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Protein

O-glucosyltransferase rumi

Gene

rumi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein O-glucosyltransferase. Catalyzes the reaction that attaches glucose through an O-glycosidic linkage to a conserved serine residue in epidermal growth factor-like repeats. Regulates Notch signaling by glucosylating Notch in the ER, glucosylation is required for the correct folding and cleavage of Notch.1 Publication

Pathwayi

GO - Molecular functioni

  1. glucosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. Golgi organization Source: FlyBase
  2. negative regulation of Notch signaling pathway Source: UniProtKB
  3. Notch signaling pathway Source: FlyBase
  4. positive regulation of Notch signaling pathway Source: FlyBase
  5. protein glycosylation in endoplasmic reticulum Source: UniProtKB
  6. regulation of stem cell division Source: FlyBase
  7. rhabdomere development Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Notch signaling pathway

Enzyme and pathway databases

ReactomeiREACT_320947. Pre-NOTCH Processing in the Endoplasmic Reticulum.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT90. Glycosyltransferase Family 90.

Names & Taxonomyi

Protein namesi
Recommended name:
O-glucosyltransferase rumi (EC:2.4.1.-)
Gene namesi
Name:rumiImported
ORF Names:CG31152
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0086253. rumi.

Subcellular locationi

  1. Endoplasmic reticulum lumen PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  1. endomembrane system Source: FlyBase
  2. endoplasmic reticulum lumen Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Flies exhibit a temperature-dependent loss of Notch signaling, resulting in bristle loss.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi189 – 1891G → E in rumi-79; complete loss of enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 411391O-glucosyltransferase rumiSequence AnalysisPRO_0000342687Add
BLAST

Proteomic databases

PaxDbiQ8T045.
PRIDEiQ8T045.

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiQ8T045.

Interactioni

Protein-protein interaction databases

BioGridi77376. 1 interaction.
MINTiMINT-1699273.
STRINGi7227.FBpp0083713.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi408 – 4114Prevents secretion from ERPROSITE-ProRule annotation1 Publication

Sequence similaritiesi

Belongs to the glycosyltransferase 90 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG248922.
GeneTreeiENSGT00530000063132.
InParanoidiQ8T045.
KOiK13667.
OMAiPARCEGI.
OrthoDBiEOG7R831J.
PhylomeDBiQ8T045.

Family and domain databases

InterProiIPR006598. LipoPS_modifying.
[Graphical view]
PfamiPF05686. Glyco_transf_90. 1 hit.
[Graphical view]
SMARTiSM00672. CAP10. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8T045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLINHLIVVL LISLVGTGGA EDDGLCSADQ KSCAQSEPDQ INEDEFSFKI
60 70 80 90 100
RRQIEKANAD YKPCSSDPQD SDCSCHANVL KRDLAPYKST GVTRQMIESS
110 120 130 140 150
ARYGTKYKIY GHRLYRDANC MFPARCEGIE HFLLPLVATL PDMDLIINTR
160 170 180 190 200
DYPQLNAAWG NAAGGPVFSF SKTKEYRDIM YPAWTFWAGG PATKLHPRGI
210 220 230 240 250
GRWDQMREKL EKRAAAIPWS QKRSLGFFRG SRTSDERDSL ILLSRRNPEL
260 270 280 290 300
VEAQYTKNQG WKSPKDTLDA PAADEVSFED HCKYKYLFNF RGVAASFRLK
310 320 330 340 350
HLFLCKSLVF HVGDEWQEFF YDQLKPWVHY VPLKSYPSQQ EYEHILSFFK
360 370 380 390 400
KNDALAQEIA QRGYDFIWEH LRMKDIKCYW RKLLKRYVKL LQYEVKPEDQ
410
LIYIGPKKDE L
Length:411
Mass (Da):47,735
Last modified:June 1, 2002 - v1
Checksum:i785EA3A15977B69D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAN13920.1.
AY069564 mRNA. Translation: AAL39709.1.
RefSeqiNP_651095.1. NM_142838.4.
UniGeneiDm.1286.

Genome annotation databases

EnsemblMetazoaiFBtr0084320; FBpp0083713; FBgn0086253.
GeneIDi326122.
KEGGidme:Dmel_CG31152.
UCSCiCG31152-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAN13920.1.
AY069564 mRNA. Translation: AAL39709.1.
RefSeqiNP_651095.1. NM_142838.4.
UniGeneiDm.1286.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi77376. 1 interaction.
MINTiMINT-1699273.
STRINGi7227.FBpp0083713.

Protein family/group databases

CAZyiGT90. Glycosyltransferase Family 90.

Proteomic databases

PaxDbiQ8T045.
PRIDEiQ8T045.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0084320; FBpp0083713; FBgn0086253.
GeneIDi326122.
KEGGidme:Dmel_CG31152.
UCSCiCG31152-RA. d. melanogaster.

Organism-specific databases

CTDi326122.
FlyBaseiFBgn0086253. rumi.

Phylogenomic databases

eggNOGiNOG248922.
GeneTreeiENSGT00530000063132.
InParanoidiQ8T045.
KOiK13667.
OMAiPARCEGI.
OrthoDBiEOG7R831J.
PhylomeDBiQ8T045.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_320947. Pre-NOTCH Processing in the Endoplasmic Reticulum.

Miscellaneous databases

GenomeRNAii326122.
NextBioi847167.
PROiQ8T045.

Gene expression databases

BgeeiQ8T045.

Family and domain databases

InterProiIPR006598. LipoPS_modifying.
[Graphical view]
PfamiPF05686. Glyco_transf_90. 1 hit.
[Graphical view]
SMARTiSM00672. CAP10. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley1 Publication.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BerkeleyImported.
    Tissue: Embryo1 Publication.
  4. "Rumi is a CAP10 domain glycosyltransferase that modifies Notch and is required for Notch signaling."
    Acar M., Jafar-Nejad H., Takeuchi H., Rajan A., Ibrani D., Rana N.A., Pan H., Haltiwanger R.S., Bellen H.J.
    Cell 132:247-258(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLY-189, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiRUMI_DROME
AccessioniPrimary (citable) accession number: Q8T045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: June 1, 2002
Last modified: April 29, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.