Q8TAC9 (SCAM5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Secretory carrier-associated membrane protein 5
Short name=Secretory carrier membrane protein 5
Short name=hSCAMP5

Gene names

Name:

SCAMP5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway. Ref.1 Ref.7
Subunit structure	Interacts (via C-terminal part) with SYT1 and SYT2; interaction with synaptotagmins making a link with the SNARE molecules. Interacts with SLC9A7. Ref.1 Ref.6
Subcellular location	Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatus › trans-Golgi network membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Multi-pass membrane protein By similarity. Note: Mainly localizes in Golgi apparatus membrane. Upon calcium-triggered exocytosis, it translocates to the cell membrane. Highly enriched in synaptic vesicles By similarity. Ref.1 Ref.6
Tissue specificity	Expressed both by neuronal and nonneuronal tissues. Expressed in brain, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and in the different parts of brain. In thyroid tissues, it is expressed by the follicular epithelial cells. In the adrenal gland tissues it is detected in the zona fasciculate of the cortex region (at protein level). Ref.1
Induction	By endoplasmic reticulum stress. Ref.7
Sequence similarities	Belongs to the SCAMP family. SCAMP5 subfamily.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Cellular component	Cell junction Cell membrane Cytoplasmic vesicle Endosome Golgi apparatus Membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	exocytosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of endocytosis Inferred from direct assay Ref.7. Source: UniProtKB positive regulation of calcium ion-dependent exocytosis Inferred from direct assay Ref.1. Source: UniProtKB positive regulation of cytokine secretion Inferred from direct assay Ref.1. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW response to endoplasmic reticulum stress Inferred from direct assay Ref.7. Source: UniProtKB
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from direct assay Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay Ref.1. Source: UniProtKB recycling endosome membrane Inferred from direct assay Ref.6. Source: UniProtKB synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell trans-Golgi network membrane Inferred from direct assay Ref.6. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.6Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Syt1	P46096	2	EBI-2695784,EBI-445340	From a different organism.
Syt2	P46097	2	EBI-2695784,EBI-457969	From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TAC9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TAC9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     132-132: C → CPTLASSCS

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8TAC9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-98: MAEKVNNFPP...WFRPIYKAFK → MFLPRLRGRYSSPACQHDQAPLLPLDV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Secretory carrier-associated membrane protein 5		PRO_0000191262
Regions
	Topological domain	1 – 39	39	Cytoplasmic Potential
	Transmembrane	40 – 60	21	Helical; Potential
	Topological domain	61 – 67	7	Extracellular Potential
	Transmembrane	68 – 88	21	Helical; Potential
	Topological domain	89 – 102	14	Cytoplasmic Potential
	Transmembrane	103 – 125	23	Helical; Potential
	Topological domain	126 – 148	23	Extracellular Potential
	Transmembrane	149 – 169	21	Helical; Potential
	Topological domain	170 – 235	66	Cytoplasmic Potential
Natural variations
	Alternative sequence	1 – 98	98	MAEKV…YKAFK → MFLPRLRGRYSSPACQHDQA PLLPLDV in isoform 3.		VSP_036934
	Alternative sequence	132	1	C → CPTLASSCS in isoform 2.		VSP_010206

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D3CFF3C9EF2FC6FF	FASTA	235	26,104
	10 20 30 40 50 60 MAEKVNNFPP LPKFIPLKPC FYQDFEADIP PQHVSMTKRL YYLWMLNSVT LAVNLVGCLA 70 80 90 100 110 120 WLIGGGGATN FGLAFLWLIL FTPCSYVCWF RPIYKAFKTD SSFSFMAFFF TFMAQLVISI 130 140 150 160 170 180 IQAVGIPGWG VCGWIATISF FGTNIGSAVV MLIPTVMFTV MAVFSFIALS MVHKFYRGSG 190 200 210 220 230 GSFSKAQEEW TTGAWKNPHV QQAAQNAAMG AAQGAMNQPQ TQYSATPNYT YSNEM
	Isoform 2 [UniParc]. Checksum: A97414ADB7E732B3	FASTA	243	26,851
	10 20 30 40 50 60 MAEKVNNFPP LPKFIPLKPC FYQDFEADIP PQHVSMTKRL YYLWMLNSVT LAVNLVGCLA 70 80 90 100 110 120 WLIGGGGATN FGLAFLWLIL FTPCSYVCWF RPIYKAFKTD SSFSFMAFFF TFMAQLVISI 130 140 150 160 170 180 IQAVGIPGWG VCPTLASSCS GWIATISFFG TNIGSAVVML IPTVMFTVMA VFSFIALSMV 190 200 210 220 230 240 HKFYRGSGGS FSKAQEEWTT GAWKNPHVQQ AAQNAAMGAA QGAMNQPQTQ YSATPNYTYS NEM
	Isoform 3 [UniParc]. Checksum: 2DD71AD52AA4C700	FASTA	164	17,932
	10 20 30 40 50 60 MFLPRLRGRY SSPACQHDQA PLLPLDVTDS SFSFMAFFFT FMAQLVISII QAVGIPGWGV 70 80 90 100 110 120 CGWIATISFF GTNIGSAVVM LIPTVMFTVM AVFSFIALSM VHKFYRGSGG SFSKAQEEWT 130 140 150 160 TGAWKNPHVQ QAAQNAAMGA AQGAMNQPQT QYSATPNYTY SNEM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machinery." Han C., Chen T., Yang M., Li N., Liu H., Cao X. J. Immunol. 182:2986-2996(2009) [PubMed: 19234194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SYT1 AND SYT2.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Brain, Synovial cell, Teratocarcinoma and Thalamus.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Amygdala.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[6]	"Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7." Lin P.J., Williams W.P., Luu Y., Molday R.S., Orlowski J., Numata M. J. Cell Sci. 118:1885-1897(2005) [PubMed: 15840657] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SLC9A7.
[7]	"SCAMP5 links endoplasmic reticulum stress to the accumulation of expanded polyglutamine protein aggregates via endocytosis inhibition." Noh J.-Y., Lee H., Song S., Kim N.S., Im W., Kim M., Seo H.-M., Chung C.-W., Chang J.-W., Ferrante R.J., Yoo Y.-J., Ryu H., Jung Y.-K. J. Biol. Chem. 284:11318-11325(2009) [PubMed: 19240033] [Abstract] Cited for: POSSIBLE FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF495715 mRNA. Translation: AAM18052.1. AK056438 mRNA. Translation: BAG51709.1. AK126940 mRNA. Translation: BAG54404.1. AK127480 mRNA. Translation: BAG54511.1. AK301505 mRNA. Translation: BAH13498.1. AL834226 mRNA. Translation: CAD38904.1. CH471136 Genomic DNA. Translation: EAW99277.1. CH471136 Genomic DNA. Translation: EAW99278.1. CH471136 Genomic DNA. Translation: EAW99280.1. CH471136 Genomic DNA. Translation: EAW99283.1. BC024700 mRNA. Translation: AAH24700.1.
IPI	IPI00395747. IPI00478460. IPI00922319.
RefSeq	NP_001171582.1. NM_001178111.1. NP_001171583.1. NM_001178112.1. NP_620417.1. NM_138967.3.
UniGene	Hs.374180.
3D structure databases
ProteinModelPortal	Q8TAC9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TAC9. 8 interactions.
STRING	Q8TAC9.
Polymorphism databases
DMDM	47117253.
Proteomic databases
PRIDE	Q8TAC9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000425597; ENSP00000406547; ENSG00000198794.
GeneID	192683.
KEGG	hsa:192683.
UCSC	uc002azk.1. human. uc002azn.1. human.
Organism-specific databases
CTD	192683.
GeneCards	GC15P075287.
H-InvDB	HIX0012435.
HGNC	HGNC:30386. SCAMP5.
MIM	613766. gene.
neXtProt	NX_Q8TAC9.
PharmGKB	PA134962580.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG05427.
GeneTree	ENSGT00390000014393.
HOVERGEN	HBG071938.
InParanoid	Q8TAC9.
OMA	SFFGTNI.
OrthoDB	EOG451DRT.
PhylomeDB	Q8TAC9.
Gene expression databases
ArrayExpress	Q8TAC9.
Bgee	Q8TAC9.
CleanEx	HS_SCAMP5.
Genevestigator	Q8TAC9.
GermOnline	ENSG00000198794. Homo sapiens.
Family and domain databases
InterPro	IPR007273. SCAMP. [Graphical view]
PANTHER	PTHR10687. SCAMP. 1 hit.
Pfam	PF04144. SCAMP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	89383.
SOURCE	Search...

Entry information

Entry name

SCAM5_HUMAN

Accession

Primary (citable) accession number: Q8TAC9
Secondary accession number(s): B3KPJ7 , B7Z762, D3DW71, Q8N3M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.