Q8T2K9 (MASY_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase
EC=2.3.3.9

Gene names

Name:	masA
ORF Names:	DDB_G0275887

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Developmental stage	Late prestalk cell-specific expression. Ref.3
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Malate synthase		PRO_0000365606
Sites
	Active site	162	1	Proton acceptor By similarity
	Active site	449	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8T2K9 [UniParc]. Last modified June 1, 2003. Version 2. Checksum: 6F8E37DCCC9904B2	FASTA	543	62,030
	10 20 30 40 50 60 MLRGIEIRGK VDKNVKSILT EECLTFLGEL ERRFGGIRKN LLQKRLDRQI DINNGILPSF 70 80 90 100 110 120 LKDSDCKRAT DKNWKCSSVP LEIQDRRVEI TGPTDRKMVI NALNSGAKVF MADFEDANCP 130 140 150 160 170 180 NWENSIHGQQ NMIDANNRTI SFTSAEGRKY ELNKQVAVLF VRPRGWHLNE DHLAIDGLSM 190 200 210 220 230 240 SGSLFDFGCY IFHNHQILKN RNSNPYFYLP KMESHLEARL WNDVFVFSQN YLGMPIGTIK 250 260 270 280 290 300 ATVLIETILA SFEMDEILYE LRDHSAGLNC GRWDYIFSFI KKFQSYPDKM LPDRAKVTMT 310 320 330 340 350 360 SPFMDSYVKL LIYTCHKRGV HAMGGMAAQI PIKNNEQANN AAMEKVRLDK QREVRAGHDG 370 380 390 400 410 420 TWVAHPALIP IAMEQFNTHM KSQNQISYIP SSTAPNFEEI AKSLLSVDPV KPGDITEDGF 430 440 450 460 470 480 RSNIVVGILY LEAWLNGNGC VPIHNLMEDA ATAEISRSQI WQWIKHKAPL PDGKSFVTLQ 490 500 510 520 530 540 YYNRIFKEES DKLEKAHPNS KTLTDAIQIF NNLIVSPNFI DFLTPSCYKF VVDSERKTFS PKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Changing patterns of gene expression in Dictyostelium prestalk cell subtypes recognized by in situ hybridization with genes from microarray analyses." Maeda M., Sakamoto H., Iranfar N., Fuller D., Maruo T., Ogihara S., Morio T., Urushihara H., Tanaka Y., Loomis W.F. Eukaryot. Cell 2:627-637(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000013 Genomic DNA. Translation: EAL69694.2.
RefSeq	XP_643552.2. XM_638460.2.
3D structure databases
ProteinModelPortal	Q8T2K9.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0230181.
Proteomic databases
PRIDE	Q8T2K9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0230181; DDB0230181; DDB_G0275887.
GeneID	8620134.
KEGG	ddi:DDB_G0275887.
Organism-specific databases
dictyBase	DDB_G0275887. masA.
Phylogenomic databases
eggNOG	COG2225.
InParanoid	Q8T2K9.
OMA	GFDGTWV.
ProtClustDB	CLSZ2430406.
Enzyme and pathway databases
UniPathway	UPA00703; UER00720.
Family and domain databases
InterPro	IPR011076. Malate_synth-like. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_DICDI

Accession

Primary (citable) accession number: Q8T2K9
Secondary accession number(s): Q552X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)