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Q8T390

- SH3G3_DROME

UniProt

Q8T390 - SH3G3_DROME

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Protein

Endophilin-A

Gene

endoA

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis.2 Publications

GO - Molecular functioni

  1. lysophosphatidic acid acyltransferase activity Source: FlyBase
  2. phospholipid binding Source: FlyBase

GO - Biological processi

  1. membrane tubulation Source: FlyBase
  2. metabolic process Source: GOC
  3. neurotransmitter secretion Source: FlyBase
  4. regulation of synapse structure and activity Source: FlyBase
  5. synaptic vesicle endocytosis Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Enzyme and pathway databases

ReactomeiREACT_236136. Recycling pathway of L1.
REACT_239629. Lysosome Vesicle Biogenesis.
REACT_244225. Golgi Associated Vesicle Biogenesis.
REACT_262116. EGFR downregulation.
REACT_262666. Retrograde neurotrophin signalling.
SignaLinkiQ8T390.

Names & Taxonomyi

Protein namesi
Recommended name:
Endophilin-A
Alternative name(s):
SH3 domain-containing GRB2-like protein
Gene namesi
Name:endoAImported
Synonyms:endo1 Publication
ORF Names:CG14296
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0038659. endoA.

Subcellular locationi

Cytoplasm 2 Publications. Membrane 2 Publications; Peripheral membrane protein 2 Publications
Note: Associated with internal membranes. Expressed presynaptically at NMJs.2 Publications

GO - Cellular componenti

  1. cytoplasm Source: FlyBase
  2. cytosol Source: FlyBase
  3. membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Flies exhibit dramatic impairment of clathrin-mediated endocytosis. Total loss of endoA causes severe defects in motility and death at the early L2 larval stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Endophilin-APRO_0000285838Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei285 – 2851Phosphoserine1 Publication
Modified residuei289 – 2891Phosphoserine1 Publication
Modified residuei293 – 2931Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8T390.
PRIDEiQ8T390.

Expressioni

Tissue specificityi

Expression is abundant in central nervous system (brain lobes and the ventral nerve cord) starting at embryonic stage 13 through to third instar larvae. Also expressed in photoreceptors in the eye imaginal disks and the Bolwig organs of the peripheral nervous system.2 Publications

Gene expression databases

BgeeiQ8T390.
ExpressionAtlasiQ8T390. differential.

Interactioni

Protein-protein interaction databases

BioGridi67269. 5 interactions.
IntActiQ8T390. 6 interactions.
MINTiMINT-880691.

Structurei

3D structure databases

ProteinModelPortaliQ8T390.
SMRiQ8T390. Positions 30-251, 307-366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 248231BARPROSITE-ProRule annotationAdd
BLAST
Domaini305 – 36460SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili227 – 24721Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the endophilin family.Sequence Analysis
Contains 1 BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG307129.
GeneTreeiENSGT00550000074464.
InParanoidiQ8T390.
KOiK11247.
OMAiENDTEHV.
OrthoDBiEOG7G1V6H.
PhylomeDBiQ8T390.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR028501. Endophilin-A.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view]
PANTHERiPTHR10661:SF120. PTHR10661:SF120. 1 hit.
PfamiPF03114. BAR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8T390-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFAGLKKQI NKANQYMTEK MGGAEGTKLD MDFMEMERKT DVTVELVEEL
60 70 80 90 100
QLKTKEFLQP NPTARAKMAA VKGISKLSGQ AKSNTYPQPE GLLAECMLTY
110 120 130 140 150
GKKLGEDNSV FAQALVEFGE ALKQMADVKY SLDDNIKQNF LEPLHHMQTK
160 170 180 190 200
DLKEVMHHRK KLQGRRLDFD CKRRRQAKDD EIRGAEDKFG ESLQLAQVGM
210 220 230 240 250
FNLLENDTEH VSQLVTFAEA LYDFHSQCAD VLRGLQETLQ EKRSEAESRP
260 270 280 290 300
RNEFVPKTLL DLNLDGGGGG LNEDGTPSHI SSSASPLPSP MRSPAKSMAV
310 320 330 340 350
TPQRQQQPCC QALYDFEPEN PGELAFKEND IITLLNRVDD NWFEGAVNGR
360
TGYFPQSYVQ VQVPLPNGN
Length:369
Mass (Da):41,409
Last modified:June 1, 2002 - v1
Checksum:iF6B82FAEA6FF31A9
GO

RNA editingi

Partially edited. Target of Adar.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti129 – 1291K → R in RNA edited version. 2 Publications
Natural varianti137 – 1371K → E in RNA edited version. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF426170 mRNA. Translation: AAL24818.1.
AJ437141 mRNA. Translation: CAD24682.1.
AE014297 Genomic DNA. Translation: AAF55606.2.
BT003300 mRNA. Translation: AAO25060.1.
RefSeqiNP_001262717.1. NM_001275788.1.
NP_620122.2. NM_138767.3.
NP_732383.1. NM_169838.2.
UniGeneiDm.3503.

Genome annotation databases

EnsemblMetazoaiFBtr0083698; FBpp0083112; FBgn0038659.
FBtr0083699; FBpp0083113; FBgn0038659.
GeneIDi42265.
KEGGidme:Dmel_CG14296.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF426170 mRNA. Translation: AAL24818.1 .
AJ437141 mRNA. Translation: CAD24682.1 .
AE014297 Genomic DNA. Translation: AAF55606.2 .
BT003300 mRNA. Translation: AAO25060.1 .
RefSeqi NP_001262717.1. NM_001275788.1.
NP_620122.2. NM_138767.3.
NP_732383.1. NM_169838.2.
UniGenei Dm.3503.

3D structure databases

ProteinModelPortali Q8T390.
SMRi Q8T390. Positions 30-251, 307-366.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 67269. 5 interactions.
IntActi Q8T390. 6 interactions.
MINTi MINT-880691.

Proteomic databases

PaxDbi Q8T390.
PRIDEi Q8T390.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0083698 ; FBpp0083112 ; FBgn0038659 .
FBtr0083699 ; FBpp0083113 ; FBgn0038659 .
GeneIDi 42265.
KEGGi dme:Dmel_CG14296.

Organism-specific databases

CTDi 42265.
FlyBasei FBgn0038659. endoA.

Phylogenomic databases

eggNOGi NOG307129.
GeneTreei ENSGT00550000074464.
InParanoidi Q8T390.
KOi K11247.
OMAi ENDTEHV.
OrthoDBi EOG7G1V6H.
PhylomeDBi Q8T390.

Enzyme and pathway databases

Reactomei REACT_236136. Recycling pathway of L1.
REACT_239629. Lysosome Vesicle Biogenesis.
REACT_244225. Golgi Associated Vesicle Biogenesis.
REACT_262116. EGFR downregulation.
REACT_262666. Retrograde neurotrophin signalling.
SignaLinki Q8T390.

Miscellaneous databases

GenomeRNAii 42265.
NextBioi 827957.

Gene expression databases

Bgeei Q8T390.
ExpressionAtlasi Q8T390. differential.

Family and domain databases

Gene3Di 1.20.1270.60. 1 hit.
InterProi IPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR028501. Endophilin-A.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view ]
PANTHERi PTHR10661:SF120. PTHR10661:SF120. 1 hit.
Pfami PF03114. BAR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTi SM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release."
    Verstreken P., Kjaerulff O., Lloyd T.E., Atkinson R., Zhou Y., Meinertzhagen I.A., Bellen H.J.
    Cell 109:101-112(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, RNA EDITING OF POSITION 129, DISRUPTION PHENOTYPE.
    Strain: Berkeley1 Publication.
    Tissue: Head1 Publication.
  2. "Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junction."
    Guichet A., Wucherpfennig T., Dudu V., Etter S., Wilsch-Brauniger M., Hellwig A., Gonzalez-Gaitan M., Huttner W.B., Schmidt A.A.
    EMBO J. 21:1661-1672(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Berkeley1 Publication.
    Tissue: HeadImported.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley1 Publication.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], RNA EDITING OF POSITION 129.
    Strain: BerkeleyImported.
    Tissue: Head.
  6. "RNA editing in Drosophila melanogaster: new targets and functional consequences."
    Stapleton M., Carlson J.W., Celniker S.E.
    RNA 12:1922-1932(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA EDITING OF POSITIONS 129 AND 137.
  7. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-285; SER-289 AND SER-293, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiSH3G3_DROME
AccessioniPrimary (citable) accession number: Q8T390
Secondary accession number(s): Q95V65, Q9VE25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3