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Protein

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

Gene

pteN

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate (By similarity). Negative regulator of PI3K chemotaxis pathways. Overexpression leads to a suppression of a PI3K-dependent activation of pkbA, and these cells exhibit chemotaxis defects consistent with a reduction in PI3K activity.By similarity7 Publications

Catalytic activityi

Phosphatidylinositol 3,4,5-trisphosphate + H2O = phosphatidylinositol 4,5-bisphosphate + phosphate.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.
Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei124Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • actin filament polymerization Source: dictyBase
  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: dictyBase
  • aggregation involved in sorocarp development Source: dictyBase
  • cell motility Source: dictyBase
  • chemotaxis Source: dictyBase
  • chemotaxis to cAMP Source: dictyBase
  • establishment of cell polarity Source: dictyBase
  • mitotic cytokinesis Source: dictyBase
  • negative chemotaxis Source: dictyBase
  • negative regulation of protein serine/threonine kinase activity Source: dictyBase
  • phosphatidylinositol 3-kinase signaling Source: dictyBase
  • phosphatidylinositol dephosphorylation Source: dictyBase
  • phosphatidylinositol-mediated signaling Source: dictyBase
  • positive regulation of cellular component movement Source: dictyBase
  • protein localization to trailing edge Source: dictyBase
  • protein localization to uropod Source: dictyBase
  • pseudopodium assembly Source: dictyBase
  • regulation of adenylate cyclase activity Source: dictyBase
  • regulation of cell morphogenesis Source: dictyBase
  • regulation of pseudopodium assembly Source: dictyBase
  • uropod retraction Source: dictyBase

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle, Chemotaxis, Lipid metabolism
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-DDI-1660499 Synthesis of PIPs at the plasma membrane
R-DDI-1855204 Synthesis of IP3 and IP4 in the cytosol
R-DDI-199418 Negative regulation of the PI3K/AKT network
R-DDI-202424 Downstream TCR signaling
R-DDI-5689880 Ub-specific processing proteases
R-DDI-5689896 Ovarian tumor domain proteases
R-DDI-8948747 Regulation of PTEN localization
R-DDI-8948751 Regulation of PTEN stability and activity

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (EC:3.1.3.16, EC:3.1.3.48, EC:3.1.3.67)
Alternative name(s):
Pten 3-phosphoinositide phosphatase alpha
Gene namesi
Name:pteN
Synonyms:ptenA
ORF Names:DDB_G0286557
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0286557 pten

Subcellular locationi

GO - Cellular componenti

  • cell cortex of cell tip Source: dictyBase
  • cell trailing edge Source: dictyBase
  • cell trailing edge membrane Source: dictyBase
  • cytosol Source: dictyBase
  • equatorial cell cortex Source: dictyBase
  • plasma membrane Source: dictyBase
  • uropod Source: dictyBase

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Growth defect. Failure to aggregate. Slower migration. Leads to increased F-actin polymerization. Unable to suppress lateral pseudopod formation and turning. Having a direct on PI(3,4,5)P3/PI(3,4)P2 levels. Significant increase in chemoattractant-mediated activation of pkbA and a decrease in chemotaxis speed.5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003768251 – 533Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTENAdd BLAST533

Proteomic databases

PaxDbiQ8T9S7
PRIDEiQ8T9S7

Expressioni

Developmental stagei

In chemotaxing cells, is on the plasma membrane along the lateral sides and posterior of the cell but is absent or the level is significantly reduced at the leading edge.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi1250719, 3 interactors
STRINGi44689.DDB0191093

Structurei

3D structure databases

ProteinModelPortaliQ8T9S7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 185Phosphatase tensin-typePROSITE-ProRule annotationAdd BLAST172
Domaini271 – 406C2 tensin-typePROSITE-ProRule annotationAdd BLAST136

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi237 – 240Poly-Lys4
Compositional biasi266 – 272Poly-Gln7
Compositional biasi455 – 463Poly-Asn9
Compositional biasi471 – 475Poly-Thr5

Phylogenomic databases

eggNOGiKOG2283 Eukaryota
COG2453 LUCA
InParanoidiQ8T9S7
KOiK01110
OMAiGLMICCW
PhylomeDBiQ8T9S7

Family and domain databases

Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR029021 Prot-tyrosine_phosphatase-like
IPR014020 Tensin_C2-dom
IPR029023 Tensin_phosphatase
IPR016130 Tyr_Pase_AS
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF10409 PTEN_C2, 1 hit
SMARTiView protein in SMART
SM01326 PTEN_C2, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS51182 C2_TENSIN, 1 hit
PS51181 PPASE_TENSIN, 1 hit
PS00383 TYR_PHOSPHATASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q8T9S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLLRVAVS KQKRRYQKNG YDLDLAYITD NIVAMGFPSE KVEGVFRNPM
60 70 80 90 100
KDVQRFLDQY HKDHFKVYNL CSERVYDHSK FYGRVGYYPF DDHNAPQFEM
110 120 130 140 150
IDAFCRDVDA WMKEDSKNIA VIHCKAGKGR TGLMICCWLM YCGMWKNTED
160 170 180 190 200
SLRFYAALRT YNQKGVTIPS QIRYVGYFGR SIRESIKYVP RNVTLKKIVL
210 220 230 240 250
RPLPKEINLS EVQFNISVGK NCVFNSKEHN MNVVISKKKK TVVDKNKKDP
260 270 280 290 300
KKKLTKENSE KNIDSQQQQQ SQSSLSQSQQ GQSSPNMQSL SASGTISSGS
310 320 330 340 350
NVGTVNGNTL HQLGGSQFSL SDLADGNTIG NDEYISFEIG ALSLAGDIRI
360 370 380 390 400
EFTNKQDDRM FMFWVNTSFV QQLEIIPKSG LDKAHKDKNH KAFPEDFHVE
410 420 430 440 450
LTFDQLDQQQ SHTTVVASAE EQTNNQHYPQ SSNNVATSSS HHDNITVVAS
460 470 480 490 500
DAPQNNNNNN NLNSSNSNNA TTTTTKNNIS LASSQSNPVQ QESNPSTTTQ
510 520 530
VSEENSAPKV EAEKIENSNA SANDSETSSN SSS
Length:533
Mass (Da):59,831
Last modified:June 1, 2002 - v1
Checksum:i9922F7887D6F9F6A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti515I → N in AAL99958 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF467431 mRNA Translation: AAL75566.1
AF483827 mRNA Translation: AAL99958.1
AAFI02000089 Genomic DNA Translation: EAL64034.1
RefSeqiXP_637576.1, XM_632484.1

Genome annotation databases

EnsemblProtistsiEAL64034; EAL64034; DDB_G0286557
GeneIDi8625716
KEGGiddi:DDB_G0286557

Similar proteinsi

Entry informationi

Entry nameiPTEN_DICDI
AccessioniPrimary (citable) accession number: Q8T9S7
Secondary accession number(s): Q54LI5, Q8T658
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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