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Q8T2K9

- MASY_DICDI

UniProt

Q8T2K9 - MASY_DICDI

Protein

Malate synthase

Gene

masA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei162 – 1621Proton acceptorBy similarity
    Active sitei449 – 4491Proton donorBy similarity

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-UniPathway
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase (EC:2.3.3.9)
    Gene namesi
    Name:masA
    ORF Names:DDB_G0275887
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0275887. masA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 543543Malate synthasePRO_0000365606Add
    BLAST

    Proteomic databases

    PRIDEiQ8T2K9.

    Expressioni

    Developmental stagei

    Late prestalk cell-specific expression.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0230181.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8T2K9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the malate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG2225.
    InParanoidiQ8T2K9.
    KOiK01638.
    OMAiSIWQWIR.
    PhylomeDBiQ8T2K9.

    Family and domain databases

    InterProiIPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001363. Malate_synth. 1 hit.
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
    PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8T2K9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRGIEIRGK VDKNVKSILT EECLTFLGEL ERRFGGIRKN LLQKRLDRQI    50
    DINNGILPSF LKDSDCKRAT DKNWKCSSVP LEIQDRRVEI TGPTDRKMVI 100
    NALNSGAKVF MADFEDANCP NWENSIHGQQ NMIDANNRTI SFTSAEGRKY 150
    ELNKQVAVLF VRPRGWHLNE DHLAIDGLSM SGSLFDFGCY IFHNHQILKN 200
    RNSNPYFYLP KMESHLEARL WNDVFVFSQN YLGMPIGTIK ATVLIETILA 250
    SFEMDEILYE LRDHSAGLNC GRWDYIFSFI KKFQSYPDKM LPDRAKVTMT 300
    SPFMDSYVKL LIYTCHKRGV HAMGGMAAQI PIKNNEQANN AAMEKVRLDK 350
    QREVRAGHDG TWVAHPALIP IAMEQFNTHM KSQNQISYIP SSTAPNFEEI 400
    AKSLLSVDPV KPGDITEDGF RSNIVVGILY LEAWLNGNGC VPIHNLMEDA 450
    ATAEISRSQI WQWIKHKAPL PDGKSFVTLQ YYNRIFKEES DKLEKAHPNS 500
    KTLTDAIQIF NNLIVSPNFI DFLTPSCYKF VVDSERKTFS PKL 543
    Length:543
    Mass (Da):62,030
    Last modified:June 1, 2003 - v2
    Checksum:i6F8E37DCCC9904B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000013 Genomic DNA. Translation: EAL69694.2.
    RefSeqiXP_643552.2. XM_638460.2.

    Genome annotation databases

    EnsemblProtistsiDDB0230181; DDB0230181; DDB_G0275887.
    GeneIDi8620134.
    KEGGiddi:DDB_G0275887.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000013 Genomic DNA. Translation: EAL69694.2 .
    RefSeqi XP_643552.2. XM_638460.2.

    3D structure databases

    ProteinModelPortali Q8T2K9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0230181.

    Proteomic databases

    PRIDEi Q8T2K9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0230181 ; DDB0230181 ; DDB_G0275887 .
    GeneIDi 8620134.
    KEGGi ddi:DDB_G0275887.

    Organism-specific databases

    dictyBasei DDB_G0275887. masA.

    Phylogenomic databases

    eggNOGi COG2225.
    InParanoidi Q8T2K9.
    KOi K01638.
    OMAi SIWQWIR.
    PhylomeDBi Q8T2K9.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .

    Miscellaneous databases

    PROi Q8T2K9.

    Family and domain databases

    InterProi IPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001363. Malate_synth. 1 hit.
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
    PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Changing patterns of gene expression in Dictyostelium prestalk cell subtypes recognized by in situ hybridization with genes from microarray analyses."
      Maeda M., Sakamoto H., Iranfar N., Fuller D., Maruo T., Ogihara S., Morio T., Urushihara H., Tanaka Y., Loomis W.F.
      Eukaryot. Cell 2:627-637(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiMASY_DICDI
    AccessioniPrimary (citable) accession number: Q8T2K9
    Secondary accession number(s): Q552X6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 3, 2009
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3