Reviewed, UniProtKB/Swiss-Prot Q8T2J9 (MCCB_DICDI)

Names and origin

Protein names

Recommended name:
    Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
      Short name=MCCase subunit beta
    EC=6.4.1.4
Alternative name(s):
    3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta
    3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit
    3-methylcrotonyl-CoA carboxylase 2

Gene names

Name:	mccb
Synonyms:	mccc2
ORF Names:	DDB_G0271960

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA.
Pathway	Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.
Subunit structure	Probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the accD/PCCB family. Contains 1 carboxyltransferase domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methylcrotonoyl-CoA carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 588		Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial		PRO_0000327449
Regions
	Domain	78 – 582	505	Carboxyltransferase
	Region	366 – 395	30	Acyl-CoA binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8T2J9-1 [UniParc]. Last modified June 1, 2003. Version 2. Checksum: 5A5AA09C6D32FDC6	FASTA	588	64,025
	10 20 30 40 50 60 MLKSISLLKN NQILLKNIIN NGRIINNVGE KLSSKSLLKI NYSSSTTDRT FNILDGTIDK 70 80 90 100 110 120 NSAEYKDNLI NMNSTLKQLK ENIEKIKLGG GEKLNQKNIS RGKLLVRERI EALIDVGSPF 130 140 150 160 170 180 LEFSQLAGWG MYGKEEVAAG GIITGIGKIH GVECVIVAND STVKGGTYFP ITVKKHLRAQ 190 200 210 220 230 240 EIAQENNLPC IYLVDSGGAN LPRQADVFPD RDHFGRIFFN QANMSAKRIP QIAVVMGSCT 250 260 270 280 290 300 AGGAYVPAMA DESVIVKGTG TIFLGGPPLV KAATGEIVTS EELGGADLHC RTSGVTDHYA 310 320 330 340 350 360 RDDAEAIAIT RRIVSNLNRK KQPSPVITET EEPLYPTSEL AGIVPSDLKK NFDIRKVIAR 370 380 390 400 410 420 LVDGSRFDEF KELYGTTLIC GFARVHGMPV GIIANNGILF SESAVKGAHF IELCNQRGIP 430 440 450 460 470 480 LVFLQNITGF MVGKTYESKG IAKDGAKMVM AVATAKVPKI TMIIGGSFGA GNYGMCGRSY 490 500 510 520 530 540 SPRFLYMWPN AKISVMGGEQ AASVLAQIQK DNMAKENKQW SPEEENTFKK PISDKFEEEG 550 560 570 580 SIYYSSARCW DDGVIDPQDS RKVIALSLSA CMNQPINPPS DGFGVFRM

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC115599 Genomic DNA. Translation: AAL92331.2. AAFI02000007 Genomic DNA. Translation: EAL71414.1.
RefSeq	XP_645342.1.
3D structure databases
HSSP	HSSP built from PDB template 1ON3 based on UniProtKB Q8GBW6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8T2J9.
Genome annotation databases
GeneID	3396486.
GenomeReviews	Gene locus mccb in contig CM000151_GR.
KEGG	ddi:DDB_0234167.
Organism-specific databases
dictyBase	DDB_G0271960. mccb.
Phylogenomic databases
OMA	NAPIPET.
Enzyme and pathway databases
BRENDA	6.4.1.4. 424.
Family and domain databases
InterPro	IPR000022. Carboxyl_trans. IPR011763. COA_CT_C. IPR011762. COA_CT_N. [Graphical view]
Pfam	PF01039. Carboxyl_trans. 1 hit. [Graphical view]
PROSITE	PS50989. COA_CT_CTER. 1 hit. PS50980. COA_CT_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCCB_DICDI

Accession

Primary (citable) accession number: Q8T2J9
Secondary accession number(s): Q55AB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)