Reviewed, UniProtKB/Swiss-Prot Q8T191 (UGGG_DICDI)

Names and origin

Protein names

Recommended name:
    Probable UDP-glucose:glycoprotein glucosyltransferase A
    EC=2.4.1.-
Alternative name(s):
    Developmental gene 1109 protein

Gene names

Name:	ggtA
Synonyms:	DG1109
ORF Names:	DDB_G0274103

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1681 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum By similarity.
Cofactor	Calcium or manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum lumen. Endoplasmic reticulum-Golgi intermediate compartment By similarity.
Sequence similarities	Belongs to the glycosyltransferase 8 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	ER-Golgi intermediate compartment Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-glucose:glycoprotein glucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 1681	1656	Probable UDP-glucose:glycoprotein glucosyltransferase A		PRO_0000327469
Regions
	Region	1346 – 1657	312	Glucosyltransferase By similarity
	Compositional bias	209 – 217	9	Poly-Ser
	Compositional bias	569 – 579	11	Poly-Asn
	Compositional bias	689 – 695	7	Poly-Thr
	Compositional bias	1061 – 1071	11	Poly-Ser
Amino acid modifications
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	174	1	N-linked (GlcNAc...) Potential
	Glycosylation	391	1	N-linked (GlcNAc...) Potential
	Glycosylation	1233	1	N-linked (GlcNAc...) Potential
	Glycosylation	1338	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8T191-1 [UniParc]. Last modified June 1, 2003. Version 2. Checksum: 946F082CE2E58B18	FASTA	1,681	192,629
	10 20 30 40 50 60 MARIFKFFVF LLIVFISNVL LLVESNEGDN SFSSKSIQLS LVSNWGETPS YLEAAEFLHN 70 80 90 100 110 120 QDKSLFWKFI EEFNKIDFST NYSDKIYYES TISLMKSVLS SNTQFLSEFL SIDLAMRTYS 130 140 150 160 170 180 PRVETYRQLA ISNMKLNNIE HSITTADNKT ITLFNSGGWV QIKNKIITDV NEINESLFKD 190 200 210 220 230 240 VAVVDDEENE FIRLYDFDHI FPTLANTVSS SSSSPSSIPI VILYVDIKSE FFKLVHPKLK 250 260 270 280 290 300 QFSQMGKIKY CLRYVVQESN QKLNLQGYGY ELSIKNLEYK VMDDSAIKKD IIIDGVKSKT 310 320 330 340 350 360 IINIPNEDVQ GFNFHKLQKR KPELTSKLST FRSYLMAKSQ EAKELKVWEL KDLGIQSAQK 370 380 390 400 410 420 IIQSGDPLRS LEYISQKFPT LSNSLSKITL NESLKSVIES NQKIIPSTTD QTLLLNGRLI 430 440 450 460 470 480 DTNELSPIEL SRIILEEYEH STTIQQQGPL SSKTVQDIIS AQLPIRIQLL PTKEELELNG 490 500 510 520 530 540 GNEPFVSLNN LELDYIYRQW EPKLQSSVLD KPVTSPQDIF IRKNLLTTVI VLDWNNINTF 550 560 570 580 590 600 EIIPEIQEMV QGNSLIPTRI QLLFNTKSNN NNNNNNNNND QNSQTSNFIQ GKDLAKVFLT 610 620 630 640 650 660 IKNSNLGNRG AFFFITALNY FKKMYIPNEL GITRSVLSSS FQAVLQQMGG SVRSLQHALT 670 680 690 700 710 720 NTDFDNLLES SNQLIERLEL LDTTTSQSTT TTTTTKILPK VFVNGVQVKY SNIDQLSFDL 730 740 750 760 770 780 LVSLYDEFDN LKPLFKESIL STTTAQYYET ILTSSYWKDN NLPFLKKLNS MISNEKYSHL 790 800 810 820 830 840 ITNSKNRNQE VDAQNVLKNL LYFRNNENKD EQNLLNLIVI GDFDHYNTRD ISLELLRQLE 850 860 870 880 890 900 KGELKNCKLT FISNPIDINS VVNTAGNENQ ILGKLITILK HYGKILTPQL VIGLFEKVQS 910 920 930 940 950 960 DPTIIDSFKT MKQIIELSGF DIAANDIWVA QSVNLFKQSS KVCKQYLGIQ STNKSPLSIL 970 980 990 1000 1010 1020 VNGRIITPPL SYDDAASFIQ SDFKLLLEIE MIKAKKTFEL LNSDPILKDK SNLKISDLLN 1030 1040 1050 1060 1070 1080 KVQSLVGYYY NGNNQLDSNI KRKRIPNSLS ISFSHKPPTL SSSSSSSSSN SNDVPLKFLM 1090 1100 1110 1120 1130 1140 IINPFNKVSQ KLVPMVREFS NKLNIPVDVI LNPPVSLSEL PLKTYYTYVI KLSSEFNNEN 1150 1160 1170 1180 1190 1200 VLYNQPLGIA TDIPEDRVVT LALDIPSSWL VQPIIAKYDL DNIRLKDLGD EQVLTAVYEL 1210 1220 1230 1240 1250 1260 ENIVIEGSAN DMTTDNAPAG LELLLNPIST QTNKTQDTIV MNNFGYYQLK SNPGIWKLTI 1270 1280 1290 1300 1310 1320 APGRSSDIMD MVDHPNQKEK ETFVIVPHRL VVIDSLYQSL SSLSVVRKAG QELRPILQPI 1330 1340 1350 1360 1370 1380 DEYEKQKEQE KEQKLKQNSS GFFSNLFSSK NDATDSVATH QKKSNLDTIH IFSVASGHLY 1390 1400 1410 1420 1430 1440 ERFLKIMMLS VVKNTESPIK FWFLKNYLSP AFKEFIPEMA KEYGFQYELV TYKWPWWLRK 1450 1460 1470 1480 1490 1500 QTEKQRIIWS YKILFLDVLF PLDVPKIIFV DADQVVRTDL KELWDMDLHG ASLGYTPFCD 1510 1520 1530 1540 1550 1560 SNKDTEGFRF WKSGYWRQHL AGRSYHISAL YVVDLVRFRR LAAGDQLRAT YDQLSRDPNS 1570 1580 1590 1600 1610 1620 LANLDQDLPN YLQHYVRIHS LPQEWLWCET WCDQESKSKA KTIDLCNNPL TKTPKLENAV 1630 1640 1650 1660 1670 1680 RIIDEWTTLD NEAKEFELKI DQSKHHRQIE LDHQNQLPNS KPIENIDDIL LNLAESQKDL F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The identification of Dictyostelium phosphoproteins altered in response to the activation of RasG." Secko D.M., Insall R.H., Spiegelman G.B., Weeks G. Proteomics 4:2629-2639(2004) [PubMed: 15352238] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC116986 Genomic DNA. Translation: AAM08766.2. AAFI02000012 Genomic DNA. Translation: EAL69944.1.
RefSeq	XP_644005.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT24. Glycosyltransferase Family 24.
Genome annotation databases
GeneID	3395024.
KEGG	ddi:DDB_0220030.
Organism-specific databases
dictyBase	DDB_G0274103. ggtA.
Phylogenomic databases
OMA	Q8T191. VEPVIAI.
Family and domain databases
InterPro	IPR009448. UDP-g_GGtrans. [Graphical view]
PANTHER	PTHR11226. UDP-g_GGtrans. 1 hit.
Pfam	PF06427. UDP-g_GGTase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UGGG_DICDI

Accession

Primary (citable) accession number: Q8T191
Secondary accession number(s): Q555Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)