Q8SSM8 (ALF_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase
EC=4.1.2.13

Gene names

Ordered Locus Names:

ECU01_0240

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Developmental stage	Expressed in late sporogonial stages.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 338

338

Fructose-bisphosphate aldolase

PRO_0000381751

Sites

Active site

179

1

Proton acceptor By similarity

Active site

221

1

Schiff-base intermediate with dihydroxyacetone-P By similarity

Binding site

50

1

Substrate By similarity

Binding site

138

1

Substrate By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

338

Helix Strand Turn

Details...

Helix

4 – 18

15

Helix

19 – 21

3

Beta strand

23 – 27

5

Helix

31 – 40

10

Helix

47 – 58

12

Helix

63 – 65

3

Beta strand

67 – 72

6

Helix

74 – 78

5

Helix

87 – 93

7

Beta strand

97 – 101

5

Beta strand

106 – 109

4

Turn

110 – 112

3

Beta strand

113 – 116

4

Helix

119 – 121

3

Helix

122 – 126

5

Helix

129 – 131

3

Beta strand

136 – 143

8

Helix

152 – 171

20

Beta strand

175 – 182

8

Beta strand

184 – 186

3

Helix

190 – 210

21

Helix

215 – 217

3

Beta strand

219 – 222

4

Helix

237 – 251

15

Beta strand

258 – 261

4

Helix

268 – 280

13

Beta strand

286 – 294

9

Helix

295 – 305

11

Helix

309 – 311

3

Helix

312 – 330

19

Sequences

	Sequence		Length	Mass (Da)
	Q8SSM8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2158A1DAE05A7AF9	FASTA	338	37,917
	10 20 30 40 50 60 MMDCDHLLRL GMTAKKILEN GKGILAADET PKTLGRRFEK LGITNTEENR RKFREILFST 70 80 90 100 110 120 KGIERYIGGV ILNQETFEQT SGSGVPLTEL LKKKGIEIGI KLDKGLIDYK EKEKISVGLE 130 140 150 160 170 180 DLDLRCKSSA FKDATFAKWR SLFYFYDGIP SEDCINENCS ILAKYAIICQ KNGLVPIVEP 190 200 210 220 230 240 EVFLEGDYSM KRSYEVTRQI LSTLMKYLNY ELVYIPGVLI KASYVTSGQL SNEKYTPKKV 250 260 270 280 290 300 ATFTLRALLS TIPCGIPGIV FLSGGHGSED AIGFLNAINM ERGCRTWSLS FSFARALTDG 310 320 330 VLETWRGDDS NIEEAQKILL ETSFKACRGA EGKLWDQE

References

[1]	"Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)." Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P. Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[2]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[3]	"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages." Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C. Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL391737 Genomic DNA. Translation: CAD24894.1.

RefSeq

XP_965859.1. XM_960766.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3MBD	X-ray	2.00	A	1-338	[»]
3MBF	X-ray	2.37	A	1-338	[»]
3QRH	X-ray	2.00	A	1-338	[»]

ProteinModelPortal

Q8SSM8.

ModBase

Search...

Protein-protein interaction databases

STRING

6035.ECU01_0240.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

860198.

KEGG

ecu:ECU01_0240.

Phylogenomic databases

eggNOG

COG3588.

HOGENOM

HOG000220876.

KO

K01623.

OMA

IDNQCPS.

Enzyme and pathway databases

UniPathway

UPA00109; UER00183.

Family and domain databases

Gene3D

3.20.20.70. 1 hit.

InterPro

IPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]

PANTHER

PTHR11627. PTHR11627. 1 hit.

Pfam

PF00274. Glycolytic. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8SSM8.

Entry information

Entry name

ALF_ENCCU

Accession

Primary (citable) accession number: Q8SSM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program