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Protein

Proteasome subunit alpha-type 8

Gene

PSMA8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the spermatoproteasome, a form of the proteasome specifically found in testis that promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiT01.974

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha-type 8 (EC:3.4.25.1)
Alternative name(s):
Proteasome subunit alpha-type 7-like
Gene namesi
Name:PSMA8
Synonyms:PSMA7L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000154611.14
HGNCiHGNC:22985 PSMA8
MIMi617841 gene
neXtProtiNX_Q8TAA3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000154611
PharmGKBiPA134969470

Chemistry databases

ChEMBLiCHEMBL3831201

Polymorphism and mutation databases

BioMutaiPSMA8
DMDMi108936006

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241491 – 256Proteasome subunit alpha-type 8Add BLAST256

Proteomic databases

MaxQBiQ8TAA3
PaxDbiQ8TAA3
PeptideAtlasiQ8TAA3
PRIDEiQ8TAA3

PTM databases

iPTMnetiQ8TAA3
PhosphoSitePlusiQ8TAA3

Expressioni

Gene expression databases

BgeeiENSG00000154611
CleanExiHS_PSMA8
ExpressionAtlasiQ8TAA3 baseline and differential
GenevisibleiQ8TAA3 HS

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity). Component of the spermatoproteasome, a form of the proteasome specifically found in testis.By similarity

Protein-protein interaction databases

BioGridi126804, 36 interactors
IntActiQ8TAA3, 1 interactor
STRINGi9606.ENSP00000311121

Chemistry databases

BindingDBiQ8TAA3

Structurei

3D structure databases

ProteinModelPortaliQ8TAA3
SMRiQ8TAA3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0183 Eukaryota
ENOG410XP21 LUCA
GeneTreeiENSGT00550000074753
HOGENOMiHOG000091085
HOVERGENiHBG003005
InParanoidiQ8TAA3
KOiK02731
OMAiEVAIMEN
OrthoDBiEOG091G0GX6
PhylomeDBiQ8TAA3
TreeFamiTF106212

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR037560 Proteasome_alpha_type_7
IPR000426 Proteasome_asu_N
IPR001353 Proteasome_sua/b
PANTHERiPTHR11599:SF47 PTHR11599:SF47, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q8TAA3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASRYDRAIT VFSPDGHLFQ VEYAQEAVKK GSTAVGIRGT NIVVLGVEKK
60 70 80 90 100
SVAKLQDERT VRKICALDDH VCMAFAVLTI FIGLTADARV VINRARVECQ
110 120 130 140 150
SHKLTVEDPV TVEYITRFIA TLKQKYTQSN GRRPFGISAL IVGFDDDGIS
160 170 180 190 200
RLYQTDPSGT YHAWKANAIG RSAKTVREFL EKNYTEDAIA SDSEAIKLAI
210 220 230 240 250
KALLEVVQSG GKNIELAIIR RNQPLKMFSA KEVELYVTEI EKEKEEAEKK

KSKKSV
Note: Predicted but not yet described.
Length:256
Mass (Da):28,530
Last modified:May 30, 2006 - v3
Checksum:i6DD04535A98BE44A
GO
Isoform 2 (identifier: Q8TAA3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     40-83: Missing.

Show »
Length:212
Mass (Da):23,702
Checksum:i6C361094EFCC4BB2
GO
Isoform 3 (identifier: Q8TAA3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-82: Missing.

Note: No experimental confirmation available.
Show »
Length:250
Mass (Da):27,843
Checksum:iC204707CF06D6D17
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti212K → R in AAH28371 (PubMed:15489334).Curated1
Sequence conflicti212K → R in AAH28686 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00528540 – 83Missing in isoform 2. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_01860277 – 82Missing in isoform 3. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF445014 Genomic DNA Translation: ACA06053.1
AC091021 Genomic DNA No translation available.
AC016839 Genomic DNA No translation available.
CH471088 Genomic DNA Translation: EAX01214.1
BC028371 mRNA Translation: AAH28371.1
BC028686 mRNA Translation: AAH28686.1
BC042820 mRNA Translation: AAH42820.1
CCDSiCCDS32808.1 [Q8TAA3-1]
CCDS45842.1 [Q8TAA3-5]
CCDS45843.1 [Q8TAA3-2]
RefSeqiNP_001020267.1, NM_001025096.1 [Q8TAA3-5]
NP_001020268.1, NM_001025097.1 [Q8TAA3-2]
NP_653263.2, NM_144662.2 [Q8TAA3-1]
UniGeneiHs.464813

Genome annotation databases

EnsembliENST00000308268; ENSP00000311121; ENSG00000154611 [Q8TAA3-1]
ENST00000343848; ENSP00000345584; ENSG00000154611 [Q8TAA3-2]
ENST00000415576; ENSP00000409284; ENSG00000154611 [Q8TAA3-5]
GeneIDi143471
KEGGihsa:143471
UCSCiuc002kvo.4 human [Q8TAA3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSMA8_HUMAN
AccessioniPrimary (citable) accession number: Q8TAA3
Secondary accession number(s): B0YJ75
, Q8IVP4, Q8TA98, Q8TAA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: May 30, 2006
Last modified: March 28, 2018
This is version 141 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health