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Q8T2T7 (TOP3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III
Gene names
Name:top3
ORF Names:DDB_G0275257
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length828 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Keywords
   LigandDNA-binding
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchromosome

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 828828DNA topoisomerase 3
PRO_0000330480

Regions

Domain4 – 149146Toprim
Compositional bias762 – 77312Gln-rich
Compositional bias775 – 82551Asn-rich

Sites

Active site3611O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8T2T7 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: C0F25439F30D5618

FASTA82894,639
        10         20         30         40         50         60 
MTKRILNVAE KPSAAKEIAA ILSNKRATVR EGFSKYNKLW DFKYNILNFN DCEMTFTSVT 

        70         80         90        100        110        120 
GHLMEIDVVE QFKPWASCDP IQLFDAPIRK TVPSDKEPLK KTLEREIKKA DILILWLDCD 

       130        140        150        160        170        180 
REGENIAFEV LEVCKNAKKK FEFYRAHFSA IIPREIDRAC KNLAKPNEKD SIAVDTRMEI 

       190        200        210        220        230        240 
DLRIGAAFTR FQTLYLKKFK IISNSDNQPK TTTPANGNIL NNSNNNSSGK EIISYGPCQF 

       250        260        270        280        290        300 
PTLGFVVERY FRIVNFKPED FWHLSVVHEK MDTSSGKMIP VTFSWCRNRL FDYTAAFILY 

       310        320        330        340        350        360 
EKCLDNTEAT VVDVTSKESR YRPVPLTTIE LQKAASKKLR ISSVQTMQYA EELYTKGLIS 

       370        380        390        400        410        420 
YPRTETDSFQ AGTDLKGLIG NQASNPEWGA YASRLINNNQ FVYPKSGKNN DNSHPPIHPT 

       430        440        450        460        470        480 
SSATGLSGNL KKIYDFITRR FLACCSEESV FANTTVTIDI QGERFSETGT MVLKLGYLEV 

       490        500        510        520        530        540 
YPFDKRNDKL IPTYQKGERF TPKRIDLTKG TTVAPHYITE AELLTAMDNN KIGTDATMAT 

       550        560        570        580        590        600 
HIQTIQDRFY VKKNESNQFV PSNLGVSLVA SYELMGFEFS KPNLRAAIEA DVDKISRGQK 

       610        620        630        640        650        660 
TKQEVLLSTI EKYKQLYQLA NQNINCFDRS FREYYEPADP KGGEFRVLVS QFSRCGKCNG 

       670        680        690        700        710        720 
KMQYKSDQNP EAPKRILFCP QCIDTFDLPK NGDISQLVTS MGVPQNCPIC QYQVLSVRNP 

       730        740        750        760        770        780 
INDKSYTICP KCRNSPPDPI HKKPFHCFQC TFNCNLATGN KQQQQQQQQQ QQQTNYNRNN 

       790        800        810        820 
NNNNTNSARP ITTRTTRTTT QHRTFTASNN NFNNNNRNSD RNNNNFIF 

« Hide

References

[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000013 Genomic DNA. Translation: EAL69909.2.
RefSeqXP_643754.2. XM_638662.2.

3D structure databases

ProteinModelPortalQ8T2T7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0217608.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0234226; DDB0234226; DDB_G0275257.
GeneID8619798.
KEGGddi:DDB_G0275257.

Organism-specific databases

dictyBaseDDB_G0275257. top3.

Phylogenomic databases

eggNOGCOG0550.
OMAARFSNTE.
PhylomeDBQ8T2T7.

Family and domain databases

Gene3D1.10.290.10. 1 hit.
1.10.460.10. 4 hits.
3.40.50.140. 1 hit.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 2 hits.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8T2T7.

Entry information

Entry nameTOP3_DICDI
AccessionPrimary (citable) accession number: Q8T2T7
Secondary accession number(s): Q554F7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase