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Protein

Protein Gawky

Gene

gw

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for gene silencing mediated by micro-RNAs (miRNAs). Silences both polyadenylated and deadenylated mRNAs. Required for miRNA-mediated translational repression and mRNA decay. Not required for miRNA target recognition. Necessary to initiate but not to maintain silencing. Promotes mRNA deadenylation through the recruitment of the CCR4-NOT and PAN complexes and promotes decapping by the DCP1-DCP2 complex. Dissociates from silenced mRNAs after deadenylation. Required for completion of nuclear divisions during early embryonic development.6 Publications

GO - Molecular functioni

GO - Biological processi

  • embryonic development via the syncytial blastoderm Source: FlyBase
  • gene silencing by miRNA Source: UniProtKB
  • locomotor rhythm Source: FlyBase
  • miRNA mediated inhibition of translation Source: InterPro
  • mRNA catabolic process Source: FlyBase
  • regulation of protein localization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

RNA-mediated gene silencing, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-DME-426496. Post-transcriptional silencing by small RNAs.
R-DME-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GawkyImported
Gene namesi
Name:gw
Synonyms:GW1821 Publication
ORF Names:CG31992
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 4

Organism-specific databases

FlyBaseiFBgn0051992. gw.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic mRNA processing body Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004159481 – 1384Protein GawkyAdd BLAST1384

Proteomic databases

PaxDbiQ8SY33.
PRIDEiQ8SY33.

Expressioni

Developmental stagei

Highest levels are found during early embryonic development until approximately 18 hours and during pupariation.1 Publication

Gene expression databases

BgeeiFBgn0051992.
GenevisibleiQ8SY33. DM.

Interactioni

Subunit structurei

Interacts (via N-terminal region) with AGO1 (via Piwi domain); the interaction is essential for localization of AGO1 in P-bodies and for miRNA-mediated silencing. Interacts with pAbp/PABPC1; this interaction interferes with the binding of pAbp to eIF4G and is required for miRNA-mediated silencing. Interacts with CCR4-NOT complex members Not1, Rga/NOT2, twin/CCR4, Pop2 and NOT3/5 and with PAN complex members CG8232/PAN2 and CG11486/PAN3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AGO1Q32KD43EBI-160693,EBI-105513
CG11486-RCD3DMN92EBI-160693,EBI-6512959
Not1A8DY812EBI-160693,EBI-3428401
pAbpP2118711EBI-160693,EBI-103658
PAN2A1Z7K92EBI-160693,EBI-193297

Protein-protein interaction databases

BioGridi68639. 12 interactors.
DIPiDIP-35525N.
IntActiQ8SY33. 12 interactors.
MINTiMINT-764572.
STRINGi7227.FBpp0088165.

Structurei

Secondary structure

11384
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1118 – 1122Combined sources5
Helixi1131 – 1140Combined sources10
Beta strandi1143 – 1149Combined sources7
Turni1150 – 1153Combined sources4
Beta strandi1154 – 1161Combined sources8
Helixi1162 – 1172Combined sources11
Beta strandi1175 – 1177Combined sources3
Beta strandi1180 – 1185Combined sources6
Helixi1189 – 1196Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WBRNMR-A1114-1198[»]
ProteinModelPortaliQ8SY33.
SMRiQ8SY33.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8SY33.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini547 – 588UBAPROSITE-ProRule annotationAdd BLAST42
Domaini1117 – 1189RRMSequence analysisAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 605Sufficient for miRNA-mediated silencing1 PublicationAdd BLAST605
Regioni1 – 205Required for interaction with AGO11 PublicationAdd BLAST205
Regioni205 – 490Minimal N-terminal region required for miRNA-mediated silencing1 PublicationAdd BLAST286
Regioni605 – 830Sufficient for miRNA-mediated silencing1 PublicationAdd BLAST226
Regioni862 – 1115Not required for interaction with AGO1 or miRNAs or for localization to P-bodies but necessary for miRNA-mediated silencing and for interaction with pAbp3 PublicationsAdd BLAST254
Regioni940 – 1215Sufficient for miRNA-mediated silencing1 PublicationAdd BLAST276
Regioni1200 – 1384Not required for interaction with AGO1 or miRNAs or for localization to P-bodies but necessary for miRNA-mediated silencing, dissociation from AGO1 and miRNAs and interaction with pAbp3 PublicationsAdd BLAST185

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi81 – 281Gly-richSequence analysisAdd BLAST201
Compositional biasi527 – 530Poly-GlnSequence analysis4
Compositional biasi713 – 824Gln-richSequence analysisAdd BLAST112
Compositional biasi1204 – 1366Ser-richSequence analysisAdd BLAST163

Domaini

The UBA domain is not required for correct subcellular location, gene silencing or interaction with pAbp.1 Publication
The RRM domain lacks RNA-binding properties and does not bind RNA in vitro. It is not required for P-body localization or for interaction with AGO1 or miRNAs but is required for silencing. May play a role in protein-protein interactions.1 Publication1 Publication

Sequence similaritiesi

Belongs to the GW182 family.Sequence analysis
Contains 1 RRM (RNA recognition motif) domain.Sequence analysis
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFAU. Eukaryota.
ENOG41110AX. LUCA.
GeneTreeiENSGT00410000025966.
InParanoidiQ8SY33.
KOiK18412.
OMAiDLGSEWN.
OrthoDBiEOG091G0JQM.
PhylomeDBiQ8SY33.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR019486. Argonaute_hook_dom.
IPR033503. GW182.
IPR026805. GW182_M_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR13020:SF25. PTHR13020:SF25. 5 hits.
PfamiPF10427. Ago_hook. 1 hit.
PF12938. M_domain. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q8SY33-1) [UniParc]FASTAAdd to basket
Also known as: B, E, F

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MREALFSQDG WGCQHVNQDT NWEVPSSPEP ANKDAPGPPM WKPSINNGTD
60 70 80 90 100
LWESNLRNGG QPAAQQVPKP SWGHTPSSNL GGTWGEDDDG ADSSSVWTGG
110 120 130 140 150
AVSNAGSGAA VGVNQAGVNV GPGGVVSSGG PQWGQGVVGV GLGSTGGNGS
160 170 180 190 200
SNITGSSGVA TGSSGNSSNA GNGWGDPREI RPLGVGGSMD IRNVEHRGGN
210 220 230 240 250
GSGATSSDPR DIRMIDPRDP IRGDPRGISG RLNGTSEMWG HHPQMSHNQL
260 270 280 290 300
QGINKMVGQS VATASTSVGT SGSGIGPGGP GPSTVSGNIP TQWGPAQPVS
310 320 330 340 350
VGVSGPKDMS KQISGWEEPS PPPQRRSIPN YDDGTSLWGQ QTRVPAASGH
360 370 380 390 400
WKDMTDSIGR SSHLMRGQSQ TGGIGIAGVG NSNVPVGANP SNPISSVVGP
410 420 430 440 450
QARIPSVGGV QHKPDGGAMW VHSGNVGGRN NVAAVTTWGD DTHSVNVGAP
460 470 480 490 500
SSGSVSSNNW VDDKSNSTLA QNSWSDPAPV GVSWGNKQSK PPSNSASSGW
510 520 530 540 550
STAAGVVDGV DLGSEWNTHG GIIGKSQQQQ KLAGLNVGMV NVINAEIIKQ
560 570 580 590 600
SKQYRILVEN GFKKEDVERA LVIANMNIEE AADMLRANSS LSMDGWRRHD
610 620 630 640 650
ESLGSYADHN SSTSSGGFAG RYPVNSGQPS MSFPHNNLMN NMGGTAVTGG
660 670 680 690 700
NNNTNMTALQ VQKYLNQGQH GVAVGPQAVG NSSAVSVGFG QNTSNAAVAG
710 720 730 740 750
AASVNIAANT NNQPSGQQIR MLGQQIQLAI HSGFISSQIL TQPLTQTTLN
760 770 780 790 800
LLNQLLSNIK HLQAAQQSLT RGGNVNPMAV NVAISKYKQQ IQNLQNQINA
810 820 830 840 850
QQAVYVKQQN MQPTSQQQQP QQQQLPSVHL SNSGNDYLRG HDAINNLQSN
860 870 880 890 900
FSELNINKPS GYQGASNQQS RLNQWKLPVL DKEINSDSTE FSRAPGATKQ
910 920 930 940 950
NLTANTSNIN SLGLQNDSTW STGRSIGDGW PDPSSDNENK DWSVAQPTSA
960 970 980 990 1000
ATAYTDLVQE FEPGKPWKGS QIKSIEDDPS ITPGSVARSP LSINSTPKDA
1010 1020 1030 1040 1050
DIFANTGKNS PTDLPPLSLS SSTWSFNPNQ NYPSHSWSDN SQQCTATSEL
1060 1070 1080 1090 1100
WTSPLNKSSS RGPPPGLTAN SNKSANSNAS TPTTITGGAN GWLQPRSGGV
1110 1120 1130 1140 1150
QTTNTNWTGG NTTWGSSWLL LKNLTAQIDG PTLRTLCMQH GPLVSFHPYL
1160 1170 1180 1190 1200
NQGIALCKYT TREEANKAQM ALNNCVLANT TIFAESPSEN EVQSIMQHLP
1210 1220 1230 1240 1250
QTPSSTSSSG TSGGNVGGVG TSANNANSGS AACLSGNNSG NGNGSASGAG
1260 1270 1280 1290 1300
SGNNGNSSCN NSAAGGGSSS NNTITTVANS NLVGSSGSVS NSSGVTANSS
1310 1320 1330 1340 1350
TVSVVSCTAS GNSINGAGTA NSSGSKSSAN NLASGQSSAS NLTNSTNSTW
1360 1370 1380
RQTSQNQALQ SQSRPSGREA DFDYISLVYS IVDD
Length:1,384
Mass (Da):142,973
Last modified:June 1, 2002 - v1
Checksum:i4E87DFE49050883E
GO
Isoform I (identifier: Q8SY33-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     969-971: Missing.

Note: No experimental confirmation available.
Show »
Length:1,381
Mass (Da):142,701
Checksum:i9823EFFFE67253C4
GO
Isoform J (identifier: Q8SY33-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1035-1036: Missing.

Note: No experimental confirmation available.
Show »
Length:1,382
Mass (Da):142,749
Checksum:iBCBC248F4836B070
GO

Sequence cautioni

The sequence AAM50720 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047895969 – 971Missing in isoform I. Curated3
Alternative sequenceiVSP_0478961035 – 1036Missing in isoform J. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014135 Genomic DNA. Translation: AAF59322.2.
AE014135 Genomic DNA. Translation: AAF59323.2.
AE014135 Genomic DNA. Translation: AAN06507.2.
AE014135 Genomic DNA. Translation: AAN06508.1.
AE014135 Genomic DNA. Translation: AAN06509.1.
AE014135 Genomic DNA. Translation: AAX52511.2.
AY075429 mRNA. Translation: AAL68245.1.
AY118860 mRNA. Translation: AAM50720.1. Different initiation.
RefSeqiNP_001014691.2. NM_001014691.2. [Q8SY33-3]
NP_726596.1. NM_166780.2. [Q8SY33-1]
NP_726597.1. NM_166781.2. [Q8SY33-1]
NP_726599.2. NM_166783.2. [Q8SY33-2]
NP_726600.1. NM_166784.2. [Q8SY33-1]
NP_726601.1. NM_166785.2. [Q8SY33-1]
UniGeneiDm.1403.

Genome annotation databases

EnsemblMetazoaiFBtr0089096; FBpp0088165; FBgn0051992. [Q8SY33-1]
FBtr0089097; FBpp0088166; FBgn0051992. [Q8SY33-1]
FBtr0089100; FBpp0088169; FBgn0051992. [Q8SY33-1]
FBtr0089101; FBpp0088170; FBgn0051992. [Q8SY33-1]
GeneIDi43808.
KEGGidme:Dmel_CG31992.
UCSCiCG31992-RA. d. melanogaster. [Q8SY33-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014135 Genomic DNA. Translation: AAF59322.2.
AE014135 Genomic DNA. Translation: AAF59323.2.
AE014135 Genomic DNA. Translation: AAN06507.2.
AE014135 Genomic DNA. Translation: AAN06508.1.
AE014135 Genomic DNA. Translation: AAN06509.1.
AE014135 Genomic DNA. Translation: AAX52511.2.
AY075429 mRNA. Translation: AAL68245.1.
AY118860 mRNA. Translation: AAM50720.1. Different initiation.
RefSeqiNP_001014691.2. NM_001014691.2. [Q8SY33-3]
NP_726596.1. NM_166780.2. [Q8SY33-1]
NP_726597.1. NM_166781.2. [Q8SY33-1]
NP_726599.2. NM_166783.2. [Q8SY33-2]
NP_726600.1. NM_166784.2. [Q8SY33-1]
NP_726601.1. NM_166785.2. [Q8SY33-1]
UniGeneiDm.1403.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WBRNMR-A1114-1198[»]
ProteinModelPortaliQ8SY33.
SMRiQ8SY33.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68639. 12 interactors.
DIPiDIP-35525N.
IntActiQ8SY33. 12 interactors.
MINTiMINT-764572.
STRINGi7227.FBpp0088165.

Proteomic databases

PaxDbiQ8SY33.
PRIDEiQ8SY33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089096; FBpp0088165; FBgn0051992. [Q8SY33-1]
FBtr0089097; FBpp0088166; FBgn0051992. [Q8SY33-1]
FBtr0089100; FBpp0088169; FBgn0051992. [Q8SY33-1]
FBtr0089101; FBpp0088170; FBgn0051992. [Q8SY33-1]
GeneIDi43808.
KEGGidme:Dmel_CG31992.
UCSCiCG31992-RA. d. melanogaster. [Q8SY33-1]

Organism-specific databases

CTDi43808.
FlyBaseiFBgn0051992. gw.

Phylogenomic databases

eggNOGiENOG410IFAU. Eukaryota.
ENOG41110AX. LUCA.
GeneTreeiENSGT00410000025966.
InParanoidiQ8SY33.
KOiK18412.
OMAiDLGSEWN.
OrthoDBiEOG091G0JQM.
PhylomeDBiQ8SY33.

Enzyme and pathway databases

ReactomeiR-DME-426496. Post-transcriptional silencing by small RNAs.
R-DME-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

ChiTaRSigw. fly.
EvolutionaryTraceiQ8SY33.
GenomeRNAii43808.
PROiQ8SY33.

Gene expression databases

BgeeiFBgn0051992.
GenevisibleiQ8SY33. DM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR019486. Argonaute_hook_dom.
IPR033503. GW182.
IPR026805. GW182_M_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR13020:SF25. PTHR13020:SF25. 5 hits.
PfamiPF10427. Ago_hook. 1 hit.
PF12938. M_domain. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAWKY_DROME
AccessioniPrimary (citable) accession number: Q8SY33
Secondary accession number(s): Q8MSF0, Q9V4F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.