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Q8T2T7

- TOP3_DICDI

UniProt

Q8T2T7 - TOP3_DICDI

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Protein

DNA topoisomerase 3

Gene

top3

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei361 – 3611O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III
Gene namesi
Name:top3
ORF Names:DDB_G0275257
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0275257. top3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 828828DNA topoisomerase 3PRO_0000330480Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0217608.

Structurei

3D structure databases

ProteinModelPortaliQ8T2T7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 149146ToprimPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi762 – 77312Gln-richAdd
BLAST
Compositional biasi775 – 82551Asn-richAdd
BLAST

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0550.
InParanoidiQ8T2T7.
KOiK03165.
OMAiARFSNTE.
PhylomeDBiQ8T2T7.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 4 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 2 hits.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8T2T7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKRILNVAE KPSAAKEIAA ILSNKRATVR EGFSKYNKLW DFKYNILNFN
60 70 80 90 100
DCEMTFTSVT GHLMEIDVVE QFKPWASCDP IQLFDAPIRK TVPSDKEPLK
110 120 130 140 150
KTLEREIKKA DILILWLDCD REGENIAFEV LEVCKNAKKK FEFYRAHFSA
160 170 180 190 200
IIPREIDRAC KNLAKPNEKD SIAVDTRMEI DLRIGAAFTR FQTLYLKKFK
210 220 230 240 250
IISNSDNQPK TTTPANGNIL NNSNNNSSGK EIISYGPCQF PTLGFVVERY
260 270 280 290 300
FRIVNFKPED FWHLSVVHEK MDTSSGKMIP VTFSWCRNRL FDYTAAFILY
310 320 330 340 350
EKCLDNTEAT VVDVTSKESR YRPVPLTTIE LQKAASKKLR ISSVQTMQYA
360 370 380 390 400
EELYTKGLIS YPRTETDSFQ AGTDLKGLIG NQASNPEWGA YASRLINNNQ
410 420 430 440 450
FVYPKSGKNN DNSHPPIHPT SSATGLSGNL KKIYDFITRR FLACCSEESV
460 470 480 490 500
FANTTVTIDI QGERFSETGT MVLKLGYLEV YPFDKRNDKL IPTYQKGERF
510 520 530 540 550
TPKRIDLTKG TTVAPHYITE AELLTAMDNN KIGTDATMAT HIQTIQDRFY
560 570 580 590 600
VKKNESNQFV PSNLGVSLVA SYELMGFEFS KPNLRAAIEA DVDKISRGQK
610 620 630 640 650
TKQEVLLSTI EKYKQLYQLA NQNINCFDRS FREYYEPADP KGGEFRVLVS
660 670 680 690 700
QFSRCGKCNG KMQYKSDQNP EAPKRILFCP QCIDTFDLPK NGDISQLVTS
710 720 730 740 750
MGVPQNCPIC QYQVLSVRNP INDKSYTICP KCRNSPPDPI HKKPFHCFQC
760 770 780 790 800
TFNCNLATGN KQQQQQQQQQ QQQTNYNRNN NNNNTNSARP ITTRTTRTTT
810 820
QHRTFTASNN NFNNNNRNSD RNNNNFIF
Length:828
Mass (Da):94,639
Last modified:June 1, 2002 - v1
Checksum:iC0F25439F30D5618
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000013 Genomic DNA. Translation: EAL69909.2.
RefSeqiXP_643754.2. XM_638662.2.

Genome annotation databases

EnsemblProtistsiDDB0234226; DDB0234226; DDB_G0275257.
GeneIDi8619798.
KEGGiddi:DDB_G0275257.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000013 Genomic DNA. Translation: EAL69909.2 .
RefSeqi XP_643754.2. XM_638662.2.

3D structure databases

ProteinModelPortali Q8T2T7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0217608.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0234226 ; DDB0234226 ; DDB_G0275257 .
GeneIDi 8619798.
KEGGi ddi:DDB_G0275257.

Organism-specific databases

dictyBasei DDB_G0275257. top3.

Phylogenomic databases

eggNOGi COG0550.
InParanoidi Q8T2T7.
KOi K03165.
OMAi ARFSNTE.
PhylomeDBi Q8T2T7.

Miscellaneous databases

PROi Q8T2T7.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 4 hits.
3.40.50.140. 1 hit.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 2 hits.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiTOP3_DICDI
AccessioniPrimary (citable) accession number: Q8T2T7
Secondary accession number(s): Q554F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3