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Protein

WD repeat-containing protein 48

Gene

WDR48

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 and USP46 (PubMed:18082604, PubMed:19075014, PubMed:26388029). Enhances the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself (PubMed:18082604). Also activates deubiquitinating activity of complexes containing USP12 (PubMed:19075014). Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1.4 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000114742-MONOMER.
ReactomeiR-HSA-5689880. Ub-specific processing proteases.
SignaLinkiQ8TAF3.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein 48
Alternative name(s):
USP1-associated factor 1
WD repeat endosomal protein
p801 Publication
Gene namesi
Name:WDR48
Synonyms:KIAA1449, UAF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:30914. WDR48.

Subcellular locationi

GO - Cellular componenti

  • late endosome Source: UniProtKB-SubCell
  • lysosome Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Lysosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi170S → Y: Strongly reduces interaction with USP46 and abolishes stimulation of USP46 enzyme activity. 1 Publication1
Mutagenesisi214K → E: Strongly reduces interaction with USP46 and abolishes stimulation of USP46 enzyme activity; when associated with A-256 and D-272. 1 Publication1
Mutagenesisi256W → A: Strongly reduces interaction with USP46 and abolishes stimulation of USP46 enzyme activity; when associated with E-214 and D-272. 1 Publication1
Mutagenesisi272R → D: Strongly reduces interaction with USP46 and abolishes stimulation of USP46 enzyme activity; when associated with E-214 and A-256. 1 Publication1

Organism-specific databases

DisGeNETi57599.
MalaCardsiWDR48.
OpenTargetsiENSG00000114742.
Orphaneti401800. Autosomal recessive spastic paraplegia type 60.
PharmGKBiPA134956949.

Polymorphism and mutation databases

BioMutaiWDR48.
DMDMi74760390.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000513991 – 677WD repeat-containing protein 48Add BLAST677

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphotyrosineBy similarity1
Modified residuei214N6-acetyllysineCombined sources1
Modified residuei578N6-acetyllysineBy similarity1
Modified residuei613PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8TAF3.
MaxQBiQ8TAF3.
PaxDbiQ8TAF3.
PeptideAtlasiQ8TAF3.
PRIDEiQ8TAF3.

PTM databases

iPTMnetiQ8TAF3.
PhosphoSitePlusiQ8TAF3.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000114742.
CleanExiHS_WDR48.
ExpressionAtlasiQ8TAF3. baseline and differential.
GenevisibleiQ8TAF3. HS.

Organism-specific databases

HPAiHPA038421.
HPA058015.

Interactioni

Subunit structurei

Interacts with USP46 (PubMed:26388029, PubMed:19075014). Interacts with USP1 (PubMed:18082604, PubMed:26388029). Interacts with USP12 (PubMed:19075014). Interacts with Saimiriine herpesvirus TIP protein (PubMed:12196293, PubMed:12885920). Interacts with papillomavirus HPV11 E1 protein (PubMed:18032488).6 Publications

Protein-protein interaction databases

BioGridi121649. 77 interactors.
DIPiDIP-50841N.
IntActiQ8TAF3. 64 interactors.
MINTiMINT-3043701.
STRINGi9606.ENSP00000307491.

Structurei

Secondary structure

1677
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 21Combined sources7
Beta strandi28 – 31Combined sources4
Beta strandi33 – 39Combined sources7
Turni40 – 43Combined sources4
Beta strandi44 – 49Combined sources6
Beta strandi54 – 58Combined sources5
Beta strandi67 – 71Combined sources5
Beta strandi78 – 84Combined sources7
Turni85 – 88Combined sources4
Beta strandi89 – 94Combined sources6
Beta strandi99 – 103Combined sources5
Turni104 – 107Combined sources4
Beta strandi108 – 113Combined sources6
Beta strandi120 – 126Combined sources7
Helixi127 – 129Combined sources3
Beta strandi131 – 136Combined sources6
Beta strandi141 – 145Combined sources5
Helixi146 – 150Combined sources5
Beta strandi154 – 156Combined sources3
Beta strandi158 – 161Combined sources4
Beta strandi162 – 164Combined sources3
Beta strandi171 – 176Combined sources6
Beta strandi183 – 187Combined sources5
Beta strandi191 – 195Combined sources5
Turni197 – 199Combined sources3
Beta strandi202 – 206Combined sources5
Beta strandi213 – 218Combined sources6
Beta strandi222 – 229Combined sources8
Beta strandi234 – 238Combined sources5
Turni239 – 242Combined sources4
Beta strandi243 – 248Combined sources6
Beta strandi255 – 260Combined sources6
Beta strandi266 – 271Combined sources6
Beta strandi275 – 282Combined sources8
Beta strandi287 – 292Combined sources6
Beta strandi297 – 302Combined sources6
Beta strandi305 – 308Combined sources4
Beta strandi310 – 319Combined sources10
Beta strandi321 – 325Combined sources5
Helixi329 – 334Combined sources6
Beta strandi354 – 357Combined sources4
Beta strandi363 – 368Combined sources6
Beta strandi372 – 379Combined sources8
Beta strandi384 – 388Combined sources5
Turni389 – 392Combined sources4
Beta strandi393 – 400Combined sources8
Helixi403 – 409Combined sources7
Beta strandi421 – 423Combined sources3
Beta strandi427 – 433Combined sources7
Turni435 – 439Combined sources5
Beta strandi442 – 444Combined sources3
Turni445 – 449Combined sources5
Beta strandi453 – 455Combined sources3
Beta strandi459 – 461Combined sources3
Helixi462 – 470Combined sources9
Turni471 – 473Combined sources3
Helixi475 – 477Combined sources3
Beta strandi514 – 522Combined sources9
Beta strandi524 – 529Combined sources6
Helixi530 – 534Combined sources5
Helixi536 – 545Combined sources10
Helixi548 – 554Combined sources7
Beta strandi564 – 571Combined sources8
Beta strandi583 – 588Combined sources6
Helixi593 – 603Combined sources11
Helixi631 – 638Combined sources8
Beta strandi639 – 643Combined sources5
Helixi654 – 660Combined sources7
Beta strandi664 – 674Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CVLX-ray3.00A2-580[»]
5CVNX-ray3.36A2-580[»]
5CVOX-ray3.88A/D1-677[»]
5K1AX-ray2.30B/D/F/H1-677[»]
5K1BX-ray3.30B1-677[»]
5K1CX-ray3.00B1-563[»]
5L8EX-ray2.30A/B9-580[»]
5L8WX-ray2.79B9-580[»]
ProteinModelPortaliQ8TAF3.
SMRiQ8TAF3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati28 – 67WD 1Add BLAST40
Repeati73 – 112WD 2Add BLAST40
Repeati115 – 154WD 3Add BLAST40
Repeati166 – 205WD 4Add BLAST40
Repeati208 – 247WD 5Add BLAST40
Repeati250 – 289WD 6Add BLAST40
Repeati292 – 334WD 7Add BLAST43
Repeati358 – 397WD 8Add BLAST40

Domaini

N-terminal WD region interacts with TIP and C-terminal region mediates lysosomal localization (Probable). The WD repeats are required for the interaction with deubiquitinating enzymes USP1, USP12 and USP46.Curated1 Publication

Sequence similaritiesi

Belongs to the WD repeat WDR48 family.Curated
Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0308. Eukaryota.
ENOG410XRJ5. LUCA.
GeneTreeiENSGT00820000127095.
HOVERGENiHBG079413.
InParanoidiQ8TAF3.
KOiK15361.
OMAiENEINHV.
OrthoDBiEOG091G03SS.
PhylomeDBiQ8TAF3.
TreeFamiTF315205.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR021772. DUF3337.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11816. DUF3337. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TAF3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAHHRQNTA GRRKVQVSYV IRDEVEKYNR NGVNALQLDP ALNRLFTAGR
60 70 80 90 100
DSIIRIWSVN QHKQDPYIAS MEHHTDWVND IVLCCNGKTL ISASSDTTVK
110 120 130 140 150
VWNAHKGFCM STLRTHKDYV KALAYAKDKE LVASAGLDRQ IFLWDVNTLT
160 170 180 190 200
ALTASNNTVT TSSLSGNKDS IYSLAMNQLG TIIVSGSTEK VLRVWDPRTC
210 220 230 240 250
AKLMKLKGHT DNVKALLLNR DGTQCLSGSS DGTIRLWSLG QQRCIATYRV
260 270 280 290 300
HDEGVWALQV NDAFTHVYSG GRDRKIYCTD LRNPDIRVLI CEEKAPVLKM
310 320 330 340 350
ELDRSADPPP AIWVATTKST VNKWTLKGIH NFRASGDYDN DCTNPITPLC
360 370 380 390 400
TQPDQVIKGG ASIIQCHILN DKRHILTKDT NNNVAYWDVL KACKVEDLGK
410 420 430 440 450
VDFEDEIKKR FKMVYVPNWF SVDLKTGMLT ITLDESDCFA AWVSAKDAGF
460 470 480 490 500
SSPDGSDPKL NLGGLLLQAL LEYWPRTHVN PMDEEENEVN HVNGEQENRV
510 520 530 540 550
QKGNGYFQVP PHTPVIFGEA GGRTLFRLLC RDSGGETESM LLNETVPQWV
560 570 580 590 600
IDITVDKNMP KFNKIPFYLQ PHASSGAKTL KKDRLSASDM LQVRKVMEHV
610 620 630 640 650
YEKIINLDNE SQTTSSSNNE KPGEQEKEED IAVLAEEKIE LLCQDQVLDP
660 670
NMDLRTVKHF IWKSGGDLTL HYRQKST
Length:677
Mass (Da):76,210
Last modified:June 1, 2002 - v1
Checksum:i20FDA620E02696E0
GO
Isoform 2 (identifier: Q8TAF3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-558: Missing.
     645-645: D → DQV

Note: No experimental confirmation available.
Show »
Length:121
Mass (Da):13,931
Checksum:iA1F40FA39E591BD9
GO
Isoform 3 (identifier: Q8TAF3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     91-99: Missing.

Note: No experimental confirmation available.
Show »
Length:668
Mass (Da):75,349
Checksum:i379C3527CC632FCF
GO
Isoform 4 (identifier: Q8TAF3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: MAAHHRQNTA...IVLCCNGKTL → MECQSAQV

Note: No experimental confirmation available.
Show »
Length:595
Mass (Da):66,707
Checksum:i3752FA8E527D8F7E
GO
Isoform 5 (identifier: Q8TAF3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     16-223: Missing.
     325-391: Missing.

Note: No experimental confirmation available.
Show »
Length:402
Mass (Da):45,552
Checksum:iF524F725C929EBD6
GO

Sequence cautioni

The sequence AAH37168 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA95973 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAH56182 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAH56300 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161T → A in AAH37168 (PubMed:15489334).Curated1
Sequence conflicti286I → V in BAG63646 (PubMed:14702039).Curated1
Sequence conflicti319S → F in BAG63646 (PubMed:14702039).Curated1
Sequence conflicti328G → E in BAG63646 (PubMed:14702039).Curated1
Sequence conflicti585L → P in BAG63646 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0167761 – 558Missing in isoform 2. 1 PublicationAdd BLAST558
Alternative sequenceiVSP_0376231 – 90MAAHH…NGKTL → MECQSAQV in isoform 4. 1 PublicationAdd BLAST90
Alternative sequenceiVSP_03762416 – 223Missing in isoform 5. 1 PublicationAdd BLAST208
Alternative sequenceiVSP_03762591 – 99Missing in isoform 3. 1 Publication9
Alternative sequenceiVSP_037626325 – 391Missing in isoform 5. 1 PublicationAdd BLAST67
Alternative sequenceiVSP_016777645D → DQV in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF468833 mRNA. Translation: AAL78650.1.
AB040882 mRNA. Translation: BAA95973.2. Different initiation.
AK297349 mRNA. Translation: BAG59798.1.
AK298810 mRNA. Translation: BAG60944.1.
AK302307 mRNA. Translation: BAG63646.1.
AL162064 mRNA. Translation: CAB82402.1.
AL832926 mRNA. Translation: CAH56300.1. Different initiation.
BX649170 mRNA. Translation: CAH56182.1. Different initiation.
BC026353 mRNA. Translation: AAH26353.1.
BC037168 mRNA. Translation: AAH37168.1. Different initiation.
CCDSiCCDS33738.1. [Q8TAF3-1]
PIRiT47168.
RefSeqiNP_001290331.1. NM_001303402.1. [Q8TAF3-4]
NP_001290332.1. NM_001303403.1. [Q8TAF3-3]
NP_065890.1. NM_020839.3. [Q8TAF3-1]
UniGeneiHs.109778.

Genome annotation databases

EnsembliENST00000302313; ENSP00000307491; ENSG00000114742. [Q8TAF3-1]
GeneIDi57599.
KEGGihsa:57599.
UCSCiuc003cit.4. human. [Q8TAF3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF468833 mRNA. Translation: AAL78650.1.
AB040882 mRNA. Translation: BAA95973.2. Different initiation.
AK297349 mRNA. Translation: BAG59798.1.
AK298810 mRNA. Translation: BAG60944.1.
AK302307 mRNA. Translation: BAG63646.1.
AL162064 mRNA. Translation: CAB82402.1.
AL832926 mRNA. Translation: CAH56300.1. Different initiation.
BX649170 mRNA. Translation: CAH56182.1. Different initiation.
BC026353 mRNA. Translation: AAH26353.1.
BC037168 mRNA. Translation: AAH37168.1. Different initiation.
CCDSiCCDS33738.1. [Q8TAF3-1]
PIRiT47168.
RefSeqiNP_001290331.1. NM_001303402.1. [Q8TAF3-4]
NP_001290332.1. NM_001303403.1. [Q8TAF3-3]
NP_065890.1. NM_020839.3. [Q8TAF3-1]
UniGeneiHs.109778.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CVLX-ray3.00A2-580[»]
5CVNX-ray3.36A2-580[»]
5CVOX-ray3.88A/D1-677[»]
5K1AX-ray2.30B/D/F/H1-677[»]
5K1BX-ray3.30B1-677[»]
5K1CX-ray3.00B1-563[»]
5L8EX-ray2.30A/B9-580[»]
5L8WX-ray2.79B9-580[»]
ProteinModelPortaliQ8TAF3.
SMRiQ8TAF3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121649. 77 interactors.
DIPiDIP-50841N.
IntActiQ8TAF3. 64 interactors.
MINTiMINT-3043701.
STRINGi9606.ENSP00000307491.

PTM databases

iPTMnetiQ8TAF3.
PhosphoSitePlusiQ8TAF3.

Polymorphism and mutation databases

BioMutaiWDR48.
DMDMi74760390.

Proteomic databases

EPDiQ8TAF3.
MaxQBiQ8TAF3.
PaxDbiQ8TAF3.
PeptideAtlasiQ8TAF3.
PRIDEiQ8TAF3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302313; ENSP00000307491; ENSG00000114742. [Q8TAF3-1]
GeneIDi57599.
KEGGihsa:57599.
UCSCiuc003cit.4. human. [Q8TAF3-1]

Organism-specific databases

CTDi57599.
DisGeNETi57599.
GeneCardsiWDR48.
HGNCiHGNC:30914. WDR48.
HPAiHPA038421.
HPA058015.
MalaCardsiWDR48.
MIMi612167. gene.
neXtProtiNX_Q8TAF3.
OpenTargetsiENSG00000114742.
Orphaneti401800. Autosomal recessive spastic paraplegia type 60.
PharmGKBiPA134956949.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0308. Eukaryota.
ENOG410XRJ5. LUCA.
GeneTreeiENSGT00820000127095.
HOVERGENiHBG079413.
InParanoidiQ8TAF3.
KOiK15361.
OMAiENEINHV.
OrthoDBiEOG091G03SS.
PhylomeDBiQ8TAF3.
TreeFamiTF315205.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000114742-MONOMER.
ReactomeiR-HSA-5689880. Ub-specific processing proteases.
SignaLinkiQ8TAF3.

Miscellaneous databases

ChiTaRSiWDR48. human.
GenomeRNAii57599.
PROiQ8TAF3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114742.
CleanExiHS_WDR48.
ExpressionAtlasiQ8TAF3. baseline and differential.
GenevisibleiQ8TAF3. HS.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR021772. DUF3337.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11816. DUF3337. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWDR48_HUMAN
AccessioniPrimary (citable) accession number: Q8TAF3
Secondary accession number(s): B4DM86
, B4DQI2, B4DY84, Q63HJ2, Q658Y1, Q8N3Z1, Q9NSK8, Q9P279
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.