Q8ST66 (ABCGI_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ABC transporter G family member 18

Alternative name(s):
ABC transporter ABCG.18
ABC transporter mdrA2

Gene names

Name:	abcG18
Synonyms:	mdra2
ORF Names:	DDB_G0275687

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for endocytosis and endosomal pH regulation. Ref.1
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Disruption phenotype	Cells that have a abcG2-abcG18 disruption have an endocytosis rate roughly 70% that of wild-type (or rtoA disrupted cells). Disruption on abcG2-abcG18-rtoA cells have an endocytosis rate roughly 20% that of wild-type. The exocytosis rates of abcG2-abcG18 and abcG2-abcG18-rtoA disrupted cells are roughly that of wild-type; abcG2-abcG18 endosomes have an unusually high pH, whereas abcG2-abcG18-rtoA endosomes have an almost normal pH. Ref.1
Sequence similarities	Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily. [View classification] Contains 2 ABC transmembrane type-2 domains. Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Endocytosis Transport
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from genetic interaction Ref.1. Source: dictyBase endocytosis Inferred from genetic interaction Ref.1. Source: dictyBase endosomal lumen acidification Inferred from genetic interaction Ref.1. Source: dictyBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of substances Inferred from sequence or structural similarity Ref.1Ref.2. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1476	1476	ABC transporter G family member 18		PRO_0000330366
Regions
	Transmembrane	455 – 475	21	Helical; Potential
	Transmembrane	485 – 505	21	Helical; Potential
	Transmembrane	523 – 543	21	Helical; Potential
	Transmembrane	561 – 581	21	Helical; Potential
	Transmembrane	590 – 610	21	Helical; Potential
	Transmembrane	618 – 638	21	Helical; Potential
	Transmembrane	698 – 718	21	Helical; Potential
	Transmembrane	1211 – 1228	18	Helical; Potential
	Transmembrane	1239 – 1259	21	Helical; Potential
	Transmembrane	1290 – 1310	21	Helical; Potential
	Transmembrane	1324 – 1344	21	Helical; Potential
	Transmembrane	1349 – 1369	21	Helical; Potential
	Transmembrane	1448 – 1468	21	Helical; Potential
	Domain	69 – 312	244	ABC transporter 1
	Domain	445 – 722	278	ABC transmembrane type-2 1
	Domain	814 – 1053	240	ABC transporter 2
	Domain	1201 – 1471	271	ABC transmembrane type-2 2
	Nucleotide binding	105 – 112	8	ATP 1 Potential
	Nucleotide binding	848 – 855	8	ATP 2 Potential
	Compositional bias	366 – 369	4	Poly-Met
	Compositional bias	370 – 373	4	Poly-Asn
	Compositional bias	1089 – 1132	44	Asn-rich
	Compositional bias	1135 – 1143	9	Poly-Ser
Experimental info
	Sequence conflict	18	1	R → G in AAK69777. Ref.1
	Sequence conflict	1122	1	S → R in AAK69777. Ref.1
	Sequence conflict	1350	1	A → G in AAK69777. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8ST66 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D729985CE02821A7	FASTA	1,476	166,508
	10 20 30 40 50 60 MENEEKREYL AKSRSNSRNF LNDSIESKNI FGVDKKSEPN LKQSIDGGLN FSTTSSYNPS 70 80 90 100 110 120 NLPRTSVFVS ARNLSSTVGH GKNEKKILTD LNFFLKPGSM VLLLGSPGCG KTSLMNTLAL 130 140 150 160 170 180 LTSNEKITGN LLFNGKTGDP NTHHRHVSYV VQDDFHMAPL TVRDTFKFSA DCQSGDKSEK 190 200 210 220 230 240 ERIEIVDNVL DFLDLKHVQN TVVGDEFLRG ISGGQKKRVT IGVELVKESN LLLMDEPTNG 250 260 270 280 290 300 LDSSISLEML TKIKNKVQQE KMSCLISLLQ PGLEITKLFD YLMIMNQGQM SYFGPMNQAI 310 320 330 340 350 360 GYFEGLGFKF PKHHNPAEFF QEIVDEPELY CGIDDGSSDG GSGDSGSSSG GSNYNYNFKN 370 380 390 400 410 420 KASSTMMMMN NNNKIIPPLK GSDEFAMAYR KSIIYKHILE YIDSHIPDEE ERSKFIDYST 430 440 450 460 470 480 TLKPYSTGFG RQLSLNVKRG FKLFLGNKAS IRLRLLKNVI IGFILGTLYW KLDTTQADGS 490 500 510 520 530 540 NRSGLLFFSL LTFVFGGFGS ISVFFDQRQV FYSERAWKYY NTITYFLSMI VTDLPMSIVE 550 560 570 580 590 600 VLIFSNFVYW MTGLNKTWDR FIYFFLTCLV CDVMSLSIIR SVCSFTKTKY AASAISPAVV 610 620 630 640 650 660 SPFILMCGYM KHSNEIPGWW IWLYWISPIH YGFEGLLLNE HSGLDYHCSP DELMPPSFLP 670 680 690 700 710 720 TFNTSYPVGF EGNQVCPITK GEQILDSIGF HTEFYYRWVD LAIISAFTLL FWLITLVCMK 730 740 750 760 770 780 FLVFRVYRKD PVGIKKSKPN KTTTLIKMNR NSTDSTTTNN SMNYFNNKHN KKQNDDSDSG 790 800 810 820 830 840 EEMESVDVDV KSSGKANLRK DIPIGCYMQW KDLVYEVDVK KDGKNQRLRL LNGINGYVKP 850 860 870 880 890 900 GMLVALMGPS GAGKSTLLDV LANRKTGGHT KGQILINGQE RTKYFTRTSA YVEQMDILTP 910 920 930 940 950 960 VSTVREVILF SAKNRLPNSV PIQEKEEFVD NILETLNLLK IQHSLIGDVE SGLSLSQRKR 970 980 990 1000 1010 1020 VNMGIELASD PQLLFLDEPT SGLDSSAALK VMNLIKKIAS SGRSVICTIH QPSTTIFKKF 1030 1040 1050 1060 1070 1080 DHLLLLKRGG ETVYFGPTGT NSKIVLNYFA ERGLICDPFK NPADFILDVT EDIIEIIQPP 1090 1100 1110 1120 1130 1140 QTLPTSTSNE NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN TSDTLNNNNK KNLESSLSSS 1150 1160 1170 1180 1190 1200 SISFDPVESF KESKENQKLL SIVENSIMPV GTPVAVYHGK YSSTIKTQFI ELLKRSWKGG 1210 1220 1230 1240 1250 1260 IRRVDTIRTR VGRSFVLGLV IGTLFLRLDK EQNDVFNRIS FLFFSLMFGG MAGLSIIPTV 1270 1280 1290 1300 1310 1320 STERGVFYRE QASGMYRVWI YYLTFVLSDL PFVIITSYAY VIPVYFLTGL SLSNHGWDFF 1330 1340 1350 1360 1370 1380 YHSFISVMLY LNFGLTSIAF ATSLPVEEMA FLLNGVLLSV TSLFAGFMIP PPSMPAAWKW 1390 1400 1410 1420 1430 1440 AFYLDFISYP LKAFLITEFK DMEFVCTDNK GAIPIPIPSQ NTTKFFCPIT HGTQVLDRID 1450 1460 1470 YKISFQYWDI LIMASFTFAL LVGGYLSLKF IRYQNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ABC transporters required for endocytosis and endosomal pH regulation in Dictyostelium." Brazill D.T., Meyer L.R., Hatton R.D., Brock D.A., Gomer R.H. J. Cell Sci. 114:3923-3932(2001) [PubMed: 11719559] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, FUNCTION.
[2]	"Evolutionary analyses of ABC transporters of Dictyostelium discoideum." Anjard C., Loomis W.F. Eukaryot. Cell 1:643-652(2002) [PubMed: 12456012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], NOMENCLATURE. Strain: AX4.
[3]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF246689 Genomic DNA. Translation: AAK69777.1. AF482381 Genomic DNA. Translation: AAL91487.1. AAFI02000013 Genomic DNA. Translation: EAL69594.1.
RefSeq	XP_643502.1. XM_638410.1.
3D structure databases
ProteinModelPortal	Q8ST66.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185012; DDB0185012; DDB_G0275687.
GeneID	8620083.
GenomeReviews	Gene locus abcG18 in contig CM000151_GR.
KEGG	ddi:DDB_G0275687.
Organism-specific databases
dictyBase	DDB_G0275687. abcG18.
Phylogenomic databases
eggNOG	KOG0065.
GeneTree	EPrGT00050000001450.
HOGENOM	HBG081041.
OMA	WNAIASK.
PhylomeDB	Q8ST66.
Family and domain databases
InterPro	IPR013525. ABC_2_trans. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF01061. ABC2_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS51012. ABC_TM2. False negative. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABCGI_DICDI

Accession

Primary (citable) accession number: Q8ST66
Secondary accession number(s): Q553B8, Q8MXM5, Q965D3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	June 1, 2002
Last modified:	September 21, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)