SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8T2T7

- TOP3_DICDI

UniProt

Q8T2T7 - TOP3_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
DNA topoisomerase 3
Gene
top3, DDB_G0275257
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei361 – 3611O-(5'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III
Gene namesi
Name:top3
ORF Names:DDB_G0275257
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0275257. top3.

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 828828DNA topoisomerase 3
PRO_0000330480Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0217608.

Structurei

3D structure databases

ProteinModelPortaliQ8T2T7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 149146Toprim
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi762 – 77312Gln-rich
Add
BLAST
Compositional biasi775 – 82551Asn-rich
Add
BLAST

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG0550.
KOiK03165.
OMAiARFSNTE.
PhylomeDBiQ8T2T7.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 4 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 2 hits.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8T2T7-1 [UniParc]FASTAAdd to Basket

« Hide

MTKRILNVAE KPSAAKEIAA ILSNKRATVR EGFSKYNKLW DFKYNILNFN    50
DCEMTFTSVT GHLMEIDVVE QFKPWASCDP IQLFDAPIRK TVPSDKEPLK 100
KTLEREIKKA DILILWLDCD REGENIAFEV LEVCKNAKKK FEFYRAHFSA 150
IIPREIDRAC KNLAKPNEKD SIAVDTRMEI DLRIGAAFTR FQTLYLKKFK 200
IISNSDNQPK TTTPANGNIL NNSNNNSSGK EIISYGPCQF PTLGFVVERY 250
FRIVNFKPED FWHLSVVHEK MDTSSGKMIP VTFSWCRNRL FDYTAAFILY 300
EKCLDNTEAT VVDVTSKESR YRPVPLTTIE LQKAASKKLR ISSVQTMQYA 350
EELYTKGLIS YPRTETDSFQ AGTDLKGLIG NQASNPEWGA YASRLINNNQ 400
FVYPKSGKNN DNSHPPIHPT SSATGLSGNL KKIYDFITRR FLACCSEESV 450
FANTTVTIDI QGERFSETGT MVLKLGYLEV YPFDKRNDKL IPTYQKGERF 500
TPKRIDLTKG TTVAPHYITE AELLTAMDNN KIGTDATMAT HIQTIQDRFY 550
VKKNESNQFV PSNLGVSLVA SYELMGFEFS KPNLRAAIEA DVDKISRGQK 600
TKQEVLLSTI EKYKQLYQLA NQNINCFDRS FREYYEPADP KGGEFRVLVS 650
QFSRCGKCNG KMQYKSDQNP EAPKRILFCP QCIDTFDLPK NGDISQLVTS 700
MGVPQNCPIC QYQVLSVRNP INDKSYTICP KCRNSPPDPI HKKPFHCFQC 750
TFNCNLATGN KQQQQQQQQQ QQQTNYNRNN NNNNTNSARP ITTRTTRTTT 800
QHRTFTASNN NFNNNNRNSD RNNNNFIF 828
Length:828
Mass (Da):94,639
Last modified:June 1, 2002 - v1
Checksum:iC0F25439F30D5618
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000013 Genomic DNA. Translation: EAL69909.2.
RefSeqiXP_643754.2. XM_638662.2.

Genome annotation databases

EnsemblProtistsiDDB0234226; DDB0234226; DDB_G0275257.
GeneIDi8619798.
KEGGiddi:DDB_G0275257.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000013 Genomic DNA. Translation: EAL69909.2 .
RefSeqi XP_643754.2. XM_638662.2.

3D structure databases

ProteinModelPortali Q8T2T7.
ModBasei Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0217608.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0234226 ; DDB0234226 ; DDB_G0275257 .
GeneIDi 8619798.
KEGGi ddi:DDB_G0275257.

Organism-specific databases

dictyBasei DDB_G0275257. top3.

Phylogenomic databases

eggNOGi COG0550.
KOi K03165.
OMAi ARFSNTE.
PhylomeDBi Q8T2T7.

Miscellaneous databases

PROi Q8T2T7.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 4 hits.
3.40.50.140. 1 hit.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 2 hits.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiTOP3_DICDI
AccessioniPrimary (citable) accession number: Q8T2T7
Secondary accession number(s): Q554F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi