Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8TAC9 (SCAM5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Secretory carrier-associated membrane protein 5

Short name=Secretory carrier membrane protein 5
Short name=hSCAMP5
Gene names
Name:SCAMP5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length235 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway. Ref.1 Ref.7

Subunit structure

Interacts (via C-terminal part) with SYT1 and SYT2; interaction with synaptotagmins making a link with the SNARE molecules. Interacts with SLC9A7. Ref.1 Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatustrans-Golgi network membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Multi-pass membrane protein By similarity. Note: Mainly localizes in Golgi apparatus membrane. Upon calcium-triggered exocytosis, it translocates to the cell membrane. Highly enriched in synaptic vesicles By similarity. Ref.1 Ref.6

Tissue specificity

Expressed both by neuronal and non-neuronal tissues. Expressed in brain, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and in the different parts of brain. In thyroid tissues, it is expressed by the follicular epithelial cells. In the adrenal gland tissues it is detected in the zona fasciculata of the cortex region (at protein level). Ref.1

Induction

By endoplasmic reticulum stress. Ref.7

Sequence similarities

Belongs to the SCAMP family. SCAMP5 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Syt1P460962EBI-2695784,EBI-445340From a different organism.
Syt2P460972EBI-2695784,EBI-457969From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TAC9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8TAC9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     132-132: C → CPTLASSCS
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8TAC9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-98: MAEKVNNFPP...WFRPIYKAFK → MFLPRLRGRYSSPACQHDQAPLLPLDV
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 235235Secretory carrier-associated membrane protein 5
PRO_0000191262

Regions

Topological domain1 – 3939Cytoplasmic Potential
Transmembrane40 – 6021Helical; Potential
Topological domain61 – 677Extracellular Potential
Transmembrane68 – 8821Helical; Potential
Topological domain89 – 10214Cytoplasmic Potential
Transmembrane103 – 12523Helical; Potential
Topological domain126 – 14823Extracellular Potential
Transmembrane149 – 16921Helical; Potential
Topological domain170 – 23566Cytoplasmic Potential

Natural variations

Alternative sequence1 – 9898MAEKV…YKAFK → MFLPRLRGRYSSPACQHDQA PLLPLDV in isoform 3.
VSP_036934
Alternative sequence1321C → CPTLASSCS in isoform 2.
VSP_010206

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: D3CFF3C9EF2FC6FF

FASTA23526,104
        10         20         30         40         50         60 
MAEKVNNFPP LPKFIPLKPC FYQDFEADIP PQHVSMTKRL YYLWMLNSVT LAVNLVGCLA 

        70         80         90        100        110        120 
WLIGGGGATN FGLAFLWLIL FTPCSYVCWF RPIYKAFKTD SSFSFMAFFF TFMAQLVISI 

       130        140        150        160        170        180 
IQAVGIPGWG VCGWIATISF FGTNIGSAVV MLIPTVMFTV MAVFSFIALS MVHKFYRGSG 

       190        200        210        220        230 
GSFSKAQEEW TTGAWKNPHV QQAAQNAAMG AAQGAMNQPQ TQYSATPNYT YSNEM 

« Hide

Isoform 2 [UniParc].

Checksum: A97414ADB7E732B3
Show »

FASTA24326,851
Isoform 3 [UniParc].

Checksum: 2DD71AD52AA4C700
Show »

FASTA16417,932

References

« Hide 'large scale' references
[1]"Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machinery."
Han C., Chen T., Yang M., Li N., Liu H., Cao X.
J. Immunol. 182:2986-2996(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SYT1 AND SYT2.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Brain, Synovial cell, Teratocarcinoma and Thalamus.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Amygdala.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[6]"Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7."
Lin P.J., Williams W.P., Luu Y., Molday R.S., Orlowski J., Numata M.
J. Cell Sci. 118:1885-1897(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SLC9A7.
[7]"SCAMP5 links endoplasmic reticulum stress to the accumulation of expanded polyglutamine protein aggregates via endocytosis inhibition."
Noh J.-Y., Lee H., Song S., Kim N.S., Im W., Kim M., Seo H.-M., Chung C.-W., Chang J.-W., Ferrante R.J., Yoo Y.-J., Ryu H., Jung Y.-K.
J. Biol. Chem. 284:11318-11325(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: POSSIBLE FUNCTION, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF495715 mRNA. Translation: AAM18052.1.
AK056438 mRNA. Translation: BAG51709.1.
AK126940 mRNA. Translation: BAG54404.1.
AK127480 mRNA. Translation: BAG54511.1.
AK301505 mRNA. Translation: BAH13498.1.
AL834226 mRNA. Translation: CAD38904.1.
CH471136 Genomic DNA. Translation: EAW99277.1.
CH471136 Genomic DNA. Translation: EAW99278.1.
CH471136 Genomic DNA. Translation: EAW99280.1.
CH471136 Genomic DNA. Translation: EAW99283.1.
BC024700 mRNA. Translation: AAH24700.1.
RefSeqNP_001171582.1. NM_001178111.1.
NP_001171583.1. NM_001178112.1.
NP_620417.1. NM_138967.3.
UniGeneHs.374180.

3D structure databases

ProteinModelPortalQ8TAC9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid128182. 2 interactions.
IntActQ8TAC9. 9 interactions.
MINTMINT-4723804.
STRING9606.ENSP00000406547.

PTM databases

PhosphoSiteQ8TAC9.

Polymorphism databases

DMDM47117253.

Proteomic databases

PaxDbQ8TAC9.
PRIDEQ8TAC9.

Protocols and materials databases

DNASU192683.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361900; ENSP00000355387; ENSG00000198794. [Q8TAC9-1]
ENST00000425597; ENSP00000406547; ENSG00000198794. [Q8TAC9-1]
ENST00000545456; ENSP00000439685; ENSG00000198794. [Q8TAC9-3]
ENST00000562212; ENSP00000455313; ENSG00000198794. [Q8TAC9-2]
GeneID192683.
KEGGhsa:192683.
UCSCuc002azk.2. human. [Q8TAC9-1]
uc002azn.2. human. [Q8TAC9-2]
uc010uly.2. human. [Q8TAC9-3]

Organism-specific databases

CTD192683.
GeneCardsGC15P075287.
HGNCHGNC:30386. SCAMP5.
HPAHPA046645.
MIM613766. gene.
neXtProtNX_Q8TAC9.
PharmGKBPA134962580.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG114663.
HOGENOMHOG000294221.
HOVERGENHBG071938.
InParanoidQ8TAC9.
OMANSITLAM.
PhylomeDBQ8TAC9.
TreeFamTF313797.

Gene expression databases

ArrayExpressQ8TAC9.
BgeeQ8TAC9.
CleanExHS_SCAMP5.
GenevestigatorQ8TAC9.

Family and domain databases

InterProIPR007273. SCAMP.
[Graphical view]
PANTHERPTHR10687. PTHR10687. 1 hit.
PfamPF04144. SCAMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSCAMP5.
GenomeRNAi192683.
NextBio89383.
PROQ8TAC9.
SOURCESearch...

Entry information

Entry nameSCAM5_HUMAN
AccessionPrimary (citable) accession number: Q8TAC9
Secondary accession number(s): B3KPJ7 expand/collapse secondary AC list , B7Z762, D3DW71, Q8N3M4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2002
Last modified: March 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM