Reviewed, UniProtKB/Swiss-Prot Q8TAP6 (CEP76_HUMAN)

Names and origin

Protein names

Recommended name:
    Centrosomal protein of 76 kDa
      Short name=Cep76

Gene names

Name:	CEP76
Synonyms:	C18orf9

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	659 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication. Ref.10
Subunit structure	Interacts with CEP110/CP110 and CEP97. Ref.10
Subcellular location	Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › centrosome › centriole. Note: Does not localize along the ciliary axoneme. Ref.10 Ref.7
Developmental stage	Expressed at low level in G1 and is induced in S and G2 phase, during which point centrioles have already commenced duplication (at protein level). Ref.10
Sequence similarities	Belongs to the CEP76 family.
Sequence caution	The sequence BAG64223.1 differs from that shown. Reason: Erroneous termination at position 648. Translated as Trp.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of centriole replication Ref.10 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	centriole Ref.10 Inferred from direct assay. Source: UniProtKB cytosol Inferred from Experiment. Source: Reactome
Molecular function	protein binding Ref.10 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TAP6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TAP6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     99-174: TNIDPTRRYL...LLEVHRESLG → S

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8TAP6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-178: Missing.
     431-445: YIHKPTNPDEPPVAE → LCFQPSDVPGKLSTL
     446-659: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 659	659	Centrosomal protein of 76 kDa		PRO_0000089501
Amino acid modifications
	Modified residue	83	1	Phosphoserine Ref.8 Ref.9
Natural variations
	Alternative sequence	1 – 178	178	Missing in isoform 3.		VSP_037610
	Alternative sequence	99 – 174	76	TNIDP…RESLG → S in isoform 2.		VSP_037611
	Alternative sequence	431 – 445	15	YIHKP…PPVAE → LCFQPSDVPGKLSTL in isoform 3.		VSP_037612
	Alternative sequence	446 – 659	214	Missing in isoform 3.		VSP_037613
Experimental info
	Sequence conflict	314	1	N → S in BAB14123. Ref.1
	Sequence conflict	606	1	R → G in BAB14123. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 66EB85DCDCBEE518	FASTA	659	74,413
	10 20 30 40 50 60 MSLPPEKASE LKQLIHQQLS KMDVHGRIRE ILAETIREEL APDQQHLSTE DLIKALRRRG 70 80 90 100 110 120 IIDDVMKELN FVTDSVEQEL PSSPKQPICF DRQSTLKKTN IDPTRRYLYL QVLGGKAFLE 130 140 150 160 170 180 HLQEPEPLPG QVCSTFTLCL HYRNQRFRSK PVPCACEPDF HDGFLLEVHR ESLGDGTRMA 190 200 210 220 230 240 DSTTMLSISD PIHMVLIKTD IFGETTLVAS YFLEWRSVLG SENGVTSLTV ELMGVGTESK 250 260 270 280 290 300 VSVGILNIKL EMYPPLNQTL SQEVVNTQLA LERQKTAEKE RLFLVYAKQW WREYLQIRPS 310 320 330 340 350 360 HNSRLVKIFA QDENGINRPV CSYVKPLRAG RLLDTPRQAA RFVNVLGYER APVIGGGGKQ 370 380 390 400 410 420 EQWCTLLAFL CRNKGDCEDH ANLLCSLLLG YGLEAFVCVG TKAKGVPHAW VMTCGTDGAI 430 440 450 460 470 480 TFWESLTGHR YIHKPTNPDE PPVAEQPKPL YPYRTIGCVF NHQMFLGNCQ PSDAVETCVF 490 500 510 520 530 540 DLNDESKWKP MSEEAIKSVC APGATTSLPP FPPLCASTID ASVTSNEIEM QLRLLVSEHR 550 560 570 580 590 600 KDLGLTTVWE DQLSYLLSPA LASYEFERTT SISAGNEEFQ DAIRRAVPDG HTFKGFPIHF 610 620 630 640 650 VYRNARRAFA TCLRSPFCEE IICCRGDQVR LAVRVRVFTY PESACAVWIM FACKYRSVL
	Isoform 2. Checksum: 32792B1A54D92636	FASTA	584	65,733
	10 20 30 40 50 60 MSLPPEKASE LKQLIHQQLS KMDVHGRIRE ILAETIREEL APDQQHLSTE DLIKALRRRG 70 80 90 100 110 120 IIDDVMKELN FVTDSVEQEL PSSPKQPICF DRQSTLKKSD GTRMADSTTM LSISDPIHMV 130 140 150 160 170 180 LIKTDIFGET TLVASYFLEW RSVLGSENGV TSLTVELMGV GTESKVSVGI LNIKLEMYPP 190 200 210 220 230 240 LNQTLSQEVV NTQLALERQK TAEKERLFLV YAKQWWREYL QIRPSHNSRL VKIFAQDENG 250 260 270 280 290 300 INRPVCSYVK PLRAGRLLDT PRQAARFVNV LGYERAPVIG GGGKQEQWCT LLAFLCRNKG 310 320 330 340 350 360 DCEDHANLLC SLLLGYGLEA FVCVGTKAKG VPHAWVMTCG TDGAITFWES LTGHRYIHKP 370 380 390 400 410 420 TNPDEPPVAE QPKPLYPYRT IGCVFNHQMF LGNCQPSDAV ETCVFDLNDE SKWKPMSEEA 430 440 450 460 470 480 IKSVCAPGAT TSLPPFPPLC ASTIDASVTS NEIEMQLRLL VSEHRKDLGL TTVWEDQLSY 490 500 510 520 530 540 LLSPALASYE FERTTSISAG NEEFQDAIRR AVPDGHTFKG FPIHFVYRNA RRAFATCLRS 550 560 570 580 PFCEEIICCR GDQVRLAVRV RVFTYPESAC AVWIMFACKY RSVL
	Isoform 3. Checksum: 3DB77E46396240EF	FASTA	267	29,669
	10 20 30 40 50 60 MADSTTMLSI SDPIHMVLIK TDIFGETTLV ASYFLEWRSV LGSENGVTSL TVELMGVGTE 70 80 90 100 110 120 SKVSVGILNI KLEMYPPLNQ TLSQEVVNTQ LALERQKTAE KERLFLVYAK QWWREYLQIR 130 140 150 160 170 180 PSHNSRLVKI FAQDENGINR PVCSYVKPLR AGRLLDTPRQ AARFVNVLGY ERAPVIGGGG 190 200 210 220 230 240 KQEQWCTLLA FLCRNKGDCE DHANLLCSLL LGYGLEAFVC VGTKAKGVPH AWVMTCGTDG 250 260 AITFWESLTG HRLCFQPSDV PGKLSTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Teratocarcinoma, Testis and Thymus.
[2]	NHLBI resequencing and genotyping service (RS&G) Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed: 16177791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-73. Tissue: Stomach.
[7]	"Proteomic characterization of the human centrosome by protein correlation profiling." Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M. Nature 426:570-574(2003) [PubMed: 14654843] [Abstract] Cited for: MASS SPECTROMETRY, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, MASS SPECTROMETRY.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, MASS SPECTROMETRY.
[10]	"Cep76, a centrosomal protein that specifically restrains centriole reduplication." Tsang W.Y., Spektor A., Vijayakumar S., Bista B.R., Li J., Sanchez I., Duensing S., Dynlacht B.D. Dev. Cell 16:649-660(2009) [PubMed: 19460342] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH CEP110 AND CEP97.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, MASS SPECTROMETRY. Tissue: T-cell.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK022604 mRNA. Translation: BAB14123.1. AK301964 mRNA. Translation: BAG63377.1. AK303117 mRNA. Translation: BAG64223.1. Sequence problems. EF445040 Genomic DNA. Translation: ACA06091.1. AP005482 Genomic DNA. No translation available. CH471113 Genomic DNA. Translation: EAX01543.1. BC026307 mRNA. Translation: AAH26307.1. AL833727 mRNA. Translation: CAH56252.1.
IPI	IPI00154553. IPI00640272. IPI00642360.
RefSeq	NP_079175.2.
UniGene	Hs.236940
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TAP6. 8 interactions.
STRING	Q8TAP6.
PTM databases
PhosphoSite	Q8TAP6.
Proteomic databases
PRIDE	Q8TAP6.
Genome annotation databases
Ensembl	ENST00000262127; ENSP00000262127; ENSG00000101624; Homo sapiens. [Genome view]
GeneID	79959.
KEGG	hsa:79959.
UCSC	uc002kri.1. human.
Organism-specific databases
CTD	79959.
GeneCards	GC18M012663.
H-InvDB	HIX0021692.
HGNC	HGNC:25727. CEP76.
PharmGKB	PA134916911.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8TAP6.
HOVERGEN	Q8TAP6.
OMA	ACAVWIM
OrthoDB	EOG9QJV6V
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic.
Gene expression databases
ArrayExpress	Q8TAP6.
Bgee	Q8TAP6.
CleanEx	HS_CEP76.
Genevestigator	Q8TAP6.
GermOnline	ENSG00000101624. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	69951.

Entry information

Entry name

CEP76_HUMAN

Accession

Primary (citable) accession number: Q8TAP6
Secondary accession number(s): B0YJB2 , B4DXG5, B4DZW1, Q658N5, Q9H9U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	June 1, 2002
Last modified:	November 24, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.