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Protein

O-glucosyltransferase rumi

Gene

rumi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein O-glucosyltransferase. Catalyzes the reaction that attaches glucose through an O-glycosidic linkage to a conserved serine residue in epidermal growth factor-like repeats. Regulates Notch signaling by glucosylating Notch in the ER, glucosylation is required for the correct folding and cleavage of Notch.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • glycolipid metabolic process Source: GO_Central
  • Golgi organization Source: FlyBase
  • negative regulation of Notch signaling pathway Source: UniProtKB
  • Notch signaling pathway Source: FlyBase
  • positive regulation of Notch signaling pathway Source: FlyBase
  • protein glycosylation in endoplasmic reticulum Source: UniProtKB
  • protein O-linked glycosylation Source: GO_Central
  • regulation of stem cell division Source: FlyBase
  • rhabdomere development Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Notch signaling pathway

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT90. Glycosyltransferase Family 90.

Names & Taxonomyi

Protein namesi
Recommended name:
O-glucosyltransferase rumi (EC:2.4.1.-)
Gene namesi
Name:rumiImported
ORF Names:CG31152
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0086253. rumi.

Subcellular locationi

GO - Cellular componenti

  • endomembrane system Source: FlyBase
  • endoplasmic reticulum lumen Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Flies exhibit a temperature-dependent loss of Notch signaling, resulting in bristle loss.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189G → E in rumi-79; complete loss of enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000034268721 – 411O-glucosyltransferase rumiSequence analysisAdd BLAST391

Proteomic databases

PaxDbiQ8T045.
PRIDEiQ8T045.

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0086253.
ExpressionAtlasiQ8T045. baseline.
GenevisibleiQ8T045. DM.

Interactioni

Protein-protein interaction databases

BioGridi77376. 1 interactor.
MINTiMINT-1699273.
STRINGi7227.FBpp0083713.

Structurei

Secondary structure

1411
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi43 – 60Combined sources18
Beta strandi64 – 67Combined sources4
Helixi77 – 84Combined sources8
Helixi85 – 87Combined sources3
Turni88 – 90Combined sources3
Helixi94 – 100Combined sources7
Helixi101 – 103Combined sources3
Beta strandi104 – 110Combined sources7
Beta strandi113 – 116Combined sources4
Helixi123 – 133Combined sources11
Helixi134 – 139Combined sources6
Beta strandi143 – 147Combined sources5
Beta strandi150 – 152Combined sources3
Beta strandi156 – 159Combined sources4
Beta strandi168 – 170Combined sources3
Helixi184 – 186Combined sources3
Beta strandi194 – 196Combined sources3
Helixi203 – 216Combined sources14
Helixi219 – 221Combined sources3
Beta strandi222 – 231Combined sources10
Helixi235 – 237Combined sources3
Helixi238 – 246Combined sources9
Turni248 – 250Combined sources3
Beta strandi251 – 256Combined sources6
Helixi264 – 267Combined sources4
Helixi278 – 281Combined sources4
Beta strandi284 – 290Combined sources7
Beta strandi293 – 295Combined sources3
Helixi298 – 303Combined sources6
Turni304 – 306Combined sources3
Beta strandi308 – 312Combined sources5
Helixi321 – 323Combined sources3
Turni326 – 328Combined sources3
Beta strandi329 – 333Combined sources5
Beta strandi335 – 337Combined sources3
Helixi339 – 350Combined sources12
Helixi353 – 370Combined sources18
Helixi373 – 388Combined sources16
Beta strandi401 – 405Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F84X-ray2.50A21-407[»]
5F85X-ray2.15A21-407[»]
5F86X-ray1.90A21-407[»]
5F87X-ray3.20A/B/C/D/E/F21-407[»]
ProteinModelPortaliQ8T045.
SMRiQ8T045.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi408 – 411Prevents secretion from ERPROSITE-ProRule annotation1 Publication4

Sequence similaritiesi

Belongs to the glycosyltransferase 90 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2458. Eukaryota.
ENOG410XT5U. LUCA.
GeneTreeiENSGT00530000063132.
InParanoidiQ8T045.
KOiK13667.
OMAiKSYPSQQ.
OrthoDBiEOG091G0BPU.
PhylomeDBiQ8T045.

Family and domain databases

InterProiIPR006598. LipoPS_modifying.
[Graphical view]
PfamiPF05686. Glyco_transf_90. 1 hit.
[Graphical view]
SMARTiSM00672. CAP10. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8T045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLINHLIVVL LISLVGTGGA EDDGLCSADQ KSCAQSEPDQ INEDEFSFKI
60 70 80 90 100
RRQIEKANAD YKPCSSDPQD SDCSCHANVL KRDLAPYKST GVTRQMIESS
110 120 130 140 150
ARYGTKYKIY GHRLYRDANC MFPARCEGIE HFLLPLVATL PDMDLIINTR
160 170 180 190 200
DYPQLNAAWG NAAGGPVFSF SKTKEYRDIM YPAWTFWAGG PATKLHPRGI
210 220 230 240 250
GRWDQMREKL EKRAAAIPWS QKRSLGFFRG SRTSDERDSL ILLSRRNPEL
260 270 280 290 300
VEAQYTKNQG WKSPKDTLDA PAADEVSFED HCKYKYLFNF RGVAASFRLK
310 320 330 340 350
HLFLCKSLVF HVGDEWQEFF YDQLKPWVHY VPLKSYPSQQ EYEHILSFFK
360 370 380 390 400
KNDALAQEIA QRGYDFIWEH LRMKDIKCYW RKLLKRYVKL LQYEVKPEDQ
410
LIYIGPKKDE L
Length:411
Mass (Da):47,735
Last modified:June 1, 2002 - v1
Checksum:i785EA3A15977B69D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAN13920.1.
AY069564 mRNA. Translation: AAL39709.1.
RefSeqiNP_651095.1. NM_142838.4.
UniGeneiDm.1286.

Genome annotation databases

EnsemblMetazoaiFBtr0084320; FBpp0083713; FBgn0086253.
GeneIDi326122.
KEGGidme:Dmel_CG31152.
UCSCiCG31152-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAN13920.1.
AY069564 mRNA. Translation: AAL39709.1.
RefSeqiNP_651095.1. NM_142838.4.
UniGeneiDm.1286.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F84X-ray2.50A21-407[»]
5F85X-ray2.15A21-407[»]
5F86X-ray1.90A21-407[»]
5F87X-ray3.20A/B/C/D/E/F21-407[»]
ProteinModelPortaliQ8T045.
SMRiQ8T045.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi77376. 1 interactor.
MINTiMINT-1699273.
STRINGi7227.FBpp0083713.

Protein family/group databases

CAZyiGT90. Glycosyltransferase Family 90.

Proteomic databases

PaxDbiQ8T045.
PRIDEiQ8T045.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0084320; FBpp0083713; FBgn0086253.
GeneIDi326122.
KEGGidme:Dmel_CG31152.
UCSCiCG31152-RA. d. melanogaster.

Organism-specific databases

CTDi326122.
FlyBaseiFBgn0086253. rumi.

Phylogenomic databases

eggNOGiKOG2458. Eukaryota.
ENOG410XT5U. LUCA.
GeneTreeiENSGT00530000063132.
InParanoidiQ8T045.
KOiK13667.
OMAiKSYPSQQ.
OrthoDBiEOG091G0BPU.
PhylomeDBiQ8T045.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

GenomeRNAii326122.
PROiQ8T045.

Gene expression databases

BgeeiFBgn0086253.
ExpressionAtlasiQ8T045. baseline.
GenevisibleiQ8T045. DM.

Family and domain databases

InterProiIPR006598. LipoPS_modifying.
[Graphical view]
PfamiPF05686. Glyco_transf_90. 1 hit.
[Graphical view]
SMARTiSM00672. CAP10. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRUMI_DROME
AccessioniPrimary (citable) accession number: Q8T045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.