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Protein

Josephin-2

Gene

JOSD2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme.3 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei24NucleophilePROSITE-ProRule annotation1
Active sitei125Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • protein deubiquitination Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-5689877. Josephin domain DUBs.

Protein family/group databases

MEROPSiC86.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-2 (EC:3.4.19.12)
Alternative name(s):
Josephin domain-containing protein 2
Gene namesi
Name:JOSD2
ORF Names:SBBI54
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000161677.11.
HGNCiHGNC:28853. JOSD2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24C → A: Loss of deubiquitination activity, no change in subcellular location. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000161677.
PharmGKBiPA142671645.

Polymorphism and mutation databases

DMDMi29840785.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538431 – 188Josephin-2Add BLAST188

Proteomic databases

MaxQBiQ8TAC2.
PaxDbiQ8TAC2.
PeptideAtlasiQ8TAC2.
PRIDEiQ8TAC2.
TopDownProteomicsiQ8TAC2-1. [Q8TAC2-1]

PTM databases

iPTMnetiQ8TAC2.
PhosphoSitePlusiQ8TAC2.

Expressioni

Gene expression databases

BgeeiENSG00000161677.
CleanExiHS_JOSD2.
ExpressionAtlasiQ8TAC2. baseline and differential.
GenevisibleiQ8TAC2. HS.

Interactioni

Protein-protein interaction databases

BioGridi125956. 18 interactors.
IntActiQ8TAC2. 3 interactors.
STRINGi9606.ENSP00000375691.

Structurei

3D structure databases

ProteinModelPortaliQ8TAC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 188JosephinPROSITE-ProRule annotationAdd BLAST178

Phylogenomic databases

eggNOGiKOG2934. Eukaryota.
ENOG4111I4J. LUCA.
GeneTreeiENSGT00390000009228.
HOVERGENiHBG039523.
InParanoidiQ8TAC2.
KOiK15235.
OMAiVEEKGCW.
OrthoDBiEOG091G0NAU.
PhylomeDBiQ8TAC2.
TreeFamiTF313660.

Family and domain databases

InterProiView protein in InterPro
IPR006155. Josephin.
PfamiView protein in Pfam
PF02099. Josephin. 1 hit.
SMARTiView protein in SMART
SM01246. Josephin. 1 hit.
PROSITEiView protein in PROSITE
PS50957. JOSEPHIN. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TAC2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQAPGAQPS PPTVYHERQR LELCAVHALN NVLQQQLFSQ EAADEICKRL
60 70 80 90 100
APDSRLNPHR SLLGTGNYDV NVIMAALQGL GLAAVWWDRR RPLSQLALPQ
110 120 130 140 150
VLGLILNLPS PVSLGLLSLP LRRRHWVALR QVDGVYYNLD SKLRAPEALG
160 170 180
DEDGVRAFLA AALAQGLCEV LLVVTKEVEE KGSWLRTD
Length:188
Mass (Da):20,756
Last modified:June 1, 2002 - v1
Checksum:iA2832B4D9BD675E9
GO
Isoform 2 (identifier: Q8TAC2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     49-90: Missing.

Note: No experimental confirmation available.
Show »
Length:146
Mass (Da):16,100
Checksum:i86386376B0F16FDB
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04753749 – 90Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247787 mRNA. Translation: AAL95692.1.
AK131052 mRNA. No translation available.
AC008743 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW71877.1.
BC062416 mRNA. Translation: AAH62416.1.
CCDSiCCDS12797.1. [Q8TAC2-1]
CCDS59413.1. [Q8TAC2-2]
RefSeqiNP_001257568.1. NM_001270639.1. [Q8TAC2-1]
NP_001257569.1. NM_001270640.1. [Q8TAC2-1]
NP_001257570.1. NM_001270641.1. [Q8TAC2-2]
NP_001257615.1. NM_001270686.1. [Q8TAC2-1]
NP_612207.1. NM_138334.3. [Q8TAC2-1]
XP_011524736.1. XM_011526434.2. [Q8TAC2-1]
UniGeneiHs.467151.
Hs.737597.

Genome annotation databases

EnsembliENST00000595669; ENSP00000468860; ENSG00000161677. [Q8TAC2-2]
ENST00000598418; ENSP00000468956; ENSG00000161677. [Q8TAC2-1]
ENST00000601423; ENSP00000472116; ENSG00000161677. [Q8TAC2-1]
GeneIDi126119.
KEGGihsa:126119.
UCSCiuc002pso.3. human. [Q8TAC2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiJOS2_HUMAN
AccessioniPrimary (citable) accession number: Q8TAC2
Secondary accession number(s): M0QX25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: June 1, 2002
Last modified: September 27, 2017
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot