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Protein

Growth arrest and DNA damage-inducible proteins-interacting protein 1

Gene

GADD45GIP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Host-virus interaction

Enzyme and pathway databases

ReactomeiREACT_267634. Mitochondrial translation initiation.
REACT_268133. Mitochondrial translation elongation.
REACT_268261. Mitochondrial translation termination.

Names & Taxonomyi

Protein namesi
Recommended name:
Growth arrest and DNA damage-inducible proteins-interacting protein 1
Alternative name(s):
39S ribosomal protein L59, mitochondrial
Short name:
MRP-L59
CKII beta-associating protein
CR6-interacting factor 1
Short name:
CRIF1
Papillomavirus L2-interacting nuclear protein 1
Short name:
PLINP
Short name:
PLINP-1
p53-responsive gene 6 protein
Gene namesi
Name:GADD45GIP1
Synonyms:MRPL59, PLINP1, PRG6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:29996. GADD45GIP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134954784.

Polymorphism and mutation databases

DMDMi74730346.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Growth arrest and DNA damage-inducible proteins-interacting protein 1PRO_0000228620Add
BLAST

Proteomic databases

MaxQBiQ8TAE8.
PaxDbiQ8TAE8.
PeptideAtlasiQ8TAE8.
PRIDEiQ8TAE8.

PTM databases

PhosphoSiteiQ8TAE8.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in the thyroid gland, heart, lymph nodes, trachea and adrenal tissues. Expressed at lower level in liver skeletal muscle, kidney, pancreas, testis, ovary and stomach. Barely detectable in adrenal adenoma and papillary thyroid cancer.1 Publication

Inductioni

Down-regulated by p53/TP53 in apoptotic cells.1 Publication

Gene expression databases

BgeeiQ8TAE8.
CleanExiHS_GADD45GIP1.
GenevisibleiQ8TAE8. HS.

Organism-specific databases

HPAiHPA055205.
HPA065048.

Interactioni

Subunit structurei

Interacts with GADD45A, GADD45B and GADD45G. Interacts with NR4A1 via the NR4A1 AB domain. Interacts with the human papilloma virus type 16 (HPV 16) minor capsid protein L2. Interacts with ATAD3A and ATAD3B. Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GADD45AP245223EBI-372506,EBI-448167
Gadd45bP223393EBI-372506,EBI-2266938From a different organism.
GADD45GO952574EBI-372506,EBI-448202
STAT3P407634EBI-372506,EBI-518675
Stat3P422273EBI-372506,EBI-602878From a different organism.

Protein-protein interaction databases

BioGridi124721. 35 interactions.
IntActiQ8TAE8. 9 interactions.
MINTiMINT-253595.
STRINGi9606.ENSP00000323065.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J7Yelectron microscopy3.40q1-222[»]
3J9Melectron microscopy3.50q1-222[»]
ProteinModelPortaliQ8TAE8.
SMRiQ8TAE8. Positions 43-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili99 – 212114Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi184 – 20017Nuclear localization signalSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi136 – 1394Poly-Gln

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG285057.
GeneTreeiENSGT00390000013719.
HOGENOMiHOG000112686.
HOVERGENiHBG068845.
InParanoidiQ8TAE8.
OMAiEQHIAEC.
OrthoDBiEOG7ZKSDX.
PhylomeDBiQ8TAE8.
TreeFamiTF323794.

Family and domain databases

InterProiIPR018472. Damage-induce-interacting_prot.
[Graphical view]
PfamiPF10147. CR6_interact. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TAE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVRQARS LLGVAATLAP GSRGYRARPP PRRRPGPRWP DPEDLLTPRW
60 70 80 90 100
QLGPRYAAKQ FARYGAASGV VPGSLWPSPE QLRELEAEER EWYPSLATMQ
110 120 130 140 150
ESLRVKQLAE EQKRREREQH IAECMAKMPQ MIVNWQQQQR ENWEKAQADK
160 170 180 190 200
ERRARLQAEA QELLGYQVDP RSARFQELLQ DLEKKERKRL KEEKQKRKKE
210 220
ARAAALAAAV AQDPAASGAP SS
Length:222
Mass (Da):25,384
Last modified:June 1, 2002 - v1
Checksum:i304C397AEC958804
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 104QARS → KALN in CAD22344 (PubMed:12482659).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ429498 mRNA. Translation: CAD22344.1.
AF479749 mRNA. Translation: AAL85877.1.
AF475095 mRNA. Translation: AAM10639.1.
BC004944 mRNA. Translation: AAH04944.1.
BC013039 mRNA. Translation: AAH13039.2.
BC013027 mRNA. Translation: AAH13027.2.
BC069200 mRNA. Translation: AAH69200.1.
AJ295986 mRNA. Translation: CAC82499.1.
AF147080 mRNA. No translation available.
CCDSiCCDS12290.1.
RefSeqiNP_443082.2. NM_052850.3.
UniGeneiHs.515164.

Genome annotation databases

EnsembliENST00000316939; ENSP00000323065; ENSG00000179271.
GeneIDi90480.
KEGGihsa:90480.
UCSCiuc002mwb.4. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ429498 mRNA. Translation: CAD22344.1.
AF479749 mRNA. Translation: AAL85877.1.
AF475095 mRNA. Translation: AAM10639.1.
BC004944 mRNA. Translation: AAH04944.1.
BC013039 mRNA. Translation: AAH13039.2.
BC013027 mRNA. Translation: AAH13027.2.
BC069200 mRNA. Translation: AAH69200.1.
AJ295986 mRNA. Translation: CAC82499.1.
AF147080 mRNA. No translation available.
CCDSiCCDS12290.1.
RefSeqiNP_443082.2. NM_052850.3.
UniGeneiHs.515164.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J7Yelectron microscopy3.40q1-222[»]
3J9Melectron microscopy3.50q1-222[»]
ProteinModelPortaliQ8TAE8.
SMRiQ8TAE8. Positions 43-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124721. 35 interactions.
IntActiQ8TAE8. 9 interactions.
MINTiMINT-253595.
STRINGi9606.ENSP00000323065.

PTM databases

PhosphoSiteiQ8TAE8.

Polymorphism and mutation databases

DMDMi74730346.

Proteomic databases

MaxQBiQ8TAE8.
PaxDbiQ8TAE8.
PeptideAtlasiQ8TAE8.
PRIDEiQ8TAE8.

Protocols and materials databases

DNASUi90480.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316939; ENSP00000323065; ENSG00000179271.
GeneIDi90480.
KEGGihsa:90480.
UCSCiuc002mwb.4. human.

Organism-specific databases

CTDi90480.
GeneCardsiGC19M013065.
HGNCiHGNC:29996. GADD45GIP1.
HPAiHPA055205.
HPA065048.
MIMi605162. gene.
neXtProtiNX_Q8TAE8.
PharmGKBiPA134954784.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG285057.
GeneTreeiENSGT00390000013719.
HOGENOMiHOG000112686.
HOVERGENiHBG068845.
InParanoidiQ8TAE8.
OMAiEQHIAEC.
OrthoDBiEOG7ZKSDX.
PhylomeDBiQ8TAE8.
TreeFamiTF323794.

Enzyme and pathway databases

ReactomeiREACT_267634. Mitochondrial translation initiation.
REACT_268133. Mitochondrial translation elongation.
REACT_268261. Mitochondrial translation termination.

Miscellaneous databases

GeneWikiiGADD45GIP1.
GenomeRNAii90480.
NextBioi76780.
PROiQ8TAE8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TAE8.
CleanExiHS_GADD45GIP1.
GenevisibleiQ8TAE8. HS.

Family and domain databases

InterProiIPR018472. Damage-induce-interacting_prot.
[Graphical view]
PfamiPF10147. CR6_interact. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Interaction of human papillomavirus type 16 L2 with cellular proteins: identification of novel nuclear body-associated proteins."
    Goernemann J., Hofmann T.G., Will H., Mueller M.
    Virology 303:69-78(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH HPV16 L2.
    Tissue: Keratinocyte.
  2. "CR6-interacting factor 1 interacts with Gadd45 family proteins and modulates the cell cycle."
    Chung H.K., Yi Y.-W., Jung N.-C., Kim D., Suh J.M., Kim H., Park K.C., Song J.H., Kim D.W., Hwang E.S., Yoon S.-H., Bae Y.-S., Kim J.M., Bae I., Shong M.
    J. Biol. Chem. 278:28079-28088(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH GADD45A; GADD45B AND GADD45G, TISSUE SPECIFICITY.
    Tissue: Colon carcinoma.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung, Muscle and Skin.
  4. "Full length sequencing of some human and murine muscular transcripts (Telethon Italy project B41)."
    Frigimelica E., Lanfranchi G.
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-222.
  5. "Isolation of differentially expressed cDNAs from p53-dependent apoptotic cells: activation of the human homologue of the Drosophila peroxidasin gene."
    Horikoshi N., Cong J., Kley N., Shenk T.
    Biochem. Biophys. Res. Commun. 261:864-869(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 90-122, INDUCTION.
  6. "CR6-interacting factor 1 interacts with orphan nuclear receptor Nur77 and inhibits its transactivation."
    Park K.C., Song K.-H., Chung H.K., Kim H., Kim D.W., Song J.H., Hwang E.S., Jung H.S., Park S.-H., Bae I., Lee I.K., Choi H.-S., Shong M.
    Mol. Endocrinol. 19:12-24(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NR4A1/NUR77.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: INTERACTION WITH ATAD3A AND ATAD3B, SUBCELLULAR LOCATION.
  11. "Identification and characterization of CHCHD1, AURKAIP1, and CRIF1 as new members of the mammalian mitochondrial ribosome."
    Koc E.C., Cimen H., Kumcuoglu B., Abu N., Akpinar G., Haque M.E., Spremulli L.L., Koc H.
    Front. Physiol. 4:183-183(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiG45IP_HUMAN
AccessioniPrimary (citable) accession number: Q8TAE8
Secondary accession number(s): Q8IVM3
, Q8TE51, Q969P9, Q9BSM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: June 1, 2002
Last modified: June 24, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Cells overexpressing GADD45GIP1 were more likely to be in G1 and less likely to be in S phase and grow more slowly than control cells. Inhibiting the expression of GADD45GIP1 promotes cell cycle progression.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.