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Q8TAC9

- SCAM5_HUMAN

UniProt

Q8TAC9 - SCAM5_HUMAN

Protein

Secretory carrier-associated membrane protein 5

Gene

SCAMP5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. exocytosis Source: UniProtKB-KW
    2. negative regulation of endocytosis Source: UniProtKB
    3. positive regulation of calcium ion-dependent exocytosis Source: UniProtKB
    4. positive regulation of cytokine secretion Source: UniProtKB
    5. protein transport Source: UniProtKB-KW
    6. response to endoplasmic reticulum stress Source: UniProtKB

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Secretory carrier-associated membrane protein 5
    Short name:
    Secretory carrier membrane protein 5
    Short name:
    hSCAMP5
    Gene namesi
    Name:SCAMP5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:30386. SCAMP5.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatustrans-Golgi network membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane By similarity; Multi-pass membrane protein By similarity
    Note: Mainly localizes in Golgi apparatus membrane. Upon calcium-triggered exocytosis, it translocates to the cell membrane. Highly enriched in synaptic vesicles By similarity.By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. Golgi membrane Source: UniProtKB
    3. integral component of membrane Source: UniProtKB
    4. plasma membrane Source: UniProtKB
    5. recycling endosome membrane Source: UniProtKB
    6. synaptic vesicle membrane Source: UniProtKB-SubCell
    7. trans-Golgi network membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane, Synapse

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134962580.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 235235Secretory carrier-associated membrane protein 5PRO_0000191262Add
    BLAST

    Proteomic databases

    MaxQBiQ8TAC9.
    PaxDbiQ8TAC9.
    PRIDEiQ8TAC9.

    PTM databases

    PhosphoSiteiQ8TAC9.

    Expressioni

    Tissue specificityi

    Expressed both by neuronal and non-neuronal tissues. Expressed in brain, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and in the different parts of brain. In thyroid tissues, it is expressed by the follicular epithelial cells. In the adrenal gland tissues it is detected in the zona fasciculata of the cortex region (at protein level).1 Publication

    Inductioni

    By endoplasmic reticulum stress.1 Publication

    Gene expression databases

    ArrayExpressiQ8TAC9.
    BgeeiQ8TAC9.
    CleanExiHS_SCAMP5.
    GenevestigatoriQ8TAC9.

    Organism-specific databases

    HPAiHPA046645.

    Interactioni

    Subunit structurei

    Interacts (via C-terminal part) with SYT1 and SYT2; interaction with synaptotagmins making a link with the SNARE molecules. Interacts with SLC9A7.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Syt1P460962EBI-2695784,EBI-445340From a different organism.
    Syt2P460972EBI-2695784,EBI-457969From a different organism.

    Protein-protein interaction databases

    BioGridi128182. 2 interactions.
    IntActiQ8TAC9. 9 interactions.
    MINTiMINT-4723804.
    STRINGi9606.ENSP00000406547.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TAC9.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3939CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini61 – 677ExtracellularSequence Analysis
    Topological domaini89 – 10214CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini126 – 14823ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini170 – 23566CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei40 – 6021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei68 – 8821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei103 – 12523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei149 – 16921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SCAMP family. SCAMP5 subfamily.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG114663.
    HOGENOMiHOG000294221.
    HOVERGENiHBG071938.
    InParanoidiQ8TAC9.
    OMAiNSITLAM.
    PhylomeDBiQ8TAC9.
    TreeFamiTF313797.

    Family and domain databases

    InterProiIPR007273. SCAMP.
    [Graphical view]
    PANTHERiPTHR10687. PTHR10687. 1 hit.
    PfamiPF04144. SCAMP. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8TAC9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEKVNNFPP LPKFIPLKPC FYQDFEADIP PQHVSMTKRL YYLWMLNSVT    50
    LAVNLVGCLA WLIGGGGATN FGLAFLWLIL FTPCSYVCWF RPIYKAFKTD 100
    SSFSFMAFFF TFMAQLVISI IQAVGIPGWG VCGWIATISF FGTNIGSAVV 150
    MLIPTVMFTV MAVFSFIALS MVHKFYRGSG GSFSKAQEEW TTGAWKNPHV 200
    QQAAQNAAMG AAQGAMNQPQ TQYSATPNYT YSNEM 235
    Length:235
    Mass (Da):26,104
    Last modified:June 1, 2002 - v1
    Checksum:iD3CFF3C9EF2FC6FF
    GO
    Isoform 2 (identifier: Q8TAC9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         132-132: C → CPTLASSCS

    Note: No experimental confirmation available.

    Show »
    Length:243
    Mass (Da):26,851
    Checksum:iA97414ADB7E732B3
    GO
    Isoform 3 (identifier: Q8TAC9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-98: MAEKVNNFPP...WFRPIYKAFK → MFLPRLRGRYSSPACQHDQAPLLPLDV

    Note: No experimental confirmation available.

    Show »
    Length:164
    Mass (Da):17,932
    Checksum:i2DD71AD52AA4C700
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9898MAEKV…YKAFK → MFLPRLRGRYSSPACQHDQA PLLPLDV in isoform 3. 1 PublicationVSP_036934Add
    BLAST
    Alternative sequencei132 – 1321C → CPTLASSCS in isoform 2. 1 PublicationVSP_010206

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF495715 mRNA. Translation: AAM18052.1.
    AK056438 mRNA. Translation: BAG51709.1.
    AK126940 mRNA. Translation: BAG54404.1.
    AK127480 mRNA. Translation: BAG54511.1.
    AK301505 mRNA. Translation: BAH13498.1.
    AL834226 mRNA. Translation: CAD38904.1.
    CH471136 Genomic DNA. Translation: EAW99277.1.
    CH471136 Genomic DNA. Translation: EAW99278.1.
    CH471136 Genomic DNA. Translation: EAW99280.1.
    CH471136 Genomic DNA. Translation: EAW99283.1.
    BC024700 mRNA. Translation: AAH24700.1.
    CCDSiCCDS45306.1. [Q8TAC9-1]
    RefSeqiNP_001171582.1. NM_001178111.1. [Q8TAC9-1]
    NP_001171583.1. NM_001178112.1. [Q8TAC9-1]
    NP_620417.1. NM_138967.3. [Q8TAC9-1]
    XP_006720484.1. XM_006720421.1. [Q8TAC9-1]
    XP_006720485.1. XM_006720422.1. [Q8TAC9-3]
    UniGeneiHs.374180.

    Genome annotation databases

    EnsembliENST00000361900; ENSP00000355387; ENSG00000198794. [Q8TAC9-1]
    ENST00000425597; ENSP00000406547; ENSG00000198794. [Q8TAC9-1]
    ENST00000562212; ENSP00000455313; ENSG00000198794. [Q8TAC9-2]
    GeneIDi192683.
    KEGGihsa:192683.
    UCSCiuc002azk.2. human. [Q8TAC9-1]
    uc002azn.2. human. [Q8TAC9-2]
    uc010uly.2. human. [Q8TAC9-3]

    Polymorphism databases

    DMDMi47117253.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF495715 mRNA. Translation: AAM18052.1 .
    AK056438 mRNA. Translation: BAG51709.1 .
    AK126940 mRNA. Translation: BAG54404.1 .
    AK127480 mRNA. Translation: BAG54511.1 .
    AK301505 mRNA. Translation: BAH13498.1 .
    AL834226 mRNA. Translation: CAD38904.1 .
    CH471136 Genomic DNA. Translation: EAW99277.1 .
    CH471136 Genomic DNA. Translation: EAW99278.1 .
    CH471136 Genomic DNA. Translation: EAW99280.1 .
    CH471136 Genomic DNA. Translation: EAW99283.1 .
    BC024700 mRNA. Translation: AAH24700.1 .
    CCDSi CCDS45306.1. [Q8TAC9-1 ]
    RefSeqi NP_001171582.1. NM_001178111.1. [Q8TAC9-1 ]
    NP_001171583.1. NM_001178112.1. [Q8TAC9-1 ]
    NP_620417.1. NM_138967.3. [Q8TAC9-1 ]
    XP_006720484.1. XM_006720421.1. [Q8TAC9-1 ]
    XP_006720485.1. XM_006720422.1. [Q8TAC9-3 ]
    UniGenei Hs.374180.

    3D structure databases

    ProteinModelPortali Q8TAC9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 128182. 2 interactions.
    IntActi Q8TAC9. 9 interactions.
    MINTi MINT-4723804.
    STRINGi 9606.ENSP00000406547.

    PTM databases

    PhosphoSitei Q8TAC9.

    Polymorphism databases

    DMDMi 47117253.

    Proteomic databases

    MaxQBi Q8TAC9.
    PaxDbi Q8TAC9.
    PRIDEi Q8TAC9.

    Protocols and materials databases

    DNASUi 192683.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361900 ; ENSP00000355387 ; ENSG00000198794 . [Q8TAC9-1 ]
    ENST00000425597 ; ENSP00000406547 ; ENSG00000198794 . [Q8TAC9-1 ]
    ENST00000562212 ; ENSP00000455313 ; ENSG00000198794 . [Q8TAC9-2 ]
    GeneIDi 192683.
    KEGGi hsa:192683.
    UCSCi uc002azk.2. human. [Q8TAC9-1 ]
    uc002azn.2. human. [Q8TAC9-2 ]
    uc010uly.2. human. [Q8TAC9-3 ]

    Organism-specific databases

    CTDi 192683.
    GeneCardsi GC15P075287.
    HGNCi HGNC:30386. SCAMP5.
    HPAi HPA046645.
    MIMi 613766. gene.
    neXtProti NX_Q8TAC9.
    PharmGKBi PA134962580.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG114663.
    HOGENOMi HOG000294221.
    HOVERGENi HBG071938.
    InParanoidi Q8TAC9.
    OMAi NSITLAM.
    PhylomeDBi Q8TAC9.
    TreeFami TF313797.

    Miscellaneous databases

    GeneWikii SCAMP5.
    GenomeRNAii 192683.
    NextBioi 89383.
    PROi Q8TAC9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8TAC9.
    Bgeei Q8TAC9.
    CleanExi HS_SCAMP5.
    Genevestigatori Q8TAC9.

    Family and domain databases

    InterProi IPR007273. SCAMP.
    [Graphical view ]
    PANTHERi PTHR10687. PTHR10687. 1 hit.
    Pfami PF04144. SCAMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machinery."
      Han C., Chen T., Yang M., Li N., Liu H., Cao X.
      J. Immunol. 182:2986-2996(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SYT1 AND SYT2.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Tissue: Brain, Synovial cell, Teratocarcinoma and Thalamus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Amygdala.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    6. "Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7."
      Lin P.J., Williams W.P., Luu Y., Molday R.S., Orlowski J., Numata M.
      J. Cell Sci. 118:1885-1897(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SLC9A7.
    7. "SCAMP5 links endoplasmic reticulum stress to the accumulation of expanded polyglutamine protein aggregates via endocytosis inhibition."
      Noh J.-Y., Lee H., Song S., Kim N.S., Im W., Kim M., Seo H.-M., Chung C.-W., Chang J.-W., Ferrante R.J., Yoo Y.-J., Ryu H., Jung Y.-K.
      J. Biol. Chem. 284:11318-11325(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiSCAM5_HUMAN
    AccessioniPrimary (citable) accession number: Q8TAC9
    Secondary accession number(s): B3KPJ7
    , B7Z762, D3DW71, Q8N3M4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3