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Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

ECU02_1210

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei162 – 1621ATPBy similarity
Binding sitei188 – 1881GlutamateBy similarity
Binding sitei344 – 3441GlutamateBy similarity
Binding sitei347 – 3471ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi382 – 3865ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Ordered Locus Names:ECU02_1210
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
ProteomesiUP000000819 Componenti: Chromosome II

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU02_1210.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 642642Probable glutamate--tRNA ligase, cytoplasmicPRO_0000388391Add
BLAST

Proteomic databases

PRIDEiQ8SSE4.

Interactioni

Protein-protein interaction databases

STRINGi6035.ECU02_1210.

Structurei

3D structure databases

ProteinModelPortaliQ8SSE4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 1543Glutamate bindingBy similarity
Regioni326 – 3305Glutamate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi157 – 16610"HIGH" regionBy similarity
Motifi382 – 3865"KMSKS" regionBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
InParanoidiQ8SSE4.
KOiK01885.
OMAiSEDEIMW.
OrthoDBiEOG7MKWFN.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8SSE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGKSEFKEE LINYILLKKY GKGAQDPPVY SNALKKAPQE MFPPGLVDAL
60 70 80 90 100
DSYSINLSRS EGVEFLVLLN SLVNDIRSEE VKDIIFGMIN TNQMLTKLMK
110 120 130 140 150
DKKEVERFPD TCKMYSEQFK ANKPLLKEFN AGSRKEQGNL EIGEPSENVV
160 170 180 190 200
TRFPPEPNGR LHIGHARAAL LNWYFASKGN GRLLVRFDDT NPEKEEERFE
210 220 230 240 250
RGILSDLSLL GINEYTLSHT SDYFDKIIDL GVFLIGEGKA YADNTPQEVM
260 270 280 290 300
RDERGRGVES RCRSMDVEES KRIFKEMARG NASGYCLRAK IDMSSSNKAM
310 320 330 340 350
RDPVIFRVNE SPHHRTGDKY KVYPTYDFAC PIVDSLEGIT LSLRANEYRD
360 370 380 390 400
RNQQYYWFID NLRLRNRPKI HDFSRLNFEN TVLSKRKLKY YVDNGFVSGW
410 420 430 440 450
DDPRLATIAG IKRLGMNMEA LREYILMQGV SQKTCTISWD KVWAINRKKI
460 470 480 490 500
DPVSARYFCV QQRDAVEVSI DNTSEYTMDV PKHKKNGDLG TKEVFYSSQI
510 520 530 540 550
LLSQEDGRVL QDNEEFTLMN WGNAIVKSKT VENGTVTKME VSLNPDGDFK
560 570 580 590 600
LTKNKMSWVS KRGSVTVELA EYGNLMNDED TEDLALRFNR NSVKKEYWYA
610 620 630 640
ESAIINVREG EVIQFERNGF YYCDGFLVFN LLPFTKQKRT GN
Length:642
Mass (Da):73,964
Last modified:June 1, 2002 - v1
Checksum:iB2BC6D4B2C329BB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590442 Genomic DNA. Translation: CAD25150.1.
RefSeqiNP_584646.1. NM_001040835.1.

Genome annotation databases

GeneIDi858636.
KEGGiecu:ECU02_1210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590442 Genomic DNA. Translation: CAD25150.1.
RefSeqiNP_584646.1. NM_001040835.1.

3D structure databases

ProteinModelPortaliQ8SSE4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6035.ECU02_1210.

Proteomic databases

PRIDEiQ8SSE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi858636.
KEGGiecu:ECU02_1210.

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU02_1210.

Phylogenomic databases

eggNOGiCOG0008.
InParanoidiQ8SSE4.
KOiK01885.
OMAiSEDEIMW.
OrthoDBiEOG7MKWFN.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.

Entry informationi

Entry nameiSYEC_ENCCU
AccessioniPrimary (citable) accession number: Q8SSE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: June 1, 2002
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.