Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8T390

- SH3G3_DROME

UniProt

Q8T390 - SH3G3_DROME

Protein

Endophilin-A

Gene

endoA

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis.2 Publications

    GO - Molecular functioni

    1. lysophosphatidic acid acyltransferase activity Source: FlyBase
    2. phospholipid binding Source: FlyBase

    GO - Biological processi

    1. membrane tubulation Source: FlyBase
    2. metabolic process Source: GOC
    3. neurotransmitter secretion Source: FlyBase
    4. regulation of synapse structure and activity Source: FlyBase
    5. synaptic vesicle endocytosis Source: FlyBase

    Keywords - Biological processi

    Endocytosis

    Enzyme and pathway databases

    SignaLinkiQ8T390.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endophilin-A
    Alternative name(s):
    SH3 domain-containing GRB2-like protein
    Gene namesi
    Name:endoAImported
    Synonyms:endo1 Publication
    ORF Names:CG14296
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0038659. endoA.

    Subcellular locationi

    Cytoplasm 2 Publications. Membrane 2 Publications; Peripheral membrane protein 2 Publications
    Note: Associated with internal membranes. Expressed presynaptically at NMJs.2 Publications

    GO - Cellular componenti

    1. cytoplasm Source: FlyBase
    2. cytosol Source: FlyBase
    3. membrane Source: FlyBase

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Flies exhibit dramatic impairment of clathrin-mediated endocytosis. Total loss of endoA causes severe defects in motility and death at the early L2 larval stage.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Endophilin-APRO_0000285838Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei285 – 2851Phosphoserine1 Publication
    Modified residuei289 – 2891Phosphoserine1 Publication
    Modified residuei293 – 2931Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8T390.
    PRIDEiQ8T390.

    Expressioni

    Tissue specificityi

    Expression is abundant in central nervous system (brain lobes and the ventral nerve cord) starting at embryonic stage 13 through to third instar larvae. Also expressed in photoreceptors in the eye imaginal disks and the Bolwig organs of the peripheral nervous system.2 Publications

    Gene expression databases

    BgeeiQ8T390.

    Interactioni

    Protein-protein interaction databases

    BioGridi67269. 5 interactions.
    IntActiQ8T390. 6 interactions.
    MINTiMINT-880691.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8T390.
    SMRiQ8T390. Positions 30-251, 307-366.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 248231BARPROSITE-ProRule annotationAdd
    BLAST
    Domaini305 – 36460SH3PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili227 – 24721Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the endophilin family.Sequence Analysis
    Contains 1 BAR domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG307129.
    GeneTreeiENSGT00550000074464.
    InParanoidiQ8T390.
    KOiK11247.
    OMAiENDTEHV.
    OrthoDBiEOG7G1V6H.
    PhylomeDBiQ8T390.

    Family and domain databases

    Gene3Di1.20.1270.60. 1 hit.
    InterProiIPR027267. AH/BAR-dom.
    IPR004148. BAR_dom.
    IPR028501. Endophilin-A.
    IPR001452. SH3_domain.
    IPR013315. Spectrin_alpha_SH3.
    [Graphical view]
    PANTHERiPTHR10661:SF120. PTHR10661:SF120. 1 hit.
    PfamiPF03114. BAR. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    PR01887. SPECTRNALPHA.
    SMARTiSM00721. BAR. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS51021. BAR. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8T390-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFAGLKKQI NKANQYMTEK MGGAEGTKLD MDFMEMERKT DVTVELVEEL    50
    QLKTKEFLQP NPTARAKMAA VKGISKLSGQ AKSNTYPQPE GLLAECMLTY 100
    GKKLGEDNSV FAQALVEFGE ALKQMADVKY SLDDNIKQNF LEPLHHMQTK 150
    DLKEVMHHRK KLQGRRLDFD CKRRRQAKDD EIRGAEDKFG ESLQLAQVGM 200
    FNLLENDTEH VSQLVTFAEA LYDFHSQCAD VLRGLQETLQ EKRSEAESRP 250
    RNEFVPKTLL DLNLDGGGGG LNEDGTPSHI SSSASPLPSP MRSPAKSMAV 300
    TPQRQQQPCC QALYDFEPEN PGELAFKEND IITLLNRVDD NWFEGAVNGR 350
    TGYFPQSYVQ VQVPLPNGN 369
    Length:369
    Mass (Da):41,409
    Last modified:June 1, 2002 - v1
    Checksum:iF6B82FAEA6FF31A9
    GO

    RNA editingi

    Partially edited. Target of Adar.1 Publication

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti129 – 1291K → R in RNA edited version. 2 Publications
    Natural varianti137 – 1371K → E in RNA edited version. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF426170 mRNA. Translation: AAL24818.1.
    AJ437141 mRNA. Translation: CAD24682.1.
    AE014297 Genomic DNA. Translation: AAF55606.2.
    BT003300 mRNA. Translation: AAO25060.1.
    RefSeqiNP_001262717.1. NM_001275788.1.
    NP_620122.2. NM_138767.3.
    NP_732383.1. NM_169838.2.
    UniGeneiDm.3503.

    Genome annotation databases

    EnsemblMetazoaiFBtr0083698; FBpp0083112; FBgn0038659.
    FBtr0083699; FBpp0083113; FBgn0038659.
    GeneIDi42265.
    KEGGidme:Dmel_CG14296.

    Keywords - Coding sequence diversityi

    RNA editing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF426170 mRNA. Translation: AAL24818.1 .
    AJ437141 mRNA. Translation: CAD24682.1 .
    AE014297 Genomic DNA. Translation: AAF55606.2 .
    BT003300 mRNA. Translation: AAO25060.1 .
    RefSeqi NP_001262717.1. NM_001275788.1.
    NP_620122.2. NM_138767.3.
    NP_732383.1. NM_169838.2.
    UniGenei Dm.3503.

    3D structure databases

    ProteinModelPortali Q8T390.
    SMRi Q8T390. Positions 30-251, 307-366.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 67269. 5 interactions.
    IntActi Q8T390. 6 interactions.
    MINTi MINT-880691.

    Proteomic databases

    PaxDbi Q8T390.
    PRIDEi Q8T390.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0083698 ; FBpp0083112 ; FBgn0038659 .
    FBtr0083699 ; FBpp0083113 ; FBgn0038659 .
    GeneIDi 42265.
    KEGGi dme:Dmel_CG14296.

    Organism-specific databases

    CTDi 42265.
    FlyBasei FBgn0038659. endoA.

    Phylogenomic databases

    eggNOGi NOG307129.
    GeneTreei ENSGT00550000074464.
    InParanoidi Q8T390.
    KOi K11247.
    OMAi ENDTEHV.
    OrthoDBi EOG7G1V6H.
    PhylomeDBi Q8T390.

    Enzyme and pathway databases

    SignaLinki Q8T390.

    Miscellaneous databases

    GenomeRNAii 42265.
    NextBioi 827957.

    Gene expression databases

    Bgeei Q8T390.

    Family and domain databases

    Gene3Di 1.20.1270.60. 1 hit.
    InterProi IPR027267. AH/BAR-dom.
    IPR004148. BAR_dom.
    IPR028501. Endophilin-A.
    IPR001452. SH3_domain.
    IPR013315. Spectrin_alpha_SH3.
    [Graphical view ]
    PANTHERi PTHR10661:SF120. PTHR10661:SF120. 1 hit.
    Pfami PF03114. BAR. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    PR01887. SPECTRNALPHA.
    SMARTi SM00721. BAR. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS51021. BAR. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release."
      Verstreken P., Kjaerulff O., Lloyd T.E., Atkinson R., Zhou Y., Meinertzhagen I.A., Bellen H.J.
      Cell 109:101-112(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, RNA EDITING OF POSITION 129, DISRUPTION PHENOTYPE.
      Strain: Berkeley1 Publication.
      Tissue: Head1 Publication.
    2. "Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junction."
      Guichet A., Wucherpfennig T., Dudu V., Etter S., Wilsch-Brauniger M., Hellwig A., Gonzalez-Gaitan M., Huttner W.B., Schmidt A.A.
      EMBO J. 21:1661-1672(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Berkeley1 Publication.
      Tissue: HeadImported.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley1 Publication.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], RNA EDITING OF POSITION 129.
      Strain: BerkeleyImported.
      Tissue: Head.
    6. "RNA editing in Drosophila melanogaster: new targets and functional consequences."
      Stapleton M., Carlson J.W., Celniker S.E.
      RNA 12:1922-1932(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA EDITING OF POSITIONS 129 AND 137.
    7. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-285; SER-289 AND SER-293, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiSH3G3_DROME
    AccessioniPrimary (citable) accession number: Q8T390
    Secondary accession number(s): Q95V65, Q9VE25
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3