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Protein

Fructose-bisphosphate aldolase

Gene

ECU01_0240

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Probable glucose-6-phosphate isomerase (ECU05_0650)
  3. 6-phosphofructokinase (ECU03_0680)
  4. Fructose-bisphosphate aldolase (ECU01_0240)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50SubstrateBy similarity1
Binding sitei138SubstrateBy similarity1
Active sitei179Proton acceptorBy similarity1
Active sitei221Schiff-base intermediate with dihydroxyacetone-PBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BRENDAi4.1.2.13. 7412.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Gene namesi
Ordered Locus Names:ECU01_0240
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000000819 Componenti: Chromosome I

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU01_0240.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003817511 – 338Fructose-bisphosphate aldolaseAdd BLAST338

Proteomic databases

PRIDEiQ8SSM8.

Expressioni

Developmental stagei

Expressed in late sporogonial stages.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi284813.XP_965859.1.

Structurei

Secondary structure

1338
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Helixi19 – 21Combined sources3
Beta strandi23 – 27Combined sources5
Helixi31 – 40Combined sources10
Helixi47 – 58Combined sources12
Helixi63 – 65Combined sources3
Beta strandi67 – 72Combined sources6
Helixi74 – 78Combined sources5
Helixi87 – 93Combined sources7
Beta strandi97 – 101Combined sources5
Beta strandi106 – 109Combined sources4
Turni110 – 112Combined sources3
Beta strandi113 – 116Combined sources4
Helixi119 – 121Combined sources3
Helixi122 – 126Combined sources5
Helixi129 – 131Combined sources3
Beta strandi136 – 143Combined sources8
Helixi152 – 171Combined sources20
Beta strandi175 – 182Combined sources8
Beta strandi184 – 186Combined sources3
Helixi190 – 210Combined sources21
Helixi215 – 217Combined sources3
Beta strandi219 – 222Combined sources4
Helixi237 – 251Combined sources15
Beta strandi258 – 261Combined sources4
Helixi268 – 280Combined sources13
Beta strandi286 – 294Combined sources9
Helixi295 – 305Combined sources11
Helixi309 – 311Combined sources3
Helixi312 – 330Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MBDX-ray2.00A1-338[»]
3MBFX-ray2.37A1-338[»]
3QRHX-ray2.00A1-338[»]
ProteinModelPortaliQ8SSM8.
SMRiQ8SSM8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8SSM8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
InParanoidiQ8SSM8.
KOiK01623.
OMAiANCQAAQ.
OrthoDBiEOG092C3GNG.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8SSM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMDCDHLLRL GMTAKKILEN GKGILAADET PKTLGRRFEK LGITNTEENR
60 70 80 90 100
RKFREILFST KGIERYIGGV ILNQETFEQT SGSGVPLTEL LKKKGIEIGI
110 120 130 140 150
KLDKGLIDYK EKEKISVGLE DLDLRCKSSA FKDATFAKWR SLFYFYDGIP
160 170 180 190 200
SEDCINENCS ILAKYAIICQ KNGLVPIVEP EVFLEGDYSM KRSYEVTRQI
210 220 230 240 250
LSTLMKYLNY ELVYIPGVLI KASYVTSGQL SNEKYTPKKV ATFTLRALLS
260 270 280 290 300
TIPCGIPGIV FLSGGHGSED AIGFLNAINM ERGCRTWSLS FSFARALTDG
310 320 330
VLETWRGDDS NIEEAQKILL ETSFKACRGA EGKLWDQE
Length:338
Mass (Da):37,917
Last modified:June 1, 2002 - v1
Checksum:i2158A1DAE05A7AF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391737 Genomic DNA. Translation: CAD24894.1.
RefSeqiXP_965859.1. XM_960766.1.

Genome annotation databases

EnsemblFungiiCAD24894; CAD24894; CAD24894.
GeneIDi860198.
KEGGiecu:ECU01_0240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391737 Genomic DNA. Translation: CAD24894.1.
RefSeqiXP_965859.1. XM_960766.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MBDX-ray2.00A1-338[»]
3MBFX-ray2.37A1-338[»]
3QRHX-ray2.00A1-338[»]
ProteinModelPortaliQ8SSM8.
SMRiQ8SSM8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284813.XP_965859.1.

Proteomic databases

PRIDEiQ8SSM8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAD24894; CAD24894; CAD24894.
GeneIDi860198.
KEGGiecu:ECU01_0240.

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU01_0240.

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
InParanoidiQ8SSM8.
KOiK01623.
OMAiANCQAAQ.
OrthoDBiEOG092C3GNG.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BRENDAi4.1.2.13. 7412.

Miscellaneous databases

EvolutionaryTraceiQ8SSM8.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALF_ENCCU
AccessioniPrimary (citable) accession number: Q8SSM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.