Q8T869 (BIP2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Luminal-binding protein 2
Short name=BiP 2

Gene names

Name:	bip2
ORF Names:	DDB_G0276445

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from direct assay PubMed 20422638. Source: dictyBase phagocytic vesicle Inferred from direct assay Ref.3PubMed 19482547. Source: dictyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 658	638	Luminal-binding protein 2		PRO_0000327978
Regions
	Motif	655 – 658	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	611	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8T869 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 5DE36C98EF85620A	FASTA	658	72,538
	10 20 30 40 50 60 MNKSIYALLC LLLLISFAAA AEKKEEEKKD YGSVIGIDLG TTYSCVGVFK KGKVEIIPND 70 80 90 100 110 120 QGNRITPSYV AFTETERLIG EAAKNQATLN PENTIFDIKR LIGRRFDDEE VQRDMKLLPY 130 140 150 160 170 180 KIVSKNNKPY VVVKVKGEEK TYSPEEISAM ILGRMKEIAE ASLGKTVTHA VVTCPAYFND 190 200 210 220 230 240 AQRAATKDAG VIAGLEVLRV INEPTAAALA YGFDATGDKE KNILVYDLGG GTFDVSVLSI 250 260 270 280 290 300 EDGVFEVRST NGDTHLGGED FDQRVMKHFL TVFQKKTGKD ASKDKKSLQK LRRAAENAKR 310 320 330 340 350 360 ILSTSPQTQL EIENFFDGKD LIETLTRAKF EELNMDLFKK TLDPVKKVLE DAKFKKSQIH 370 380 390 400 410 420 EVVLVGGSTR IPKIQQLLKD FFNGKEPNRG VHPDEAVAYG AAVQGGIFTN EEGTDTLVLL 430 440 450 460 470 480 DIAPLTLGIE TVGGVMTALI PRGTFVPTKK SQVFSTYQDN QDRVSIQIYE GERSMTKDNN 490 500 510 520 530 540 LLGKFDLSGI PPAQRGVPQI EVTFEMDVNG ILHVSAEDKA SGSKESITIT NDKERLSQAD 550 560 570 580 590 600 IDRMVKEAAD AADEDKAAKE RVEAKNTLEN YIYQIKNTIG DKDKIGGKIE SDDKETIETA 610 620 630 640 650 ISDALSWLDV NSSAEKEEFD EQYKILEKIV QPIFSKLYGA AGGSPNGAGE DMPNHDEL

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000015 Genomic DNA. Translation: EAL69176.1.
RefSeq	XP_643155.1. XM_638063.1.
3D structure databases
HSSP	HSSP built from PDB template 1HPM based on UniProtKB P19120.
ProteinModelPortal	Q8T869.
SMR	Q8T869. Positions 32-573.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0167089.
Proteomic databases
PRIDE	Q8T869.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233663; DDB0233663; DDB_G0276445.
GeneID	8620562.
KEGG	ddi:DDB_G0276445.
Organism-specific databases
dictyBase	DDB_G0276445.
Phylogenomic databases
eggNOG	COG0443.
KO	K09490.
OMA	ANAYCDE.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BIP2_DICDI

Accession

Primary (citable) accession number: Q8T869
Secondary accession number(s): Q551H9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)