Unreviewed, UniProtKB/TrEMBL Q8T0L3 (Q8T0L3_DROME)

Names and origin

Protein names

Recommended name:
    Ubiquitin-activating enzyme E1 RuleBase RU004072V0

Gene names

Name:	Uba1 EMBL AAL39336.1 FlyBase FBgn0023143
ORF Names:	CG1782 FlyBase FBgn0023143, Dmel_CG1782 EMBL AAF58910.2

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome] EMBL AAL39336.1

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activates ubiquitin by first adenylating with ATP its carboxy-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP By similarity. RuleBase RU004072V0
Pathway	Protein modification; protein ubiquitination. RuleBase RU004072V0
Subunit structure	Monomer By similarity. RuleBase RU004072V0
Sequence similarities	Belongs to the ubiquitin-activating E1 family. RuleBase RU000519V1

Ontologies

Keywords
Biological process	Ubl conjugation pathway RuleBase RU000519V1
Ligand	ATP-binding RuleBase RU000519V1 Nucleotide-binding
Molecular function	Ligase RuleBase RU000519V1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	neuron remodeling Inferred from mutant phenotype. Source: FlyBase protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	dendrite Inferred from mutant phenotype. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ligase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct small protein activating enzyme activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8T0L3-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 6B9551EEE9738902	FASTA	1,191	130,758
	10 20 30 40 50 60 MSSGQQLMAD SPACSSSDHS PLAKKRRLEL LGDGVGAMGM EEEEAGGATS SSSSNNYENH 70 80 90 100 110 120 DKAGGVDISG KCQSKTTSEH QLELCVSSST SDTCGVNAAA KRAEKQKLKQ SQGSGDDVST 130 140 150 160 170 180 SSAVEKEGSK RPEKRNYLNT TTIESQEASS SASATSNSNN NVSSSNNNSN NSTNNRQLEG 190 200 210 220 230 240 SSNMAGNSAA AGGDIDESLY SRQLYVLGHD AMRRMANSDI LLSGLGGLGL EIAKNVILGG 250 260 270 280 290 300 VKSITLHDTA TCGLHDLSSQ FYLTEADIGK NRAEASCAQL AELNNYVRTV SHTGPLTEEF 310 320 330 340 350 360 LRKFRVVVLT NSDGEEQQRI AKFAHENGIA LIIAETRGLF AKVFCDFGES FTIYDQDGTQ 370 380 390 400 410 420 PISTMIASIT HDAQGVVTCL DETRHGFNDG DYVTFSEVQG MQELNGCQPL KITVLGPYTF 430 440 450 460 470 480 SIGDTSKFGE YKSGGVATQV KMPKTISFKP LAQATEEPEF LISDFAKLDS PATLHVAFNA 490 500 510 520 530 540 LSCYRKAHNG ALPRPWNEED ANSFLEVVRA SSNAEVDEKL VLQFAKICSG NTCPLDAAVG 550 560 570 580 590 600 GIVAQEVLKA CSGKFTPIYQ WLYFDALECL PTEGVEEADA QPVGSRYDSQ IAIFGKKFQE 610 620 630 640 650 660 KLADSKWFIV GAGAIGCELL KNFGMLGLGT GNGQIFVTDM DLIEKSNLNR QFLFRPHDVQ 670 680 690 700 710 720 KPKSMTAADA IKRMNPEVNV TAYELRVGAE TEKVFSEDFF GKLDGVANAL DNVDARIYMD 730 740 750 760 770 780 RKCIFNRIPL VETGTLGTLG NVQVIVPFAT ESYSSSQDPP EKSIPICTLK NFPNAIEHTL 790 800 810 820 830 840 QWARDAFEGV FKQSAENAAQ YIADPQFTER IAKLPGIQPL EILDSIKKAL IDDKPKSFAH 850 860 870 880 890 900 CVEWARLYWE DQYVNQIKQL LFNFPPDQIT SSGQPFWSGP KRCPDPLVFD VNDPMHLDFI 910 920 930 940 950 960 YAAANLRAEV YGIEQVRNRE TIAELVQKVK VPEFKPRSGV KIETNEAAAA ASANNFDDGE 970 980 990 1000 1010 1020 LDQDRVDKII SELLKNADKS SKITPLEFEK DDDSNLHMDF IVACSNLRAA NYKIPPADRH 1030 1040 1050 1060 1070 1080 KSKLIAGKII PAIATTTSVL SGLAVLEVIK LIVGHRDLVK FKNGFANLAL PFMAFSEPLP 1090 1100 1110 1120 1130 1140 AAKNTYYGKE WTLWDRFEVT GELSLQEFLN YFEENEKLKI TMLSQGVSML YSFFMPKAKC 1150 1160 1170 1180 1190 SERLPLPMSE VVRRVSKRRL EPHERSLVFE ICCNDVDGED VEVPYVRYTL P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M., Chavez C., Dorsett V., Farfan D., Frise E., George R., Gonzalez M., Guarin H., Li P., Liao G., Miranda A., Mungall C.J., Nunoo J. , Pacleb J., Paragas V., Park S., Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Berkeley EMBL AAL39336.1.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013599 Genomic DNA. Translation: AAF58910.2. AY069191 mRNA. Translation: AAL39336.1.
RefSeq	NP_477310.2.
UniGene	Dm.5488
3D structure databases
HSSP	HSSP built from PDB template 1JW9 based on UniProtKB P12282.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8T0L3. 4 interactions.
Proteomic databases
PRIDE	Q8T0L3.
Genome annotation databases
Ensembl	FBgn0023143. Drosophila melanogaster. [Contig view]
GeneID	35998.
KEGG	dme:Dmel_CG1782.
NMPDR	fig|7227.3.peg.4125.
Organism-specific databases
FlyBase	FBgn0023143. Uba1.
Phylogenomic databases
HOGENOM	Q8T0L3.
OMA	Q8T0L3. LERVSEC.
Gene expression databases
ArrayExpress	Q8T0L3.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR000594. ThiF_NAD_FAD_bd. IPR018965. Ub-activating_enz_e1_C. IPR000127. UBact_repeat. IPR019572. Ubiquitin-activating_enzyme. IPR018075. UBQ-activ_enz_E1. IPR000011. UBQ-activ_enz_E1-like. IPR018074. UBQ-activ_enz_E1_AS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
Pfam	PF00899. ThiF. 2 hits. PF09358. UBA_e1_C. 1 hit. PF10585. UBA_e1_thiolCys. 1 hit. PF02134. UBACT. 2 hits. [Graphical view]
PRINTS	PR01849. UBIQUITINACT.
TIGRFAMs	TIGR01408. Ube1. 1 hit.
PROSITE	PS00536. UBIQUITIN_ACTIVAT_1. 1 hit. PS00865. UBIQUITIN_ACTIVAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	796260.

Entry information

Entry name

Q8T0L3_DROME

Accession

Primary (citable) accession number: Q8T0L3
Secondary accession number(s): Q9V5A7

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2002
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)