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Q8SWL3

- ENP1_ENCCU

UniProt

Q8SWL3 - ENP1_ENCCU

Protein

Spore wall and anchoring disk complex protein EnP1

Gene

EnP1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Spore wall protein involved in the adhesion to host cells surface glycoaminoglycans (GAGs). Microsporidian spore adherence is an integral part of activation and host cell invasion which requires the extrusion at the spore apex of a very long and coiled structure, the polar tube, through which the sporoplasm is pushed to enter into the potential host cell.1 Publication

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Biological processi

    Cell adhesion, Sporulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Spore wall and anchoring disk complex protein EnP1
    Alternative name(s):
    Host cell adhesion protein EnP1
    Gene namesi
    Name:EnP1
    Ordered Locus Names:ECU01_0820
    OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
    Taxonomic identifieri284813 [NCBI]
    Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
    ProteomesiUP000000819: Chromosome I

    Organism-specific databases

    EuPathDBiMicrosporidiaDB:ECU01_0820.

    Subcellular locationi

    Spore wall. Spore wallperispore
    Note: Localizes also at the anchoring disk complex which consists of the polar sac and the anchoring disk, playing a crucial role in the rupture of the spore wall and the subsequent release of the polar tube following activation.

    GO - Cellular componenti

    1. spore wall Source: UniProtKB-SubCell

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 357341Spore wall and anchoring disk complex protein EnP1PRO_0000377523Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Developmental stagei

    Expressed during merogony and a large part of sporogony.2 Publications

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi150 – 1589HBM1
    Motifi329 – 3346HBM2

    Domaini

    Heparin-binding motifs (HBMs) are characterized by an XBBXBX or XBBBXXBX sequence, where X is any neutral amino acid and B is a positively charged basic amino acid, and are defined as the consensus sequence necessary for protein-heparin interactions. HBM1 motif is necessary for spore adherence to host cells.1 Publication

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG127634.
    HOGENOMiHOG000112375.
    OrthoDBiEOG7TF7MF.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8SWL3-1 [UniParc]FASTAAdd to Basket

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    MKLLGFLIVG LSAISALKTK ALHLTCEQEL RPYSAVVDAN CMAFALNGSN    50
    IHEAIKYLQA MNIKKAYVLY WNDHDLRGTP MVLYDNGALA PFDPYTNTAK 100
    YVLCVEACPC PGSKAASVGG FQAATSSEKI YVEGSARPAQ CSEVCIEPVE 150
    RRPHYKKIVV NPSPSNCIPC EPECYDSSSS SECNKKRCKT FPRICKEKCG 200
    SRRRGCPRKV EVLKSQKTYT FDIEKYRRRG EVVVRVCSKD SKEKFERFIL 250
    SRNGEIRGNN NKNCILEPLP KCLRCPGQLH KLKKHIERKV CQEVCMYINA 300
    KCDIFVLVGD CDFYRVVVND RRRYRNLHLK KVRGHKLREL IKHGLFGVEF 350
    GPLDLDR 357
    Length:357
    Mass (Da):40,584
    Last modified:June 1, 2002 - v1
    Checksum:iABF93E135E9735B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL391737 Genomic DNA. Translation: CAD24952.1.
    RefSeqiXP_965917.1. XM_960824.1.

    Genome annotation databases

    GeneIDi860258.
    KEGGiecu:ECU01_0820.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    In like a shot - Issue 110 of October 2009

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL391737 Genomic DNA. Translation: CAD24952.1 .
    RefSeqi XP_965917.1. XM_960824.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 860258.
    KEGGi ecu:ECU01_0820.

    Organism-specific databases

    EuPathDBi MicrosporidiaDB:ECU01_0820.

    Phylogenomic databases

    eggNOGi NOG127634.
    HOGENOMi HOG000112375.
    OrthoDBi EOG7TF7MF.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)."
      Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P.
      Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.
    3. "EnP1 and EnP2, two proteins associated with the Encephalitozoon cuniculi endospore, the chitin-rich inner layer of the microsporidian spore wall."
      Peuvel-Fanget I., Polonais V., Brosson D., Texier C., Kuhn L., Peyret P., Vivares C.P., Delbac F.
      Int. J. Parasitol. 36:309-318(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    4. "Expression of two cell wall proteins during the intracellular development of Encephalitozoon cuniculi: an immunocytochemical and in situ hybridization study with ultrathin frozen sections."
      Taupin V., Metenier G., Delbac F., Vivares C.P., Prensier G.
      Parasitology 132:815-825(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    5. "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
      Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
      Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.
    6. "EnP1, a microsporidian spore wall protein that enables spores to adhere to and infect host cells in vitro."
      Southern T.R., Jolly C.E., Lester M.E., Hayman J.R.
      Eukaryot. Cell 6:1354-1362(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN, SUBCELLULAR LOCATION, FUNCTION.

    Entry informationi

    Entry nameiENP1_ENCCU
    AccessioniPrimary (citable) accession number: Q8SWL3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

    External Data

    Dasty 3