Q8T2I5 (LYSG3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable GH family 25 lysozyme 3
EC=3.2.1.17

Alternative name(s):
1,4-beta-N-acetylmuramidase 3

Gene names

ORF Names:

DDB_G0276439

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 25 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular space Inferred from direct assay PubMed 20422638. Source: dictyBase
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 480	460	Probable GH family 25 lysozyme 3		PRO_0000330649
Regions
	Compositional bias	219 – 473	255	Ser-rich
Sites
	Active site	116	1	By similarity
Amino acid modifications
	Glycosylation	423	1	N-linked (GlcNAc...) Potential
	Glycosylation	428	1	N-linked (GlcNAc...) Potential
	Glycosylation	437	1	N-linked (GlcNAc...) Potential
	Glycosylation	446	1	N-linked (GlcNAc...) Potential
	Glycosylation	468	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8T2I5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: DE6A8A54794891E3	FASTA	480	46,450
	10 20 30 40 50 60 MNKLILSILS VLLIVSIASA GNGIDISSGT NPSSSDLACL AKQNSKIIVQ TWSGGYGFNS 70 80 90 100 110 120 NVPSIVQAAK SAGLSVDLYA FLCSQCSGIT DPAEAIKTLV SKLNGVKYGT LWIDVEQCNG 130 140 150 160 170 180 CWSSTLSSNA QYVQTAVQTA SQLGVNVGVY SSEGEWPQTV GSLTSLSSFP LWYAHYDNNP 190 200 210 220 230 240 SFSDQGYNFG GWSKAVMKQY QGTTTQCGVS VDLNWFPGSG SSTTSSSATS SSTTGRPLTS 250 260 270 280 290 300 SSGAGTGGHS SSSGAGTGGH SSTTGGQTSG QTSGQTSGSS NQPLSSGSSQ DSSNSGSSNQ 310 320 330 340 350 360 PLSSGSSQDS SNSGSSNQPL SSGSSQTSGQ TSGSSNQPLS SGSSQDSGSN SGSQGTTGSQ 370 380 390 400 410 420 SGSSSSNPLT SGSQSGSSGS QSTSSGSQSG SSGATGTSST ASSTSATGSS STGSSTGSSS 430 440 450 460 470 480 GSNDSGSNDS GSSSGSNNSG SSSGSNNSGS SSGSASSSGS SGSSGSGNYT SGSGNGAFLF

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000015 Genomic DNA. Translation: EAL69173.1.
RefSeq	XP_643144.1. XM_638052.1.
3D structure databases
ProteinModelPortal	Q8T2I5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0252582; DDB0252582; DDB_G0276439.
GeneID	8620551.
KEGG	ddi:DDB_G0276439.
Organism-specific databases
dictyBase	DDB_G0276439.
Phylogenomic databases
eggNOG	NOG12793.
OMA	IDISSGT.
ProtClustDB	CLSZ2431205.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR002053. Glyco_hydro_25. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF01183. Glyco_hydro_25. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LYSG3_DICDI

Accession

Primary (citable) accession number: Q8T2I5
Secondary accession number(s): Q551J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)