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Q8T2T7

- TOP3_DICDI

UniProt

Q8T2T7 - TOP3_DICDI

Protein

DNA topoisomerase 3

Gene

top3

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei361 – 3611O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 3 (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase III
    Gene namesi
    Name:top3
    ORF Names:DDB_G0275257
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0275257. top3.

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 828828DNA topoisomerase 3PRO_0000330480Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0217608.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8T2T7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 149146ToprimPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi762 – 77312Gln-richAdd
    BLAST
    Compositional biasi775 – 82551Asn-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.Curated
    Contains 1 Toprim domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0550.
    KOiK03165.
    OMAiARFSNTE.
    PhylomeDBiQ8T2T7.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 4 hits.
    3.40.50.140. 1 hit.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 2 hits.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8T2T7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKRILNVAE KPSAAKEIAA ILSNKRATVR EGFSKYNKLW DFKYNILNFN    50
    DCEMTFTSVT GHLMEIDVVE QFKPWASCDP IQLFDAPIRK TVPSDKEPLK 100
    KTLEREIKKA DILILWLDCD REGENIAFEV LEVCKNAKKK FEFYRAHFSA 150
    IIPREIDRAC KNLAKPNEKD SIAVDTRMEI DLRIGAAFTR FQTLYLKKFK 200
    IISNSDNQPK TTTPANGNIL NNSNNNSSGK EIISYGPCQF PTLGFVVERY 250
    FRIVNFKPED FWHLSVVHEK MDTSSGKMIP VTFSWCRNRL FDYTAAFILY 300
    EKCLDNTEAT VVDVTSKESR YRPVPLTTIE LQKAASKKLR ISSVQTMQYA 350
    EELYTKGLIS YPRTETDSFQ AGTDLKGLIG NQASNPEWGA YASRLINNNQ 400
    FVYPKSGKNN DNSHPPIHPT SSATGLSGNL KKIYDFITRR FLACCSEESV 450
    FANTTVTIDI QGERFSETGT MVLKLGYLEV YPFDKRNDKL IPTYQKGERF 500
    TPKRIDLTKG TTVAPHYITE AELLTAMDNN KIGTDATMAT HIQTIQDRFY 550
    VKKNESNQFV PSNLGVSLVA SYELMGFEFS KPNLRAAIEA DVDKISRGQK 600
    TKQEVLLSTI EKYKQLYQLA NQNINCFDRS FREYYEPADP KGGEFRVLVS 650
    QFSRCGKCNG KMQYKSDQNP EAPKRILFCP QCIDTFDLPK NGDISQLVTS 700
    MGVPQNCPIC QYQVLSVRNP INDKSYTICP KCRNSPPDPI HKKPFHCFQC 750
    TFNCNLATGN KQQQQQQQQQ QQQTNYNRNN NNNNTNSARP ITTRTTRTTT 800
    QHRTFTASNN NFNNNNRNSD RNNNNFIF 828
    Length:828
    Mass (Da):94,639
    Last modified:June 1, 2002 - v1
    Checksum:iC0F25439F30D5618
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000013 Genomic DNA. Translation: EAL69909.2.
    RefSeqiXP_643754.2. XM_638662.2.

    Genome annotation databases

    EnsemblProtistsiDDB0234226; DDB0234226; DDB_G0275257.
    GeneIDi8619798.
    KEGGiddi:DDB_G0275257.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000013 Genomic DNA. Translation: EAL69909.2 .
    RefSeqi XP_643754.2. XM_638662.2.

    3D structure databases

    ProteinModelPortali Q8T2T7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0217608.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0234226 ; DDB0234226 ; DDB_G0275257 .
    GeneIDi 8619798.
    KEGGi ddi:DDB_G0275257.

    Organism-specific databases

    dictyBasei DDB_G0275257. top3.

    Phylogenomic databases

    eggNOGi COG0550.
    KOi K03165.
    OMAi ARFSNTE.
    PhylomeDBi Q8T2T7.

    Miscellaneous databases

    PROi Q8T2T7.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 4 hits.
    3.40.50.140. 1 hit.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 2 hits.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiTOP3_DICDI
    AccessioniPrimary (citable) accession number: Q8T2T7
    Secondary accession number(s): Q554F7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3