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Q8TAT6

- NPL4_HUMAN

UniProt

Q8TAT6 - NPL4_HUMAN

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Protein

Nuclear protein localization protein 4 homolog

Gene

NPLOC4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity).By similarity

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri580 – 60829RanBP2-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. zinc ion binding Source: InterPro

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: HGNC
  2. Golgi organization Source: HGNC
  3. membrane fusion Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00144.

Protein family/group databases

TCDBi3.A.16.1.1. the endoplasmic reticular retrotranslocon (er-rt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear protein localization protein 4 homolog
Short name:
Protein NPL4
Gene namesi
Name:NPLOC4
Synonyms:KIAA1499, NPL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:18261. NPLOC4.

Subcellular locationi

Cytoplasmcytosol By similarity. Endoplasmic reticulum By similarity. Nucleus By similarity
Note: Associated with the endoplasmic reticulum and nuclear.By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: HGNC
  2. nuclear outer membrane-endoplasmic reticulum membrane network Source: HGNC
  3. nucleus Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485558.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 608607Nuclear protein localization protein 4 homologPRO_0000057941Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei179 – 1791N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8TAT6.
PaxDbiQ8TAT6.
PRIDEiQ8TAT6.

PTM databases

PhosphoSiteiQ8TAT6.

Expressioni

Tissue specificityi

Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.1 Publication

Gene expression databases

BgeeiQ8TAT6.
CleanExiHS_NPLOC4.
ExpressionAtlasiQ8TAT6. baseline and differential.
GenevestigatoriQ8TAT6.

Organism-specific databases

HPAiHPA021560.
HPA023295.

Interactioni

Subunit structurei

Heterodimer with UFD1L. The heterodimer binds ubiquitinated proteins. The heterodimer binds to VCP and inhibits Golgi membrane fusion.1 Publication

Protein-protein interaction databases

BioGridi120798. 73 interactions.
DIPiDIP-44059N.
IntActiQ8TAT6. 16 interactions.
MINTiMINT-3043958.
STRINGi9606.ENSP00000331487.

Structurei

3D structure databases

ProteinModelPortaliQ8TAT6.
SMRiQ8TAT6. Positions 2-77, 579-608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Binds ubiquitinated proteins via its RanBP2-type zinc finger.

Sequence similaritiesi

Belongs to the NPL4 family.Curated
Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri580 – 60829RanBP2-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5100.
GeneTreeiENSGT00390000018731.
HOGENOMiHOG000008139.
HOVERGENiHBG052659.
InParanoidiQ8TAT6.
KOiK14015.
OMAiECITAGM.
OrthoDBiEOG71RXJG.
PhylomeDBiQ8TAT6.
TreeFamiTF314173.

Family and domain databases

InterProiIPR007717. NPL4.
IPR024682. Npl4_Ub-like_dom.
IPR007716. NPL4_Zn-bd_put.
IPR016563. PolyUb_recognition_cplx_Npl4.
IPR029071. Ubiquitin-rel_dom.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF05021. NPL4. 1 hit.
PF11543. UN_NPL4. 1 hit.
PF05020. zf-NPL4. 1 hit.
[Graphical view]
PIRSFiPIRSF010052. Polyub_prc_Npl4. 1 hit.
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8TAT6) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR
60 70 80 90 100
NKTGEITASS NKSLNLLKIK HGDLLFLFPS SLAGPSSEME TSVPPGFKVF
110 120 130 140 150
GAPNVVEDEI DQYLSKQDGK IYRSRDPQLC RHGPLGKCVH CVPLEPFDED
160 170 180 190 200
YLNHLEPPVK HMSFHAYIRK LTGGADKGKF VALENISCKI KSGCEGHLPW
210 220 230 240 250
PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF WRKTGNQHFG
260 270 280 290 300
YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA
310 320 330 340 350
AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK
360 370 380 390 400
HPNMCRLSPD GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL
410 420 430 440 450
LPCKDAPELG YAKESSSEQY VPDVFYKDVD KFGNEITQLA RPLPVEYLII
460 470 480 490 500
DITTTFPKDP VYTFSISQNP FPIENRDVLG ETQDFHSLAT YLSQNTSSVF
510 520 530 540 550
LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA QTWKRSEQWA
560 570 580 590 600
TIEQLCSTVG GQLPGLHEYG AVGGSTHTAT AAMWACQHCT FMNQPGTGHC

EMCSLPRT
Length:608
Mass (Da):68,120
Last modified:January 23, 2007 - v3
Checksum:i6ED6A0145F15C01D
GO
Isoform 2 (identifier: Q8TAT6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     558-608: TVGGQLPGLH...HCEMCSLPRT → EYPHPLPRHP...PPCQARGRLR

Note: May be due to an intron retention. No experimental confirmation available.

Show »
Length:617
Mass (Da):69,461
Checksum:iB295DB62353575A3
GO

Sequence cautioni

The sequence BAA91314.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAA96023.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB14372.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB14499.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti246 – 2461N → S in BAB14499. (PubMed:14702039)Curated
Sequence conflicti248 – 2481H → Q in BAA91314. (PubMed:14702039)Curated
Sequence conflicti573 – 5731G → E in BAA91314. (PubMed:14702039)Curated
Sequence conflicti592 – 5921M → V in BAB14499. (PubMed:14702039)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei558 – 60851TVGGQ…SLPRT → EYPHPLPRHPVAGAGEQPTL HSSPLPVVPWIPHPAASWQV PSAMQRVETRPPCQARGRLR in isoform 2. 1 PublicationVSP_009789Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB040932 mRNA. Translation: BAA96023.1. Different initiation.
AK000664 mRNA. Translation: BAA91314.1. Different initiation.
AK023046 mRNA. Translation: BAB14372.1. Different initiation.
AK023272 mRNA. Translation: BAB14499.1. Different initiation.
BC025930 mRNA. Translation: AAH25930.1.
AL834300 mRNA. Translation: CAD38971.1.
CCDSiCCDS45812.1. [Q8TAT6-1]
RefSeqiNP_060391.2. NM_017921.2. [Q8TAT6-1]
UniGeneiHs.464333.

Genome annotation databases

EnsembliENST00000331134; ENSP00000331487; ENSG00000182446. [Q8TAT6-1]
ENST00000374747; ENSP00000363879; ENSG00000182446. [Q8TAT6-2]
GeneIDi55666.
KEGGihsa:55666.
UCSCiuc002kat.4. human. [Q8TAT6-1]
uc002kau.3. human. [Q8TAT6-2]

Polymorphism databases

DMDMi50428974.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB040932 mRNA. Translation: BAA96023.1 . Different initiation.
AK000664 mRNA. Translation: BAA91314.1 . Different initiation.
AK023046 mRNA. Translation: BAB14372.1 . Different initiation.
AK023272 mRNA. Translation: BAB14499.1 . Different initiation.
BC025930 mRNA. Translation: AAH25930.1 .
AL834300 mRNA. Translation: CAD38971.1 .
CCDSi CCDS45812.1. [Q8TAT6-1 ]
RefSeqi NP_060391.2. NM_017921.2. [Q8TAT6-1 ]
UniGenei Hs.464333.

3D structure databases

ProteinModelPortali Q8TAT6.
SMRi Q8TAT6. Positions 2-77, 579-608.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120798. 73 interactions.
DIPi DIP-44059N.
IntActi Q8TAT6. 16 interactions.
MINTi MINT-3043958.
STRINGi 9606.ENSP00000331487.

Protein family/group databases

TCDBi 3.A.16.1.1. the endoplasmic reticular retrotranslocon (er-rt) family.

PTM databases

PhosphoSitei Q8TAT6.

Polymorphism databases

DMDMi 50428974.

Proteomic databases

MaxQBi Q8TAT6.
PaxDbi Q8TAT6.
PRIDEi Q8TAT6.

Protocols and materials databases

DNASUi 55666.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000331134 ; ENSP00000331487 ; ENSG00000182446 . [Q8TAT6-1 ]
ENST00000374747 ; ENSP00000363879 ; ENSG00000182446 . [Q8TAT6-2 ]
GeneIDi 55666.
KEGGi hsa:55666.
UCSCi uc002kat.4. human. [Q8TAT6-1 ]
uc002kau.3. human. [Q8TAT6-2 ]

Organism-specific databases

CTDi 55666.
GeneCardsi GC17M079523.
HGNCi HGNC:18261. NPLOC4.
HPAi HPA021560.
HPA023295.
MIMi 606590. gene.
neXtProti NX_Q8TAT6.
PharmGKBi PA143485558.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5100.
GeneTreei ENSGT00390000018731.
HOGENOMi HOG000008139.
HOVERGENi HBG052659.
InParanoidi Q8TAT6.
KOi K14015.
OMAi ECITAGM.
OrthoDBi EOG71RXJG.
PhylomeDBi Q8TAT6.
TreeFami TF314173.

Enzyme and pathway databases

UniPathwayi UPA00144 .

Miscellaneous databases

ChiTaRSi NPLOC4. human.
GeneWikii NPLOC4.
GenomeRNAii 55666.
NextBioi 60422.
PROi Q8TAT6.
SOURCEi Search...

Gene expression databases

Bgeei Q8TAT6.
CleanExi HS_NPLOC4.
ExpressionAtlasi Q8TAT6. baseline and differential.
Genevestigatori Q8TAT6.

Family and domain databases

InterProi IPR007717. NPL4.
IPR024682. Npl4_Ub-like_dom.
IPR007716. NPL4_Zn-bd_put.
IPR016563. PolyUb_recognition_cplx_Npl4.
IPR029071. Ubiquitin-rel_dom.
IPR001876. Znf_RanBP2.
[Graphical view ]
Pfami PF05021. NPL4. 1 hit.
PF11543. UN_NPL4. 1 hit.
PF05020. zf-NPL4. 1 hit.
[Graphical view ]
PIRSFi PIRSF010052. Polyub_prc_Npl4. 1 hit.
SMARTi SM00547. ZnF_RBZ. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  4. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-8.
    Tissue: Platelet.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-608.
    Tissue: Melanoma.
  6. "Cloning and characterization of the gene encoding human NPL4, a protein interacting with the ubiquitin fusion-degradation protein (UFD1L)."
    Botta A., Tandoi C., Fini G., Calabrese G., Dallapiccola B., Novelli G.
    Gene 275:39-46(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UFD1L, TISSUE SPECIFICITY.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNPL4_HUMAN
AccessioniPrimary (citable) accession number: Q8TAT6
Secondary accession number(s): Q8N3J1
, Q9H8V2, Q9H964, Q9NWR5, Q9P229
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3