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Protein

Pupal cuticle protein 27

Gene

PCP27

Organism
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the pupal abdominal endocuticle. May have important role in the pharate pupal cuticle structure.1 Publication1 Publication

GO - Molecular functioni

Names & Taxonomyi

Protein namesi
Recommended name:
Pupal cuticle protein 27
Alternative name(s):
MS-PCP27
Short name:
MsCP27
Gene namesi
Name:PCP27
OrganismiManduca sexta (Tobacco hawkmoth) (Tobacco hornworm)Imported
Taxonomic identifieri7130 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaLepidopteraGlossataDitrysiaBombycoideaSphingidaeSphinginaeSphinginiManduca

Subcellular locationi

Keywords - Cellular componenti

Cuticle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 152 PublicationsAdd BLAST15
ChainiPRO_000000641116 – 180Pupal cuticle protein 271 PublicationAdd BLAST165

Expressioni

Developmental stagei

Expression is increased to a peak level just before pupal ecdysis.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 129Chitin-binding type R&RPROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000219175.

Family and domain databases

InterProiView protein in InterPro
IPR000618. Insect_cuticle.
PfamiView protein in Pfam
PF00379. Chitin_bind_4. 1 hit.
PRINTSiPR00947. CUTICLE.
PROSITEiView protein in PROSITE
PS51155. CHIT_BIND_RR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8T4J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLIIYVTII ALTSAAELPS RNYIPQDQGS GPYPGPHGGI GGDTGNRRPQ
60 70 80 90 100
QDAEKNSNVV KQEQEISDSG NYHFGFETSN GIRAEEAGGP EQAQGGYSYK
110 120 130 140 150
GDDGQTYTLI YTSGEGGFKP QGEHLPVAPP TPEAILIALQ QNERDEAAGI
160 170 180
FDDGQYHPEN NGQSRPGASA GAGTGSGYHY
Length:180
Mass (Da):19,028
Last modified:June 1, 2002 - v1
Checksum:i9F39E103FC1C6B5B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30S → P AA sequence (PubMed:12609518).Curated1
Sequence conflicti39G → S AA sequence (PubMed:10646967).Curated1
Sequence conflicti164S → N AA sequence (PubMed:12609518).Curated1

Mass spectrometryi

Molecular mass is 17623 Da from positions 16 - 180. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY083171 mRNA. Translation: AAL92478.1.

Similar proteinsi

Entry informationi

Entry nameiCU27_MANSE
AccessioniPrimary (citable) accession number: Q8T4J9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: June 1, 2002
Last modified: April 12, 2017
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing