Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

M-phase-specific PLK1-interacting protein

Gene

MPLKIP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in maintenance of cell cycle integrity by regulating mitosis or cytokinesis.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase-specific PLK1-interacting protein
Alternative name(s):
TTD non-photosensitive 1 protein
Gene namesi
Name:MPLKIP
Synonyms:C7orf11, TTDN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:16002. MPLKIP.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • Golgi apparatus Source: HPA
  • midbody Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Involvement in diseasei

Trichothiodystrophy non-photosensitive 1 (TTDN1)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionTTDN1 is an autosomal recessive disease characterized by sulfur-deficient brittle hair, intellectual impairment, decreased male fertility and no cutaneous photosensitivity.

See also OMIM:234050
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti144 – 1441M → V in TTDN1. 1 Publication
VAR_022940

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi93 – 931S → A: Partially prevents phosphorylation. 1 Publication
Mutagenesisi104 – 1041S → A: Has no effect on interaction with PLK1 in mitosis. Partially prevents phosphorylation. 1 Publication
Mutagenesisi120 – 1201T → A: Abolishes interaction with PLK1 in mitosis. 1 Publication

Keywords - Diseasei

Disease mutation

Organism-specific databases

MIMi234050. phenotype.
Orphaneti33364. Trichothiodystrophy.
PharmGKBiPA25943.

Polymorphism and mutation databases

BioMutaiMPLKIP.
DMDMi71153365.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179M-phase-specific PLK1-interacting proteinPRO_0000065674Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei47 – 471Phosphoserine1 Publication
Modified residuei51 – 511Phosphothreonine1 Publication
Modified residuei72 – 721Phosphoserine2 Publications
Modified residuei80 – 801Phosphoserine2 Publications
Modified residuei82 – 821Phosphoserine1 Publication
Modified residuei93 – 931Phosphoserine2 Publications
Modified residuei104 – 1041Phosphoserine2 Publications
Modified residuei120 – 1201Phosphothreonine1 Publication
Modified residuei124 – 1241Phosphoserine1 Publication
Modified residuei133 – 1331Phosphoserine3 Publications

Post-translational modificationi

Phosphorylated during mitosis in the cell cycle probably by CDK1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8TAP9.
PaxDbiQ8TAP9.
PRIDEiQ8TAP9.

PTM databases

PhosphoSiteiQ8TAP9.

Expressioni

Tissue specificityi

Expressed at highest levels in liver and kidney; intermediate expression in skeletal muscle, pancreas, heart and placenta; low expression in brain and lung. Expressed in epidermis and hair follicles.2 Publications

Gene expression databases

BgeeiQ8TAP9.
CleanExiHS_C7orf11.
ExpressionAtlasiQ8TAP9. baseline and differential.
GenevisibleiQ8TAP9. HS.

Organism-specific databases

HPAiHPA058457.

Interactioni

Subunit structurei

Interacts with PLK1; phosphorylation-dependent.1 Publication

Protein-protein interaction databases

BioGridi126463. 14 interactions.
STRINGi9606.ENSP00000304553.

Structurei

3D structure databases

ProteinModelPortaliQ8TAP9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG45065.
GeneTreeiENSGT00390000002582.
HOGENOMiHOG000252982.
HOVERGENiHBG073319.
InParanoidiQ8TAP9.
OMAiSYSKSPA.
OrthoDBiEOG7BP84Z.
PhylomeDBiQ8TAP9.
TreeFamiTF335586.

Family and domain databases

InterProiIPR028265. MPLKIP-like.
IPR026618. MPLKIP_like_vertebrate.
[Graphical view]
PANTHERiPTHR22446. PTHR22446. 1 hit.
PfamiPF15502. MPLKIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TAP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRQNFRPPT PPYPGPGGGG WGSGSSFRGT PGGGGPRPPS PRDGYGSPHH
60 70 80 90 100
TPPYGPRSRP YGSSHSPRHG GSFPGGRFGS PSPGGYPGSY SRSPAGSQQQ
110 120 130 140 150
FGYSPGQQQT HPQGSPRTST PFGSGRVREK RMSNELENYF KPSMLEDPWA
160 170
GLEPVSVVDI SQQYSNTQTF TGKKGRYFC
Length:179
Mass (Da):19,147
Last modified:June 1, 2002 - v1
Checksum:i77DBA4FBDF9C70CC
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291G → E in a breast cancer sample; somatic mutation. 1 Publication
VAR_036273
Natural varianti144 – 1441M → V in TTDN1. 1 Publication
VAR_022940

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026265 mRNA. Translation: AAH26265.1.
CCDSiCCDS5463.1.
RefSeqiNP_619646.1. NM_138701.3.
UniGeneiHs.654989.

Genome annotation databases

EnsembliENST00000306984; ENSP00000304553; ENSG00000168303.
GeneIDi136647.
KEGGihsa:136647.
UCSCiuc003thl.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026265 mRNA. Translation: AAH26265.1.
CCDSiCCDS5463.1.
RefSeqiNP_619646.1. NM_138701.3.
UniGeneiHs.654989.

3D structure databases

ProteinModelPortaliQ8TAP9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126463. 14 interactions.
STRINGi9606.ENSP00000304553.

PTM databases

PhosphoSiteiQ8TAP9.

Polymorphism and mutation databases

BioMutaiMPLKIP.
DMDMi71153365.

Proteomic databases

MaxQBiQ8TAP9.
PaxDbiQ8TAP9.
PRIDEiQ8TAP9.

Protocols and materials databases

DNASUi136647.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306984; ENSP00000304553; ENSG00000168303.
GeneIDi136647.
KEGGihsa:136647.
UCSCiuc003thl.4. human.

Organism-specific databases

CTDi136647.
GeneCardsiGC07M040172.
HGNCiHGNC:16002. MPLKIP.
HPAiHPA058457.
MIMi234050. phenotype.
609188. gene.
neXtProtiNX_Q8TAP9.
Orphaneti33364. Trichothiodystrophy.
PharmGKBiPA25943.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG45065.
GeneTreeiENSGT00390000002582.
HOGENOMiHOG000252982.
HOVERGENiHBG073319.
InParanoidiQ8TAP9.
OMAiSYSKSPA.
OrthoDBiEOG7BP84Z.
PhylomeDBiQ8TAP9.
TreeFamiTF335586.

Miscellaneous databases

GeneWikiiC7orf11.
MPLKIP.
GenomeRNAii136647.
NextBioi83599.
PROiQ8TAP9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TAP9.
CleanExiHS_C7orf11.
ExpressionAtlasiQ8TAP9. baseline and differential.
GenevisibleiQ8TAP9. HS.

Family and domain databases

InterProiIPR028265. MPLKIP-like.
IPR026618. MPLKIP_like_vertebrate.
[Graphical view]
PANTHERiPTHR22446. PTHR22446. 1 hit.
PfamiPF15502. MPLKIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
  3. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  4. "TTDN1 is a Plk1-interacting protein involved in maintenance of cell cycle integrity."
    Zhang Y., Tian Y., Chen Q., Chen D., Zhai Z., Shu H.-B.
    Cell. Mol. Life Sci. 64:632-640(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PLK1, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-93; SER-104 AND THR-120, PHOSPHORYLATION AT SER-93 AND SER-104.
  5. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72 AND SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; THR-51; SER-80; SER-93; SER-104; THR-120 AND SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-80; SER-82 AND SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: VARIANT TTDN1 VAL-144, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  9. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLU-29.

Entry informationi

Entry nameiMPLKI_HUMAN
AccessioniPrimary (citable) accession number: Q8TAP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2002
Last modified: June 24, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.