Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lysophospholipase NTE1

Gene

NTE1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by organophosphorus esters.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei643 – 6431By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi198 – 319122cNMP 1Add
BLAST
Nucleotide bindingi310 – 428119cNMP 2Add
BLAST

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. phosphatidylcholine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase NTE1 (EC:3.1.1.5)
Alternative name(s):
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene namesi
Name:NTE1
Ordered Locus Names:ECU05_0070
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
ProteomesiUP000000819 Componenti: Chromosome V

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU05_0070.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence AnalysisAdd
BLAST
Topological domaini22 – 905884CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 905905Lysophospholipase NTE1PRO_0000295321Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi6035.ECU05_0070.

Structurei

3D structure databases

ProteinModelPortaliQ8SVN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini610 – 774165PatatinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi641 – 6455GXSXG

Sequence similaritiesi

Belongs to the NTE family.Curated
Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 patatin domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000113869.
InParanoidiQ8SVN8.
KOiK14676.
OrthoDBiEOG70W3NS.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 3 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS01237. UPF0028. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8SVN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSLSLLIAI IVVTGLLAGF RHYGSSRCRT VPFKIKSRSI EKTASTRLLG
60 70 80 90 100
MSLEDAMKRY PLFNFYPGQS IREILSSWER VTYGNGETIA APGFILQGLV
110 120 130 140 150
EIWYREHLVC VKEKGSFLNG SMELLGYSTR GTKKARGSVE VLLIDASLVR
160 170 180 190 200
DNIWYILFSM LRKSCIEIAC RYFELESKLV EKETMKIKDD GQGHLDLFLS
210 220 230 240 250
FLNEKLLLNL DDGKEILRRD IEARLIGRGE TIKDSEESMD YIMYVVSGEI
260 270 280 290 300
LVLAGESAFV FGKGSVFGYF SLFFDLYSSI KIQAREDSSV LLCSSSVLLK
310 320 330 340 350
FGLDEKKFDH SMLLDIDESL HIIDESAEWM RLLPGDMIAQ KGDASKEIFY
360 370 380 390 400
IGAGSVKSAS RESSSGTVIG GKECIFGESW NESSIATRIT DVVRIPSLLV
410 420 430 440 450
DYFLERDKSF FKKYTKRLFE SGNKANGKIV SIIPVGQYKD IESFSRKLKS
460 470 480 490 500
AIGASSLLLS RRDVVEILAR RAFDTTEEVR FMDYLTKMSR RYEIILIYVE
510 520 530 540 550
NEYSRLLRYL LNFSDVLLAV GTTFADIPEY NVYCRIEFVK IYEERRASDE
560 570 580 590 600
RSVKKSKKIN HGPRYLESRK ESFGQYDRVH HVLFPSKTML FCSKDFQRLA
610 620 630 640 650
RSLLGKRIGL VLGGGGARGL AHIGVIQALE EEGIPIDCVG GTSMGAFIGA
660 670 680 690 700
LYAKECNNYH VFKQAKRFSR KMSNIWILLL DLTYPICSMF SGHAFNRSLH
710 720 730 740 750
SIFGDGLIQD LWLEYFCITT NIVSYEEEVH RNGMVWRYVR ASMGLCGYLP
760 770 780 790 800
PICDNKKLLV DGGYLNNVPA DVMMGMQVEK IITVDVGTVL ENDHFDYGDT
810 820 830 840 850
LSGFTILFNK FFGNKKFVTM EEIQYRLAYI STERKMKELE ANGNIIMLRP
860 870 880 890 900
DLGKYRTMDF KKFDEIVACG YQSTKNAIAK WKQEGTYDVL FFSLKKQTPK

RRYSV
Length:905
Mass (Da):103,088
Last modified:June 1, 2002 - v1
Checksum:i94B9D7775EA8E92C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590445 Genomic DNA. Translation: CAD26524.1.
RefSeqiNP_597347.1. NM_001041213.1.

Genome annotation databases

GeneIDi859011.
KEGGiecu:ECU05_0070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590445 Genomic DNA. Translation: CAD26524.1.
RefSeqiNP_597347.1. NM_001041213.1.

3D structure databases

ProteinModelPortaliQ8SVN8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6035.ECU05_0070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi859011.
KEGGiecu:ECU05_0070.

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU05_0070.

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000113869.
InParanoidiQ8SVN8.
KOiK14676.
OrthoDBiEOG70W3NS.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 3 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS01237. UPF0028. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.

Entry informationi

Entry nameiNTE1_ENCCU
AccessioniPrimary (citable) accession number: Q8SVN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: June 1, 2002
Last modified: January 7, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.