Q8TAC2 (JOS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Josephin-2
EC=3.4.19.12

Alternative name(s):
Josephin domain-containing protein 2

Gene names

Name:	JOSD2
ORF Names:	SBBI54

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme. Ref.3 Ref.4
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.3 Ref.4
Sequence similarities	Contains 1 Josephin domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from direct assay Ref.4. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	omega peptidase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Josephin-2		PRO_0000053843
Regions
	Domain	11 – 188	178	Josephin
Sites
	Active site	24	1	Nucleophile By similarity
	Active site	125	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TAC2 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A2832B4D9BD675E9	FASTA	188	20,756
	10 20 30 40 50 60 MSQAPGAQPS PPTVYHERQR LELCAVHALN NVLQQQLFSQ EAADEICKRL APDSRLNPHR 70 80 90 100 110 120 SLLGTGNYDV NVIMAALQGL GLAAVWWDRR RPLSQLALPQ VLGLILNLPS PVSLGLLSLP 130 140 150 160 170 180 LRRRHWVALR QVDGVYYNLD SKLRAPEALG DEDGVRAFLA AALAQGLCEV LLVVTKEVEE KGSWLRTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Hypothetical transmembrane protein SBBI54." Li N., Wan T., Zhang W., Cao X. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[3]	"Josephin domain-containing proteins from a variety of species are active de-ubiquitination enzymes." Tzvetkov N., Breuer P. Biol. Chem. 388:973-978(2007) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, FUNCTION.
[4]	"Crystal structure of a Josephin-ubiquitin complex: evolutionary restraints on ataxin-3 deubiquitinating activity." Weeks S.D., Grasty K.C., Hernandez-Cuebas L., Loll P.J. J. Biol. Chem. 286:4555-4565(2011) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, FUNCTION.
[5]	"Structural modeling of ataxin-3 reveals distant homology to adaptins." Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T. Proteins 50:355-370(2003) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF247787 mRNA. Translation: AAL95692.1. BC062416 mRNA. Translation: AAH62416.1.
IPI	IPI00152032.
RefSeq	NP_001257568.1. NM_001270639.1. NP_001257569.1. NM_001270640.1. NP_001257615.1. NM_001270686.1. NP_612207.1. NM_138334.3.
UniGene	Hs.467151. Hs.737597.
3D structure databases
ProteinModelPortal	Q8TAC2.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000293431.
Protein family/group databases
MEROPS	C86.005.
PTM databases
PhosphoSite	Q8TAC2.
Polymorphism databases
DMDM	29840785.
Proteomic databases
PaxDb	Q8TAC2.
PRIDE	Q8TAC2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000391815; ENSP00000375691; ENSG00000161677. ENST00000598418; ENSP00000468956; ENSG00000161677. ENST00000601423; ENSP00000472116; ENSG00000161677.
GeneID	126119.
KEGG	hsa:126119.
UCSC	uc002psn.1. human.
Organism-specific databases
CTD	126119.
GeneCards	GC19M051009.
HGNC	HGNC:28853. JOSD2.
neXtProt	NX_Q8TAC2.
PharmGKB	PA142671645.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG321724.
HOVERGEN	HBG039523.
InParanoid	Q8TAC2.
KO	K15235.
OrthoDB	EOG44QT25.
PhylomeDB	Q8TAC2.
Gene expression databases
ArrayExpress	Q8TAC2.
Bgee	Q8TAC2.
CleanEx	HS_JOSD2.
Genevestigator	Q8TAC2.
GermOnline	ENSG00000161677. Homo sapiens.
Family and domain databases
InterPro	IPR006155. Josephin. [Graphical view]
Pfam	PF02099. Josephin. 1 hit. [Graphical view]
PRINTS	PR01233. JOSEPHIN.
PROSITE	PS50957. JOSEPHIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	JOSD2. human.
GenomeRNAi	126119.
NextBio	81688.

Entry information

Entry name

JOS2_HUMAN

Accession

Primary (citable) accession number: Q8TAC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.