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Q8TAP9 (MPLKI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
M-phase-specific PLK1-interacting protein
Alternative name(s):
TTD non-photosensitive 1 protein
Gene names
Name:MPLKIP
Synonyms:C7orf11, TTDN1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length179 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in maintenance of cell cycle integrity by regulating mitosis or cytokinesis. Ref.4

Subunit structure

Interacts with PLK1; phosphorylation-dependent. Ref.4

Subcellular location

Nucleus. Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Note: The subcellular location is regulated during cell cycle. During interphase located in the nucleus. During mitosis located at the centrosome and dispersed in the cytoplasm. During telophase located in the midbody. Colocalizes with PLK1 at the centrosome in M phase. Ref.4 Ref.8

Tissue specificity

Expressed at highest levels in liver and kidney; intermediate expression in skeletal muscle, pancreas, heart and placenta; low expression in brain and lung. Expressed in epidermis and hair follicles. Ref.2 Ref.8

Post-translational modification

Phosphorylated during mitosis in the cell cycle probably by CDK1. Ref.4

Involvement in disease

Trichothiodystrophy non-photosensitive 1 (TTDN1) [MIM:234050]: TTDN1 is an autosomal recessive disease characterized by sulfur-deficient brittle hair, intellectual impairment, decreased male fertility and no cutaneous photosensitivity.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentCytoplasm
Cytoskeleton
Nucleus
   Coding sequence diversityPolymorphism
   DiseaseDisease mutation
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmitosis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcentrosome

Inferred from direct assay Ref.4. Source: UniProtKB

cytoplasm

Inferred from direct assay Ref.4. Source: UniProtKB

midbody

Inferred from direct assay Ref.4. Source: UniProtKB

nucleus

Inferred from direct assay Ref.4. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 179179M-phase-specific PLK1-interacting protein
PRO_0000065674

Amino acid modifications

Modified residue471Phosphoserine Ref.6
Modified residue511Phosphothreonine Ref.6
Modified residue721Phosphoserine Ref.5 Ref.7
Modified residue801Phosphoserine Ref.6 Ref.7
Modified residue821Phosphoserine Ref.7
Modified residue931Phosphoserine Ref.4 Ref.6
Modified residue1041Phosphoserine Ref.4 Ref.6
Modified residue1201Phosphothreonine Ref.6
Modified residue1241Phosphoserine Ref.6
Modified residue1331Phosphoserine Ref.3 Ref.5 Ref.7

Natural variations

Natural variant291G → E in a breast cancer sample; somatic mutation. Ref.9
VAR_036273
Natural variant1441M → V in TTDN1. Ref.8
VAR_022940

Experimental info

Mutagenesis931S → A: Partially prevents phosphorylation. Ref.4
Mutagenesis1041S → A: Has no effect on interaction with PLK1 in mitosis. Partially prevents phosphorylation. Ref.4
Mutagenesis1201T → A: Abolishes interaction with PLK1 in mitosis. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8TAP9 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 77DBA4FBDF9C70CC

FASTA17919,147
        10         20         30         40         50         60 
MQRQNFRPPT PPYPGPGGGG WGSGSSFRGT PGGGGPRPPS PRDGYGSPHH TPPYGPRSRP 

        70         80         90        100        110        120 
YGSSHSPRHG GSFPGGRFGS PSPGGYPGSY SRSPAGSQQQ FGYSPGQQQT HPQGSPRTST 

       130        140        150        160        170 
PFGSGRVREK RMSNELENYF KPSMLEDPWA GLEPVSVVDI SQQYSNTQTF TGKKGRYFC 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[2]"Molecular genetic studies of human chromosome 7 in Russell-Silver syndrome."
Nakabayashi K., Fernandez B.A., Teshima I., Shuman C., Proud V.K., Curry C.J., Chitayat D., Grebe T., Ming J., Oshimura M., Meguro M., Mitsuya K., Deb-Rinker P., Herbrick J.A., Weksberg R., Scherer S.W.
Genomics 79:186-196(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[3]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[4]"TTDN1 is a Plk1-interacting protein involved in maintenance of cell cycle integrity."
Zhang Y., Tian Y., Chen Q., Chen D., Zhai Z., Shu H.-B.
Cell. Mol. Life Sci. 64:632-640(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PLK1, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-93; SER-104 AND THR-120, PHOSPHORYLATION AT SER-93 AND SER-104.
[5]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72 AND SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; THR-51; SER-80; SER-93; SER-104; THR-120 AND SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-80; SER-82 AND SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Identification of C7orf11 (TTDN1) gene mutations and genetic heterogeneity in nonphotosensitive trichothiodystrophy."
Nakabayashi K., Amann D., Ren Y., Saarialho-Kere U., Avidan N., Gentles S., MacDonald J.R., Puffenberger E.G., Christiano A.M., Martinez-Mir A., Salas-Alanis J.C., Rizzo R., Vamos E., Raams A., Les C., Seboun E., Jaspers N.G.J., Beckmann J.S., Jackson C.E., Scherer S.W.
Am. J. Hum. Genet. 76:510-516(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT TTDN1 VAL-144, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] GLU-29.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC026265 mRNA. Translation: AAH26265.1.
RefSeqNP_619646.1. NM_138701.3.
UniGeneHs.654989.

3D structure databases

ProteinModelPortalQ8TAP9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126463. 14 interactions.
STRING9606.ENSP00000304553.

PTM databases

PhosphoSiteQ8TAP9.

Polymorphism databases

DMDM71153365.

Proteomic databases

PaxDbQ8TAP9.
PRIDEQ8TAP9.

Protocols and materials databases

DNASU136647.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000306984; ENSP00000304553; ENSG00000168303.
GeneID136647.
KEGGhsa:136647.
UCSCuc003thl.4. human.

Organism-specific databases

CTD136647.
GeneCardsGC07M040172.
HGNCHGNC:16002. MPLKIP.
HPAHPA058457.
MIM234050. phenotype.
609188. gene.
neXtProtNX_Q8TAP9.
Orphanet33364. Trichothiodystrophy.
PharmGKBPA25943.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45065.
HOGENOMHOG000252982.
HOVERGENHBG073319.
InParanoidQ8TAP9.
OMASYSKSPA.
OrthoDBEOG7BP84Z.
PhylomeDBQ8TAP9.
TreeFamTF335586.

Gene expression databases

ArrayExpressQ8TAP9.
BgeeQ8TAP9.
CleanExHS_C7orf11.
GenevestigatorQ8TAP9.

Family and domain databases

InterProIPR026618. MPLKIP.
IPR028265. MPLKIP-like.
[Graphical view]
PANTHERPTHR22446. PTHR22446. 1 hit.
PfamPF15502. MPLKIP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMPLKIP. human.
GeneWikiC7orf11.
MPLKIP.
GenomeRNAi136647.
NextBio83599.
PROQ8TAP9.
SOURCESearch...

Entry information

Entry nameMPLKI_HUMAN
AccessionPrimary (citable) accession number: Q8TAP9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2002
Last modified: March 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM