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Q8SWL3

- ENP1_ENCCU

UniProt

Q8SWL3 - ENP1_ENCCU

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Protein

Spore wall and anchoring disk complex protein EnP1

Gene

EnP1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Spore wall protein involved in the adhesion to host cells surface glycoaminoglycans (GAGs). Microsporidian spore adherence is an integral part of activation and host cell invasion which requires the extrusion at the spore apex of a very long and coiled structure, the polar tube, through which the sporoplasm is pushed to enter into the potential host cell.1 Publication

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Sporulation

Names & Taxonomyi

Protein namesi
Recommended name:
Spore wall and anchoring disk complex protein EnP1
Alternative name(s):
Host cell adhesion protein EnP1
Gene namesi
Name:EnP1
Ordered Locus Names:ECU01_0820
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
ProteomesiUP000000819: Chromosome I

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU01_0820.

Subcellular locationi

Spore wall. Spore wallperispore
Note: Localizes also at the anchoring disk complex which consists of the polar sac and the anchoring disk, playing a crucial role in the rupture of the spore wall and the subsequent release of the polar tube following activation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 357341Spore wall and anchoring disk complex protein EnP1PRO_0000377523Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Developmental stagei

Expressed during merogony and a large part of sporogony.2 Publications

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi150 – 1589HBM1
Motifi329 – 3346HBM2

Domaini

Heparin-binding motifs (HBMs) are characterized by an XBBXBX or XBBBXXBX sequence, where X is any neutral amino acid and B is a positively charged basic amino acid, and are defined as the consensus sequence necessary for protein-heparin interactions. HBM1 motif is necessary for spore adherence to host cells.1 Publication

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG127634.
HOGENOMiHOG000112375.
InParanoidiQ8SWL3.
OrthoDBiEOG7TF7MF.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8SWL3 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLLGFLIVG LSAISALKTK ALHLTCEQEL RPYSAVVDAN CMAFALNGSN
60 70 80 90 100
IHEAIKYLQA MNIKKAYVLY WNDHDLRGTP MVLYDNGALA PFDPYTNTAK
110 120 130 140 150
YVLCVEACPC PGSKAASVGG FQAATSSEKI YVEGSARPAQ CSEVCIEPVE
160 170 180 190 200
RRPHYKKIVV NPSPSNCIPC EPECYDSSSS SECNKKRCKT FPRICKEKCG
210 220 230 240 250
SRRRGCPRKV EVLKSQKTYT FDIEKYRRRG EVVVRVCSKD SKEKFERFIL
260 270 280 290 300
SRNGEIRGNN NKNCILEPLP KCLRCPGQLH KLKKHIERKV CQEVCMYINA
310 320 330 340 350
KCDIFVLVGD CDFYRVVVND RRRYRNLHLK KVRGHKLREL IKHGLFGVEF

GPLDLDR
Length:357
Mass (Da):40,584
Last modified:June 1, 2002 - v1
Checksum:iABF93E135E9735B1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL391737 Genomic DNA. Translation: CAD24952.1.
RefSeqiXP_965917.1. XM_960824.1.

Genome annotation databases

GeneIDi860258.
KEGGiecu:ECU01_0820.

Cross-referencesi

Web resourcesi

Protein Spotlight

In like a shot - Issue 110 of October 2009

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL391737 Genomic DNA. Translation: CAD24952.1 .
RefSeqi XP_965917.1. XM_960824.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 860258.
KEGGi ecu:ECU01_0820.

Organism-specific databases

EuPathDBi MicrosporidiaDB:ECU01_0820.

Phylogenomic databases

eggNOGi NOG127634.
HOGENOMi HOG000112375.
InParanoidi Q8SWL3.
OrthoDBi EOG7TF7MF.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)."
    Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P.
    Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.
  3. "EnP1 and EnP2, two proteins associated with the Encephalitozoon cuniculi endospore, the chitin-rich inner layer of the microsporidian spore wall."
    Peuvel-Fanget I., Polonais V., Brosson D., Texier C., Kuhn L., Peyret P., Vivares C.P., Delbac F.
    Int. J. Parasitol. 36:309-318(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Expression of two cell wall proteins during the intracellular development of Encephalitozoon cuniculi: an immunocytochemical and in situ hybridization study with ultrathin frozen sections."
    Taupin V., Metenier G., Delbac F., Vivares C.P., Prensier G.
    Parasitology 132:815-825(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  5. "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
    Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
    Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.
  6. "EnP1, a microsporidian spore wall protein that enables spores to adhere to and infect host cells in vitro."
    Southern T.R., Jolly C.E., Lester M.E., Hayman J.R.
    Eukaryot. Cell 6:1354-1362(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN, SUBCELLULAR LOCATION, FUNCTION.

Entry informationi

Entry nameiENP1_ENCCU
AccessioniPrimary (citable) accession number: Q8SWL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

External Data

Dasty 3