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Q8T045

- RUMI_DROME

UniProt

Q8T045 - RUMI_DROME

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Protein

O-glucosyltransferase rumi

Gene

rumi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Protein O-glucosyltransferase. Catalyzes the reaction that attaches glucose through an O-glycosidic linkage to a conserved serine residue in epidermal growth factor-like repeats. Regulates Notch signaling by glucosylating Notch in the ER, glucosylation is required for the correct folding and cleavage of Notch.1 Publication

Pathwayi

GO - Molecular functioni

  1. glucosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. Golgi organization Source: FlyBase
  2. negative regulation of Notch signaling pathway Source: UniProtKB
  3. Notch signaling pathway Source: FlyBase
  4. positive regulation of Notch signaling pathway Source: FlyBase
  5. protein glycosylation in endoplasmic reticulum Source: UniProtKB
  6. regulation of stem cell division Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Notch signaling pathway

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT90. Glycosyltransferase Family 90.

Names & Taxonomyi

Protein namesi
Recommended name:
O-glucosyltransferase rumi (EC:2.4.1.-)
Gene namesi
Name:rumiImported
ORF Names:CG31152
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0086253. rumi.

Subcellular locationi

Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Flies exhibit a temperature-dependent loss of Notch signaling, resulting in bristle loss.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi189 – 1891G → E in rumi-79; complete loss of enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 411391O-glucosyltransferase rumiSequence AnalysisPRO_0000342687Add
BLAST

Proteomic databases

PaxDbiQ8T045.
PRIDEiQ8T045.

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiQ8T045.

Interactioni

Protein-protein interaction databases

BioGridi77376. 1 interaction.
MINTiMINT-1699273.
STRINGi7227.FBpp0083713.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi408 – 4114Prevents secretion from ER1 PublicationPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase 90 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG248922.
GeneTreeiENSGT00530000063132.
InParanoidiQ8T045.
KOiK13667.
OMAiINHLQMD.
OrthoDBiEOG7R831J.
PhylomeDBiQ8T045.

Family and domain databases

InterProiIPR006598. LipoPS_modifying.
[Graphical view]
PfamiPF05686. Glyco_transf_90. 1 hit.
[Graphical view]
SMARTiSM00672. CAP10. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8T045-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLINHLIVVL LISLVGTGGA EDDGLCSADQ KSCAQSEPDQ INEDEFSFKI
60 70 80 90 100
RRQIEKANAD YKPCSSDPQD SDCSCHANVL KRDLAPYKST GVTRQMIESS
110 120 130 140 150
ARYGTKYKIY GHRLYRDANC MFPARCEGIE HFLLPLVATL PDMDLIINTR
160 170 180 190 200
DYPQLNAAWG NAAGGPVFSF SKTKEYRDIM YPAWTFWAGG PATKLHPRGI
210 220 230 240 250
GRWDQMREKL EKRAAAIPWS QKRSLGFFRG SRTSDERDSL ILLSRRNPEL
260 270 280 290 300
VEAQYTKNQG WKSPKDTLDA PAADEVSFED HCKYKYLFNF RGVAASFRLK
310 320 330 340 350
HLFLCKSLVF HVGDEWQEFF YDQLKPWVHY VPLKSYPSQQ EYEHILSFFK
360 370 380 390 400
KNDALAQEIA QRGYDFIWEH LRMKDIKCYW RKLLKRYVKL LQYEVKPEDQ
410
LIYIGPKKDE L
Length:411
Mass (Da):47,735
Last modified:June 1, 2002 - v1
Checksum:i785EA3A15977B69D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014297 Genomic DNA. Translation: AAN13920.1.
AY069564 mRNA. Translation: AAL39709.1.
RefSeqiNP_651095.1. NM_142838.4.
UniGeneiDm.1286.

Genome annotation databases

EnsemblMetazoaiFBtr0084320; FBpp0083713; FBgn0086253.
GeneIDi326122.
KEGGidme:Dmel_CG31152.
UCSCiCG31152-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014297 Genomic DNA. Translation: AAN13920.1 .
AY069564 mRNA. Translation: AAL39709.1 .
RefSeqi NP_651095.1. NM_142838.4.
UniGenei Dm.1286.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 77376. 1 interaction.
MINTi MINT-1699273.
STRINGi 7227.FBpp0083713.

Protein family/group databases

CAZyi GT90. Glycosyltransferase Family 90.

Proteomic databases

PaxDbi Q8T045.
PRIDEi Q8T045.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0084320 ; FBpp0083713 ; FBgn0086253 .
GeneIDi 326122.
KEGGi dme:Dmel_CG31152.
UCSCi CG31152-RA. d. melanogaster.

Organism-specific databases

CTDi 326122.
FlyBasei FBgn0086253. rumi.

Phylogenomic databases

eggNOGi NOG248922.
GeneTreei ENSGT00530000063132.
InParanoidi Q8T045.
KOi K13667.
OMAi INHLQMD.
OrthoDBi EOG7R831J.
PhylomeDBi Q8T045.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

GenomeRNAii 326122.
NextBioi 847167.
PROi Q8T045.

Gene expression databases

Bgeei Q8T045.

Family and domain databases

InterProi IPR006598. LipoPS_modifying.
[Graphical view ]
Pfami PF05686. Glyco_transf_90. 1 hit.
[Graphical view ]
SMARTi SM00672. CAP10. 1 hit.
[Graphical view ]
PROSITEi PS00014. ER_TARGET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley1 Publication.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BerkeleyImported.
    Tissue: Embryo1 Publication.
  4. "Rumi is a CAP10 domain glycosyltransferase that modifies Notch and is required for Notch signaling."
    Acar M., Jafar-Nejad H., Takeuchi H., Rajan A., Ibrani D., Rana N.A., Pan H., Haltiwanger R.S., Bellen H.J.
    Cell 132:247-258(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLY-189, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiRUMI_DROME
AccessioniPrimary (citable) accession number: Q8T045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3