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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene
N/A
Organism
Plasmodium falciparum
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (ENO)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351NADCombined sources
Binding sitei80 – 801NADCombined sources
Binding sitei122 – 1221NADCombined sources
Active sitei153 – 1531NucleophileUniRule annotation
Sitei180 – 1801Activates thiol group during catalysisUniRule annotation
Binding sitei319 – 3191NADCombined sources
Binding sitei323 – 3231NADCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 144NADCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

NADUniRule annotationCombined sources, Nucleotide-bindingCombined sources

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation (EC:1.2.1.12UniRule annotation)
OrganismiPlasmodium falciparumImported
Taxonomic identifieri5833 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3559677.

PTM / Processingi

Proteomic databases

PaxDbiQ8T6B1.
PRIDEiQ8T6B1.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi5833.PF14_0598.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YWGX-ray2.60O/P/Q/R1-337[»]
2B4RX-ray2.25O/P/Q/R1-335[»]
2B4TX-ray2.50O/P/Q/R1-335[»]
ProteinModelPortaliQ8T6B1.
SMRiQ8T6B1. Positions 4-335.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8T6B1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 153150Gp_dh_NInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8T6B1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVTKLGING FGRIGRLVFR AAFGRKDIEV VAINDPFMDL NHLCYLLKYD
60 70 80 90 100
SVHGQFPCEV THADGFLLIG EKKVSVFAEK DPSQIPWGKC QVDVVCESTG
110 120 130 140 150
VFLTKELASS HLKGGAKKVI MSAPPKDDTP IYVMGINHHQ YDTKQLIVSN
160 170 180 190 200
ASCTTNCLAP LAKVINDRFG IVEGLMTTVH ASTANQLVVD GPSKGGKDWR
210 220 230 240 250
AGRCALSNII PASTGAAKAV GKVLPELNGK LTGVAFRVPI GTVSVVDLVC
260 270 280 290 300
RLQKPAKYEE VALEIKKAAE GPLKGILGYT EDEVVSQDFV HDNRSSIFDM
310 320 330
KAGLALNDNF FKLVSWYDNE WGYSNRVLDL AVHITNN
Length:337
Mass (Da):36,635
Last modified:June 1, 2002 - v1
Checksum:iC678D13F328F2BA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF479765 mRNA. Translation: AAL87686.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF479765 mRNA. Translation: AAL87686.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YWGX-ray2.60O/P/Q/R1-337[»]
2B4RX-ray2.25O/P/Q/R1-335[»]
2B4TX-ray2.50O/P/Q/R1-335[»]
ProteinModelPortaliQ8T6B1.
SMRiQ8T6B1. Positions 4-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5833.PF14_0598.

Chemistry

ChEMBLiCHEMBL3559677.

Proteomic databases

PaxDbiQ8T6B1.
PRIDEiQ8T6B1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Miscellaneous databases

EvolutionaryTraceiQ8T6B1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Bucher M.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum."
    Satchell J.F., Malby R.L., Luo C.S., Adisa A., Alpyurek A.E., Klonis N., Smith B.J., Tilley L., Colman P.M.
    Acta Crystallogr. D 61:1213-1221(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH NAD.
  3. "Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum at 2.25 A resolution reveals intriguing extra electron density in the active site."
    Robien M.A., Bosch J., Buckner F.S., Van Voorhis W.C., Worthey E.A., Myler P., Mehlin C., Boni E.E., Kalyuzhniy O., Anderson L., Lauricella A., Gulde S., Luft J.R., DeTitta G., Caruthers J.M., Hodgson K.O., Soltis M., Zucker F.
    , Verlinde C.L., Merritt E.A., Schoenfeld L.W., Hol W.G.
    Proteins 62:570-577(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1-335 IN COMPLEX WITH NAD.

Entry informationi

Entry nameiQ8T6B1_PLAFA
AccessioniPrimary (citable) accession number: Q8T6B1
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2002
Last sequence update: June 1, 2002
Last modified: June 8, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.