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Q8TAE8 (G45IP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Growth arrest and DNA damage-inducible proteins-interacting protein 1
Alternative name(s):
39S ribosomal protein L59, mitochondrial
Short name=MRP-L59
CKII beta-associating protein
CR6-interacting factor 1
Short name=CRIF1
Papillomavirus L2-interacting nuclear protein 1
Short name=PLINP
Short name=PLINP-1
p53-responsive gene 6 protein
Gene names
Name:GADD45GIP1
Synonyms:MRPL59, PLINP1, PRG6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.

Subunit structure

Interacts with GADD45A, GADD45B and GADD45G. Interacts with NR4A1 via the NR4A1 AB domain. Interacts with the human papilloma virus type 16 (HPV 16) minor capsid protein L2. Interacts with ATAD3A and ATAD3B. Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins. Ref.1 Ref.2 Ref.6 Ref.10 Ref.11

Subcellular location

Mitochondrion. Nucleus. Note: Using N-terminally tagged constructs, has been found in the nucleus (Ref.1). C-terminally tagged constructs are targeted exclusively to mitochondria (Ref.10). This discrepancy may be explained by masking of a potential N-terminal mitochondrial targeting signal by the tag (Ref.10). Ref.1 Ref.2 Ref.10 Ref.11

Tissue specificity

Widely expressed. Highly expressed in the thyroid gland, heart, lymph nodes, trachea and adrenal tissues. Expressed at lower level in liver skeletal muscle, kidney, pancreas, testis, ovary and stomach. Barely detectable in adrenal adenoma and papillary thyroid cancer. Ref.2

Induction

Down-regulated by p53/TP53 in apoptotic cells. Ref.5

Miscellaneous

Cells overexpressing GADD45GIP1 were more likely to be in G1 and less likely to be in S phase and grow more slowly than control cells. Inhibiting the expression of GADD45GIP1 promotes cell cycle progression.

Ontologies

Keywords
   Biological processCell cycle
Host-virus interaction
   Cellular componentMitochondrion
Nucleus
   DomainCoiled coil
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction Ref.2PubMed 18200042. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GADD45AP245223EBI-372506,EBI-448167
Gadd45bP223393EBI-372506,EBI-2266938From a different organism.
GADD45GO952574EBI-372506,EBI-448202
STAT3P407634EBI-372506,EBI-518675

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 222222Growth arrest and DNA damage-inducible proteins-interacting protein 1
PRO_0000228620

Regions

Coiled coil99 – 212114 Potential
Motif184 – 20017Nuclear localization signal Potential
Compositional bias136 – 1394Poly-Gln

Experimental info

Sequence conflict7 – 104QARS → KALN in CAD22344. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8TAE8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 304C397AEC958804

FASTA22225,384
        10         20         30         40         50         60 
MAASVRQARS LLGVAATLAP GSRGYRARPP PRRRPGPRWP DPEDLLTPRW QLGPRYAAKQ 

        70         80         90        100        110        120 
FARYGAASGV VPGSLWPSPE QLRELEAEER EWYPSLATMQ ESLRVKQLAE EQKRREREQH 

       130        140        150        160        170        180 
IAECMAKMPQ MIVNWQQQQR ENWEKAQADK ERRARLQAEA QELLGYQVDP RSARFQELLQ 

       190        200        210        220 
DLEKKERKRL KEEKQKRKKE ARAAALAAAV AQDPAASGAP SS 

« Hide

References

« Hide 'large scale' references
[1]"Interaction of human papillomavirus type 16 L2 with cellular proteins: identification of novel nuclear body-associated proteins."
Goernemann J., Hofmann T.G., Will H., Mueller M.
Virology 303:69-78(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH HPV16 L2.
Tissue: Keratinocyte.
[2]"CR6-interacting factor 1 interacts with Gadd45 family proteins and modulates the cell cycle."
Chung H.K., Yi Y.-W., Jung N.-C., Kim D., Suh J.M., Kim H., Park K.C., Song J.H., Kim D.W., Hwang E.S., Yoon S.-H., Bae Y.-S., Kim J.M., Bae I., Shong M.
J. Biol. Chem. 278:28079-28088(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH GADD45A; GADD45B AND GADD45G, TISSUE SPECIFICITY.
Tissue: Colon carcinoma.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung, Muscle and Skin.
[4]"Full length sequencing of some human and murine muscular transcripts (Telethon Italy project B41)."
Frigimelica E., Lanfranchi G.
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-222.
[5]"Isolation of differentially expressed cDNAs from p53-dependent apoptotic cells: activation of the human homologue of the Drosophila peroxidasin gene."
Horikoshi N., Cong J., Kley N., Shenk T.
Biochem. Biophys. Res. Commun. 261:864-869(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 90-122, INDUCTION.
[6]"CR6-interacting factor 1 interacts with orphan nuclear receptor Nur77 and inhibits its transactivation."
Park K.C., Song K.-H., Chung H.K., Kim H., Kim D.W., Song J.H., Hwang E.S., Jung H.S., Park S.-H., Bae I., Lee I.K., Choi H.-S., Shong M.
Mol. Endocrinol. 19:12-24(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NR4A1/NUR77.
[7]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis."
He J., Cooper H.M., Reyes A., Di Re M., Sembongi H., Litwin T.R., Gao J., Neuman K.C., Fearnley I.M., Spinazzola A., Walker J.E., Holt I.J.
Nucleic Acids Res. 40:6109-6121(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATAD3A AND ATAD3B, SUBCELLULAR LOCATION.
[11]"Identification and characterization of CHCHD1, AURKAIP1, and CRIF1 as new members of the mammalian mitochondrial ribosome."
Koc E.C., Cimen H., Kumcuoglu B., Abu N., Akpinar G., Haque M.E., Spremulli L.L., Koc H.
Front. Physiol. 4:183-183(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ429498 mRNA. Translation: CAD22344.1.
AF479749 mRNA. Translation: AAL85877.1.
AF475095 mRNA. Translation: AAM10639.1.
BC004944 mRNA. Translation: AAH04944.1.
BC013039 mRNA. Translation: AAH13039.2.
BC013027 mRNA. Translation: AAH13027.2.
BC069200 mRNA. Translation: AAH69200.1.
AJ295986 mRNA. Translation: CAC82499.1.
AF147080 mRNA. No translation available.
CCDSCCDS12290.1.
RefSeqNP_443082.2. NM_052850.3.
UniGeneHs.515164.

3D structure databases

ProteinModelPortalQ8TAE8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124721. 24 interactions.
IntActQ8TAE8. 8 interactions.
MINTMINT-253595.
STRING9606.ENSP00000323065.

PTM databases

PhosphoSiteQ8TAE8.

Polymorphism databases

DMDM74730346.

Proteomic databases

MaxQBQ8TAE8.
PaxDbQ8TAE8.
PeptideAtlasQ8TAE8.
PRIDEQ8TAE8.

Protocols and materials databases

DNASU90480.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000316939; ENSP00000323065; ENSG00000179271.
GeneID90480.
KEGGhsa:90480.
UCSCuc002mwb.4. human.

Organism-specific databases

CTD90480.
GeneCardsGC19M013065.
HGNCHGNC:29996. GADD45GIP1.
MIM605162. gene.
neXtProtNX_Q8TAE8.
PharmGKBPA134954784.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG285057.
HOGENOMHOG000112686.
HOVERGENHBG068845.
InParanoidQ8TAE8.
OMAMPQMIEN.
OrthoDBEOG7ZKSDX.
PhylomeDBQ8TAE8.
TreeFamTF323794.

Gene expression databases

ArrayExpressQ8TAE8.
BgeeQ8TAE8.
CleanExHS_GADD45GIP1.
GenevestigatorQ8TAE8.

Family and domain databases

InterProIPR018472. Damage-induce-interacting_prot.
[Graphical view]
PfamPF10147. CR6_interact. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGADD45GIP1.
GenomeRNAi90480.
NextBio76780.
PROQ8TAE8.
SOURCESearch...

Entry information

Entry nameG45IP_HUMAN
AccessionPrimary (citable) accession number: Q8TAE8
Secondary accession number(s): Q8IVM3 expand/collapse secondary AC list , Q8TE51, Q969P9, Q9BSM6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM