Q8SWR8 (ATX2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ataxin-2 homolog
Short name=Datx2

Gene names

Name:	Atx2
ORF Names:	CG5166

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1084 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of actin filament formation, though it does not directly assemble with actin filaments. Required for oocyte specification and oocyte positioning in the female germline. Also required for normal eye development and bristle morphology. Ref.4
Subcellular location	Cytoplasm Ref.4.
Developmental stage	Expressed both maternally and zygotically. Zygotic expression is seen in third larval instar, pupae, and adults. Ref.4
Disruption phenotype	Flies lacking Atx2 cause female sterility, aberrant sensory bristle morphology, loss or degeneration of tissues, and lethality. Ref.4
Sequence similarities	Belongs to the ataxin-2 family.
RNA editing	Edited at positions 381 and 398. Partially edited. Target of Adar. Ref.3 Ref.5
Sequence caution	The sequence AAL39639.1 differs from that shown. Reason: Erroneous initiation. The sequence AAN71028.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAN71028.1 differs from that shown. Reason: Potential poly-A sequence. The sequence AAN71095.1 differs from that shown. Reason:

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing RNA editing
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chaeta development Inferred from mutant phenotype Ref.4. Source: UniProtKB compound eye photoreceptor development Inferred from mutant phenotype PubMed 18271626. Source: FlyBase oocyte differentiation Inferred from mutant phenotype Ref.4. Source: UniProtKB phagocytosis, engulfment Inferred from mutant phenotype PubMed 16336044. Source: FlyBase positive regulation of neuron death Inferred from mutant phenotype PubMed 18271626. Source: FlyBase regulation of actin filament polymerization Inferred from mutant phenotype Ref.4. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform B Ref.1 (identifier: Q8SWR8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A Ref.1 (identifier: Q8SWR8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     936-949: PPMAASQMHVSASA → RKSILPIIKHFIFF
     950-1084: Missing.

Note: No experimental confirmation available.

Isoform C Ref.1 (identifier: Q8SWR8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1084	1084	Ataxin-2 homolog		PRO_0000311703
Regions
	Compositional bias	272 – 303	32	Arg-rich
	Compositional bias	497 – 782	286	Gln-rich
	Compositional bias	799 – 895	97	Thr-rich
	Compositional bias	963 – 1084	122	Gln-rich
Amino acid modifications
	Modified residue	208	1	Phosphoserine Ref.7
	Modified residue	211	1	Phosphoserine Ref.7
	Modified residue	221	1	Phosphoserine Ref.7
	Modified residue	266	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 61	61	Missing in isoform A and isoform C. Ref.1		VSP_052610
	Alternative sequence	936 – 949	14	PPMAA…VSASA → RKSILPIIKHFIFF in isoform A. Ref.1		VSP_052611
	Alternative sequence	950 – 1084	135	Missing in isoform A. Ref.1		VSP_052612
	Natural variant	381	1	K → R in RNA edited version. Ref.5
	Natural variant	398	1	K → R in RNA edited version. Ref.5
Experimental info
	Sequence conflict	368	1	T → A in AAL13482. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 626F4A60A652E8E2	FASTA	1,084	117,578
	10 20 30 40 50 60 MNNNSKRKTR PTGGGASGGI SRYNSNDNSL RPTNNKAGAG GGNGGAAVRP SAQGVYNNTF 70 80 90 100 110 120 FMHSATALVG SVVEVRLRSG NIYEGVFRTF SGNFDIALEL PACIKSKNLP EEGKVPKHII 130 140 150 160 170 180 FPADTVVTIV AKDFDSQYAT AGAFQTDGAI SDKCNGARPD EKELEPWDSG ANGDIDIELD 190 200 210 220 230 240 SAANGWDPNE MFRKNENTFG VTSTFDDSLA SYTVPLDKGD SLEFKEAEAK AEKLAAEIEN 250 260 270 280 290 300 NPTCRDRLDL ENGDEEALFA AVERPSTEQD QRGDRGDRER NDRDREREER DRDRDRDRGN 310 320 330 340 350 360 KPRGAGDFQL RETMSSDRYI TKQTRSITGP QLSHVGMSSQ GSGRDRDTRG DGSMMMQSGG 370 380 390 400 410 420 GSGQGGSTQS TAALMLAGGL KGVGPAPSAN ASADSSSKYS GGSMVKRKTV PQGGKVMRNN 430 440 450 460 470 480 VPTGGSNVSV SQGGNGNSVG QNKGGYQPSM GMPSQYSYQG NSQIMHGSSQ YRNQSHMGGA 490 500 510 520 530 540 NKLNGDSNAN TNKPLPQRQM RQYQGSQSNS SLNYGGEPQS LGKPVHGSHG GHPGQNSNSP 550 560 570 580 590 600 PLQTAGPQQQ QQQQQQQQQQ QQQQQPPQQQ QHQNIQPQGQ NTQPARQVRT RDNQMQELRQ 610 620 630 640 650 660 FGQDFQLAPS NTSPPQQQQQ QQQQQQQHQV QQQQQRALQQ SASPPQQQQQ QQQQQQHVVL 670 680 690 700 710 720 HQVPQTHLHQ AALSQPHYVP QQQPQQAPLP QQQHVPHHMQ QKAQQQQLVE TQHQHVQKQH 730 740 750 760 770 780 QSQPQVQQPP PQLLQDPSQQ PLPIYHTMPP PQTSPVVVTS PVLLEQPPPQ PMPVVQQQQT 790 800 810 820 830 840 QQLATPKPEV SPAPPSSNTT TPTGIASTPT AGVIASAGSE KTTPAAPTPT SNSATVPTGT 850 860 870 880 890 900 AATAGGATGT TPVVKKHVLN PSAKPFTPRG PSTPNPSRPH TPQTPVPMTN IYTTTGGHVP 910 920 930 940 950 960 PAANQPIYVM QPQHPFPPQT HPQAGQPPRL RRSNYPPMAA SQMHVSASAA TGQPLITAGP 970 980 990 1000 1010 1020 IPQFIQYGHA PHQQQFQSHT YAPMQMRVYP DQPQQLQFMT QTPQSTTPSP GQPHQQFHPP 1030 1040 1050 1060 1070 1080 PQPSPAGGGP QPAFTPPTQA ATYQLMCVHP QSLLANHYFP PPTPQHPQQN QQQYQIVMQQ HQPQ
	Isoform A [UniParc]. Checksum: 4779D0B42CCADC76	FASTA	888	96,675
	10 20 30 40 50 60 MHSATALVGS VVEVRLRSGN IYEGVFRTFS GNFDIALELP ACIKSKNLPE EGKVPKHIIF 70 80 90 100 110 120 PADTVVTIVA KDFDSQYATA GAFQTDGAIS DKCNGARPDE KELEPWDSGA NGDIDIELDS 130 140 150 160 170 180 AANGWDPNEM FRKNENTFGV TSTFDDSLAS YTVPLDKGDS LEFKEAEAKA EKLAAEIENN 190 200 210 220 230 240 PTCRDRLDLE NGDEEALFAA VERPSTEQDQ RGDRGDRERN DRDREREERD RDRDRDRGNK 250 260 270 280 290 300 PRGAGDFQLR ETMSSDRYIT KQTRSITGPQ LSHVGMSSQG SGRDRDTRGD GSMMMQSGGG 310 320 330 340 350 360 SGQGGSTQST AALMLAGGLK GVGPAPSANA SADSSSKYSG GSMVKRKTVP QGGKVMRNNV 370 380 390 400 410 420 PTGGSNVSVS QGGNGNSVGQ NKGGYQPSMG MPSQYSYQGN SQIMHGSSQY RNQSHMGGAN 430 440 450 460 470 480 KLNGDSNANT NKPLPQRQMR QYQGSQSNSS LNYGGEPQSL GKPVHGSHGG HPGQNSNSPP 490 500 510 520 530 540 LQTAGPQQQQ QQQQQQQQQQ QQQQPPQQQQ HQNIQPQGQN TQPARQVRTR DNQMQELRQF 550 560 570 580 590 600 GQDFQLAPSN TSPPQQQQQQ QQQQQQHQVQ QQQQRALQQS ASPPQQQQQQ QQQQQHVVLH 610 620 630 640 650 660 QVPQTHLHQA ALSQPHYVPQ QQPQQAPLPQ QQHVPHHMQQ KAQQQQLVET QHQHVQKQHQ 670 680 690 700 710 720 SQPQVQQPPP QLLQDPSQQP LPIYHTMPPP QTSPVVVTSP VLLEQPPPQP MPVVQQQQTQ 730 740 750 760 770 780 QLATPKPEVS PAPPSSNTTT PTGIASTPTA GVIASAGSEK TTPAAPTPTS NSATVPTGTA 790 800 810 820 830 840 ATAGGATGTT PVVKKHVLNP SAKPFTPRGP STPNPSRPHT PQTPVPMTNI YTTTGGHVPP 850 860 870 880 AANQPIYVMQ PQHPFPPQTH PQAGQPPRLR RSNYRKSILP IIKHFIFF
	Isoform C [UniParc]. Checksum: 8ECC22F77908BCA2	FASTA	1,023	111,409
	10 20 30 40 50 60 MHSATALVGS VVEVRLRSGN IYEGVFRTFS GNFDIALELP ACIKSKNLPE EGKVPKHIIF 70 80 90 100 110 120 PADTVVTIVA KDFDSQYATA GAFQTDGAIS DKCNGARPDE KELEPWDSGA NGDIDIELDS 130 140 150 160 170 180 AANGWDPNEM FRKNENTFGV TSTFDDSLAS YTVPLDKGDS LEFKEAEAKA EKLAAEIENN 190 200 210 220 230 240 PTCRDRLDLE NGDEEALFAA VERPSTEQDQ RGDRGDRERN DRDREREERD RDRDRDRGNK 250 260 270 280 290 300 PRGAGDFQLR ETMSSDRYIT KQTRSITGPQ LSHVGMSSQG SGRDRDTRGD GSMMMQSGGG 310 320 330 340 350 360 SGQGGSTQST AALMLAGGLK GVGPAPSANA SADSSSKYSG GSMVKRKTVP QGGKVMRNNV 370 380 390 400 410 420 PTGGSNVSVS QGGNGNSVGQ NKGGYQPSMG MPSQYSYQGN SQIMHGSSQY RNQSHMGGAN 430 440 450 460 470 480 KLNGDSNANT NKPLPQRQMR QYQGSQSNSS LNYGGEPQSL GKPVHGSHGG HPGQNSNSPP 490 500 510 520 530 540 LQTAGPQQQQ QQQQQQQQQQ QQQQPPQQQQ HQNIQPQGQN TQPARQVRTR DNQMQELRQF 550 560 570 580 590 600 GQDFQLAPSN TSPPQQQQQQ QQQQQQHQVQ QQQQRALQQS ASPPQQQQQQ QQQQQHVVLH 610 620 630 640 650 660 QVPQTHLHQA ALSQPHYVPQ QQPQQAPLPQ QQHVPHHMQQ KAQQQQLVET QHQHVQKQHQ 670 680 690 700 710 720 SQPQVQQPPP QLLQDPSQQP LPIYHTMPPP QTSPVVVTSP VLLEQPPPQP MPVVQQQQTQ 730 740 750 760 770 780 QLATPKPEVS PAPPSSNTTT PTGIASTPTA GVIASAGSEK TTPAAPTPTS NSATVPTGTA 790 800 810 820 830 840 ATAGGATGTT PVVKKHVLNP SAKPFTPRGP STPNPSRPHT PQTPVPMTNI YTTTGGHVPP 850 860 870 880 890 900 AANQPIYVMQ PQHPFPPQTH PQAGQPPRLR RSNYPPMAAS QMHVSASAAT GQPLITAGPI 910 920 930 940 950 960 PQFIQYGHAP HQQQFQSHTY APMQMRVYPD QPQQLQFMTQ TPQSTTPSPG QPHQQFHPPP 970 980 990 1000 1010 1020 QPSPAGGGPQ PAFTPPTQAA TYQLMCVHPQ SLLANHYFPP PTPQHPQQNQ QQYQIVMQQH QPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B), RNA EDITING OF POSITIONS 381 AND 398. Tissue: Embryo, Head and Testis.
[4]	"A Drosophila homolog of the polyglutamine disease gene SCA2 is a dosage-sensitive regulator of actin filament formation." Satterfield T.F., Jackson S.M., Pallanck L.J. Genetics 162:1687-1702(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[5]	"RNA editing in Drosophila melanogaster: new targets and functional consequences." Stapleton M., Carlson J.W., Celniker S.E. RNA 12:1922-1932(2006) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING OF POSITIONS 381 AND 398.
[6]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, MASS SPECTROMETRY.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-208; SER-211 AND SER-221, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014297 Genomic DNA. Translation: AAN13663.1. AE014297 Genomic DNA. Translation: AAN13664.2. AE014297 Genomic DNA. Translation: AAF55196.2. AY058253 mRNA. Translation: AAL13482.1. AY069494 mRNA. Translation: AAL39639.1. Different initiation. AY095516 mRNA. Translation: AAM12250.1. BT001272 mRNA. Translation: AAN71028.1. Sequence problems. BT001340 mRNA. Translation: AAN71095.1. Sequence problems.
RefSeq	NP_650466.2. NM_142209.3. NP_732033.1. NM_169657.2. NP_732034.2. NM_169658.3.
UniGene	Dm.1489.
3D structure databases
ProteinModelPortal	Q8SWR8.
SMR	Q8SWR8. Positions 62-131.
ModBase	Search...
Proteomic databases
PaxDb	Q8SWR8.
PRIDE	Q8SWR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0083099; FBpp0082555; FBgn0041188.
GeneID	41883.
KEGG	dme:Dmel_CG5166.
UCSC	CG5166-RA. d. melanogaster.
Organism-specific databases
CTD	41883.
FlyBase	FBgn0041188. Atx2.
Phylogenomic databases
eggNOG	NOG268173.
GeneTree	ENSGT00530000063565.
InParanoid	Q8SWR8.
OMA	FMHSATA.
OrthoDB	EOG4F7M1J.
PhylomeDB	Q8SWR8.
Gene expression databases
Bgee	Q8SWR8.
Family and domain databases
InterPro	IPR009604. LsmAD_domain. IPR025852. SM_dom_ATX. [Graphical view]
Pfam	PF06741. LsmAD. 1 hit. PF14438. SM-ATX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ATXN2. drosophila.
GenomeRNAi	41883.
NextBio	826090.

Entry information

Entry name

ATX2_DROME

Accession

Primary (citable) accession number: Q8SWR8
Secondary accession number(s): Q8IHA4 , Q8IHF7, Q8INE0, Q8T081, Q95U80, Q9VF64

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project