Q8T390 (SH3G3_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endophilin-A

Alternative name(s):
SH3 domain-containing GRB2-like protein

Gene names

Name:	endoA
Synonyms:	endo
ORF Names:	CG14296

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis. Ref.1 Ref.2
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Note: Associated with internal membranes. Expressed presynaptically at NMJs. Ref.1 Ref.2
Tissue specificity	Expression is abundant in central nervous system (brain lobes and the ventral nerve cord) starting at embryonic stage 13 through to third instar larvae. Also expressed in photoreceptors in the eye imaginal disks and the Bolwig organs of the peripheral nervous system. Ref.1 Ref.2
Disruption phenotype	Flies exhibit dramatic impairment of clathrin-mediated endocytosis. Total loss of endoA causes severe defects in motility and death at the early L2 larval stage. Ref.1
Sequence similarities	Belongs to the endophilin family. Contains 1 BAR domain. Contains 1 SH3 domain.
RNA editing	Edited at positions 129 and 137. Partially edited. Target of Adar. Ref.1 Ref.5 Ref.6

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm Membrane
Coding sequence diversity	RNA editing
Domain	Coiled coil SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neurotransmitter secretion Non-traceable author statement PubMed 10218159. Source: FlyBase regulation of synapse structure and activity Inferred from mutant phenotype PubMed 12196570PubMed 16546084. Source: FlyBase synaptic vesicle endocytosis Inferred from mutant phenotype Ref.2Ref.1PubMed 14627659PubMed 16269341. Source: FlyBase
Cellular_component	cytoplasm Inferred from direct assay Ref.2. Source: FlyBase membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lysophosphatidic acid acyltransferase activity Inferred from direct assay Ref.2. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Endophilin-A		PRO_0000285838
Regions
	Domain	18 – 248	231	BAR
	Domain	305 – 364	60	SH3
	Coiled coil	227 – 247	21	Potential
Amino acid modifications
	Modified residue	285	1	Phosphoserine Ref.7
	Modified residue	289	1	Phosphoserine Ref.7
	Modified residue	293	1	Phosphoserine Ref.7
Natural variations
	Natural variant	129	1	K → R in RNA edited version. Ref.1 Ref.6
	Natural variant	137	1	K → E in RNA edited version. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q8T390 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F6B82FAEA6FF31A9	FASTA	369	41,409
	10 20 30 40 50 60 MAFAGLKKQI NKANQYMTEK MGGAEGTKLD MDFMEMERKT DVTVELVEEL QLKTKEFLQP 70 80 90 100 110 120 NPTARAKMAA VKGISKLSGQ AKSNTYPQPE GLLAECMLTY GKKLGEDNSV FAQALVEFGE 130 140 150 160 170 180 ALKQMADVKY SLDDNIKQNF LEPLHHMQTK DLKEVMHHRK KLQGRRLDFD CKRRRQAKDD 190 200 210 220 230 240 EIRGAEDKFG ESLQLAQVGM FNLLENDTEH VSQLVTFAEA LYDFHSQCAD VLRGLQETLQ 250 260 270 280 290 300 EKRSEAESRP RNEFVPKTLL DLNLDGGGGG LNEDGTPSHI SSSASPLPSP MRSPAKSMAV 310 320 330 340 350 360 TPQRQQQPCC QALYDFEPEN PGELAFKEND IITLLNRVDD NWFEGAVNGR TGYFPQSYVQ VQVPLPNGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release." Verstreken P., Kjaerulff O., Lloyd T.E., Atkinson R., Zhou Y., Meinertzhagen I.A., Bellen H.J. Cell 109:101-112(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, RNA EDITING OF POSITION 129, DISRUPTION PHENOTYPE. Strain: Berkeley. Tissue: Head.
[2]	"Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junction." Guichet A., Wucherpfennig T., Dudu V., Etter S., Wilsch-Brauniger M., Hellwig A., Gonzalez-Gaitan M., Huttner W.B., Schmidt A.A. EMBO J. 21:1661-1672(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Berkeley. Tissue: Head.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], RNA EDITING OF POSITION 129. Strain: Berkeley. Tissue: Head.
[6]	"RNA editing in Drosophila melanogaster: new targets and functional consequences." Stapleton M., Carlson J.W., Celniker S.E. RNA 12:1922-1932(2006) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING OF POSITIONS 129 AND 137.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-285; SER-289 AND SER-293, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF426170 mRNA. Translation: AAL24818.1. AJ437141 mRNA. Translation: CAD24682.1. AE014297 Genomic DNA. Translation: AAF55606.2. BT003300 mRNA. Translation: AAO25060.1.
RefSeq	NP_001262717.1. NM_001275788.1. NP_620122.2. NM_138767.3. NP_732383.1. NM_169838.2.
UniGene	Dm.3503.
3D structure databases
HSSP	HSSP built from PDB template 1ZWW based on UniProtKB Q62420.
ProteinModelPortal	Q8T390.
SMR	Q8T390. Positions 30-251, 307-366.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8T390. 6 interactions.
MINT	MINT-880691.
Proteomic databases
PaxDb	Q8T390.
PRIDE	Q8T390.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0083698; FBpp0083112; FBgn0038659. FBtr0083699; FBpp0083113; FBgn0038659.
GeneID	42265.
KEGG	dme:Dmel_CG14296.
Organism-specific databases
CTD	42265.
FlyBase	FBgn0038659. endoA.
Phylogenomic databases
eggNOG	NOG307129.
GeneTree	ENSGT00550000074464.
InParanoid	Q8T390.
KO	K11247.
OMA	RELGDEC.
OrthoDB	EOG4QV9TJ.
PhylomeDB	Q8T390.
Gene expression databases
Bgee	Q8T390.
Family and domain databases
Gene3D	1.20.1270.60. 1 hit.
InterPro	IPR027267. AH/BAR-dom. IPR004148. BAR_dom. IPR001452. SH3_domain. IPR013315. Spectrin_alpha_SH3. [Graphical view]
Pfam	PF03114. BAR. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00452. SH3DOMAIN. PR01887. SPECTRNALPHA.
SMART	SM00721. BAR. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS51021. BAR. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	42265.
NextBio	827957.

Entry information

Entry name

SH3G3_DROME

Accession

Primary (citable) accession number: Q8T390
Secondary accession number(s): Q95V65, Q9VE25

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project