Q8TAP9 (MPLKI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
M-phase-specific PLK1-interacting protein

Alternative name(s):
TTD non-photosensitive 1 protein

Gene names

Name:	MPLKIP
Synonyms:	C7orf11, TTDN1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in maintenance of cell cycle integrity by regulating mitosis or cytokinesis. Ref.4
Subunit structure	Interacts with PLK1; phosphorylation-dependent. Ref.4
Subcellular location	Nucleus. Cytoplasm. Cytoplasm › cytoskeleton › centrosome. Note: The subcellular location is regulated during cell cycle. During interphase located in the nucleus. During mitosis located at the centrosome and dispersed in the cytoplasm. During telophase located in the midbody. Colocalizes with PLK1 at the centrosome in M phase. Ref.4 Ref.8
Tissue specificity	Expressed at highest levels in liver and kidney; intermediate expression in skeletal muscle, pancreas, heart and placenta; low expression in brain and lung. Expressed in epidermis and hair follicles. Ref.2 Ref.8
Post-translational modification	Phosphorylated during mitosis in the cell cycle probably by CDK1. Ref.4
Involvement in disease	Trichothiodystrophy non-photosensitive 1 (TTDN1) [MIM:234050]: TTDN1 is an autosomal recessive disease characterized by sulfur-deficient brittle hair, intellectual impairment, decreased male fertility and no cutaneous photosensitivity. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Nucleus
Coding sequence diversity	Polymorphism
Disease	Disease mutation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	centrosome Inferred from direct assay Ref.4. Source: UniProtKB cytoplasm Inferred from direct assay Ref.4. Source: UniProtKB midbody Inferred from direct assay Ref.4. Source: UniProtKB nucleus Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	M-phase-specific PLK1-interacting protein		PRO_0000065674
Amino acid modifications
	Modified residue	47	1	Phosphoserine Ref.6
	Modified residue	51	1	Phosphothreonine Ref.6
	Modified residue	72	1	Phosphoserine Ref.5 Ref.7
	Modified residue	80	1	Phosphoserine Ref.6 Ref.7
	Modified residue	82	1	Phosphoserine Ref.7
	Modified residue	93	1	Phosphoserine Ref.4 Ref.6
	Modified residue	104	1	Phosphoserine Ref.4 Ref.6
	Modified residue	120	1	Phosphothreonine Ref.6
	Modified residue	124	1	Phosphoserine Ref.6
	Modified residue	133	1	Phosphoserine Ref.3 Ref.5 Ref.7
Natural variations
	Natural variant	29	1	G → E in a breast cancer sample; somatic mutation. Ref.9		VAR_036273
	Natural variant	144	1	M → V in TTDN1. Ref.8		VAR_022940
Experimental info
	Mutagenesis	93	1	S → A: Partially prevents phosphorylation. Ref.4
	Mutagenesis	104	1	S → A: Has no effect on interaction with PLK1 in mitosis. Partially prevents phosphorylation. Ref.4
	Mutagenesis	120	1	T → A: Abolishes interaction with PLK1 in mitosis. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8TAP9 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 77DBA4FBDF9C70CC	FASTA	179	19,147
	10 20 30 40 50 60 MQRQNFRPPT PPYPGPGGGG WGSGSSFRGT PGGGGPRPPS PRDGYGSPHH TPPYGPRSRP 70 80 90 100 110 120 YGSSHSPRHG GSFPGGRFGS PSPGGYPGSY SRSPAGSQQQ FGYSPGQQQT HPQGSPRTST 130 140 150 160 170 PFGSGRVREK RMSNELENYF KPSMLEDPWA GLEPVSVVDI SQQYSNTQTF TGKKGRYFC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"Molecular genetic studies of human chromosome 7 in Russell-Silver syndrome." Nakabayashi K., Fernandez B.A., Teshima I., Shuman C., Proud V.K., Curry C.J., Chitayat D., Grebe T., Ming J., Oshimura M., Meguro M., Mitsuya K., Deb-Rinker P., Herbrick J.A., Weksberg R., Scherer S.W. Genomics 79:186-196(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[3]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[4]	"TTDN1 is a Plk1-interacting protein involved in maintenance of cell cycle integrity." Zhang Y., Tian Y., Chen Q., Chen D., Zhai Z., Shu H.-B. Cell. Mol. Life Sci. 64:632-640(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PLK1, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-93; SER-104 AND THR-120, PHOSPHORYLATION AT SER-93 AND SER-104.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72 AND SER-133, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; THR-51; SER-80; SER-93; SER-104; THR-120 AND SER-124, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-80; SER-82 AND SER-133, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Identification of C7orf11 (TTDN1) gene mutations and genetic heterogeneity in nonphotosensitive trichothiodystrophy." Nakabayashi K., Amann D., Ren Y., Saarialho-Kere U., Avidan N., Gentles S., MacDonald J.R., Puffenberger E.G., Christiano A.M., Martinez-Mir A., Salas-Alanis J.C., Rizzo R., Vamos E., Raams A., Les C., Seboun E., Jaspers N.G.J., Beckmann J.S., Jackson C.E., Scherer S.W. Am. J. Hum. Genet. 76:510-516(2005) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT TTDN1 VAL-144, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLU-29.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC026265 mRNA. Translation: AAH26265.1.
IPI	IPI00152083.
RefSeq	NP_619646.1. NM_138701.3.
UniGene	Hs.654989.
3D structure databases
ProteinModelPortal	Q8TAP9.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000304553.
PTM databases
PhosphoSite	Q8TAP9.
Polymorphism databases
DMDM	71153365.
Proteomic databases
PaxDb	Q8TAP9.
PRIDE	Q8TAP9.
Protocols and materials databases
DNASU	136647.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306984; ENSP00000304553; ENSG00000168303.
GeneID	136647.
KEGG	hsa:136647.
UCSC	uc003thl.4. human.
Organism-specific databases
CTD	136647.
GeneCards	GC07M040172.
HGNC	HGNC:16002. MPLKIP.
MIM	234050. phenotype. 609188. gene.
neXtProt	NX_Q8TAP9.
Orphanet	1245. BIDS syndrome.
PharmGKB	PA25943.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG45065.
HOGENOM	HOG000252982.
HOVERGEN	HBG073319.
InParanoid	Q8TAP9.
OMA	SYSKSPA.
OrthoDB	EOG4WWRM0.
PhylomeDB	Q8TAP9.
Gene expression databases
ArrayExpress	Q8TAP9.
Bgee	Q8TAP9.
CleanEx	HS_C7orf11.
Genevestigator	Q8TAP9.
GermOnline	ENSG00000168303. Homo sapiens.
Family and domain databases
InterPro	IPR026618. MPLKIP. [Graphical view]
PANTHER	PTHR22446. PTHR22446. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	MPLKIP. human.
GenomeRNAi	136647.
NextBio	83599.
SOURCE	Search...

Entry information

Entry name

MPLKI_HUMAN

Accession

Primary (citable) accession number: Q8TAP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.