Q8SV03 (FCP1_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase II subunit A C-terminal domain phosphatase
EC=3.1.3.16

Alternative name(s):
CTD phosphatase FCP1

Gene names

Name:	FCP1
Ordered Locus Names:	ECU07_0890

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Ref.2
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Magnesium or manganese or cobalt By similarity.
Subunit structure	Monomer. Ref.2
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 1 BRCT domain. Contains 1 FCP1 homology domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	Cobalt Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	RNA polymerase II subunit A C-terminal domain phosphatase		PRO_0000212566
Regions
	Domain	56 – 212	157	FCP1 homology
	Domain	309 – 393	85	BRCT
Sites
	Active site	66	1	By similarity
	Active site	68	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8SV03 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 030806FD1C2C6221	FASTA	411	47,791
	10 20 30 40 50 60 MGGCNHPIRL GTLCGVCGME IQEESHLFCA LYNTDNVKIT HEEAVAIHKE KMEALEMQMK 70 80 90 100 110 120 LILVLDLDQT VLHTTYGTSS LEGTVKFVID RCRYCVKLRP NLDYMLRRIS KLYEIHVYTM 130 140 150 160 170 180 GTRAYAERIV EIIDPSGKYF DDRIITRDEN QGVLVKRLSR LFPHDHRNIV ILDDRPDVWD 190 200 210 220 230 240 YCENLVLIRP FWYFNRVDIN DPLRLKRKIE KEAGENKALE EFVSKRKKIE DIRNPEIASR 250 260 270 280 290 300 LDDMVLESSC GSEGVEDDSR STEEKEVSEV QSVASGDSEL LKVAGFLRKV HRKYFASKQR 310 320 330 340 350 360 NVKRILRKIR RRVFGGDRFF VAEIANRAWL VKTIEMYGGI VGIPESGVDF VVSSCEREAE 370 380 390 400 410 YLAQKFECLA VSPKWIADCV YSLKRVEYGK YVVCDHRTKD EYEEELERLF T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[2]	"An Encephalitozoon cuniculi ortholog of the RNA polymerase II carboxyl-terminal domain (CTD) serine phosphatase Fcp1." Hausmann S., Schwer B., Shuman S. Biochemistry 43:7111-7120(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590447 Genomic DNA. Translation: CAD25621.1.
RefSeq	NP_586017.1. NM_001041639.1.
3D structure databases
ProteinModelPortal	Q8SV03.
ModBase	Search...
Protein-protein interaction databases
STRING	6035.ECU07_0890.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	859447.
KEGG	ecu:ECU07_0890.
Phylogenomic databases
eggNOG	COG5190.
HOGENOM	HOG000112561.
KO	K15732.
OMA	CEREAEY.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR001357. BRCT_dom. IPR011947. FCP1_euk. IPR023214. HAD-like_dom. IPR004274. NIF. [Graphical view]
Pfam	PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. SM00577. CPDc. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR02250. FCP1_euk. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FCP1_ENCCU

Accession

Primary (citable) accession number: Q8SV03

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program