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Q8T137

- GSHR_DICDI

UniProt

Q8T137 - GSHR_DICDI

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Protein
Glutathione reductase
Gene
gsr, DDB_G0272754
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Maintains high levels of reduced glutathione in the cytosol.1 Publication

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

Binds 1 FAD per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei454 – 4541Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 509FAD By similarity

GO - Molecular functioni

  1. NADP binding Source: dictyBase
  2. flavin adenine dinucleotide binding Source: InterPro
  3. glutathione binding Source: dictyBase
  4. glutathione-disulfide reductase activity Source: dictyBase

GO - Biological processi

  1. cell redox homeostasis Source: dictyBase
  2. culmination involved in sorocarp development Source: dictyBase
  3. glutathione metabolic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiREACT_176495. Detoxification of Reactive Oxygen Species.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Gene namesi
Name:gsr
ORF Names:DDB_G0272754
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272754. gsr.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. intracellular Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Glutathione reductase
PRO_0000327610Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 55Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ8T137.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231410.

Structurei

3D structure databases

ProteinModelPortaliQ8T137.
SMRiQ8T137. Positions 6-464.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG1249.
KOiK00383.
OMAiTERYEAT.
PhylomeDBiQ8T137.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006322. Glutathione_Rdtase_euk/bac.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF55424. SSF55424. 1 hit.
TIGRFAMsiTIGR01421. gluta_reduc_1. 1 hit.
PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8T137-1 [UniParc]FASTAAdd to Basket

« Hide

MSSTNHFTYL VLGAGSGGIA SARRAAKHLN AKGNGDRIGI VEVTRPGGTC    50
VNVGCVPKKV MWNTSFIKEM INAAPSYGFD FGGQQVKFNW PTIKKARDEY 100
IKRLNGIYDS NLAKDNIVRI NGYGRFSGPK EIQVNGANGE KYTADHILIA 150
AGGRPTVPDV PGKELGITSD GFFELEDLPK STLVVGAGYI AVELAGVLHS 200
LGSETTMVIR QKQFLRTFDE MLHTTLLKQM TDDGVKFVTE ASIKSLERDV 250
DGKRIIATTN AGVKLPPVEC VIWAIGRVPN TDDLGIDKAG IQLTEQSGFI 300
KVDEFQNTNV PGVHAVGDIC GNFLLTPVAI AAGRRLSERL FNGKSDLKFE 350
YENVATVVFS HPPIGTVGLT EQEAITKYGT ENIKCYNTSF INMFYSVQVH 400
KVRTSMKLVC LGKEEKVIGL HIIGDGCDEI IQGFAVAVKM GCTKWDLDNT 450
CAIHPTSAEE LVTMV 465
Length:465
Mass (Da):50,483
Last modified:June 1, 2003 - v2
Checksum:iE89CAF09AEA6A771
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000008 Genomic DNA. Translation: EAL71014.1.
RefSeqiXP_644939.1. XM_639847.1.

Genome annotation databases

EnsemblProtistsiDDB0231410; DDB0231410; DDB_G0272754.
GeneIDi8618618.
KEGGiddi:DDB_G0272754.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000008 Genomic DNA. Translation: EAL71014.1 .
RefSeqi XP_644939.1. XM_639847.1.

3D structure databases

ProteinModelPortali Q8T137.
SMRi Q8T137. Positions 6-464.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231410.

Proteomic databases

PRIDEi Q8T137.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231410 ; DDB0231410 ; DDB_G0272754 .
GeneIDi 8618618.
KEGGi ddi:DDB_G0272754.

Organism-specific databases

dictyBasei DDB_G0272754. gsr.

Phylogenomic databases

eggNOGi COG1249.
KOi K00383.
OMAi TERYEAT.
PhylomeDBi Q8T137.

Enzyme and pathway databases

Reactomei REACT_176495. Detoxification of Reactive Oxygen Species.

Miscellaneous databases

PROi Q8T137.

Family and domain databases

Gene3Di 3.30.390.30. 1 hit.
InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006322. Glutathione_Rdtase_euk/bac.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view ]
Pfami PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view ]
PRINTSi PR00368. FADPNR.
SUPFAMi SSF55424. SSF55424. 1 hit.
TIGRFAMsi TIGR01421. gluta_reduc_1. 1 hit.
PROSITEi PS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Reduced glutathione levels affect the culmination and cell fate decision in Dictyostelium discoideum."
    Choi C.-H., Kim B.-J., Jeong S.-Y., Lee C.-H., Kim J.-S., Park S.-J., Yim H.-S., Kang S.-O.
    Dev. Biol. 295:523-533(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGSHR_DICDI
AccessioniPrimary (citable) accession number: Q8T137
Secondary accession number(s): Q558X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

The active site is a redox-active disulfide bond.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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