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Protein

Na(+)/H(+) exchanger protein 2

Gene

nhx-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Na+/H+ antiporter that promotes normal di- or tripeptide transporter function, recovery following the peptide-induced acidification of the intestinal cytoplasm and maintenance of the peptide-dependent intestinal pH homeostasis. Regulator of free fatty acid uptake from the diet together with the dipeptide transporter pept-1. May play a timekeeper role in defecation cycle but is not necessary for pbo-4-dependent proton release. May play a role in the regulation of lifespan independent of the stress response pathway.2 Publications

GO - Molecular functioni

  • sodium:proton antiporter activity Source: WormBase

GO - Biological processi

  • determination of adult lifespan Source: WormBase
  • lipid storage Source: WormBase
  • nematode larval development Source: WormBase
  • positive regulation of fatty acid transport Source: WormBase
  • positive regulation of growth rate Source: WormBase
  • positive regulation of multicellular organism growth Source: WormBase
  • regulation of peptide transport Source: WormBase
  • regulation of pH Source: WormBase
  • regulation of pharyngeal pumping Source: WormBase
  • reproduction Source: WormBase
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-CEL-2160916. Hyaluronan uptake and degradation.
R-CEL-425986. Sodium/Proton exchangers.

Protein family/group databases

TCDBi2.A.36.1.8. the monovalent cation:proton antiporter-1 (cpa1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) exchanger protein 21 Publication
Alternative name(s):
Na(+)/H(+) antiporter nhx-21 Publication
Gene namesi
Name:nhx-2Imported
ORF Names:B0495.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiB0495.4; CE48832; WBGene00003730; nhx-2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Intramembranei7 – 2721HelicalSequence analysisAdd
BLAST
Topological domaini28 – 5124CytoplasmicSequence analysisAdd
BLAST
Intramembranei52 – 7221HelicalSequence analysisAdd
BLAST
Topological domaini73 – 786CytoplasmicSequence analysis
Transmembranei79 – 9921HelicalSequence analysisAdd
BLAST
Topological domaini100 – 1078ExtracellularSequence analysis
Transmembranei108 – 12821HelicalSequence analysisAdd
BLAST
Topological domaini129 – 1379CytoplasmicSequence analysis
Transmembranei138 – 15821HelicalSequence analysisAdd
BLAST
Topological domaini159 – 17315ExtracellularSequence analysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence analysisAdd
BLAST
Topological domaini195 – 2017CytoplasmicSequence analysis
Transmembranei202 – 22221HelicalSequence analysisAdd
BLAST
Topological domaini223 – 24422ExtracellularSequence analysisAdd
BLAST
Transmembranei245 – 26521HelicalSequence analysisAdd
BLAST
Topological domaini266 – 29429CytoplasmicSequence analysisAdd
BLAST
Transmembranei295 – 31521HelicalSequence analysisAdd
BLAST
Topological domaini316 – 33318ExtracellularSequence analysisAdd
BLAST
Transmembranei334 – 35421HelicalSequence analysisAdd
BLAST
Topological domaini355 – 36410CytoplasmicSequence analysis
Transmembranei365 – 38521HelicalSequence analysisAdd
BLAST
Topological domaini386 – 40116ExtracellularSequence analysisAdd
BLAST
Intramembranei402 – 42221HelicalSequence analysisAdd
BLAST
Topological domaini423 – 4297ExtracellularSequence analysis
Transmembranei430 – 45021HelicalSequence analysisAdd
BLAST
Topological domaini451 – 644194CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: WormBase
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown of the protein causes worms to become extremely lean and transparent. Slower progression through the larval stages, in particular the L1 and L2 stages. Increase in average life span, decreased brood size and loss of fat storage granules in the intestine leading to decreased opacity. Reduced rate of pharyngeal pumping, reduced size of proton pulses inside the cells, increased intracellular acidification, reduced uptake of fatty acids, and doubling of the defecation period.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 644644Na(+)/H(+) exchanger protein 2PRO_0000424423Add
BLAST

Proteomic databases

PaxDbiQ8T5S1.

Expressioni

Tissue specificityi

Expressed uniformly in the intestinal epithelial cells.2 Publications

Developmental stagei

Biphasic expression pattern with high expression in the L1 and L2 larval stages and gradual decrease through adult stages.1 Publication

Gene expression databases

BgeeiWBGene00003730.

Interactioni

Protein-protein interaction databases

STRINGi6239.B0495.4.

Structurei

3D structure databases

ProteinModelPortaliQ8T5S1.
SMRiQ8T5S1. Positions 176-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1966. Eukaryota.
COG0025. LUCA.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000247044.
OrthoDBiEOG091G02Q0.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8T5S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLRRPWVL VVGLLLIMSY VGAECEEEEK EYPSRYPIAY FEWENVKIPM
60 70 80 90 100
TICLWLIGAS IAKIIFNLIP HLNELFPDSA LLIMIGLIIG IIFKLIGVNK
110 120 130 140 150
NAFFLESEVF MLYLLPPLVF DAGYFMPARQ FFDNFGSILC FAMIGTSFNI
160 170 180 190 200
VAIALSLWAI SLTGLFSVET PLMHMLLFGS VAADVDPVAV IVIFEELKVN
210 220 230 240 250
EVLFIAVFGE SLLNDGVAVV LYRMFLTFSE IGTENLITSD YINGGVSFLV
260 270 280 290 300
VAFGGIGIGL LFAFLTSLVT RFARDEEVKV LNSVFILILP YTCYLCGELF
310 320 330 340 350
GLSSIMAIVF CGAAMRQYCR ENVDPDTVKA TESFIKVLSL ASETVIFVFL
360 370 380 390 400
GLSTVSSNHH WDTSFIVLTV VFCLIYRTLG VVVMCYFLNK YRLNKYTKVD
410 420 430 440 450
QFIMAYGGLR GAIAYGLVVA IPDFIPGKNM FVTSCIIVIY FTVFLQGITL
460 470 480 490 500
KPIAEFLQVE KKNVHSKNMI EHIYSELIDT TMAGMEDIAG FKGHHWIRDS
510 520 530 540 550
WNALNNNYLR PILVNKNNMK EMDKTKLVRK YKHLVDEDAK KIARGDLNSN
560 570 580 590 600
MVFTKALIEH TRSRTNTMID GVSSTSKIDF TKHMKENFGV TVYDDHSTVP
610 620 630 640
MTPTHLFQET TEVEYSVRSE INDNDGFEND GYESDESGSF HERV
Length:644
Mass (Da):72,611
Last modified:June 1, 2002 - v1
Checksum:iBB32044A0E39F6A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF497824 mRNA. Translation: AAM18102.1.
FO080132 Genomic DNA. Translation: CCD61472.2.
PIRiH88215.
RefSeqiNP_495614.4. NM_063213.5.
UniGeneiCel.9292.

Genome annotation databases

EnsemblMetazoaiB0495.4; B0495.4; WBGene00003730.
GeneIDi174242.
KEGGicel:CELE_B0495.4.
UCSCiB0495.4. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF497824 mRNA. Translation: AAM18102.1.
FO080132 Genomic DNA. Translation: CCD61472.2.
PIRiH88215.
RefSeqiNP_495614.4. NM_063213.5.
UniGeneiCel.9292.

3D structure databases

ProteinModelPortaliQ8T5S1.
SMRiQ8T5S1. Positions 176-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.B0495.4.

Protein family/group databases

TCDBi2.A.36.1.8. the monovalent cation:proton antiporter-1 (cpa1) family.

Proteomic databases

PaxDbiQ8T5S1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0495.4; B0495.4; WBGene00003730.
GeneIDi174242.
KEGGicel:CELE_B0495.4.
UCSCiB0495.4. c. elegans.

Organism-specific databases

CTDi174242.
WormBaseiB0495.4; CE48832; WBGene00003730; nhx-2.

Phylogenomic databases

eggNOGiKOG1966. Eukaryota.
COG0025. LUCA.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000247044.
OrthoDBiEOG091G02Q0.

Enzyme and pathway databases

ReactomeiR-CEL-2160916. Hyaluronan uptake and degradation.
R-CEL-425986. Sodium/Proton exchangers.

Miscellaneous databases

PROiQ8T5S1.

Gene expression databases

BgeeiWBGene00003730.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNHX2_CAEEL
AccessioniPrimary (citable) accession number: Q8T5S1
Secondary accession number(s): Q09432
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.