Reviewed, UniProtKB/Swiss-Prot Q8T6B9 (PUF68_DROME)

Names and origin

Protein names

Recommended name:
    Poly(U)-binding-splicing factor half pint
      Short name=Protein half pint
Alternative name(s):
    68 kDa poly(U)-binding-splicing factor
    PUF60 homolog

Gene names

Name:	pUf68
Synonyms:	hfp
ORF Names:	CG12085

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Splicing factor that regulates oogenesis and controls both mitosis and mRNA localization in the germline by regulating mRNA splicing of a subset of genes within the ovary. Probably acts by regulating the alternative splice site selection of the otu transcript. Also regulates the alternative splicing of eIF-4E and grk, while it is not involved in the splicing of par-1, sqd and psq. Ref.2
Subunit structure	Interacts with enc. However, given the cytoplasmic localization of enc, the relevance of such interaction is unclear. Ref.2
Subcellular location	Nucleus. Ref.2
Tissue specificity	Expressed in all germline cells and within the follicle cell. Ref.2
Domain	The third RNA recognition motif, called PUMP domain in Ref.1, is atypical and may rather mediate protein-protein interactions. Ref.1
Sequence similarities	Belongs to the RRM half pint family. Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	Differentiation Oogenesis mRNA processing mRNA splicing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alternative nuclear mRNA splicing, via spliceosome Ref.2 Inferred from mutant phenotype. Source: FlyBase cystoblast division Ref.2 Inferred from mutant phenotype. Source: FlyBase mRNA splice site selection Ref.2 Inferred from mutant phenotype. Source: FlyBase multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oogenesis Inferred from electronic annotation. Source: UniProtKB-KW regulation of alternative nuclear mRNA splicing, via spliceosome Inferred from mutant phenotype. Source: FlyBase
Cellular component	nucleus Ref.2 Inferred from direct assay. Source: FlyBase
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VBK9	1	EBI-189756,EBI-98973
	Q9VCZ7	1	EBI-189756,EBI-99019
	Q9VIY9	1	EBI-189756,EBI-82519
	Q9VUR3	1	EBI-189756,EBI-96472

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q8T6B9-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q8T6B9-2)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	Poly(U)-binding-splicing factor half pint		PRO_0000081744
Regions
	Domain	130 – 208	79	RRM 1
	Domain	227 – 305	79	RRM 2
	Domain	537 – 627	91	RRM 3; atypical
	Compositional bias	322 – 479	158	Ala-rich
Amino acid modifications
	Modified residue	13	1	Phosphoserine Ref.6
	Modified residue	30	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 92	92	Missing in isoform B.		VSP_009328
Experimental info
	Sequence conflict	583	1	D → A in AAL86452. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (Long) [UniParc]. Last modified February 2, 2004. Version 2. Checksum: F0AD184D9EEF145F	FASTA	637	67,940
	10 20 30 40 50 60 MGSNDRASRS PRSDDQREIS DMPATKRTRS DSGKSTDSKI PYLSQPLYDL KQTGDVKFGP 70 80 90 100 110 120 GTRSALLGLL GGALPKLSSE QHDLVSKAKK YAMEQSIKMV LMKQTLAHQQ QQLATQRTQV 130 140 150 160 170 180 QRQQALALMC RVYVGSISFE LKEDTIRVAF TPFGPIKSIN MSWDPITQKH KGFAFVEYEI 190 200 210 220 230 240 PEGAQLALEQ MNGALMGGRN IKVGRPSNMP QAQQVIDEVQ EEAKSFNRIY VASIHPDLSE 250 260 270 280 290 300 EDIKSVFEAF GPILYCKLAQ GTSLHTHKGY GFIEYANKQA MDEAIASMNL FDLGGQLLRV 310 320 330 340 350 360 GRSITPPNAL ACPTTNSTMP TAAAVAAAAA TAKIQALDAV ASNAVLGLSQ NTPVMAAGAV 370 380 390 400 410 420 VTKVGAMPVV SAATSAAALH PALAQAAPAL LPPGIFQAPT PVAPSLLGVP AGLQPLQAVV 430 440 450 460 470 480 PTLPPPALLA TPTLPMTVGG VGVGLVPTVA TLAGAEASKG AAAAAALSAA ANNAAVTAAN 490 500 510 520 530 540 LSENIKKAHE KQQEELQKKL MDEGDVQTLQ QQENMSIKGQ SARQLVMQRL MRPVDSRVII 550 560 570 580 590 600 LRNMVGPEDV DETLQEEIQE ECSKFGTVSR VIIFNEKQTE NEDDDEAEII VKIFVEFSAG 610 620 630 AEAMRGKEAL DGRFFGGRRV VAELYDQGIF DQGDLSG
	Isoform B (C). Checksum: E75E378F7D07F24E	FASTA	545	57,989
	10 20 30 40 50 60 MEQSIKMVLM KQTLAHQQQQ LATQRTQVQR QQALALMCRV YVGSISFELK EDTIRVAFTP 70 80 90 100 110 120 FGPIKSINMS WDPITQKHKG FAFVEYEIPE GAQLALEQMN GALMGGRNIK VGRPSNMPQA 130 140 150 160 170 180 QQVIDEVQEE AKSFNRIYVA SIHPDLSEED IKSVFEAFGP ILYCKLAQGT SLHTHKGYGF 190 200 210 220 230 240 IEYANKQAMD EAIASMNLFD LGGQLLRVGR SITPPNALAC PTTNSTMPTA AAVAAAAATA 250 260 270 280 290 300 KIQALDAVAS NAVLGLSQNT PVMAAGAVVT KVGAMPVVSA ATSAAALHPA LAQAAPALLP 310 320 330 340 350 360 PGIFQAPTPV APSLLGVPAG LQPLQAVVPT LPPPALLATP TLPMTVGGVG VGLVPTVATL 370 380 390 400 410 420 AGAEASKGAA AAAALSAAAN NAAVTAANLS ENIKKAHEKQ QEELQKKLMD EGDVQTLQQQ 430 440 450 460 470 480 ENMSIKGQSA RQLVMQRLMR PVDSRVIILR NMVGPEDVDE TLQEEIQEEC SKFGTVSRVI 490 500 510 520 530 540 IFNEKQTENE DDDEAEIIVK IFVEFSAGAE AMRGKEALDG RFFGGRRVVA ELYDQGIFDQ GDLSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PUF60: a novel U2AF65-related splicing activity." Page-McCaw P.S., Amonlirdviman K., Sharp P.A. RNA 5:1548-1560(1999) [PubMed: 10606266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), DOMAIN.
[2]	"Half pint regulates alternative splice site selection in Drosophila." Van Buskirk C., Schuepbach T. Dev. Cell 2:343-353(2002) [PubMed: 11879639] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH ENC.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Head.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13 AND SER-30, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF190745 mRNA. Translation: AAF04132.1. AF479079 mRNA. Translation: AAL86452.1. AE014296 Genomic DNA. Translation: AAF47501.1. AE014296 Genomic DNA. Translation: AAG22221.1. AY061006 mRNA. Translation: AAL28554.1. BT003317 mRNA. Translation: AAO25077.1.
RefSeq	NP_525123.2. NP_728610.1.
UniGene	Dm.7331
3D structure databases
HSSP	HSSP built from PDB template 2U2F based on UniProtKB P26368.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8T6B9. 46 interactions.
Genome annotation databases
Ensembl	FBgn0028577. Drosophila melanogaster. [Contig view]
GeneID	38173.
KEGG	dme:Dmel_CG12085.
Organism-specific databases
FlyBase	FBgn0028577. pUf68.
Phylogenomic databases
HOGENOM	Q8T6B9.
OMA	Q8T6B9. IKSINMS.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-014647-MON.
Gene expression databases
ArrayExpress	Q8T6B9.
GermOnline	CG12085. Drosophila melanogaster.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR006532. PolyU_bd. IPR000504. RRM_RNP1. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 3 hits.
Pfam	PF00076. RRM_1. 2 hits. [Graphical view]
SMART	SM00360. RRM. 3 hits. [Graphical view]
TIGRFAMs	TIGR01645. half-pint. 1 hit.
PROSITE	PS50102. RRM. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	807323.

Entry information

Entry name

PUF68_DROME

Accession

Primary (citable) accession number: Q8T6B9
Secondary accession number(s): Q0E8J8 , Q95S11, Q9U696, Q9W0E6, Q9W0E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	February 2, 2004
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project