Q8SSL2 (TPS1_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
EC=2.4.1.15

Alternative name(s):
General glucose sensor subunit 1
Trehalose synthase complex catalytic subunit TPS1
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase

Gene names

Name:	TPS1
Ordered Locus Names:	ECU01_0800

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Synthase catalytic subunit of the trehalose synthase complex that catalyzes the production of trehalose from glucose-6-phosphate and UDP-glucose in a two step process. Can function independently of the complex By similarity.
Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Subunit structure	Component of the trehalose synthase complex By similarity.
Subcellular location	Cytoplasm By similarity.
Developmental stage	Expressed in late sporogonial stages.
Sequence similarities	Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Alpha,alpha-trehalose-phosphate synthase [UDP-forming]		PRO_0000381753

Sequences

	Sequence		Length	Mass (Da)
	Q8SSL2 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 4691E0E97F948AA3	FASTA	459	52,575
	10 20 30 40 50 60 MKLLVVSNRL PLTVKKSKDG FEYTKTSGGL VTGLRGISDK IRFMWLGNIS GVELDEEEKK 70 80 90 100 110 120 VIRKDCWEKF HSIPVFIDPV LNSNSYDGFC NAILWPIIHS FKDDVAFTIK DYNAYVEYNT 130 140 150 160 170 180 IFCEEICKIV EDGDIVWVHD YHLMILPEML RKKSDKSFKI MFFLHAQFPP AEIMETLACR 190 200 210 220 230 240 REIVSGMAHS DLIAFHSFDY AINFDDTCRA NKVEVRSKLD AIPIGIDPAM FRSALKEEKT 250 260 270 280 290 300 VERIKELREM FRGRKILLGV DRTDYIKGMP HRVKGFQRFL EKHPEFLDNV VFLQVGVPSR 310 320 330 340 350 360 TSVKEYSSYI TKMNELVSET NSKFGSIESV HLYFLNKSVD FNELCALYAV SDMLLVTSLQ 370 380 390 400 410 420 DGMNLVALEY ISCQNENNGV LLLSSNAGAS TTLPAAVEVN SWNTEEIADG IHRAITMSLE 430 440 450 ERTERHEINR KAVDTFTSVE WAEKNLDGLC DDWRESLML

References

[1]	"Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)." Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P. Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[2]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[3]	"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages." Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C. Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL391737 Genomic DNA. Translation: CAD24950.1.
RefSeq	XP_965915.1. XM_960822.1.
3D structure databases
HSSP	HSSP built from PDB template 1GZ5 based on UniProtKB P31677.
ProteinModelPortal	Q8SSL2.
ModBase	Search...
Protein-protein interaction databases
STRING	6035.ECU01_0800.
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	860256.
KEGG	ecu:ECU01_0800.
Phylogenomic databases
eggNOG	COG0380.
HOGENOM	HOG000191477.
KO	K00697.
OMA	AAIPQHE.
Family and domain databases
InterPro	IPR001830. Glyco_trans_20. [Graphical view]
Pfam	PF00982. Glyco_transf_20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPS1_ENCCU

Accession

Primary (citable) accession number: Q8SSL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program