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Protein

Glutathione reductase

Gene

gsr

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Maintains high levels of reduced glutathione in the cytosol.1 Publication

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei454Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 50FADBy similarity9

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: InterPro
  • glutathione binding Source: dictyBase
  • glutathione-disulfide reductase activity Source: dictyBase
  • NADP binding Source: dictyBase

GO - Biological processi

  • cell redox homeostasis Source: dictyBase
  • culmination involved in sorocarp development Source: dictyBase
  • glutathione metabolic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiR-DDI-3299685. Detoxification of Reactive Oxygen Species.
R-DDI-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-DDI-5628897. TP53 Regulates Metabolic Genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Gene namesi
Name:gsr
ORF Names:DDB_G0272754
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272754. gsr.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • intracellular Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003276101 – 465Glutathione reductaseAdd BLAST465

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 55Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8T137.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231410.

Structurei

3D structure databases

ProteinModelPortaliQ8T137.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0405. Eukaryota.
COG1249. LUCA.
InParanoidiQ8T137.
KOiK00383.
OMAiARMKLVC.
PhylomeDBiQ8T137.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR006322. Glutathione_Rdtase_euk/bac.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR012999. Pyr_OxRdtase_I_AS.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
TIGRFAMsiTIGR01421. gluta_reduc_1. 1 hit.
PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8T137-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTNHFTYL VLGAGSGGIA SARRAAKHLN AKGNGDRIGI VEVTRPGGTC
60 70 80 90 100
VNVGCVPKKV MWNTSFIKEM INAAPSYGFD FGGQQVKFNW PTIKKARDEY
110 120 130 140 150
IKRLNGIYDS NLAKDNIVRI NGYGRFSGPK EIQVNGANGE KYTADHILIA
160 170 180 190 200
AGGRPTVPDV PGKELGITSD GFFELEDLPK STLVVGAGYI AVELAGVLHS
210 220 230 240 250
LGSETTMVIR QKQFLRTFDE MLHTTLLKQM TDDGVKFVTE ASIKSLERDV
260 270 280 290 300
DGKRIIATTN AGVKLPPVEC VIWAIGRVPN TDDLGIDKAG IQLTEQSGFI
310 320 330 340 350
KVDEFQNTNV PGVHAVGDIC GNFLLTPVAI AAGRRLSERL FNGKSDLKFE
360 370 380 390 400
YENVATVVFS HPPIGTVGLT EQEAITKYGT ENIKCYNTSF INMFYSVQVH
410 420 430 440 450
KVRTSMKLVC LGKEEKVIGL HIIGDGCDEI IQGFAVAVKM GCTKWDLDNT
460
CAIHPTSAEE LVTMV
Length:465
Mass (Da):50,483
Last modified:June 1, 2003 - v2
Checksum:iE89CAF09AEA6A771
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000008 Genomic DNA. Translation: EAL71014.1.
RefSeqiXP_644939.1. XM_639847.1.

Genome annotation databases

EnsemblProtistsiEAL71014; EAL71014; DDB_G0272754.
GeneIDi8618618.
KEGGiddi:DDB_G0272754.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000008 Genomic DNA. Translation: EAL71014.1.
RefSeqiXP_644939.1. XM_639847.1.

3D structure databases

ProteinModelPortaliQ8T137.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231410.

Proteomic databases

PaxDbiQ8T137.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL71014; EAL71014; DDB_G0272754.
GeneIDi8618618.
KEGGiddi:DDB_G0272754.

Organism-specific databases

dictyBaseiDDB_G0272754. gsr.

Phylogenomic databases

eggNOGiKOG0405. Eukaryota.
COG1249. LUCA.
InParanoidiQ8T137.
KOiK00383.
OMAiARMKLVC.
PhylomeDBiQ8T137.

Enzyme and pathway databases

ReactomeiR-DDI-3299685. Detoxification of Reactive Oxygen Species.
R-DDI-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-DDI-5628897. TP53 Regulates Metabolic Genes.

Miscellaneous databases

PROiQ8T137.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR006322. Glutathione_Rdtase_euk/bac.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR012999. Pyr_OxRdtase_I_AS.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
TIGRFAMsiTIGR01421. gluta_reduc_1. 1 hit.
PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSHR_DICDI
AccessioniPrimary (citable) accession number: Q8T137
Secondary accession number(s): Q558X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

The active site is a redox-active disulfide bond.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.