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Q8T8C0

- NOS_BOMMO

UniProt

Q8T8C0 - NOS_BOMMO

Protein

Nitric oxide synthase

Gene

NOS

Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Involved in the induction of immune gene expression.1 PublicationCurated

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity

    Enzyme regulationi

    Expression is dependent on and stimulated by NADPH, calcium, BH4 and calmodulin. The activity is not dependent on FAD and is not stimulated by its presence.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi181 – 1811Iron (heme axial ligand)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi527 – 5315FMNPROSITE-ProRule annotation
    Nucleotide bindingi669 – 70032FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi811 – 82212FADBy similarityAdd
    BLAST
    Nucleotide bindingi954 – 96411FADBy similarityAdd
    BLAST
    Nucleotide bindingi1028 – 1147120NADPBy similarityAdd
    BLAST
    Nucleotide bindingi1128 – 114316NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. NADP binding Source: InterPro
    6. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitric oxide biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthaseImported (EC:1.14.13.39)
    Alternative name(s):
    Inducible nitric oxide synthase-like protein
    Gene namesi
    Name:NOSImported
    Synonyms:iNOS-LPImported
    OrganismiBombyx mori (Silk moth)
    Taxonomic identifieri7091 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
    ProteomesiUP000005204: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12091209Nitric oxide synthasePRO_0000403313Add
    BLAST

    Proteomic databases

    PRIDEiQ8T8C0.

    Expressioni

    Tissue specificityi

    Constitutively expressed at a low level in the larval fat body, hemocyte, Malpighian tubule, midgut, silk gland and adult antenna.1 Publication

    Inductioni

    Induced strongly in the fat body by lipopolysaccharide (LPS).1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8T8C0.
    SMRiQ8T8C0. Positions 63-482, 518-594.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini521 – 723203Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini776 – 1021246FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni491 – 51121Calmodulin-bindingBy similaritySequence AnalysisAdd
    BLAST
    Regioni516 – 53520Tetrahydrobiopterin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NOS family.Sequence Analysis
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 2 hits.
    3.90.340.10. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000333. NOS. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8T8C0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNFFKICCV KDPGGSAKEN LRKMDQVNGH FVSATCPFSG ESVELKVDSN     50
    QKQVKPNLRI KVPQPIRLKN HVAHDENFDT LHSRINEVTH FNTKCSEKVC 100
    QTSIMDIPGR GDTPRTAEEV FKDAQAFLTQ YYASIKRENS EAHKARLDEV 150
    KRELKERGTY QLKTSELVFG AKLAWRNATR CIGRIQWKKL QIFDCREVTT 200
    ASGMFEALCN HIKYATNKGN IRSAITIFPQ RTDGKHDYRI WNPQLINYAG 250
    YQEPDGSITG DPARVEFTEI CMKLGWKAPR TPWDILPLVL SADGKDPEFF 300
    ELPKEIVMEV QFEHPEYDWF KDMGWKWYAL PAVSNMRLAC GGLEFTANSF 350
    NGWYMGTEIG CRNLCDESRL NIVEAVAKKM GLDTNSFVSL WKDKALVEVN 400
    IAVLHSFHRD NVSIVDHHSA SEQFQKHLEN ENKSRGGCPA DWIWIVPPMS 450
    SSLTTVFHQE MALYYIRPSY DYQEPPWKTH QWTKSDGTKT VHRKFHFKQI 500
    ARAVKFTSKL FGRALSKRIK ATILYATETG KSEHYAKELG TIFGHAFNAQ 550
    VHCMSEYDMF SIEHETLVLI VASTFGNGEP PANGVDFAEH LFQMLYNETN 600
    NNRGDGTSDL GSGTFKTPTP KSLMRSNSMM TPSFEYKRQL TRLESNKSSV 650
    AGASSIEQIG PLSNVCFAVF GLGSSAYPKF CHFGKTVDKI LGDLGGERIL 700
    ELACGDELYG QEQQFRAWSS KIFQVACETF CLDENGMVKD AKKALGDVPL 750
    TEETVRFGKY QGDTKLKAAL EASFRKQLIT CKVKENKNLG DFSADRATVF 800
    VDMQPETEFK YDPGDHVGVL ACNRKEIVDA VLERMKDVDD YDKTVQLQVM 850
    KETLTPTGAI KTWEQHERLP AVTIRQIFTR FLDITTPPST TVLKYLSKAC 900
    TDQNDAAQLK ELAIDSNKYD DWRHLHYPHL AEVLAQFPSC RPQASLLAAL 950
    LPSLQPRFYS ISSSPLAHPH RIHITCAVVV YRTQNGQGPM HYGVCSTYLQ 1000
    NLKPEDEALV FIRRAPSFHM PKDLSAPLIL VGPGSGIAPF RGFWHHRKHQ 1050
    IKNLIPNKQK PGPVWLFFGC RNRGMDLYKE EKEKALADGV LSKVFLALSR 1100
    EDSVEKKHIQ TLLEDEGAEI SRMLLDENGH FYVCGDCKMA EEVQQKLKEI 1150
    MKKHAKMTEQ EYEEFILNLM DENRYHEDIF GITLRTAEVQ SASREFAKRT 1200
    RQESQKSQT 1209
    Length:1,209
    Mass (Da):137,507
    Last modified:June 1, 2002 - v1
    Checksum:i92A73F04B12C2E2C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti157 – 1571R → K in BAB33296. 1 PublicationCurated
    Sequence conflicti205 – 2051F → L in BAB33296. 1 PublicationCurated
    Sequence conflicti259 – 2591T → I in BAB33296. 1 PublicationCurated
    Sequence conflicti332 – 3321A → P in BAB33296. 1 PublicationCurated
    Sequence conflicti340 – 3412CG → KK in BAB33296. 1 PublicationCurated
    Sequence conflicti351 – 3522NG → KN in BAB33296. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB071182 mRNA. Translation: BAB85836.1.
    AB485775 mRNA. Translation: BAH23563.1.
    AB017521 mRNA. Translation: BAB33296.1.
    RefSeqiNP_001036963.1. NM_001043498.1.
    UniGeneiBmo.133.

    Genome annotation databases

    GeneIDi692510.
    KEGGibmor:692510.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB071182 mRNA. Translation: BAB85836.1 .
    AB485775 mRNA. Translation: BAH23563.1 .
    AB017521 mRNA. Translation: BAB33296.1 .
    RefSeqi NP_001036963.1. NM_001043498.1.
    UniGenei Bmo.133.

    3D structure databases

    ProteinModelPortali Q8T8C0.
    SMRi Q8T8C0. Positions 63-482, 518-594.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q8T8C0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 692510.
    KEGGi bmor:692510.

    Organism-specific databases

    CTDi 4842.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 2 hits.
    3.90.340.10. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000333. NOS. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning, characterization and gene expression of nitric oxide synthase from the silkworm, Bombyx mori."
      Imamura M., Yang J., Yamakawa M.
      Insect Mol. Biol. 11:257-265(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION.
      Strain: Tokai X Asahi1 Publication.
      Tissue: Larva1 Publication.
    2. "Identification of nitric-oxide synthase from Bombyx mori."
      Nagaoka S., Takata Y.
      Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Reproductive systemImported.
    3. "Inducible nitric oxide synthase-like protein(iNOS-LP) cDNA."
      Yang J., Taniai K., Yamakawa M.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-354.

    Entry informationi

    Entry nameiNOS_BOMMO
    AccessioniPrimary (citable) accession number: Q8T8C0
    Secondary accession number(s): Q9BLL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2011
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3