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Protein

Protein phosphatase 1 regulatory subunit 14C

Gene

PPP1R14C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of the PP1 regulatory subunit PPP1CA.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Enzyme and pathway databases

SIGNORiQ8TAE6.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 14C
Alternative name(s):
Kinase-enhanced PP1 inhibitor
PKC-potentiated PP1 inhibitory protein
Serologically defined breast cancer antigen NY-BR-81
Gene namesi
Name:PPP1R14C
Synonyms:KEPI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:14952. PPP1R14C.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33630.

Polymorphism and mutation databases

BioMutaiPPP1R14C.
DMDMi90110047.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 165164Protein phosphatase 1 regulatory subunit 14CPRO_0000071494Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei25 – 251PhosphoserineBy similarity
Modified residuei73 – 731Phosphothreonine; by ILK11 Publication

Post-translational modificationi

Has over 600-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction (By similarity). The main inhibitory site appears to be Thr-73.By similarity1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8TAE6.
MaxQBiQ8TAE6.
PaxDbiQ8TAE6.
PeptideAtlasiQ8TAE6.
PRIDEiQ8TAE6.

PTM databases

iPTMnetiQ8TAE6.
PhosphoSiteiQ8TAE6.

Expressioni

Tissue specificityi

Detected in breast cancer.1 Publication

Gene expression databases

BgeeiQ8TAE6.
CleanExiHS_PPP1R14C.
GenevisibleiQ8TAE6. HS.

Interactioni

Protein-protein interaction databases

BioGridi123578. 5 interactions.
STRINGi9606.ENSP00000355260.

Structurei

3D structure databases

ProteinModelPortaliQ8TAE6.
SMRiQ8TAE6. Positions 71-156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi51 – 566Poly-Ala
Compositional biasi58 – 647Poly-Gln
Compositional biasi102 – 1054Poly-Glu

Sequence similaritiesi

Belongs to the PP1 inhibitor family.Curated

Phylogenomic databases

eggNOGiENOG410IYUT. Eukaryota.
ENOG4112B8C. LUCA.
GeneTreeiENSGT00390000003608.
HOGENOMiHOG000231993.
HOVERGENiHBG054419.
InParanoidiQ8TAE6.
KOiK17556.
OMAiDCHKPTE.
OrthoDBiEOG7RNK21.
PhylomeDBiQ8TAE6.
TreeFamiTF105546.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiIPR008025. PP1_inhibitor.
[Graphical view]
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiPF05361. PP1_inhibitor. 1 hit.
[Graphical view]
SUPFAMiSSF81790. SSF81790. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TAE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVATGSSET AGGASGGGAR VFFQSPRGGA GGSPGSSSGS GSSREDSAPV
60 70 80 90 100
ATAAAAGQVQ QQQQRRHQQG KVTVKYDRKE LRKRLVLEEW IVEQLGQLYG
110 120 130 140 150
CEEEEMPEVE IDIDDLLDAD SDEERASKLQ EALVDCYKPT EEFIKELLSR
160
IRGMRKLSPP QKKSV
Length:165
Mass (Da):17,843
Last modified:March 21, 2006 - v3
Checksum:i514CB1EC4B15E5BB
GO

Sequence cautioni

The sequence AAG48264.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti10 – 101T → A.2 Publications
Corresponds to variant rs2297672 [ dbSNP | Ensembl ].
VAR_025547

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF308297 mRNA. Translation: AAG48264.1. Different initiation.
AF407165 mRNA. Translation: AAL83506.1.
AF407166 Genomic DNA. Translation: AAL83507.1.
AL355497, AL096708 Genomic DNA. Translation: CAH74129.1.
AL096708, AL355497 Genomic DNA. Translation: CAI19346.1.
BC015773 mRNA. Translation: AAH15773.1.
CCDSiCCDS5226.1.
RefSeqiNP_112211.1. NM_030949.2.
UniGeneiHs.486798.

Genome annotation databases

EnsembliENST00000361131; ENSP00000355260; ENSG00000198729.
GeneIDi81706.
KEGGihsa:81706.
UCSCiuc003qnt.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF308297 mRNA. Translation: AAG48264.1. Different initiation.
AF407165 mRNA. Translation: AAL83506.1.
AF407166 Genomic DNA. Translation: AAL83507.1.
AL355497, AL096708 Genomic DNA. Translation: CAH74129.1.
AL096708, AL355497 Genomic DNA. Translation: CAI19346.1.
BC015773 mRNA. Translation: AAH15773.1.
CCDSiCCDS5226.1.
RefSeqiNP_112211.1. NM_030949.2.
UniGeneiHs.486798.

3D structure databases

ProteinModelPortaliQ8TAE6.
SMRiQ8TAE6. Positions 71-156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123578. 5 interactions.
STRINGi9606.ENSP00000355260.

PTM databases

iPTMnetiQ8TAE6.
PhosphoSiteiQ8TAE6.

Polymorphism and mutation databases

BioMutaiPPP1R14C.
DMDMi90110047.

Proteomic databases

EPDiQ8TAE6.
MaxQBiQ8TAE6.
PaxDbiQ8TAE6.
PeptideAtlasiQ8TAE6.
PRIDEiQ8TAE6.

Protocols and materials databases

DNASUi81706.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361131; ENSP00000355260; ENSG00000198729.
GeneIDi81706.
KEGGihsa:81706.
UCSCiuc003qnt.4. human.

Organism-specific databases

CTDi81706.
GeneCardsiPPP1R14C.
HGNCiHGNC:14952. PPP1R14C.
MIMi613242. gene.
neXtProtiNX_Q8TAE6.
PharmGKBiPA33630.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IYUT. Eukaryota.
ENOG4112B8C. LUCA.
GeneTreeiENSGT00390000003608.
HOGENOMiHOG000231993.
HOVERGENiHBG054419.
InParanoidiQ8TAE6.
KOiK17556.
OMAiDCHKPTE.
OrthoDBiEOG7RNK21.
PhylomeDBiQ8TAE6.
TreeFamiTF105546.

Enzyme and pathway databases

SIGNORiQ8TAE6.

Miscellaneous databases

ChiTaRSiPPP1R14C. human.
GeneWikiiPPP1R14C.
GenomeRNAii81706.
NextBioi72067.
PROiQ8TAE6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TAE6.
CleanExiHS_PPP1R14C.
GenevisibleiQ8TAE6. HS.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiIPR008025. PP1_inhibitor.
[Graphical view]
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiPF05361. PP1_inhibitor. 1 hit.
[Graphical view]
SUPFAMiSSF81790. SSF81790. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression."
    Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O., Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J.
    Cancer Immun. 1:4-4(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT ALA-10.
    Tissue: Mammary gland.
  2. "KEPI, a PKC-dependent protein phosphatase 1 inhibitor regulated by morphine."
    Liu Q.-R., Zhang P.-W., Zhen Q., Walther D., Wang X.-B., Uhl G.
    J. Biol. Chem. 277:13312-13320(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-10.
    Tissue: Skin.
  5. "Phosphorylation of protein phosphatase type-1 inhibitory proteins by integrin-linked kinase and cyclic nucleotide-dependent protein kinases."
    Erdodi F., Kiss E., Walsh M.P., Stefansson B., Deng J.T., Eto M., Brautigan D.L., Hartshorne D.J.
    Biochem. Biophys. Res. Commun. 306:382-387(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-73.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPP14C_HUMAN
AccessioniPrimary (citable) accession number: Q8TAE6
Secondary accession number(s): Q5VY83, Q96BB1, Q9H277
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: March 21, 2006
Last modified: May 11, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.