Q8T137 (GSHR_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione reductase
Short name=GR
Short name=GRase
EC=1.8.1.7

Gene names

Name:	gsr
ORF Names:	DDB_G0272754

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol. Ref.3
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from mutant phenotype Ref.3. Source: dictyBase culmination involved in sorocarp development Inferred from mutant phenotype Ref.3. Source: dictyBase glutathione metabolic process Inferred from mutant phenotype Ref.3. Source: dictyBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from direct assay. Source: dictyBase
Molecular function	NADP binding Inferred from direct assay Ref.3. Source: dictyBase flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro glutathione binding Inferred from direct assay Ref.3. Source: dictyBase glutathione-disulfide reductase activity Inferred from mutant phenotype Ref.3. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Glutathione reductase		PRO_0000327610
Regions
	Nucleotide binding	42 – 50	9	FAD By similarity
Sites
	Active site	454	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	50 ↔ 55		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8T137 [UniParc]. Last modified June 1, 2003. Version 2. Checksum: E89CAF09AEA6A771	FASTA	465	50,483
	10 20 30 40 50 60 MSSTNHFTYL VLGAGSGGIA SARRAAKHLN AKGNGDRIGI VEVTRPGGTC VNVGCVPKKV 70 80 90 100 110 120 MWNTSFIKEM INAAPSYGFD FGGQQVKFNW PTIKKARDEY IKRLNGIYDS NLAKDNIVRI 130 140 150 160 170 180 NGYGRFSGPK EIQVNGANGE KYTADHILIA AGGRPTVPDV PGKELGITSD GFFELEDLPK 190 200 210 220 230 240 STLVVGAGYI AVELAGVLHS LGSETTMVIR QKQFLRTFDE MLHTTLLKQM TDDGVKFVTE 250 260 270 280 290 300 ASIKSLERDV DGKRIIATTN AGVKLPPVEC VIWAIGRVPN TDDLGIDKAG IQLTEQSGFI 310 320 330 340 350 360 KVDEFQNTNV PGVHAVGDIC GNFLLTPVAI AAGRRLSERL FNGKSDLKFE YENVATVVFS 370 380 390 400 410 420 HPPIGTVGLT EQEAITKYGT ENIKCYNTSF INMFYSVQVH KVRTSMKLVC LGKEEKVIGL 430 440 450 460 HIIGDGCDEI IQGFAVAVKM GCTKWDLDNT CAIHPTSAEE LVTMV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Reduced glutathione levels affect the culmination and cell fate decision in Dictyostelium discoideum." Choi C.-H., Kim B.-J., Jeong S.-Y., Lee C.-H., Kim J.-S., Park S.-J., Yim H.-S., Kang S.-O. Dev. Biol. 295:523-533(2006) [PubMed: 16678813] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000008 Genomic DNA. Translation: EAL71014.1.
RefSeq	XP_644939.1. XM_639847.1.
3D structure databases
HSSP	HSSP built from PDB template 1ALG based on UniProtKB P00390.
ProteinModelPortal	Q8T137.
SMR	Q8T137. Positions 6-464.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8T137.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231410; DDB0231410; DDB_G0272754.
GeneID	8618618.
GenomeReviews	Gene locus gsr in contig CM000151_GR.
KEGG	ddi:DDB_G0272754.
Organism-specific databases
dictyBase	DDB_G0272754. gsr.
Phylogenomic databases
eggNOG	KOG0405.
GeneTree	EPrGT00050000003671.
HOGENOM	HBG515043.
OMA	KVVGLHM.
PhylomeDB	Q8T137.
Family and domain databases
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006322. Glutathione_Rdtase_euk/bac. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD-bd_dom. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KO	K00383.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
TIGRFAMs	TIGR01421. Gluta_reduc_1. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHR_DICDI

Accession

Primary (citable) accession number: Q8T137
Secondary accession number(s): Q558X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)