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Q8T8C0

- NOS_BOMMO

UniProt

Q8T8C0 - NOS_BOMMO

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Protein

Nitric oxide synthase

Gene

NOS

Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Involved in the induction of immune gene expression.1 PublicationCurated

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity

Enzyme regulationi

Expression is dependent on and stimulated by NADPH, calcium, BH4 and calmodulin. The activity is not dependent on FAD and is not stimulated by its presence.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi181 – 1811Iron (heme axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi527 – 5315FMNPROSITE-ProRule annotation
Nucleotide bindingi669 – 70032FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi811 – 82212FADBy similarityAdd
BLAST
Nucleotide bindingi954 – 96411FADBy similarityAdd
BLAST
Nucleotide bindingi1028 – 1147120NADPBy similarityAdd
BLAST
Nucleotide bindingi1128 – 114316NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADP binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthaseImported (EC:1.14.13.39)
Alternative name(s):
Inducible nitric oxide synthase-like protein
Gene namesi
Name:NOSImported
Synonyms:iNOS-LPImported
OrganismiBombyx mori (Silk moth)
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
ProteomesiUP000005204: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12091209Nitric oxide synthasePRO_0000403313Add
BLAST

Proteomic databases

PRIDEiQ8T8C0.

Expressioni

Tissue specificityi

Constitutively expressed at a low level in the larval fat body, hemocyte, Malpighian tubule, midgut, silk gland and adult antenna.1 Publication

Inductioni

Induced strongly in the fat body by lipopolysaccharide (LPS).1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8T8C0.
SMRiQ8T8C0. Positions 63-482, 518-594.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini521 – 723203Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini776 – 1021246FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni491 – 51121Calmodulin-bindingBy similaritySequence AnalysisAdd
BLAST
Regioni516 – 53520Tetrahydrobiopterin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.Sequence Analysis
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiQ8T8C0.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8T8C0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNFFKICCV KDPGGSAKEN LRKMDQVNGH FVSATCPFSG ESVELKVDSN
60 70 80 90 100
QKQVKPNLRI KVPQPIRLKN HVAHDENFDT LHSRINEVTH FNTKCSEKVC
110 120 130 140 150
QTSIMDIPGR GDTPRTAEEV FKDAQAFLTQ YYASIKRENS EAHKARLDEV
160 170 180 190 200
KRELKERGTY QLKTSELVFG AKLAWRNATR CIGRIQWKKL QIFDCREVTT
210 220 230 240 250
ASGMFEALCN HIKYATNKGN IRSAITIFPQ RTDGKHDYRI WNPQLINYAG
260 270 280 290 300
YQEPDGSITG DPARVEFTEI CMKLGWKAPR TPWDILPLVL SADGKDPEFF
310 320 330 340 350
ELPKEIVMEV QFEHPEYDWF KDMGWKWYAL PAVSNMRLAC GGLEFTANSF
360 370 380 390 400
NGWYMGTEIG CRNLCDESRL NIVEAVAKKM GLDTNSFVSL WKDKALVEVN
410 420 430 440 450
IAVLHSFHRD NVSIVDHHSA SEQFQKHLEN ENKSRGGCPA DWIWIVPPMS
460 470 480 490 500
SSLTTVFHQE MALYYIRPSY DYQEPPWKTH QWTKSDGTKT VHRKFHFKQI
510 520 530 540 550
ARAVKFTSKL FGRALSKRIK ATILYATETG KSEHYAKELG TIFGHAFNAQ
560 570 580 590 600
VHCMSEYDMF SIEHETLVLI VASTFGNGEP PANGVDFAEH LFQMLYNETN
610 620 630 640 650
NNRGDGTSDL GSGTFKTPTP KSLMRSNSMM TPSFEYKRQL TRLESNKSSV
660 670 680 690 700
AGASSIEQIG PLSNVCFAVF GLGSSAYPKF CHFGKTVDKI LGDLGGERIL
710 720 730 740 750
ELACGDELYG QEQQFRAWSS KIFQVACETF CLDENGMVKD AKKALGDVPL
760 770 780 790 800
TEETVRFGKY QGDTKLKAAL EASFRKQLIT CKVKENKNLG DFSADRATVF
810 820 830 840 850
VDMQPETEFK YDPGDHVGVL ACNRKEIVDA VLERMKDVDD YDKTVQLQVM
860 870 880 890 900
KETLTPTGAI KTWEQHERLP AVTIRQIFTR FLDITTPPST TVLKYLSKAC
910 920 930 940 950
TDQNDAAQLK ELAIDSNKYD DWRHLHYPHL AEVLAQFPSC RPQASLLAAL
960 970 980 990 1000
LPSLQPRFYS ISSSPLAHPH RIHITCAVVV YRTQNGQGPM HYGVCSTYLQ
1010 1020 1030 1040 1050
NLKPEDEALV FIRRAPSFHM PKDLSAPLIL VGPGSGIAPF RGFWHHRKHQ
1060 1070 1080 1090 1100
IKNLIPNKQK PGPVWLFFGC RNRGMDLYKE EKEKALADGV LSKVFLALSR
1110 1120 1130 1140 1150
EDSVEKKHIQ TLLEDEGAEI SRMLLDENGH FYVCGDCKMA EEVQQKLKEI
1160 1170 1180 1190 1200
MKKHAKMTEQ EYEEFILNLM DENRYHEDIF GITLRTAEVQ SASREFAKRT

RQESQKSQT
Length:1,209
Mass (Da):137,507
Last modified:June 1, 2002 - v1
Checksum:i92A73F04B12C2E2C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti157 – 1571R → K in BAB33296. 1 PublicationCurated
Sequence conflicti205 – 2051F → L in BAB33296. 1 PublicationCurated
Sequence conflicti259 – 2591T → I in BAB33296. 1 PublicationCurated
Sequence conflicti332 – 3321A → P in BAB33296. 1 PublicationCurated
Sequence conflicti340 – 3412CG → KK in BAB33296. 1 PublicationCurated
Sequence conflicti351 – 3522NG → KN in BAB33296. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071182 mRNA. Translation: BAB85836.1.
AB485775 mRNA. Translation: BAH23563.1.
AB017521 mRNA. Translation: BAB33296.1.
RefSeqiNP_001036963.1. NM_001043498.1.
UniGeneiBmo.133.

Genome annotation databases

GeneIDi692510.
KEGGibmor:692510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071182 mRNA. Translation: BAB85836.1 .
AB485775 mRNA. Translation: BAH23563.1 .
AB017521 mRNA. Translation: BAB33296.1 .
RefSeqi NP_001036963.1. NM_001043498.1.
UniGenei Bmo.133.

3D structure databases

ProteinModelPortali Q8T8C0.
SMRi Q8T8C0. Positions 63-482, 518-594.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q8T8C0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 692510.
KEGGi bmor:692510.

Organism-specific databases

CTDi 4842.

Phylogenomic databases

InParanoidi Q8T8C0.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning, characterization and gene expression of nitric oxide synthase from the silkworm, Bombyx mori."
    Imamura M., Yang J., Yamakawa M.
    Insect Mol. Biol. 11:257-265(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION.
    Strain: Tokai X Asahi1 Publication.
    Tissue: Larva1 Publication.
  2. "Identification of nitric-oxide synthase from Bombyx mori."
    Nagaoka S., Takata Y.
    Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Reproductive systemImported.
  3. "Inducible nitric oxide synthase-like protein(iNOS-LP) cDNA."
    Yang J., Taniai K., Yamakawa M.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-354.

Entry informationi

Entry nameiNOS_BOMMO
AccessioniPrimary (citable) accession number: Q8T8C0
Secondary accession number(s): Q9BLL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3