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Q8T8C0 (NOS_BOMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase

EC=1.14.13.39
Alternative name(s):
Inducible nitric oxide synthase-like protein
Gene names
Name:NOS
Synonyms:iNOS-LP
OrganismBombyx mori (Silk moth) [Reference proteome]
Taxonomic identifier7091 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx

Protein attributes

Sequence length1209 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Involved in the induction of immune gene expression. Ref.1

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity. UniProtKB P29475

Binds 1 FAD By similarity. UniProtKB P29475

Binds 1 FMN By similarity. UniProtKB P29475

Enzyme regulation

Expression is dependent on and stimulated by NADPH, calcium, BH4 and calmodulin. The activity is not dependent on FAD and is not stimulated by its presence. Ref.1

Tissue specificity

Constitutively expressed at a low level in the larval fat body, hemocyte, Malpighian tubule, midgut, silk gland and adult antenna. Ref.1

Induction

Induced strongly in the fat body by lipopolysaccharide (LPS). Ref.1

Sequence similarities

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12091209Nitric oxide synthase
PRO_0000403313

Regions

Domain521 – 723203Flavodoxin-like
Domain776 – 1021246FAD-binding FR-type
Nucleotide binding527 – 5315FMN By similarity
Nucleotide binding669 – 70032FMN By similarity
Nucleotide binding811 – 82212FAD By similarity UniProtKB P29475
Nucleotide binding954 – 96411FAD By similarity UniProtKB P29475
Nucleotide binding1028 – 1147120NADP By similarity UniProtKB P29475
Nucleotide binding1128 – 114316NADP By similarity UniProtKB P29475
Region491 – 51121Calmodulin-binding Potential UniProtKB P29475
Region516 – 53520Tetrahydrobiopterin-binding By similarity UniProtKB P29475

Sites

Metal binding1811Iron (heme axial ligand) By similarity UniProtKB P29475

Experimental info

Sequence conflict1571R → K in BAB33296. Ref.3
Sequence conflict2051F → L in BAB33296. Ref.3
Sequence conflict2591T → I in BAB33296. Ref.3
Sequence conflict3321A → P in BAB33296. Ref.3
Sequence conflict340 – 3412CG → KK in BAB33296. Ref.3
Sequence conflict351 – 3522NG → KN in BAB33296. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8T8C0 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 92A73F04B12C2E2C

FASTA1,209137,507
        10         20         30         40         50         60 
MSNFFKICCV KDPGGSAKEN LRKMDQVNGH FVSATCPFSG ESVELKVDSN QKQVKPNLRI 

        70         80         90        100        110        120 
KVPQPIRLKN HVAHDENFDT LHSRINEVTH FNTKCSEKVC QTSIMDIPGR GDTPRTAEEV 

       130        140        150        160        170        180 
FKDAQAFLTQ YYASIKRENS EAHKARLDEV KRELKERGTY QLKTSELVFG AKLAWRNATR 

       190        200        210        220        230        240 
CIGRIQWKKL QIFDCREVTT ASGMFEALCN HIKYATNKGN IRSAITIFPQ RTDGKHDYRI 

       250        260        270        280        290        300 
WNPQLINYAG YQEPDGSITG DPARVEFTEI CMKLGWKAPR TPWDILPLVL SADGKDPEFF 

       310        320        330        340        350        360 
ELPKEIVMEV QFEHPEYDWF KDMGWKWYAL PAVSNMRLAC GGLEFTANSF NGWYMGTEIG 

       370        380        390        400        410        420 
CRNLCDESRL NIVEAVAKKM GLDTNSFVSL WKDKALVEVN IAVLHSFHRD NVSIVDHHSA 

       430        440        450        460        470        480 
SEQFQKHLEN ENKSRGGCPA DWIWIVPPMS SSLTTVFHQE MALYYIRPSY DYQEPPWKTH 

       490        500        510        520        530        540 
QWTKSDGTKT VHRKFHFKQI ARAVKFTSKL FGRALSKRIK ATILYATETG KSEHYAKELG 

       550        560        570        580        590        600 
TIFGHAFNAQ VHCMSEYDMF SIEHETLVLI VASTFGNGEP PANGVDFAEH LFQMLYNETN 

       610        620        630        640        650        660 
NNRGDGTSDL GSGTFKTPTP KSLMRSNSMM TPSFEYKRQL TRLESNKSSV AGASSIEQIG 

       670        680        690        700        710        720 
PLSNVCFAVF GLGSSAYPKF CHFGKTVDKI LGDLGGERIL ELACGDELYG QEQQFRAWSS 

       730        740        750        760        770        780 
KIFQVACETF CLDENGMVKD AKKALGDVPL TEETVRFGKY QGDTKLKAAL EASFRKQLIT 

       790        800        810        820        830        840 
CKVKENKNLG DFSADRATVF VDMQPETEFK YDPGDHVGVL ACNRKEIVDA VLERMKDVDD 

       850        860        870        880        890        900 
YDKTVQLQVM KETLTPTGAI KTWEQHERLP AVTIRQIFTR FLDITTPPST TVLKYLSKAC 

       910        920        930        940        950        960 
TDQNDAAQLK ELAIDSNKYD DWRHLHYPHL AEVLAQFPSC RPQASLLAAL LPSLQPRFYS 

       970        980        990       1000       1010       1020 
ISSSPLAHPH RIHITCAVVV YRTQNGQGPM HYGVCSTYLQ NLKPEDEALV FIRRAPSFHM 

      1030       1040       1050       1060       1070       1080 
PKDLSAPLIL VGPGSGIAPF RGFWHHRKHQ IKNLIPNKQK PGPVWLFFGC RNRGMDLYKE 

      1090       1100       1110       1120       1130       1140 
EKEKALADGV LSKVFLALSR EDSVEKKHIQ TLLEDEGAEI SRMLLDENGH FYVCGDCKMA 

      1150       1160       1170       1180       1190       1200 
EEVQQKLKEI MKKHAKMTEQ EYEEFILNLM DENRYHEDIF GITLRTAEVQ SASREFAKRT 


RQESQKSQT 

« Hide

References

[1]"cDNA cloning, characterization and gene expression of nitric oxide synthase from the silkworm, Bombyx mori."
Imamura M., Yang J., Yamakawa M.
Insect Mol. Biol. 11:257-265(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION.
Strain: Tokai X Asahi.
Tissue: Larva.
[2]"Identification of nitric-oxide synthase from Bombyx mori."
Nagaoka S., Takata Y.
Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Reproductive system.
[3]"Inducible nitric oxide synthase-like protein(iNOS-LP) cDNA."
Yang J., Taniai K., Yamakawa M.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-354.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB071182 mRNA. Translation: BAB85836.1.
AB485775 mRNA. Translation: BAH23563.1.
AB017521 mRNA. Translation: BAB33296.1.
RefSeqNP_001036963.1. NM_001043498.1.
UniGeneBmo.133.

3D structure databases

ProteinModelPortalQ8T8C0.
SMRQ8T8C0. Positions 63-482, 518-594.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ8T8C0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID692510.
KEGGbmor:692510.

Organism-specific databases

CTD4842.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF000333. NOS. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS_BOMMO
AccessionPrimary (citable) accession number: Q8T8C0
Secondary accession number(s): Q9BLL0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families