1 hit.
    PF11543. UN_NPL4. 1 hit.
    PF05020. zf-NPL4. 1 hit.
    [Graphical view]
    PIRSFiPIRSF010052. Polyub_prc_Npl4. 1 hit.
    SMARTiSM00547. ZnF_RBZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
    PS50199. ZF_RANBP2_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8TAT6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR    50
    NKTGEITASS NKSLNLLKIK HGDLLFLFPS SLAGPSSEME TSVPPGFKVF 100
    GAPNVVEDEI DQYLSKQDGK IYRSRDPQLC RHGPLGKCVH CVPLEPFDED 150
    YLNHLEPPVK HMSFHAYIRK LTGGADKGKF VALENISCKI KSGCEGHLPW 200
    PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF WRKTGNQHFG 250
    YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA 300
    AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK 350
    HPNMCRLSPD GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL 400
    LPCKDAPELG YAKESSSEQY VPDVFYKDVD KFGNEITQLA RPLPVEYLII 450
    DITTTFPKDP VYTFSISQNP FPIENRDVLG ETQDFHSLAT YLSQNTSSVF 500
    LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA QTWKRSEQWA 550
    TIEQLCSTVG GQLPGLHEYG AVGGSTHTAT AAMWACQHCT FMNQPGTGHC 600
    EMCSLPRT 608
    Length:608
    Mass (Da):68,120
    Last modified:January 23, 2007 - v3
    Checksum:i6ED6A0145F15C01D
    GO
    Isoform 2 (identifier: Q8TAT6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         558-608: TVGGQLPGLH...HCEMCSLPRT → EYPHPLPRHP...PPCQARGRLR

    Note: May be due to an intron retention. No experimental confirmation available.

    Show »
    Length:617
    Mass (Da):69,461
    Checksum:iB295DB62353575A3
    GO

    Sequence cautioni

    The sequence BAA91314.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAA96023.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAB14372.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAB14499.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti246 – 2461N → S in BAB14499. (PubMed:14702039)Curated
    Sequence conflicti248 – 2481H → Q in BAA91314. (PubMed:14702039)Curated
    Sequence conflicti573 – 5731G → E in BAA91314. (PubMed:14702039)Curated
    Sequence conflicti592 – 5921M → V in BAB14499. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei558 – 60851TVGGQ…SLPRT → EYPHPLPRHPVAGAGEQPTL HSSPLPVVPWIPHPAASWQV PSAMQRVETRPPCQARGRLR in isoform 2. 1 PublicationVSP_009789Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB040932 mRNA. Translation: BAA96023.1. Different initiation.
    AK000664 mRNA. Translation: BAA91314.1. Different initiation.
    AK023046 mRNA. Translation: BAB14372.1. Different initiation.
    AK023272 mRNA. Translation: BAB14499.1. Different initiation.
    BC025930 mRNA. Translation: AAH25930.1.
    AL834300 mRNA. Translation: CAD38971.1.
    CCDSiCCDS45812.1. [Q8TAT6-1]
    RefSeqiNP_060391.2. NM_017921.2. [Q8TAT6-1]
    UniGeneiHs.464333.

    Genome annotation databases

    EnsembliENST00000331134; ENSP00000331487; ENSG00000182446. [Q8TAT6-1]
    ENST00000374747; ENSP00000363879; ENSG00000182446. [Q8TAT6-2]
    GeneIDi55666.
    KEGGihsa:55666.
    UCSCiuc002kat.4. human. [Q8TAT6-1]
    uc002kau.3. human. [Q8TAT6-2]

    Polymorphism databases

    DMDMi50428974.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB040932 mRNA. Translation: BAA96023.1 . Different initiation.
    AK000664 mRNA. Translation: BAA91314.1 . Different initiation.
    AK023046 mRNA. Translation: BAB14372.1 . Different initiation.
    AK023272 mRNA. Translation: BAB14499.1 . Different initiation.
    BC025930 mRNA. Translation: AAH25930.1 .
    AL834300 mRNA. Translation: CAD38971.1 .
    CCDSi CCDS45812.1. [Q8TAT6-1 ]
    RefSeqi NP_060391.2. NM_017921.2. [Q8TAT6-1 ]
    UniGenei Hs.464333.

    3D structure databases

    ProteinModelPortali Q8TAT6.
    SMRi Q8TAT6. Positions 2-77, 579-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120798. 68 interactions.
    DIPi DIP-44059N.
    IntActi Q8TAT6. 16 interactions.
    MINTi MINT-3043958.
    STRINGi 9606.ENSP00000331487.

    Protein family/group databases

    TCDBi 3.A.16.1.1. the endoplasmic reticular retrotranslocon (er-rt) family.

    PTM databases

    PhosphoSitei Q8TAT6.

    Polymorphism databases

    DMDMi 50428974.

    Proteomic databases

    MaxQBi Q8TAT6.
    PaxDbi Q8TAT6.
    PRIDEi Q8TAT6.

    Protocols and materials databases

    DNASUi 55666.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000331134 ; ENSP00000331487 ; ENSG00000182446 . [Q8TAT6-1 ]
    ENST00000374747 ; ENSP00000363879 ; ENSG00000182446 . [Q8TAT6-2 ]
    GeneIDi 55666.
    KEGGi hsa:55666.
    UCSCi uc002kat.4. human. [Q8TAT6-1 ]
    uc002kau.3. human. [Q8TAT6-2 ]

    Organism-specific databases

    CTDi 55666.
    GeneCardsi GC17M079523.
    HGNCi HGNC:18261. NPLOC4.
    HPAi HPA021560.
    HPA023295.
    MIMi 606590. gene.
    neXtProti NX_Q8TAT6.
    PharmGKBi PA143485558.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5100.
    HOGENOMi HOG000008139.
    HOVERGENi HBG052659.
    KOi K14015.
    OMAi ECITAGM.
    OrthoDBi EOG71RXJG.
    PhylomeDBi Q8TAT6.
    TreeFami TF314173.

    Enzyme and pathway databases

    UniPathwayi UPA00144 .

    Miscellaneous databases

    ChiTaRSi NPLOC4. human.
    GeneWikii NPLOC4.
    GenomeRNAii 55666.
    NextBioi 60422.
    PROi Q8TAT6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8TAT6.
    Bgeei Q8TAT6.
    CleanExi HS_NPLOC4.
    Genevestigatori Q8TAT6.

    Family and domain databases

    InterProi IPR007717. NPL4.
    IPR024682. Npl4_Ub-like_dom.
    IPR007716. NPL4_Zn-bd_put.
    IPR016563. PolyUb_recognition_cplx_Npl4.
    IPR029071. Ubiquitin-rel_dom.
    IPR001876. Znf_RanBP2.
    [Graphical view ]
    Pfami PF05021. NPL4. 1 hit.
    PF11543. UN_NPL4. 1 hit.
    PF05020. zf-NPL4. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF010052. Polyub_prc_Npl4. 1 hit.
    SMARTi SM00547. ZnF_RBZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS01358. ZF_RANBP2_1. 1 hit.
    PS50199. ZF_RANBP2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
      DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas.
    4. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
      Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
      Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-8.
      Tissue: Platelet.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-608.
      Tissue: Melanoma.
    6. "Cloning and characterization of the gene encoding human NPL4, a protein interacting with the ubiquitin fusion-degradation protein (UFD1L)."
      Botta A., Tandoi C., Fini G., Calabrese G., Dallapiccola B., Novelli G.
      Gene 275:39-46(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UFD1L, TISSUE SPECIFICITY.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNPL4_HUMAN
    AccessioniPrimary (citable) accession number: Q8TAT6
    Secondary accession number(s): Q8N3J1
    , Q9H8V2, Q9H964, Q9NWR5, Q9P229
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3