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Q8TAT6 (NPL4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear protein localization protein 4 homolog

Short name=Protein NPL4
Gene names
Name:NPLOC4
Synonyms:KIAA1499, NPL4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope By similarity.

Pathway

Protein degradation; proteasomal ubiquitin-dependent pathway.

Subunit structure

Heterodimer with UFD1L. The heterodimer binds ubiquitinated proteins. The heterodimer binds to VCP and inhibits Golgi membrane fusion.

Subcellular location

Cytoplasmcytosol By similarity. Endoplasmic reticulum By similarity. Nucleus By similarity. Note: Associated with the endoplasmic reticulum and nuclear By similarity.

Tissue specificity

Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver. Ref.6

Domain

Binds ubiquitinated proteins via its RanBP2-type zinc finger.

Sequence similarities

Belongs to the NPL4 family.

Contains 1 RanBP2-type zinc finger.

Sequence caution

The sequence BAA91314.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA96023.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB14372.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB14499.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TAT6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8TAT6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     558-608: TVGGQLPGLH...HCEMCSLPRT → EYPHPLPRHP...PPCQARGRLR
Note: May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4 Ref.7
Chain2 – 608607Nuclear protein localization protein 4 homolog
PRO_0000057941

Regions

Zinc finger580 – 60829RanBP2-type

Amino acid modifications

Modified residue21N-acetylalanine Ref.7
Modified residue1791N6-acetyllysine By similarity

Natural variations

Alternative sequence558 – 60851TVGGQ…SLPRT → EYPHPLPRHPVAGAGEQPTL HSSPLPVVPWIPHPAASWQV PSAMQRVETRPPCQARGRLR in isoform 2.
VSP_009789

Experimental info

Sequence conflict2461N → S in BAB14499. Ref.2
Sequence conflict2481H → Q in BAA91314. Ref.2
Sequence conflict5731G → E in BAA91314. Ref.2
Sequence conflict5921M → V in BAB14499. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 6ED6A0145F15C01D

FASTA60868,120
        10         20         30         40         50         60 
MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR NKTGEITASS 

        70         80         90        100        110        120 
NKSLNLLKIK HGDLLFLFPS SLAGPSSEME TSVPPGFKVF GAPNVVEDEI DQYLSKQDGK 

       130        140        150        160        170        180 
IYRSRDPQLC RHGPLGKCVH CVPLEPFDED YLNHLEPPVK HMSFHAYIRK LTGGADKGKF 

       190        200        210        220        230        240 
VALENISCKI KSGCEGHLPW PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF 

       250        260        270        280        290        300 
WRKTGNQHFG YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA 

       310        320        330        340        350        360 
AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK HPNMCRLSPD 

       370        380        390        400        410        420 
GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL LPCKDAPELG YAKESSSEQY 

       430        440        450        460        470        480 
VPDVFYKDVD KFGNEITQLA RPLPVEYLII DITTTFPKDP VYTFSISQNP FPIENRDVLG 

       490        500        510        520        530        540 
ETQDFHSLAT YLSQNTSSVF LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA 

       550        560        570        580        590        600 
QTWKRSEQWA TIEQLCSTVG GQLPGLHEYG AVGGSTHTAT AAMWACQHCT FMNQPGTGHC 


EMCSLPRT 

« Hide

Isoform 2 [UniParc].

Checksum: B295DB62353575A3
Show »

FASTA61769,461

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
[4]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-8.
Tissue: Platelet.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-608.
Tissue: Melanoma.
[6]"Cloning and characterization of the gene encoding human NPL4, a protein interacting with the ubiquitin fusion-degradation protein (UFD1L)."
Botta A., Tandoi C., Fini G., Calabrese G., Dallapiccola B., Novelli G.
Gene 275:39-46(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UFD1L, TISSUE SPECIFICITY.
[7]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB040932 mRNA. Translation: BAA96023.1. Different initiation.
AK000664 mRNA. Translation: BAA91314.1. Different initiation.
AK023046 mRNA. Translation: BAB14372.1. Different initiation.
AK023272 mRNA. Translation: BAB14499.1. Different initiation.
BC025930 mRNA. Translation: AAH25930.1.
AL834300 mRNA. Translation: CAD38971.1.
RefSeqNP_060391.2. NM_017921.2.
UniGeneHs.464333.

3D structure databases

ProteinModelPortalQ8TAT6.
SMRQ8TAT6. Positions 2-77, 579-608.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120798. 63 interactions.
DIPDIP-44059N.
IntActQ8TAT6. 16 interactions.
MINTMINT-3043958.
STRING9606.ENSP00000331487.

Protein family/group databases

TCDB3.A.16.1.1. the endoplasmic reticular retrotranslocon (er-rt) family.

PTM databases

PhosphoSiteQ8TAT6.

Polymorphism databases

DMDM50428974.

Proteomic databases

PaxDbQ8TAT6.
PRIDEQ8TAT6.

Protocols and materials databases

DNASU55666.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000331134; ENSP00000331487; ENSG00000182446. [Q8TAT6-1]
ENST00000374747; ENSP00000363879; ENSG00000182446. [Q8TAT6-2]
GeneID55666.
KEGGhsa:55666.
UCSCuc002kat.4. human. [Q8TAT6-1]
uc002kau.3. human. [Q8TAT6-2]

Organism-specific databases

CTD55666.
GeneCardsGC17M079523.
HGNCHGNC:18261. NPLOC4.
HPAHPA021560.
HPA023295.
MIM606590. gene.
neXtProtNX_Q8TAT6.
PharmGKBPA143485558.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5100.
HOGENOMHOG000008139.
HOVERGENHBG052659.
KOK14015.
OMAECITAGM.
OrthoDBEOG71RXJG.
PhylomeDBQ8TAT6.
TreeFamTF314173.

Enzyme and pathway databases

UniPathwayUPA00144.

Gene expression databases

ArrayExpressQ8TAT6.
BgeeQ8TAT6.
CleanExHS_NPLOC4.
GenevestigatorQ8TAT6.

Family and domain databases

InterProIPR007717. NPL4.
IPR024682. Npl4_Ub-like_dom.
IPR007716. NPL4_Zn-bd_put.
IPR016563. PolyUb_recognition_cplx_Npl4.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamPF05021. NPL4. 1 hit.
PF11543. UN_NPL4. 1 hit.
PF05020. zf-NPL4. 1 hit.
[Graphical view]
PIRSFPIRSF010052. Polyub_prc_Npl4. 1 hit.
SMARTSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
PROSITEPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNPLOC4. human.
GeneWikiNPLOC4.
GenomeRNAi55666.
NextBio60422.
PROQ8TAT6.
SOURCESearch...

Entry information

Entry nameNPL4_HUMAN
AccessionPrimary (citable) accession number: Q8TAT6
Secondary accession number(s): Q8N3J1 expand/collapse secondary AC list , Q9H8V2, Q9H964, Q9NWR5, Q9P229
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM