Q8TAS1 (UHMK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase Kist
EC=2.7.11.1

Alternative name(s):
Kinase interacting with stathmin
PAM COOH-terminal interactor protein 2
Short name=P-CIP2
U2AF homology motif kinase 1

Gene names

Name:	UHMK1
Synonyms:	KIS, KIST

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with stathmin and CDKN1B/p27Kip1 By similarity. Interacts with PAM. Ref.6 Ref.7
Subcellular location	Nucleus. Note: Mostly nuclear By similarity.
Tissue specificity	Widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes. Ref.7
Induction	By serum growth factors By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle arrest Inferred from mutant phenotype Ref.7. Source: HGNC neuron projection development Inferred from sequence or structural similarity. Source: BHF-UCL peptidyl-serine phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of translational initiation Inferred from sequence or structural similarity. Source: BHF-UCL protein autophosphorylation Inferred from direct assay Ref.7. Source: HGNC regulation of protein export from nucleus Inferred from direct assay Ref.7. Source: HGNC
Cellular_component	axon Inferred from sequence or structural similarity. Source: BHF-UCL dendrite cytoplasm Inferred from sequence or structural similarity. Source: BHF-UCL neuronal ribonucleoprotein granule Inferred from sequence or structural similarity. Source: BHF-UCL nucleus Inferred from direct assay Ref.7. Source: HGNC
Molecular_function	ATP binding Inferred by curator Ref.7. Source: HGNC RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from direct assay Ref.7. Source: HGNC ribonucleoprotein complex binding Inferred from sequence or structural similarity. Source: BHF-UCL
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TAS1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TAS1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     342-344: DVV → GLC
     345-419: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8TAS1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: MAGSGCAWGA...LEQLQGHRNI → MFLTRPKVCVDLNRR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Serine/threonine-protein kinase Kist		PRO_0000086777
Regions
	Domain	23 – 304	282	Protein kinase
	Domain	324 – 406	83	RRM
	Nucleotide binding	29 – 37	9	ATP By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	54	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 89	89	MAGSG…GHRNI → MFLTRPKVCVDLNRR in isoform 3.		VSP_046145
	Alternative sequence	342 – 344	3	DVV → GLC in isoform 2.		VSP_004908
	Alternative sequence	345 – 419	75	Missing in isoform 2.		VSP_004909
	Natural variant	159	1	L → V. Ref.8 Corresponds to variant rs34466082 [ dbSNP | Ensembl ].		VAR_041272
	Natural variant	197	1	Y → D. Ref.8 Corresponds to variant rs56201055 [ dbSNP | Ensembl ].		VAR_041273
Experimental info
	Mutagenesis	54	1	K → A: Loss of kinase activity. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 19, 2002. Version 2. Checksum: 903E982EE12A8CF8	FASTA	419	46,546
	10 20 30 40 50 60 MAGSGCAWGA EPPRFLEAFG RLWQVQSRLG SGSSASVYRV RCCGNPGSPP GALKQFLPPG 70 80 90 100 110 120 TTGAAASAAE YGFRKERAAL EQLQGHRNIV TLYGVFTIHF SPNVPSRCLL LELLDVSVSE 130 140 150 160 170 180 LLLYSSHQGC SMWMIQHCAR DVLEALAFLH HEGYVHADLK PRNILWSAEN ECFKLIDFGL 190 200 210 220 230 240 SFKEGNQDVK YIQTDGYRAP EAELQNCLAQ AGLQSDTECT SAVDLWSLGI ILLEMFSGMK 250 260 270 280 290 300 LKHTVRSQEW KANSSAIIDH IFASKAVVNA AIPAYHLRDL IKSMLHDDPS RRIPAEMALC 310 320 330 340 350 360 SPFFSIPFAP HIEDLVMLPT PVLRLLNVLD DDYLENEEEY EDVVEDVKEE CQKYGPVVSL 370 380 390 400 410 LVPKENPGRG QVFVEYANAG DSKAAQKLLT GRMFDGKFVV ATFYPLSAYK RGYLYQTLL
	Isoform 2 [UniParc]. Checksum: F17050CA387CCA9B	FASTA	344	38,130
	10 20 30 40 50 60 MAGSGCAWGA EPPRFLEAFG RLWQVQSRLG SGSSASVYRV RCCGNPGSPP GALKQFLPPG 70 80 90 100 110 120 TTGAAASAAE YGFRKERAAL EQLQGHRNIV TLYGVFTIHF SPNVPSRCLL LELLDVSVSE 130 140 150 160 170 180 LLLYSSHQGC SMWMIQHCAR DVLEALAFLH HEGYVHADLK PRNILWSAEN ECFKLIDFGL 190 200 210 220 230 240 SFKEGNQDVK YIQTDGYRAP EAELQNCLAQ AGLQSDTECT SAVDLWSLGI ILLEMFSGMK 250 260 270 280 290 300 LKHTVRSQEW KANSSAIIDH IFASKAVVNA AIPAYHLRDL IKSMLHDDPS RRIPAEMALC 310 320 330 340 SPFFSIPFAP HIEDLVMLPT PVLRLLNVLD DDYLENEEEY EGLC
	Isoform 3 [UniParc]. Checksum: 2D542DC30505CF01	FASTA	345	39,044
	10 20 30 40 50 60 MFLTRPKVCV DLNRRVTLYG VFTIHFSPNV PSRCLLLELL DVSVSELLLY SSHQGCSMWM 70 80 90 100 110 120 IQHCARDVLE ALAFLHHEGY VHADLKPRNI LWSAENECFK LIDFGLSFKE GNQDVKYIQT 130 140 150 160 170 180 DGYRAPEAEL QNCLAQAGLQ SDTECTSAVD LWSLGIILLE MFSGMKLKHT VRSQEWKANS 190 200 210 220 230 240 SAIIDHIFAS KAVVNAAIPA YHLRDLIKSM LHDDPSRRIP AEMALCSPFF SIPFAPHIED 250 260 270 280 290 300 LVMLPTPVLR LLNVLDDDYL ENEEEYEDVV EDVKEECQKY GPVVSLLVPK ENPGRGQVFV 310 320 330 340 EYANAGDSKA AQKLLTGRMF DGKFVVATFY PLSAYKRGYL YQTLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Quantitative RT-PCR reveals a ubiquitous but preferentially neural expression of the KIS gene in rat and human." Bieche I., Manceau V., Curmi P.A., Laurendeau I., Lachkar S., Leroy K., Vidaud D., Sobel A., Maucuer A. Brain Res. Mol. Brain Res. 114:55-64(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Testis.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye and Skin.
[6]	"The novel kinase peptidylglycine alpha-amidating monooxygenase cytosolic interactor protein 2 interacts with the cytosolic routing determinants of the peptide processing enzyme peptidylglycine alpha-amidating monooxygenase." Caldwell B.D., Darlington D.N., Penzes P., Johnson R.C., Eipper B.A., Mains R.E. J. Biol. Chem. 274:34646-34656(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PAM.
[7]	"A growth factor-dependent nuclear kinase phosphorylates p27(Kip1) and regulates cell cycle progression." Boehm M., Yoshimoto T., Crook M.F., Nallamshetty S., True A., Nabel G.J., Nabel E.G. EMBO J. 21:3390-3401(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CDKN1B, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-54.
[8]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] VAL-159 AND ASP-197.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ536197 mRNA. Translation: CAD60192.1. AK058195 mRNA. No translation available. AK292369 mRNA. Translation: BAF85058.1. AL359699 Genomic DNA. Translation: CAH70392.1. CH471067 Genomic DNA. Translation: EAW90706.1. CH471067 Genomic DNA. Translation: EAW90707.1. BC014917 mRNA. Translation: AAH14917.1. BC026046 mRNA. Translation: AAH26046.1.
IPI	IPI00163376. IPI00220575.
RefSeq	NP_001171692.1. NM_001184763.1. NP_653225.2. NM_144624.2. NP_787062.1. NM_175866.4.
UniGene	Hs.127310.
3D structure databases
ProteinModelPortal	Q8TAS1.
SMR	Q8TAS1. Positions 22-304, 318-415.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TAS1. 1 interaction.
MINT	MINT-7241640.
STRING	9606.ENSP00000420270.
PTM databases
PhosphoSite	Q8TAS1.
Polymorphism databases
DMDM	24211880.
Proteomic databases
PaxDb	Q8TAS1.
PRIDE	Q8TAS1.
Protocols and materials databases
DNASU	127933.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000489294; ENSP00000420270; ENSG00000152332. ENST00000538489; ENSP00000446416; ENSG00000152332. ENST00000545294; ENSP00000441226; ENSG00000152332.
GeneID	127933.
KEGG	hsa:127933.
UCSC	uc001gcc.2. human. uc009wuu.2. human.
Organism-specific databases
CTD	127933.
GeneCards	GC01P162467.
HGNC	HGNC:19683. UHMK1.
MIM	608849. gene.
neXtProt	NX_Q8TAS1.
PharmGKB	PA134974001.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000013166.
HOVERGEN	HBG056149.
InParanoid	Q8TAS1.
KO	K08877.
OMA	YGVFTNH.
OrthoDB	EOG4X6C8F.
PhylomeDB	Q8TAS1.
Gene expression databases
ArrayExpress	Q8TAS1.
Bgee	Q8TAS1.
CleanEx	HS_UHMK1.
Genevestigator	Q8TAS1.
GermOnline	ENSG00000152332. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR012677. Nucleotide-bd_a/b_plait. IPR000719. Prot_kinase_cat_dom. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	UHMK1. human.
GenomeRNAi	127933.
NextBio	82204.
SOURCE	Search...

Entry information

Entry name

UHMK1_HUMAN

Accession

Primary (citable) accession number: Q8TAS1
Secondary accession number(s): A8K8K4, G3V1M1, Q96C22

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.