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Q8T2K9 (MASY_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase

EC=2.3.3.9
Gene names
Name:masA
ORF Names:DDB_G0275887
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length543 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Developmental stage

Late prestalk cell-specific expression. Ref.3

Sequence similarities

Belongs to the malate synthase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 543543Malate synthase
PRO_0000365606

Sites

Active site1621Proton acceptor By similarity
Active site4491Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8T2K9 [UniParc].

Last modified June 1, 2003. Version 2.
Checksum: 6F8E37DCCC9904B2

FASTA54362,030
        10         20         30         40         50         60 
MLRGIEIRGK VDKNVKSILT EECLTFLGEL ERRFGGIRKN LLQKRLDRQI DINNGILPSF 

        70         80         90        100        110        120 
LKDSDCKRAT DKNWKCSSVP LEIQDRRVEI TGPTDRKMVI NALNSGAKVF MADFEDANCP 

       130        140        150        160        170        180 
NWENSIHGQQ NMIDANNRTI SFTSAEGRKY ELNKQVAVLF VRPRGWHLNE DHLAIDGLSM 

       190        200        210        220        230        240 
SGSLFDFGCY IFHNHQILKN RNSNPYFYLP KMESHLEARL WNDVFVFSQN YLGMPIGTIK 

       250        260        270        280        290        300 
ATVLIETILA SFEMDEILYE LRDHSAGLNC GRWDYIFSFI KKFQSYPDKM LPDRAKVTMT 

       310        320        330        340        350        360 
SPFMDSYVKL LIYTCHKRGV HAMGGMAAQI PIKNNEQANN AAMEKVRLDK QREVRAGHDG 

       370        380        390        400        410        420 
TWVAHPALIP IAMEQFNTHM KSQNQISYIP SSTAPNFEEI AKSLLSVDPV KPGDITEDGF 

       430        440        450        460        470        480 
RSNIVVGILY LEAWLNGNGC VPIHNLMEDA ATAEISRSQI WQWIKHKAPL PDGKSFVTLQ 

       490        500        510        520        530        540 
YYNRIFKEES DKLEKAHPNS KTLTDAIQIF NNLIVSPNFI DFLTPSCYKF VVDSERKTFS 


PKL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Changing patterns of gene expression in Dictyostelium prestalk cell subtypes recognized by in situ hybridization with genes from microarray analyses."
Maeda M., Sakamoto H., Iranfar N., Fuller D., Maruo T., Ogihara S., Morio T., Urushihara H., Tanaka Y., Loomis W.F.
Eukaryot. Cell 2:627-637(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000013 Genomic DNA. Translation: EAL69694.2.
RefSeqXP_643552.2. XM_638460.2.

3D structure databases

ProteinModelPortalQ8T2K9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0230181.

Proteomic databases

PRIDEQ8T2K9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0230181; DDB0230181; DDB_G0275887.
GeneID8620134.
KEGGddi:DDB_G0275887.

Organism-specific databases

dictyBaseDDB_G0275887. masA.

Phylogenomic databases

eggNOGCOG2225.
InParanoidQ8T2K9.
OMASIWQWIR.
PhylomeDBQ8T2K9.

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8T2K9.

Entry information

Entry nameMASY_DICDI
AccessionPrimary (citable) accession number: Q8T2K9
Secondary accession number(s): Q552X6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase