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Protein

Protein enabled

Gene

ena

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions, together with Abl, trio and fra, in a complex signaling network that regulates axon guidance at the CNS midline. Required in part for robo-mediated repulsive axon guidance. May be involved in lamellipodial dynamics.1 Publication

GO - Molecular functioni

  • actin binding Source: FlyBase
  • SH3 domain binding Source: UniProtKB

GO - Biological processi

  • actin cytoskeleton organization Source: FlyBase
  • actin filament organization Source: FlyBase
  • axon guidance Source: FlyBase
  • axonogenesis Source: FlyBase
  • border follicle cell migration Source: FlyBase
  • compound eye development Source: FlyBase
  • cytoplasmic transport, nurse cell to oocyte Source: FlyBase
  • cytoskeleton organization Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • dorsal closure Source: FlyBase
  • epithelial cell morphogenesis Source: FlyBase
  • filopodium assembly Source: FlyBase
  • formation of a compartment boundary Source: FlyBase
  • germ-band shortening Source: FlyBase
  • gonad morphogenesis Source: FlyBase
  • head involution Source: FlyBase
  • negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • positive regulation of cell migration Source: FlyBase
  • positive regulation of cell projection organization Source: FlyBase
  • positive regulation of filopodium assembly Source: FlyBase
  • regulation of actin cytoskeleton organization Source: FlyBase
  • regulation of actin polymerization or depolymerization Source: FlyBase
  • regulation of cell shape Source: FlyBase
  • regulation of filopodium assembly Source: FlyBase
  • regulation of lamellipodium assembly Source: FlyBase
  • subsynaptic reticulum organization Source: FlyBase
  • suture of dorsal opening Source: FlyBase
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-DME-376176. Signaling by Robo receptor.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein enabled
Gene namesi
Name:ena
Synonyms:enb
ORF Names:CG15112
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0000578. ena.

Subcellular locationi

GO - Cellular componenti

  • adherens junction Source: FlyBase
  • axon Source: FlyBase
  • cell-cell junction Source: FlyBase
  • cell leading edge Source: FlyBase
  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • dendrite Source: FlyBase
  • filopodium tip Source: FlyBase
  • lamellipodium Source: UniProtKB
  • subsynaptic reticulum Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002277571 – 980Protein enabledAdd BLAST980

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei425Phosphotyrosine1 Publication1
Modified residuei607Phosphotyrosine1 Publication1
Modified residuei625Phosphotyrosine1 Publication1
Modified residuei650Phosphotyrosine1 Publication1
Modified residuei666Phosphotyrosine1 Publication1
Modified residuei826Phosphotyrosine1 Publication1
Modified residuei905Phosphoserine1 Publication1
Modified residuei914Phosphoserine1 Publication1
Modified residuei924Phosphoserine2 Publications1

Post-translational modificationi

Tyrosine phosphorylated on multiple sites by Abl kinase. In vitro, phosphorylation on specific tyrosine residues inhibits interaction with Abl and Src SH3 domains.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8T4F7.
PRIDEiQ8T4F7.

PTM databases

iPTMnetiQ8T4F7.

Expressioni

Tissue specificityi

Expressed in axons of the embryonic nervous system.1 Publication

Gene expression databases

BgeeiFBgn0000578.
ExpressionAtlasiQ8T4F7. baseline.
GenevisibleiQ8T4F7. DM.

Interactioni

Subunit structurei

Interacts with Abl and Src SH3 domains. Binds, in vitro and in vivo, the cytoplasmic domain of robo. Interacts with Zyx102EF and chic.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AblP005222EBI-466810,EBI-534090
chicP258433EBI-466810,EBI-156199
LarP166212EBI-466810,EBI-668630
SrcP054802EBI-466810,EBI-298680From a different organism.

GO - Molecular functioni

  • actin binding Source: FlyBase
  • SH3 domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi62866. 44 interactors.
IntActiQ8T4F7. 33 interactors.
MINTiMINT-817415.
STRINGi7227.FBpp0113115.

Structurei

3D structure databases

ProteinModelPortaliQ8T4F7.
SMRiQ8T4F7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 407WH1PROSITE-ProRule annotationAdd BLAST262

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni791 – 980EVH2Add BLAST190
Regioni791 – 810EVH2 block AAdd BLAST20
Regioni837 – 854EVH2 block BAdd BLAST18
Regioni947 – 980EVH2 block CAdd BLAST34

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi800 – 803KLKR4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi157 – 220Ser-richAdd BLAST64
Compositional biasi244 – 279Gln-richAdd BLAST36
Compositional biasi457 – 685Gln-richAdd BLAST229
Compositional biasi633 – 784Pro-richAdd BLAST152

Domaini

The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

Sequence similaritiesi

Belongs to the Ena/VASP family.Curated
Contains 1 WH1 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG4590. Eukaryota.
ENOG41101TS. LUCA.
GeneTreeiENSGT00730000110272.
InParanoidiQ8T4F7.
OMAiMVGHGHL.
OrthoDBiEOG091G0QTE.
PhylomeDBiQ8T4F7.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR014885. VASP_tetra.
IPR000697. WH1/EVH1_dom.
[Graphical view]
PfamiPF08776. VASP_tetra. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
SMARTiSM00461. WH1. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50229. WH1. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q8T4F7-4) [UniParc]FASTAAdd to basket
Also known as: F

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMKKLYAKT SFTSKKPSSA ANSTPILAYH QQQHQQPGNG ICEFQVVAPG
60 70 80 90 100
HSGELMIRRS QSMHHKMSPP VGGLGSKSEY YSIEELQELD LLDYRHPMYH
110 120 130 140 150
HYQQQELRQR YHEHEQLVLQ LPKATSPKAG PIYEAPQRSQ QQQDQMLYVP
160 170 180 190 200
TAAQRDSSSS AAATSIASSS TLTSSPSPSS SSSLIFSTLR KCVSPSNPSV
210 220 230 240 250
NPNQPSKTQP SKLGCSMSFS IRTTTATAAT AAAANAATAT LSTQQQQQQA
260 270 280 290 300
QQQHKQHLYS NIHHYLIRQQ QQKQHYTLQR RHNSVKDKFI GGITTIFAEQ
310 320 330 340 350
SIIGARASVM VYDDNQKKWV PSGSSSGLSK VQIYHHQQNN TFRVVGRKLQ
360 370 380 390 400
DHEVVINCSI LKGLKYNQAT ATFHQWRDSK FVYGLNFSSQ NDAENFARAM
410 420 430 440 450
MHALEVLSGR VANNPGGPPT NGNGYEEDMG YRTMTSEDAA ILRQNNSIGG
460 470 480 490 500
HVTPSAQTPT SQTNQNNIPQ SPPTPQGHHR TSSAPPAPQP QQQQQQQQAQ
510 520 530 540 550
QMGQPGSHYG PTGNGPTSNG LPQQVNSQIP PAPQQQPQQQ QFQQQQQQQQ
560 570 580 590 600
YQQMVQAGYA PSQQYQQPHY VLSNSNPNLT VHQYPTQQAQ QQPPQAPQPP
610 620 630 640 650
LQNGGMYMVG HGHLPSSASA NSVVYASQQQ MLPQAHPQAP QAPTMPGPGY
660 670 680 690 700
GGPPVPPPQQ QAENPYGQVP MPPPMNPSQQ QQPGQVPLNR MSSQGGPGGP
710 720 730 740 750
PAPAPPPPPP SFGGAAGGGP PPPAPPQMFN GAPPPPAMGG GPPPAPPAPP
760 770 780 790 800
AMGGGPPPAP GGPGAPPPPP PPPGLGGAPK KEDPQADLMG SLASQLQQIK
810 820 830 840 850
LKKNKVTTSA PENSGSSTSS GGSGNYGTIG RSSNGMASMM DEMAKTLARR
860 870 880 890 900
RAQAEKKDPD PEAEVKKRPW EKSNTLPHKL SGGAGSGSAG SGHEGANGNS
910 920 930 940 950
GGAGSNTTNS GGESPRPMRK RFGSASEETI LKVNGDGLSL ALSNGDLDTL
960 970 980
KAEIVREMRL EIQKVKNEII DAIKSEFNRR
Note: No experimental confirmation available.
Length:980
Mass (Da):104,824
Last modified:July 7, 2009 - v4
Checksum:i7B084A0AEC098297
GO
Isoform 1 (identifier: Q8T4F7-1) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: L → F
     148-298: Missing.
     482-482: S → SRNSL
     932-932: K → KQ

Note: No experimental confirmation available.
Show »
Length:834
Mass (Da):89,280
Checksum:i7E0CD375D351C674
GO
Isoform 2 (identifier: Q8T4F7-2) [UniParc]FASTAAdd to basket
Also known as: A, C, E

The sequence of this isoform differs from the canonical sequence as follows:
     2-297: Missing.
     298-298: A → T

Show »
Length:684
Mass (Da):71,985
Checksum:iE1FFB5A8091FA77E
GO
Isoform 3 (identifier: Q8T4F7-3) [UniParc]FASTAAdd to basket
Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     2-297: Missing.
     298-298: A → T
     482-482: S → SRNSL
     563-563: Missing.

Note: No experimental confirmation available.
Show »
Length:687
Mass (Da):72,327
Checksum:iF480275EB4CC79A8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti120Q → H in AAL89948 (PubMed:12537569).Curated1
Sequence conflicti491 – 493Missing in AAL89948 (PubMed:12537569).Curated3
Sequence conflicti612G → S in AAA85120 (PubMed:7535279).Curated1
Sequence conflicti799I → F in AAA85120 (PubMed:7535279).Curated1
Sequence conflicti799I → F in AAL89948 (PubMed:12537569).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0175742 – 297Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST296
Alternative sequenceiVSP_037651147L → F in isoform 1. 1 Publication1
Alternative sequenceiVSP_037652148 – 298Missing in isoform 1. 1 PublicationAdd BLAST151
Alternative sequenceiVSP_017575298A → T in isoform 2 and isoform 3. 1 Publication1
Alternative sequenceiVSP_019865482S → SRNSL in isoform 1 and isoform 3. 2 Publications1
Alternative sequenceiVSP_019866563Missing in isoform 3. 1 Publication1
Alternative sequenceiVSP_037653932K → KQ in isoform 1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21123 Genomic DNA. Translation: AAA85120.1.
AE013599 Genomic DNA. Translation: AAF57598.2.
AE013599 Genomic DNA. Translation: AAM68438.2.
AE013599 Genomic DNA. Translation: AAM68439.2.
AE013599 Genomic DNA. Translation: AAX52696.1.
AE013599 Genomic DNA. Translation: AAX52697.1.
AE013599 Genomic DNA. Translation: ACL83163.1.
AY084210 mRNA. Translation: AAL89948.1.
BT004488 mRNA. Translation: AAO42652.1.
PIRiA56154.
RefSeqiNP_001014536.1. NM_001014536.2. [Q8T4F7-2]
NP_001014537.1. NM_001014537.3. [Q8T4F7-3]
NP_001137709.1. NM_001144237.2. [Q8T4F7-4]
NP_725857.1. NM_166329.2. [Q8T4F7-2]
NP_725858.1. NM_166330.2. [Q8T4F7-2]
NP_725859.2. NM_166331.3. [Q8T4F7-1]
UniGeneiDm.14700.

Genome annotation databases

EnsemblMetazoaiFBtr0114623; FBpp0113115; FBgn0000578. [Q8T4F7-4]
GeneIDi37201.
KEGGidme:Dmel_CG15112.
UCSCiCG15112-RA. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21123 Genomic DNA. Translation: AAA85120.1.
AE013599 Genomic DNA. Translation: AAF57598.2.
AE013599 Genomic DNA. Translation: AAM68438.2.
AE013599 Genomic DNA. Translation: AAM68439.2.
AE013599 Genomic DNA. Translation: AAX52696.1.
AE013599 Genomic DNA. Translation: AAX52697.1.
AE013599 Genomic DNA. Translation: ACL83163.1.
AY084210 mRNA. Translation: AAL89948.1.
BT004488 mRNA. Translation: AAO42652.1.
PIRiA56154.
RefSeqiNP_001014536.1. NM_001014536.2. [Q8T4F7-2]
NP_001014537.1. NM_001014537.3. [Q8T4F7-3]
NP_001137709.1. NM_001144237.2. [Q8T4F7-4]
NP_725857.1. NM_166329.2. [Q8T4F7-2]
NP_725858.1. NM_166330.2. [Q8T4F7-2]
NP_725859.2. NM_166331.3. [Q8T4F7-1]
UniGeneiDm.14700.

3D structure databases

ProteinModelPortaliQ8T4F7.
SMRiQ8T4F7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62866. 44 interactors.
IntActiQ8T4F7. 33 interactors.
MINTiMINT-817415.
STRINGi7227.FBpp0113115.

PTM databases

iPTMnetiQ8T4F7.

Proteomic databases

PaxDbiQ8T4F7.
PRIDEiQ8T4F7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0114623; FBpp0113115; FBgn0000578. [Q8T4F7-4]
GeneIDi37201.
KEGGidme:Dmel_CG15112.
UCSCiCG15112-RA. d. melanogaster.

Organism-specific databases

CTDi37201.
FlyBaseiFBgn0000578. ena.

Phylogenomic databases

eggNOGiKOG4590. Eukaryota.
ENOG41101TS. LUCA.
GeneTreeiENSGT00730000110272.
InParanoidiQ8T4F7.
OMAiMVGHGHL.
OrthoDBiEOG091G0QTE.
PhylomeDBiQ8T4F7.

Enzyme and pathway databases

ReactomeiR-DME-376176. Signaling by Robo receptor.

Miscellaneous databases

ChiTaRSiena. fly.
GenomeRNAii37201.
PROiQ8T4F7.

Gene expression databases

BgeeiFBgn0000578.
ExpressionAtlasiQ8T4F7. baseline.
GenevisibleiQ8T4F7. DM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR014885. VASP_tetra.
IPR000697. WH1/EVH1_dom.
[Graphical view]
PfamiPF08776. VASP_tetra. 1 hit.
PF00568. WH1. 1 hit.
[Graphical view]
SMARTiSM00461. WH1. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50229. WH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENA_DROME
AccessioniPrimary (citable) accession number: Q8T4F7
Secondary accession number(s): A4UZP3
, B7YZL0, Q24035, Q86NN6, Q8MMB3, Q9V8R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 7, 2009
Last modified: November 30, 2016
This is version 128 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.