Q8SWL3 (ENP1_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spore wall and anchoring disk complex protein EnP1

Alternative name(s):
Host cell adhesion protein EnP1

Gene names

Name:	EnP1
Ordered Locus Names:	ECU01_0820

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Spore wall protein involved in the adhesion to host cells surface glycoaminoglycans (GAGs). Microsporidian spore adherence is an integral part of activation and host cell invasion which requires the extrusion at the spore apex of a very long and coiled structure, the polar tube, through which the sporoplasm is pushed to enter into the potential host cell. Ref.6
Subcellular location	Spore wall. Spore wall › perispore. Note: Localizes also at the anchoring disk complex which consists of the polar sac and the anchoring disk, playing a crucial role in the rupture of the spore wall and the subsequent release of the polar tube following activation. Ref.3 Ref.4 Ref.6
Developmental stage	Expressed during merogony and a large part of sporogony. Ref.4
Domain	Heparin-binding motifs (HBMs) are characterized by an XBBXBX or XBBBXXBX sequence, where X is any neutral amino acid and B is a positively charged basic amino acid, and are defined as the consensus sequence necessary for protein-heparin interactions. HBM1 motif is necessary for spore adherence to host cells. Ref.6

Ontologies

Keywords
Biological process	Cell adhesion Sporulation
Domain	Signal
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	spore wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 357	341	Spore wall and anchoring disk complex protein EnP1		PRO_0000377523
Regions
	Motif	150 – 158	9	HBM1
	Motif	329 – 334	6	HBM2
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8SWL3 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: ABF93E135E9735B1	FASTA	357	40,584
	10 20 30 40 50 60 MKLLGFLIVG LSAISALKTK ALHLTCEQEL RPYSAVVDAN CMAFALNGSN IHEAIKYLQA 70 80 90 100 110 120 MNIKKAYVLY WNDHDLRGTP MVLYDNGALA PFDPYTNTAK YVLCVEACPC PGSKAASVGG 130 140 150 160 170 180 FQAATSSEKI YVEGSARPAQ CSEVCIEPVE RRPHYKKIVV NPSPSNCIPC EPECYDSSSS 190 200 210 220 230 240 SECNKKRCKT FPRICKEKCG SRRRGCPRKV EVLKSQKTYT FDIEKYRRRG EVVVRVCSKD 250 260 270 280 290 300 SKEKFERFIL SRNGEIRGNN NKNCILEPLP KCLRCPGQLH KLKKHIERKV CQEVCMYINA 310 320 330 340 350 KCDIFVLVGD CDFYRVVVND RRRYRNLHLK KVRGHKLREL IKHGLFGVEF GPLDLDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)." Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P. Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[2]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[3]	"EnP1 and EnP2, two proteins associated with the Encephalitozoon cuniculi endospore, the chitin-rich inner layer of the microsporidian spore wall." Peuvel-Fanget I., Polonais V., Brosson D., Texier C., Kuhn L., Peyret P., Vivares C.P., Delbac F. Int. J. Parasitol. 36:309-318(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Expression of two cell wall proteins during the intracellular development of Encephalitozoon cuniculi: an immunocytochemical and in situ hybridization study with ultrathin frozen sections." Taupin V., Metenier G., Delbac F., Vivares C.P., Prensier G. Parasitology 132:815-825(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[5]	"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages." Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C. Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.
[6]	"EnP1, a microsporidian spore wall protein that enables spores to adhere to and infect host cells in vitro." Southern T.R., Jolly C.E., Lester M.E., Hayman J.R. Eukaryot. Cell 6:1354-1362(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN, SUBCELLULAR LOCATION, FUNCTION.

Web resources

Protein Spotlight

In like a shot - Issue 110 of October 2009

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL391737 Genomic DNA. Translation: CAD24952.1.
RefSeq	XP_965917.1. XM_960824.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	860258.
KEGG	ecu:ECU01_0820.
Phylogenomic databases
eggNOG	NOG127634.
HOGENOM	HOG000112375.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ENP1_ENCCU

Accession

Primary (citable) accession number: Q8SWL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program