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Q8TAC2 (JOS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Josephin-2

EC=3.4.19.12
Alternative name(s):
Josephin domain-containing protein 2
Gene names
Name:JOSD2
ORF Names:SBBI54
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme. Ref.6 Ref.7 Ref.8

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.6 Ref.7

Subcellular location

Cytoplasmcytosol Ref.8.

Sequence similarities

Contains 1 Josephin domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
Protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein deubiquitination

Inferred from direct assay Ref.7. Source: UniProtKB

   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionomega peptidase activity

Inferred from electronic annotation. Source: InterPro

ubiquitin-specific protease activity

Inferred from direct assay Ref.7. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TAC2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8TAC2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     49-90: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188Josephin-2
PRO_0000053843

Regions

Domain11 – 188178Josephin

Sites

Active site241Nucleophile By similarity
Active site1251Proton acceptor By similarity

Natural variations

Alternative sequence49 – 9042Missing in isoform 2.
VSP_047537

Experimental info

Mutagenesis241C → A: Loss of deubiquitination activity, no change in subcellular location. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: A2832B4D9BD675E9

FASTA18820,756
        10         20         30         40         50         60 
MSQAPGAQPS PPTVYHERQR LELCAVHALN NVLQQQLFSQ EAADEICKRL APDSRLNPHR 

        70         80         90        100        110        120 
SLLGTGNYDV NVIMAALQGL GLAAVWWDRR RPLSQLALPQ VLGLILNLPS PVSLGLLSLP 

       130        140        150        160        170        180 
LRRRHWVALR QVDGVYYNLD SKLRAPEALG DEDGVRAFLA AALAQGLCEV LLVVTKEVEE 


KGSWLRTD 

« Hide

Isoform 2 [UniParc].

Checksum: 86386376B0F16FDB
Show »

FASTA14616,100

References

« Hide 'large scale' references
[1]"Hypothetical transmembrane protein SBBI54."
Li N., Wan T., Zhang W., Cao X.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Macrophage.
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Uterus.
[6]"Josephin domain-containing proteins from a variety of species are active de-ubiquitination enzymes."
Tzvetkov N., Breuer P.
Biol. Chem. 388:973-978(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, FUNCTION.
[7]"Crystal structure of a Josephin-ubiquitin complex: evolutionary restraints on ataxin-3 deubiquitinating activity."
Weeks S.D., Grasty K.C., Hernandez-Cuebas L., Loll P.J.
J. Biol. Chem. 286:4555-4565(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, FUNCTION.
[8]"JosD1, a membrane-targeted deubiquitinating enzyme, is activated by ubiquitination and regulates membrane dynamics, cell motility, and endocytosis."
Seki T., Gong L., Williams A.J., Sakai N., Todi S.V., Paulson H.L.
J. Biol. Chem. 288:17145-17155(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-24.
[9]"Structural modeling of ataxin-3 reveals distant homology to adaptins."
Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T.
Proteins 50:355-370(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF247787 mRNA. Translation: AAL95692.1.
AK131052 mRNA. No translation available.
AC008743 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW71877.1.
BC062416 mRNA. Translation: AAH62416.1.
CCDSCCDS12797.1. [Q8TAC2-1]
CCDS59413.1. [Q8TAC2-2]
RefSeqNP_001257568.1. NM_001270639.1. [Q8TAC2-1]
NP_001257569.1. NM_001270640.1. [Q8TAC2-1]
NP_001257570.1. NM_001270641.1. [Q8TAC2-2]
NP_001257615.1. NM_001270686.1. [Q8TAC2-1]
NP_612207.1. NM_138334.3. [Q8TAC2-1]
UniGeneHs.467151.
Hs.737597.

3D structure databases

ProteinModelPortalQ8TAC2.
SMRQ8TAC2. Positions 14-151.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125956. 8 interactions.
STRING9606.ENSP00000293431.

Protein family/group databases

MEROPSC86.005.

PTM databases

PhosphoSiteQ8TAC2.

Polymorphism databases

DMDM29840785.

Proteomic databases

MaxQBQ8TAC2.
PaxDbQ8TAC2.
PRIDEQ8TAC2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000391815; ENSP00000375691; ENSG00000161677. [Q8TAC2-1]
ENST00000595669; ENSP00000468860; ENSG00000161677. [Q8TAC2-2]
ENST00000598418; ENSP00000468956; ENSG00000161677. [Q8TAC2-1]
ENST00000601423; ENSP00000472116; ENSG00000161677. [Q8TAC2-1]
GeneID126119.
KEGGhsa:126119.
UCSCuc002psn.2. human. [Q8TAC2-1]

Organism-specific databases

CTD126119.
GeneCardsGC19M051009.
HGNCHGNC:28853. JOSD2.
MIM615324. gene.
neXtProtNX_Q8TAC2.
PharmGKBPA142671645.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG321724.
HOVERGENHBG039523.
InParanoidQ8TAC2.
KOK15235.
OMAAVWWDRR.
OrthoDBEOG75B871.
PhylomeDBQ8TAC2.
TreeFamTF313660.

Gene expression databases

ArrayExpressQ8TAC2.
BgeeQ8TAC2.
CleanExHS_JOSD2.
GenevestigatorQ8TAC2.

Family and domain databases

InterProIPR006155. Josephin.
[Graphical view]
PfamPF02099. Josephin. 1 hit.
[Graphical view]
PRINTSPR01233. JOSEPHIN.
PROSITEPS50957. JOSEPHIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSJOSD2. human.
GenomeRNAi126119.
NextBio81688.
PROQ8TAC2.
SOURCESearch...

Entry information

Entry nameJOS2_HUMAN
AccessionPrimary (citable) accession number: Q8TAC2
Secondary accession number(s): M0QX25
Entry history
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM