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Protein

Cytosolic Fe-S cluster assembly factor NUBP1 homolog

Gene

nubp1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The nubp1-nubp2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of nubp1 and two labile, bridging clusters between subunits of the nubp1-nubp2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi13 – 131Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi27 – 271Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi30 – 301Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi36 – 361Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi239 – 2391Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi242 – 2421Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 738ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP1 homologUniRule annotation
Gene namesi
Name:nubp1
Synonyms:nbp1
ORF Names:DDB_G0277437
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0277437. nubp1.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Cytosolic Fe-S cluster assembly factor NUBP1 homologPRO_0000327471Add
BLAST

Proteomic databases

PaxDbiQ8T2F3.
PRIDEiQ8T2F3.

Interactioni

Subunit structurei

Heterotetramer of 2 nubp1 and 2 nubp2 chains.UniRule annotation

Protein-protein interaction databases

STRINGi44689.DDB0232424.

Structurei

3D structure databases

ProteinModelPortaliQ8T2F3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3022. Eukaryota.
COG0489. LUCA.
InParanoidiQ8T2F3.
OMAiQRIREFC.
PhylomeDBiQ8T2F3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8T2F3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQLVAPPP ENCPGTQSEM SGKSAACAGC PNQQICATAP KGPDPDIIEI
60 70 80 90 100
EERMKTVKNK ILVLSGKGGV GKSTFSSQLS FALSMDEKVE VGLLDIDICG
110 120 130 140 150
PSIPKIMGLE GENIHISGQG WDPVYVQDNL AVMSVGFLLE KEEDAVIWRG
160 170 180 190 200
PKKNGIIKQF LKDVYWNDLD YLVIDTPPGT SDEHLSIVQY LKTSNLSGAV
210 220 230 240 250
IVTSPQDVAL IDVRKEINFC KKVGVPIIGV VENMSGFVCP KCNKESQIFI
260 270 280 290 300
PTTGGAEKMS QDMNVPFLGR IPIDPLIARS CDEGKSYLIT HPNSEATKQY
310
NLIFNKIKEI VNNSK
Length:315
Mass (Da):34,256
Last modified:June 1, 2002 - v1
Checksum:i3DD726AC69AEE123
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000020 Genomic DNA. Translation: EAL68681.1.
RefSeqiXP_642627.1. XM_637535.1.

Genome annotation databases

EnsemblProtistsiDDB0232424; DDB0232424; DDB_G0277437.
GeneIDi8621044.
KEGGiddi:DDB_G0277437.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000020 Genomic DNA. Translation: EAL68681.1.
RefSeqiXP_642627.1. XM_637535.1.

3D structure databases

ProteinModelPortaliQ8T2F3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0232424.

Proteomic databases

PaxDbiQ8T2F3.
PRIDEiQ8T2F3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0232424; DDB0232424; DDB_G0277437.
GeneIDi8621044.
KEGGiddi:DDB_G0277437.

Organism-specific databases

dictyBaseiDDB_G0277437. nubp1.

Phylogenomic databases

eggNOGiKOG3022. Eukaryota.
COG0489. LUCA.
InParanoidiQ8T2F3.
OMAiQRIREFC.
PhylomeDBiQ8T2F3.

Miscellaneous databases

PROiQ8T2F3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUBP1_DICDI
AccessioniPrimary (citable) accession number: Q8T2F3
Secondary accession number(s): Q54ZL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.