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Q8T045

- RUMI_DROME

UniProt

Q8T045 - RUMI_DROME

Protein

O-glucosyltransferase rumi

Gene

rumi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Protein O-glucosyltransferase. Catalyzes the reaction that attaches glucose through an O-glycosidic linkage to a conserved serine residue in epidermal growth factor-like repeats. Regulates Notch signaling by glucosylating Notch in the ER, glucosylation is required for the correct folding and cleavage of Notch.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. glucosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. Golgi organization Source: FlyBase
    2. negative regulation of Notch signaling pathway Source: UniProtKB
    3. Notch signaling pathway Source: FlyBase
    4. positive regulation of Notch signaling pathway Source: FlyBase
    5. protein glycosylation in endoplasmic reticulum Source: UniProtKB
    6. regulation of stem cell division Source: FlyBase

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Notch signaling pathway

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT90. Glycosyltransferase Family 90.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    O-glucosyltransferase rumi (EC:2.4.1.-)
    Gene namesi
    Name:rumiImported
    ORF Names:CG31152
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0086253. rumi.

    Subcellular locationi

    Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Disruption phenotypei

    Flies exhibit a temperature-dependent loss of Notch signaling, resulting in bristle loss.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi189 – 1891G → E in rumi-79; complete loss of enzyme activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 411391O-glucosyltransferase rumiSequence AnalysisPRO_0000342687Add
    BLAST

    Proteomic databases

    PaxDbiQ8T045.
    PRIDEiQ8T045.

    Expressioni

    Developmental stagei

    Expressed both maternally and zygotically.1 Publication

    Gene expression databases

    BgeeiQ8T045.

    Interactioni

    Protein-protein interaction databases

    BioGridi77376. 1 interaction.
    MINTiMINT-1699273.
    STRINGi7227.FBpp0083713.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi408 – 4114Prevents secretion from ER1 PublicationPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the glycosyltransferase 90 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG248922.
    GeneTreeiENSGT00530000063132.
    InParanoidiQ8T045.
    KOiK13667.
    OMAiINHLQMD.
    OrthoDBiEOG7R831J.
    PhylomeDBiQ8T045.

    Family and domain databases

    InterProiIPR006598. LipoPS_modifying.
    [Graphical view]
    PfamiPF05686. Glyco_transf_90. 1 hit.
    [Graphical view]
    SMARTiSM00672. CAP10. 1 hit.
    [Graphical view]
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8T045-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLINHLIVVL LISLVGTGGA EDDGLCSADQ KSCAQSEPDQ INEDEFSFKI    50
    RRQIEKANAD YKPCSSDPQD SDCSCHANVL KRDLAPYKST GVTRQMIESS 100
    ARYGTKYKIY GHRLYRDANC MFPARCEGIE HFLLPLVATL PDMDLIINTR 150
    DYPQLNAAWG NAAGGPVFSF SKTKEYRDIM YPAWTFWAGG PATKLHPRGI 200
    GRWDQMREKL EKRAAAIPWS QKRSLGFFRG SRTSDERDSL ILLSRRNPEL 250
    VEAQYTKNQG WKSPKDTLDA PAADEVSFED HCKYKYLFNF RGVAASFRLK 300
    HLFLCKSLVF HVGDEWQEFF YDQLKPWVHY VPLKSYPSQQ EYEHILSFFK 350
    KNDALAQEIA QRGYDFIWEH LRMKDIKCYW RKLLKRYVKL LQYEVKPEDQ 400
    LIYIGPKKDE L 411
    Length:411
    Mass (Da):47,735
    Last modified:June 1, 2002 - v1
    Checksum:i785EA3A15977B69D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAN13920.1.
    AY069564 mRNA. Translation: AAL39709.1.
    RefSeqiNP_651095.1. NM_142838.3.
    UniGeneiDm.1286.

    Genome annotation databases

    EnsemblMetazoaiFBtr0084320; FBpp0083713; FBgn0086253.
    GeneIDi326122.
    KEGGidme:Dmel_CG31152.
    UCSCiCG31152-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAN13920.1 .
    AY069564 mRNA. Translation: AAL39709.1 .
    RefSeqi NP_651095.1. NM_142838.3.
    UniGenei Dm.1286.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 77376. 1 interaction.
    MINTi MINT-1699273.
    STRINGi 7227.FBpp0083713.

    Protein family/group databases

    CAZyi GT90. Glycosyltransferase Family 90.

    Proteomic databases

    PaxDbi Q8T045.
    PRIDEi Q8T045.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0084320 ; FBpp0083713 ; FBgn0086253 .
    GeneIDi 326122.
    KEGGi dme:Dmel_CG31152.
    UCSCi CG31152-RA. d. melanogaster.

    Organism-specific databases

    CTDi 326122.
    FlyBasei FBgn0086253. rumi.

    Phylogenomic databases

    eggNOGi NOG248922.
    GeneTreei ENSGT00530000063132.
    InParanoidi Q8T045.
    KOi K13667.
    OMAi INHLQMD.
    OrthoDBi EOG7R831J.
    PhylomeDBi Q8T045.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    GenomeRNAii 326122.
    NextBioi 847167.
    PROi Q8T045.

    Gene expression databases

    Bgeei Q8T045.

    Family and domain databases

    InterProi IPR006598. LipoPS_modifying.
    [Graphical view ]
    Pfami PF05686. Glyco_transf_90. 1 hit.
    [Graphical view ]
    SMARTi SM00672. CAP10. 1 hit.
    [Graphical view ]
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley1 Publication.
    2. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: BerkeleyImported.
      Tissue: Embryo1 Publication.
    4. "Rumi is a CAP10 domain glycosyltransferase that modifies Notch and is required for Notch signaling."
      Acar M., Jafar-Nejad H., Takeuchi H., Rajan A., Ibrani D., Rana N.A., Pan H., Haltiwanger R.S., Bellen H.J.
      Cell 132:247-258(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLY-189, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiRUMI_DROME
    AccessioniPrimary (citable) accession number: Q8T045
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3