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Q8SWL3 (ENP1_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spore wall and anchoring disk complex protein EnP1
Alternative name(s):
Host cell adhesion protein EnP1
Gene names
Name:EnP1
Ordered Locus Names:ECU01_0820
OrganismEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]
Taxonomic identifier284813 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Spore wall protein involved in the adhesion to host cells surface glycoaminoglycans (GAGs). Microsporidian spore adherence is an integral part of activation and host cell invasion which requires the extrusion at the spore apex of a very long and coiled structure, the polar tube, through which the sporoplasm is pushed to enter into the potential host cell. Ref.6

Subcellular location

Spore wall. Spore wallperispore. Note: Localizes also at the anchoring disk complex which consists of the polar sac and the anchoring disk, playing a crucial role in the rupture of the spore wall and the subsequent release of the polar tube following activation. Ref.3 Ref.4 Ref.6

Developmental stage

Expressed during merogony and a large part of sporogony. Ref.4

Domain

Heparin-binding motifs (HBMs) are characterized by an XBBXBX or XBBBXXBX sequence, where X is any neutral amino acid and B is a positively charged basic amino acid, and are defined as the consensus sequence necessary for protein-heparin interactions. HBM1 motif is necessary for spore adherence to host cells. Ref.6

Ontologies

Keywords
   Biological processCell adhesion
Sporulation
   DomainSignal
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

sporulation resulting in formation of a cellular spore

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentspore wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 357341Spore wall and anchoring disk complex protein EnP1
PRO_0000377523

Regions

Motif150 – 1589HBM1
Motif329 – 3346HBM2

Amino acid modifications

Glycosylation471N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8SWL3 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: ABF93E135E9735B1

FASTA35740,584
        10         20         30         40         50         60 
MKLLGFLIVG LSAISALKTK ALHLTCEQEL RPYSAVVDAN CMAFALNGSN IHEAIKYLQA 

        70         80         90        100        110        120 
MNIKKAYVLY WNDHDLRGTP MVLYDNGALA PFDPYTNTAK YVLCVEACPC PGSKAASVGG 

       130        140        150        160        170        180 
FQAATSSEKI YVEGSARPAQ CSEVCIEPVE RRPHYKKIVV NPSPSNCIPC EPECYDSSSS 

       190        200        210        220        230        240 
SECNKKRCKT FPRICKEKCG SRRRGCPRKV EVLKSQKTYT FDIEKYRRRG EVVVRVCSKD 

       250        260        270        280        290        300 
SKEKFERFIL SRNGEIRGNN NKNCILEPLP KCLRCPGQLH KLKKHIERKV CQEVCMYINA 

       310        320        330        340        350 
KCDIFVLVGD CDFYRVVVND RRRYRNLHLK KVRGHKLREL IKHGLFGVEF GPLDLDR 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)."
Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P.
Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.
[2]"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi."
Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P.
Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.
[3]"EnP1 and EnP2, two proteins associated with the Encephalitozoon cuniculi endospore, the chitin-rich inner layer of the microsporidian spore wall."
Peuvel-Fanget I., Polonais V., Brosson D., Texier C., Kuhn L., Peyret P., Vivares C.P., Delbac F.
Int. J. Parasitol. 36:309-318(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Expression of two cell wall proteins during the intracellular development of Encephalitozoon cuniculi: an immunocytochemical and in situ hybridization study with ultrathin frozen sections."
Taupin V., Metenier G., Delbac F., Vivares C.P., Prensier G.
Parasitology 132:815-825(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[5]"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.
[6]"EnP1, a microsporidian spore wall protein that enables spores to adhere to and infect host cells in vitro."
Southern T.R., Jolly C.E., Lester M.E., Hayman J.R.
Eukaryot. Cell 6:1354-1362(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN, SUBCELLULAR LOCATION, FUNCTION.

Web resources

Protein Spotlight

In like a shot - Issue 110 of October 2009

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL391737 Genomic DNA. Translation: CAD24952.1.
RefSeqXP_965917.1. XM_960824.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID860258.
KEGGecu:ECU01_0820.

Organism-specific databases

EuPathDBMicrosporidiaDB:ECU01_0820.

Phylogenomic databases

eggNOGNOG127634.
HOGENOMHOG000112375.
OrthoDBEOG7TF7MF.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameENP1_ENCCU
AccessionPrimary (citable) accession number: Q8SWL3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 1, 2002
Last modified: February 19, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries