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Protein

LDLR chaperone boca

Gene

boca

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wg pathway, since some LDLRs are coreceptors for the canonical Wnt pathway.1 Publication

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: FlyBase
  2. low-density lipoprotein receptor particle metabolic process Source: FlyBase
  3. oogenesis Source: FlyBase
  4. proboscis development Source: FlyBase
  5. protein targeting to membrane Source: FlyBase
  6. Wnt signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
LDLR chaperone boca
Gene namesi
Name:boca
ORF Names:CG30498
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0004132. boca.

Subcellular locationi

Endoplasmic reticulum 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. apical part of cell Source: FlyBase
  2. cytoplasm Source: FlyBase
  3. endomembrane system Source: FlyBase
  4. endoplasmic reticulum Source: FlyBase
  5. endoplasmic reticulum lumen Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi49 – 491W → R in boca1; induces lethality. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 180162LDLR chaperone bocaPRO_0000385021Add
BLAST

Proteomic databases

PaxDbiQ8T9B6.
PRIDEiQ8T9B6.

Expressioni

Gene expression databases

BgeeiQ8T9B6.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with Arrow and Yolkless.By similarity1 Publication

Protein-protein interaction databases

BioGridi71878. 74 interactions.
DIPiDIP-59112N.
STRINGi7227.FBpp0088027.

Structurei

Secondary structure

1
180
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi94 – 10310Combined sources
Helixi106 – 12217Combined sources
Beta strandi127 – 1337Combined sources
Beta strandi136 – 1438Combined sources
Helixi144 – 1463Combined sources
Helixi147 – 1548Combined sources
Beta strandi160 – 1656Combined sources
Beta strandi168 – 1714Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OFEX-ray2.29A/B88-172[»]
3OFFX-ray2.00A89-172[»]
ProteinModelPortaliQ8T9B6.
SMRiQ8T9B6. Positions 31-172.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8T9B6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 16674Structured coreBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi177 – 1804Prevents secretion from ER

Domaini

The LDLR maturation activity resides in the N- and C-terminal unstructured regions.By similarity

Sequence similaritiesi

Belongs to the MESD family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG286690.
GeneTreeiENSGT00390000000993.
InParanoidiQ8T9B6.
OMAiSLWNNHI.
OrthoDBiEOG7XPZ76.
PhylomeDBiQ8T9B6.

Family and domain databases

InterProiIPR019330. Mesoderm_development_cand-2.
[Graphical view]
PfamiPF10185. Mesd. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8T9B6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTRLVLLLL ALTPLVLAKK FKEEEKPAWA KKDIRDYSEA DLERLLDQWE
60 70 80 90 100
EDEEPLEDDE LPEHLRPQPK LDLSNLDSKS PEDLLKVSKK GRTLMTFVSV
110 120 130 140 150
TGNPTREESD TITKLWQTSL WNNHIQAERY MVDDNRAIFL FKDGTQAWDA
160 170 180
KDFLIEQERC KGVTIENKEY PGVNAKKDEL
Length:180
Mass (Da):21,002
Last modified:September 22, 2009 - v2
Checksum:iB51E057C901A6546
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151L → V in AAL39985 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF59229.2.
AY069840 mRNA. Translation: AAL39985.1.
BT044294 mRNA. Translation: ACH92359.1.
RefSeqiNP_724578.1. NM_165541.2.
UniGeneiDm.14878.

Genome annotation databases

EnsemblMetazoaiFBtr0088953; FBpp0088027; FBgn0004132.
GeneIDi48986.
KEGGidme:Dmel_CG30498.
UCSCiCG30498-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF59229.2.
AY069840 mRNA. Translation: AAL39985.1.
BT044294 mRNA. Translation: ACH92359.1.
RefSeqiNP_724578.1. NM_165541.2.
UniGeneiDm.14878.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OFEX-ray2.29A/B88-172[»]
3OFFX-ray2.00A89-172[»]
ProteinModelPortaliQ8T9B6.
SMRiQ8T9B6. Positions 31-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi71878. 74 interactions.
DIPiDIP-59112N.
STRINGi7227.FBpp0088027.

Proteomic databases

PaxDbiQ8T9B6.
PRIDEiQ8T9B6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088953; FBpp0088027; FBgn0004132.
GeneIDi48986.
KEGGidme:Dmel_CG30498.
UCSCiCG30498-RA. d. melanogaster.

Organism-specific databases

CTDi48986.
FlyBaseiFBgn0004132. boca.

Phylogenomic databases

eggNOGiNOG286690.
GeneTreeiENSGT00390000000993.
InParanoidiQ8T9B6.
OMAiSLWNNHI.
OrthoDBiEOG7XPZ76.
PhylomeDBiQ8T9B6.

Miscellaneous databases

EvolutionaryTraceiQ8T9B6.
GenomeRNAii48986.
NextBioi839609.
PROiQ8T9B6.

Gene expression databases

BgeeiQ8T9B6.

Family and domain databases

InterProiIPR019330. Mesoderm_development_cand-2.
[Graphical view]
PfamiPF10185. Mesd. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  4. "Boca, an endoplasmic reticulum protein required for wingless signaling and trafficking of LDL receptor family members in Drosophila."
    Culi J., Mann R.S.
    Cell 112:343-354(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ARROW AND YOLKLESS, SUBCELLULAR LOCATION, MUTAGENESIS OF TRP-49.

Entry informationi

Entry nameiMESD_DROME
AccessioniPrimary (citable) accession number: Q8T9B6
Secondary accession number(s): A1Z712
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: January 7, 2015
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.