Q8TAE8 (G45IP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Growth arrest and DNA damage-inducible proteins-interacting protein 1

Alternative name(s):
CKII beta-associating protein
CR6-interacting factor 1
Short name=CRIF1
Papillomavirus L2-interacting nuclear protein 1
Short name=PLINP
Short name=PLINP-1
p53-responsive gene 6 protein

Gene names

Name:	GADD45GIP1
Synonyms:	PLINP1, PRG6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.
Subunit structure	Interacts with GADD45A, GADD45B and GADD45G. Interacts with NR4A1 via the NR4A1 AB domain. Interacts with the human papilloma virus type 16 (HPV 16) minor capsid protein L2. Interacts with ATAD3A and ATAD3B. Ref.1 Ref.2 Ref.6 Ref.10
Subcellular location	Mitochondrion. Nucleus. Note: Using N-terminally tagged constructs, has been found in the nucleus (Ref.1). C-terminally tagged constructs are targeted exclusively to mitochondria (Ref.10). This discrepancy may be explained by masking of a potential N-terminal mitochondrial targeting signal by the tag (Ref.10). Ref.1 Ref.2 Ref.10
Tissue specificity	Widely expressed. Highly expressed in the thyroid gland, heart, lymph nodes, trachea and adrenal tissues. Expressed at lower level in liver skeletal muscle, kidney, pancreas, testis, ovary and stomach. Barely detectable in adrenal adenoma and papillary thyroid cancer. Ref.2
Induction	Down-regulated by p53/TP53 in apoptotic cells. Ref.5
Miscellaneous	Cells overexpressing GADD45GIP1 were more likely to be in G1 and less likely to be in S phase and grow more slowly than control cells. Inhibiting the expression of GADD45GIP1 promotes cell cycle progression.

Ontologies

Keywords
Biological process	Cell cycle Host-virus interaction
Cellular component	Mitochondrion Nucleus
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GADD45A	P24522	3	EBI-372506,EBI-448167
Gadd45b	P22339	3	EBI-372506,EBI-2266938	From a different organism.
GADD45G	O95257	4	EBI-372506,EBI-448202

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Growth arrest and DNA damage-inducible proteins-interacting protein 1		PRO_0000228620
Regions
	Coiled coil	99 – 212	114	Potential
	Motif	184 – 200	17	Nuclear localization signal Potential
	Compositional bias	136 – 139	4	Poly-Gln
Experimental info
	Sequence conflict	7 – 10	4	QARS → KALN in CAD22344. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8TAE8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 304C397AEC958804	FASTA	222	25,384
	10 20 30 40 50 60 MAASVRQARS LLGVAATLAP GSRGYRARPP PRRRPGPRWP DPEDLLTPRW QLGPRYAAKQ 70 80 90 100 110 120 FARYGAASGV VPGSLWPSPE QLRELEAEER EWYPSLATMQ ESLRVKQLAE EQKRREREQH 130 140 150 160 170 180 IAECMAKMPQ MIVNWQQQQR ENWEKAQADK ERRARLQAEA QELLGYQVDP RSARFQELLQ 190 200 210 220 DLEKKERKRL KEEKQKRKKE ARAAALAAAV AQDPAASGAP SS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction of human papillomavirus type 16 L2 with cellular proteins: identification of novel nuclear body-associated proteins." Goernemann J., Hofmann T.G., Will H., Mueller M. Virology 303:69-78(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH HPV16 L2. Tissue: Keratinocyte.
[2]	"CR6-interacting factor 1 interacts with Gadd45 family proteins and modulates the cell cycle." Chung H.K., Yi Y.-W., Jung N.-C., Kim D., Suh J.M., Kim H., Park K.C., Song J.H., Kim D.W., Hwang E.S., Yoon S.-H., Bae Y.-S., Kim J.M., Bae I., Shong M. J. Biol. Chem. 278:28079-28088(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH GADD45A; GADD45B AND GADD45G, TISSUE SPECIFICITY. Tissue: Colon carcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung, Muscle and Skin.
[4]	"Full length sequencing of some human and murine muscular transcripts (Telethon Italy project B41)." Frigimelica E., Lanfranchi G. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-222.
[5]	"Isolation of differentially expressed cDNAs from p53-dependent apoptotic cells: activation of the human homologue of the Drosophila peroxidasin gene." Horikoshi N., Cong J., Kley N., Shenk T. Biochem. Biophys. Res. Commun. 261:864-869(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 90-122, INDUCTION.
[6]	"CR6-interacting factor 1 interacts with orphan nuclear receptor Nur77 and inhibits its transactivation." Park K.C., Song K.-H., Chung H.K., Kim H., Kim D.W., Song J.H., Hwang E.S., Jung H.S., Park S.-H., Bae I., Lee I.K., Choi H.-S., Shong M. Mol. Endocrinol. 19:12-24(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NR4A1/NUR77.
[7]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis." He J., Cooper H.M., Reyes A., Di Re M., Sembongi H., Litwin T.R., Gao J., Neuman K.C., Fearnley I.M., Spinazzola A., Walker J.E., Holt I.J. Nucleic Acids Res. 40:6109-6121(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ATAD3A AND ATAD3B, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ429498 mRNA. Translation: CAD22344.1. AF479749 mRNA. Translation: AAL85877.1. AF475095 mRNA. Translation: AAM10639.1. BC004944 mRNA. Translation: AAH04944.1. BC013039 mRNA. Translation: AAH13039.2. BC013027 mRNA. Translation: AAH13027.2. BC069200 mRNA. Translation: AAH69200.1. AJ295986 mRNA. Translation: CAC82499.1. AF147080 mRNA. No translation available.
IPI	IPI00552587.
RefSeq	NP_443082.2. NM_052850.3.
UniGene	Hs.515164.
3D structure databases
ProteinModelPortal	Q8TAE8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TAE8. 5 interactions.
MINT	MINT-253595.
STRING	9606.ENSP00000323065.
PTM databases
PhosphoSite	Q8TAE8.
Polymorphism databases
DMDM	74730346.
Proteomic databases
PaxDb	Q8TAE8.
PeptideAtlas	Q8TAE8.
PRIDE	Q8TAE8.
Protocols and materials databases
DNASU	90480.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000316939; ENSP00000323065; ENSG00000179271.
GeneID	90480.
KEGG	hsa:90480.
UCSC	uc002mwb.3. human.
Organism-specific databases
CTD	90480.
GeneCards	GC19M013065.
HGNC	HGNC:29996. GADD45GIP1.
MIM	605162. gene.
neXtProt	NX_Q8TAE8.
PharmGKB	PA134954784.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG285057.
HOGENOM	HOG000112686.
HOVERGEN	HBG068845.
InParanoid	Q8TAE8.
OMA	MPQMIEN.
OrthoDB	EOG4CC42T.
PhylomeDB	Q8TAE8.
Gene expression databases
ArrayExpress	Q8TAE8.
Bgee	Q8TAE8.
CleanEx	HS_GADD45GIP1.
Genevestigator	Q8TAE8.
GermOnline	ENSG00000179271. Homo sapiens.
Family and domain databases
InterPro	IPR018472. Damage-induce-interacting_prot. [Graphical view]
Pfam	PF10147. CR6_interact. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	90480.
NextBio	76780.
SOURCE	Search...

Entry information

Entry name

G45IP_HUMAN

Accession

Primary (citable) accession number: Q8TAE8
Secondary accession number(s): Q8IVM3 , Q8TE51, Q969P9, Q9BSM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.