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Q8TAC9

- SCAM5_HUMAN

UniProt

Q8TAC9 - SCAM5_HUMAN

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Protein

Secretory carrier-associated membrane protein 5

Gene

SCAMP5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.1 Publication

GO - Biological processi

  1. exocytosis Source: UniProtKB-KW
  2. negative regulation of endocytosis Source: UniProtKB
  3. positive regulation of calcium ion-dependent exocytosis Source: UniProtKB
  4. positive regulation of cytokine secretion Source: UniProtKB
  5. protein transport Source: UniProtKB-KW
  6. response to endoplasmic reticulum stress Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Secretory carrier-associated membrane protein 5
Short name:
Secretory carrier membrane protein 5
Short name:
hSCAMP5
Gene namesi
Name:SCAMP5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:30386. SCAMP5.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatustrans-Golgi network membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane By similarity; Multi-pass membrane protein By similarity
Note: Mainly localizes in Golgi apparatus membrane. Upon calcium-triggered exocytosis, it translocates to the cell membrane. Highly enriched in synaptic vesicles (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3939CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei40 – 6021HelicalSequence AnalysisAdd
BLAST
Topological domaini61 – 677ExtracellularSequence Analysis
Transmembranei68 – 8821HelicalSequence AnalysisAdd
BLAST
Topological domaini89 – 10214CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei103 – 12523HelicalSequence AnalysisAdd
BLAST
Topological domaini126 – 14823ExtracellularSequence AnalysisAdd
BLAST
Transmembranei149 – 16921HelicalSequence AnalysisAdd
BLAST
Topological domaini170 – 23566CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cytoplasmic vesicle Source: UniProtKB-KW
  3. Golgi membrane Source: UniProtKB
  4. integral component of membrane Source: UniProtKB
  5. plasma membrane Source: UniProtKB
  6. recycling endosome membrane Source: UniProtKB
  7. synapse Source: UniProtKB-KW
  8. trans-Golgi network membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134962580.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 235235Secretory carrier-associated membrane protein 5PRO_0000191262Add
BLAST

Proteomic databases

MaxQBiQ8TAC9.
PaxDbiQ8TAC9.
PRIDEiQ8TAC9.

PTM databases

PhosphoSiteiQ8TAC9.

Expressioni

Tissue specificityi

Expressed both by neuronal and non-neuronal tissues. Expressed in brain, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and in the different parts of brain. In thyroid tissues, it is expressed by the follicular epithelial cells. In the adrenal gland tissues it is detected in the zona fasciculata of the cortex region (at protein level).1 Publication

Inductioni

By endoplasmic reticulum stress.1 Publication

Gene expression databases

BgeeiQ8TAC9.
CleanExiHS_SCAMP5.
ExpressionAtlasiQ8TAC9. baseline and differential.
GenevestigatoriQ8TAC9.

Organism-specific databases

HPAiHPA046645.

Interactioni

Subunit structurei

Interacts (via C-terminal part) with SYT1 and SYT2; interaction with synaptotagmins making a link with the SNARE molecules. Interacts with SLC9A7.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Syt1P460962EBI-2695784,EBI-445340From a different organism.
Syt2P460972EBI-2695784,EBI-457969From a different organism.

Protein-protein interaction databases

BioGridi128182. 3 interactions.
IntActiQ8TAC9. 9 interactions.
MINTiMINT-4723804.
STRINGi9606.ENSP00000406547.

Structurei

3D structure databases

ProteinModelPortaliQ8TAC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SCAMP family. SCAMP5 subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG114663.
GeneTreeiENSGT00390000014393.
HOGENOMiHOG000294221.
HOVERGENiHBG071938.
InParanoidiQ8TAC9.
OMAiNSITLAM.
PhylomeDBiQ8TAC9.
TreeFamiTF313797.

Family and domain databases

InterProiIPR007273. SCAMP.
[Graphical view]
PANTHERiPTHR10687. PTHR10687. 1 hit.
PfamiPF04144. SCAMP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8TAC9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEKVNNFPP LPKFIPLKPC FYQDFEADIP PQHVSMTKRL YYLWMLNSVT
60 70 80 90 100
LAVNLVGCLA WLIGGGGATN FGLAFLWLIL FTPCSYVCWF RPIYKAFKTD
110 120 130 140 150
SSFSFMAFFF TFMAQLVISI IQAVGIPGWG VCGWIATISF FGTNIGSAVV
160 170 180 190 200
MLIPTVMFTV MAVFSFIALS MVHKFYRGSG GSFSKAQEEW TTGAWKNPHV
210 220 230
QQAAQNAAMG AAQGAMNQPQ TQYSATPNYT YSNEM
Length:235
Mass (Da):26,104
Last modified:June 1, 2002 - v1
Checksum:iD3CFF3C9EF2FC6FF
GO
Isoform 2 (identifier: Q8TAC9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     132-132: C → CPTLASSCS

Note: No experimental confirmation available.

Show »
Length:243
Mass (Da):26,851
Checksum:iA97414ADB7E732B3
GO
Isoform 3 (identifier: Q8TAC9-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-98: MAEKVNNFPP...WFRPIYKAFK → MFLPRLRGRYSSPACQHDQAPLLPLDV

Note: No experimental confirmation available.

Show »
Length:164
Mass (Da):17,932
Checksum:i2DD71AD52AA4C700
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9898MAEKV…YKAFK → MFLPRLRGRYSSPACQHDQA PLLPLDV in isoform 3. 1 PublicationVSP_036934Add
BLAST
Alternative sequencei132 – 1321C → CPTLASSCS in isoform 2. 1 PublicationVSP_010206

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF495715 mRNA. Translation: AAM18052.1.
AK056438 mRNA. Translation: BAG51709.1.
AK126940 mRNA. Translation: BAG54404.1.
AK127480 mRNA. Translation: BAG54511.1.
AK301505 mRNA. Translation: BAH13498.1.
AL834226 mRNA. Translation: CAD38904.1.
CH471136 Genomic DNA. Translation: EAW99277.1.
CH471136 Genomic DNA. Translation: EAW99278.1.
CH471136 Genomic DNA. Translation: EAW99280.1.
CH471136 Genomic DNA. Translation: EAW99283.1.
BC024700 mRNA. Translation: AAH24700.1.
CCDSiCCDS45306.1. [Q8TAC9-1]
RefSeqiNP_001171582.1. NM_001178111.1. [Q8TAC9-1]
NP_001171583.1. NM_001178112.1. [Q8TAC9-1]
NP_620417.1. NM_138967.3. [Q8TAC9-1]
XP_006720484.1. XM_006720421.1. [Q8TAC9-1]
XP_006720485.1. XM_006720422.1. [Q8TAC9-3]
UniGeneiHs.374180.

Genome annotation databases

EnsembliENST00000361900; ENSP00000355387; ENSG00000198794. [Q8TAC9-1]
ENST00000425597; ENSP00000406547; ENSG00000198794. [Q8TAC9-1]
ENST00000562212; ENSP00000455313; ENSG00000198794. [Q8TAC9-2]
GeneIDi192683.
KEGGihsa:192683.
UCSCiuc002azk.2. human. [Q8TAC9-1]
uc002azn.2. human. [Q8TAC9-2]
uc010uly.2. human. [Q8TAC9-3]

Polymorphism databases

DMDMi47117253.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF495715 mRNA. Translation: AAM18052.1 .
AK056438 mRNA. Translation: BAG51709.1 .
AK126940 mRNA. Translation: BAG54404.1 .
AK127480 mRNA. Translation: BAG54511.1 .
AK301505 mRNA. Translation: BAH13498.1 .
AL834226 mRNA. Translation: CAD38904.1 .
CH471136 Genomic DNA. Translation: EAW99277.1 .
CH471136 Genomic DNA. Translation: EAW99278.1 .
CH471136 Genomic DNA. Translation: EAW99280.1 .
CH471136 Genomic DNA. Translation: EAW99283.1 .
BC024700 mRNA. Translation: AAH24700.1 .
CCDSi CCDS45306.1. [Q8TAC9-1 ]
RefSeqi NP_001171582.1. NM_001178111.1. [Q8TAC9-1 ]
NP_001171583.1. NM_001178112.1. [Q8TAC9-1 ]
NP_620417.1. NM_138967.3. [Q8TAC9-1 ]
XP_006720484.1. XM_006720421.1. [Q8TAC9-1 ]
XP_006720485.1. XM_006720422.1. [Q8TAC9-3 ]
UniGenei Hs.374180.

3D structure databases

ProteinModelPortali Q8TAC9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 128182. 3 interactions.
IntActi Q8TAC9. 9 interactions.
MINTi MINT-4723804.
STRINGi 9606.ENSP00000406547.

PTM databases

PhosphoSitei Q8TAC9.

Polymorphism databases

DMDMi 47117253.

Proteomic databases

MaxQBi Q8TAC9.
PaxDbi Q8TAC9.
PRIDEi Q8TAC9.

Protocols and materials databases

DNASUi 192683.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000361900 ; ENSP00000355387 ; ENSG00000198794 . [Q8TAC9-1 ]
ENST00000425597 ; ENSP00000406547 ; ENSG00000198794 . [Q8TAC9-1 ]
ENST00000562212 ; ENSP00000455313 ; ENSG00000198794 . [Q8TAC9-2 ]
GeneIDi 192683.
KEGGi hsa:192683.
UCSCi uc002azk.2. human. [Q8TAC9-1 ]
uc002azn.2. human. [Q8TAC9-2 ]
uc010uly.2. human. [Q8TAC9-3 ]

Organism-specific databases

CTDi 192683.
GeneCardsi GC15P075287.
HGNCi HGNC:30386. SCAMP5.
HPAi HPA046645.
MIMi 613766. gene.
neXtProti NX_Q8TAC9.
PharmGKBi PA134962580.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG114663.
GeneTreei ENSGT00390000014393.
HOGENOMi HOG000294221.
HOVERGENi HBG071938.
InParanoidi Q8TAC9.
OMAi NSITLAM.
PhylomeDBi Q8TAC9.
TreeFami TF313797.

Miscellaneous databases

ChiTaRSi SCAMP5. human.
GeneWikii SCAMP5.
GenomeRNAii 192683.
NextBioi 89383.
PROi Q8TAC9.
SOURCEi Search...

Gene expression databases

Bgeei Q8TAC9.
CleanExi HS_SCAMP5.
ExpressionAtlasi Q8TAC9. baseline and differential.
Genevestigatori Q8TAC9.

Family and domain databases

InterProi IPR007273. SCAMP.
[Graphical view ]
PANTHERi PTHR10687. PTHR10687. 1 hit.
Pfami PF04144. SCAMP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machinery."
    Han C., Chen T., Yang M., Li N., Liu H., Cao X.
    J. Immunol. 182:2986-2996(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SYT1 AND SYT2.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Brain, Synovial cell, Teratocarcinoma and Thalamus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Amygdala.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  6. "Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7."
    Lin P.J., Williams W.P., Luu Y., Molday R.S., Orlowski J., Numata M.
    J. Cell Sci. 118:1885-1897(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SLC9A7.
  7. "SCAMP5 links endoplasmic reticulum stress to the accumulation of expanded polyglutamine protein aggregates via endocytosis inhibition."
    Noh J.-Y., Lee H., Song S., Kim N.S., Im W., Kim M., Seo H.-M., Chung C.-W., Chang J.-W., Ferrante R.J., Yoo Y.-J., Ryu H., Jung Y.-K.
    J. Biol. Chem. 284:11318-11325(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION, INDUCTION.

Entry informationi

Entry nameiSCAM5_HUMAN
AccessioniPrimary (citable) accession number: Q8TAC9
Secondary accession number(s): B3KPJ7
, B7Z762, D3DW71, Q8N3M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3