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Protein

Proteasome subunit alpha type-7-like

Gene

PSMA8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the spermatoproteasome, a form of the proteasome specifically found in testis that promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_11063. Degradation of beta-catenin by the destruction complex.
REACT_111056. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_111178. ER-Phagosome pathway.
REACT_1156. Orc1 removal from chromatin.
REACT_118656. Activation of NF-kappaB in B cells.
REACT_1221. CDK-mediated phosphorylation and removal of Cdc6.
REACT_13464. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_13565. Regulation of ornithine decarboxylase (ODC).
REACT_150471. Separation of Sister Chromatids.
REACT_1614. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_188323. CLEC7A (Dectin-1) signaling.
REACT_188330. Dectin-1 mediated noncanonical NF-kB signaling.
REACT_1949. CDT1 association with the CDC6:ORC:origin complex.
REACT_20549. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_25325. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_263873. degradation of AXIN.
REACT_263883. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
REACT_264438. degradation of DVL.
REACT_264478. Asymmetric localization of PCP proteins.
REACT_264605. Hedgehog ligand biogenesis.
REACT_267700. Degradation of GLI2 by the proteasome.
REACT_268156. Degradation of GLI1 by the proteasome.
REACT_268366. GLI3 is processed to GLI3R by the proteasome.
REACT_268718. Hedgehog 'on' state.
REACT_4. Ubiquitin-dependent degradation of Cyclin D1.
REACT_6761. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_6821. SCF-beta-TrCP mediated degradation of Emi1.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_6871. APC/C:Cdc20 mediated degradation of Securin.
REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_9031. Vpu mediated degradation of CD4.
REACT_9453. Vif-mediated degradation of APOBEC3G.

Protein family/group databases

MEROPSiT01.974.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-7-like (EC:3.4.25.1)
Gene namesi
Name:PSMA8
Synonyms:PSMA7L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:22985. PSMA8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • nucleus Source: UniProtKB
  • proteasome core complex Source: GO_Central
  • proteasome core complex, alpha-subunit complex Source: InterPro
  • spermatoproteasome complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134969470.

Polymorphism and mutation databases

BioMutaiPSMA8.
DMDMi108936006.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Proteasome subunit alpha type-7-likePRO_0000124149Add
BLAST

Proteomic databases

MaxQBiQ8TAA3.
PaxDbiQ8TAA3.
PRIDEiQ8TAA3.

PTM databases

PhosphoSiteiQ8TAA3.

Expressioni

Gene expression databases

BgeeiQ8TAA3.
CleanExiHS_PSMA8.
ExpressionAtlasiQ8TAA3. baseline and differential.
GenevisibleiQ8TAA3. HS.

Organism-specific databases

HPAiCAB013455.
HPA049377.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity). Component of the spermatoproteasome, a form of the proteasome specifically found in testis.By similarity

Protein-protein interaction databases

BioGridi126804. 33 interactions.
STRINGi9606.ENSP00000311121.

Structurei

3D structure databases

ProteinModelPortaliQ8TAA3.
SMRiQ8TAA3. Positions 5-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00550000074753.
HOGENOMiHOG000091085.
HOVERGENiHBG003005.
InParanoidiQ8TAA3.
KOiK02731.
OMAiIQEYTQT.
OrthoDBiEOG71VSTG.
PhylomeDBiQ8TAA3.
TreeFamiTF106212.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q8TAA3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASRYDRAIT VFSPDGHLFQ VEYAQEAVKK GSTAVGIRGT NIVVLGVEKK
60 70 80 90 100
SVAKLQDERT VRKICALDDH VCMAFAVLTI FIGLTADARV VINRARVECQ
110 120 130 140 150
SHKLTVEDPV TVEYITRFIA TLKQKYTQSN GRRPFGISAL IVGFDDDGIS
160 170 180 190 200
RLYQTDPSGT YHAWKANAIG RSAKTVREFL EKNYTEDAIA SDSEAIKLAI
210 220 230 240 250
KALLEVVQSG GKNIELAIIR RNQPLKMFSA KEVELYVTEI EKEKEEAEKK

KSKKSV
Note: Predicted but not yet described.
Length:256
Mass (Da):28,530
Last modified:May 30, 2006 - v3
Checksum:i6DD04535A98BE44A
GO
Isoform 2 (identifier: Q8TAA3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     40-83: Missing.

Show »
Length:212
Mass (Da):23,702
Checksum:i6C361094EFCC4BB2
GO
Isoform 3 (identifier: Q8TAA3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-82: Missing.

Note: No experimental confirmation available.
Show »
Length:250
Mass (Da):27,843
Checksum:iC204707CF06D6D17
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti212 – 2121K → R in AAH28371 (PubMed:15489334).Curated
Sequence conflicti212 – 2121K → R in AAH28686 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei40 – 8344Missing in isoform 2. 1 PublicationVSP_005285Add
BLAST
Alternative sequencei77 – 826Missing in isoform 3. 1 PublicationVSP_018602

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF445014 Genomic DNA. Translation: ACA06053.1.
AC091021 Genomic DNA. No translation available.
AC016839 Genomic DNA. No translation available.
CH471088 Genomic DNA. Translation: EAX01214.1.
BC028371 mRNA. Translation: AAH28371.1.
BC028686 mRNA. Translation: AAH28686.1.
BC042820 mRNA. Translation: AAH42820.1.
CCDSiCCDS32808.1. [Q8TAA3-1]
CCDS45842.1. [Q8TAA3-5]
CCDS45843.1. [Q8TAA3-2]
RefSeqiNP_001020267.1. NM_001025096.1. [Q8TAA3-5]
NP_001020268.1. NM_001025097.1. [Q8TAA3-2]
NP_653263.2. NM_144662.2. [Q8TAA3-1]
UniGeneiHs.464813.

Genome annotation databases

EnsembliENST00000308268; ENSP00000311121; ENSG00000154611.
ENST00000343848; ENSP00000345584; ENSG00000154611. [Q8TAA3-2]
ENST00000415576; ENSP00000409284; ENSG00000154611. [Q8TAA3-5]
GeneIDi143471.
KEGGihsa:143471.
UCSCiuc002kvo.3. human. [Q8TAA3-2]
uc002kvp.3. human. [Q8TAA3-5]
uc002kvq.3. human. [Q8TAA3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF445014 Genomic DNA. Translation: ACA06053.1.
AC091021 Genomic DNA. No translation available.
AC016839 Genomic DNA. No translation available.
CH471088 Genomic DNA. Translation: EAX01214.1.
BC028371 mRNA. Translation: AAH28371.1.
BC028686 mRNA. Translation: AAH28686.1.
BC042820 mRNA. Translation: AAH42820.1.
CCDSiCCDS32808.1. [Q8TAA3-1]
CCDS45842.1. [Q8TAA3-5]
CCDS45843.1. [Q8TAA3-2]
RefSeqiNP_001020267.1. NM_001025096.1. [Q8TAA3-5]
NP_001020268.1. NM_001025097.1. [Q8TAA3-2]
NP_653263.2. NM_144662.2. [Q8TAA3-1]
UniGeneiHs.464813.

3D structure databases

ProteinModelPortaliQ8TAA3.
SMRiQ8TAA3. Positions 5-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126804. 33 interactions.
STRINGi9606.ENSP00000311121.

Chemistry

ChEMBLiCHEMBL2364701.

Protein family/group databases

MEROPSiT01.974.

PTM databases

PhosphoSiteiQ8TAA3.

Polymorphism and mutation databases

BioMutaiPSMA8.
DMDMi108936006.

Proteomic databases

MaxQBiQ8TAA3.
PaxDbiQ8TAA3.
PRIDEiQ8TAA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000308268; ENSP00000311121; ENSG00000154611.
ENST00000343848; ENSP00000345584; ENSG00000154611. [Q8TAA3-2]
ENST00000415576; ENSP00000409284; ENSG00000154611. [Q8TAA3-5]
GeneIDi143471.
KEGGihsa:143471.
UCSCiuc002kvo.3. human. [Q8TAA3-2]
uc002kvp.3. human. [Q8TAA3-5]
uc002kvq.3. human. [Q8TAA3-1]

Organism-specific databases

CTDi143471.
GeneCardsiGC18P023713.
HGNCiHGNC:22985. PSMA8.
HPAiCAB013455.
HPA049377.
neXtProtiNX_Q8TAA3.
PharmGKBiPA134969470.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00550000074753.
HOGENOMiHOG000091085.
HOVERGENiHBG003005.
InParanoidiQ8TAA3.
KOiK02731.
OMAiIQEYTQT.
OrthoDBiEOG71VSTG.
PhylomeDBiQ8TAA3.
TreeFamiTF106212.

Enzyme and pathway databases

ReactomeiREACT_11063. Degradation of beta-catenin by the destruction complex.
REACT_111056. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_111178. ER-Phagosome pathway.
REACT_1156. Orc1 removal from chromatin.
REACT_118656. Activation of NF-kappaB in B cells.
REACT_1221. CDK-mediated phosphorylation and removal of Cdc6.
REACT_13464. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_13565. Regulation of ornithine decarboxylase (ODC).
REACT_150471. Separation of Sister Chromatids.
REACT_1614. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_188323. CLEC7A (Dectin-1) signaling.
REACT_188330. Dectin-1 mediated noncanonical NF-kB signaling.
REACT_1949. CDT1 association with the CDC6:ORC:origin complex.
REACT_20549. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_25325. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_263873. degradation of AXIN.
REACT_263883. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
REACT_264438. degradation of DVL.
REACT_264478. Asymmetric localization of PCP proteins.
REACT_264605. Hedgehog ligand biogenesis.
REACT_267700. Degradation of GLI2 by the proteasome.
REACT_268156. Degradation of GLI1 by the proteasome.
REACT_268366. GLI3 is processed to GLI3R by the proteasome.
REACT_268718. Hedgehog 'on' state.
REACT_4. Ubiquitin-dependent degradation of Cyclin D1.
REACT_6761. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_6821. SCF-beta-TrCP mediated degradation of Emi1.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_6871. APC/C:Cdc20 mediated degradation of Securin.
REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_9031. Vpu mediated degradation of CD4.
REACT_9453. Vif-mediated degradation of APOBEC3G.

Miscellaneous databases

GenomeRNAii143471.
NextBioi84715.
PROiQ8TAA3.

Gene expression databases

BgeeiQ8TAA3.
CleanExiHS_PSMA8.
ExpressionAtlasiQ8TAA3. baseline and differential.
GenevisibleiQ8TAA3. HS.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NHLBI resequencing and genotyping service (RS&G)
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Testis.

Entry informationi

Entry nameiPSA7L_HUMAN
AccessioniPrimary (citable) accession number: Q8TAA3
Secondary accession number(s): B0YJ75
, Q8IVP4, Q8TA98, Q8TAA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: May 30, 2006
Last modified: July 22, 2015
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.