Q8SSL7 (AGM1_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phosphoacetylglucosamine mutase
Short name=PAGM
EC=5.4.2.3

Alternative name(s):
Acetylglucosamine phosphomutase
N-acetylglucosamine-phosphate mutase

Gene names

Name:	PCM1
Ordered Locus Names:	ECU01_0650

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interconverts GlcNAc-6-P and GlcNAc-1-P By similarity.
Catalytic activity	N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.
Developmental stage	Expressed in late sporogonial stages.
Sequence similarities	Belongs to the phosphohexose mutase family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UDP-N-acetylglucosamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: InterPro phosphoacetylglucosamine mutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Probable phosphoacetylglucosamine mutase		PRO_0000381747
Sites
	Active site	62	1	Phosphoserine intermediate By similarity
	Metal binding	62	1	Magnesium; via phosphate group By similarity
	Metal binding	278	1	Magnesium By similarity
	Metal binding	280	1	Magnesium By similarity
	Metal binding	282	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8SSL7 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: E74F296E1F93C1B6	FASTA	530	58,611
	10 20 30 40 50 60 MDNALIADEN LKKPSKPAYY GTAGYRSKTS DLNNILCRAS LIAYLRSTTF AGKIIGVMIT 70 80 90 100 110 120 ASHNPVEYNG IKIIDHNGDM LDEVWEEYSD RIVNCDDEKL AREMKKILRS CSNQSELGEG 130 140 150 160 170 180 VRGHVVLGRD TRDSGERLCN NIRSVLGKLN CTVDDYGVVT TPELHFLVRK CNTENRVVDK 190 200 210 220 230 240 AEYMKNIAHN FNSLSSITKG NLRMMIDTAN GVADMKLKEL DGMLDGKLNY EVLNDPKGIL 250 260 270 280 290 300 NLDCGADFVK TKKRAPRLEA LSSSGFSQAA NRICASFDGD VDRLIFFTGP KDTEIFDGDS 310 320 330 340 350 360 QAVFLALYIR SLLDRIESRL SIGVVLSYYS NNAAVDVLPP ESFKVVMAQT GVKNFVSAAR 370 380 390 400 410 420 EFDVGIYFEP NGHGSVCFSQ ACIDEIEKGS TKSHAILKIL ANLFDPCIGD ALANFVIFKA 430 440 450 460 470 480 LMGSADDLRK FRENPSRLLT VKIVDKNSIK VDQKNQVIEP KELQDKIDVE ALSLGGRSFV 490 500 510 520 530 RPSGTEDVVR VYAECPSEAD ADLLCLKVAQ HVYDMCNGIG DHPEIDYTSK

References

[1]	"Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)." Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P. Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[2]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[3]	"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages." Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C. Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL391737 Genomic DNA. Translation: CAD24935.1.
RefSeq	XP_965900.1. XM_960807.1.
3D structure databases
HSSP	HSSP built from PDB template 2DKA based on UniProtKB Q9P4V2.
ProteinModelPortal	Q8SSL7.
ModBase	Search...
Protein-protein interaction databases
STRING	6035.ECU01_0650.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	860240.
KEGG	ecu:ECU01_0650.
Phylogenomic databases
eggNOG	COG1109.
HOGENOM	HOG000210027.
KO	K01836.
OMA	DIVRVYA.
Enzyme and pathway databases
UniPathway	UPA00113; UER00530.
Family and domain databases
Gene3D	3.40.120.10. 2 hits.
InterPro	IPR005844. A-D-PHexomutase_a/b/a-I. IPR016055. A-D-PHexomutase_a/b/a-I/II/III. IPR005845. A-D-PHexomutase_a/b/a-II. IPR005843. A-D-PHexomutase_C. IPR016066. A-D-PHexomutase_CS. IPR016657. PAGM. [Graphical view]
PANTHER	PTHR22573:SF3. PTHR22573:SF3. 1 hit.
Pfam	PF02878. PGM_PMM_I. 2 hits. PF02879. PGM_PMM_II. 1 hit. PF00408. PGM_PMM_IV. 1 hit. [Graphical view]
PIRSF	PIRSF016408. PAGM. 1 hit.
SUPFAM	SSF53738. A-D-PHexomutase_a/b/a-I/II/III. 4 hits.
PROSITE	PS00710. PGM_PMM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGM1_ENCCU

Accession

Primary (citable) accession number: Q8SSL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program