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Q8SSE4

- SYEC_ENCCU

UniProt

Q8SSE4 - SYEC_ENCCU

Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

ECU02_1210

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei162 – 1621ATPBy similarity
    Binding sitei188 – 1881GlutamateBy similarity
    Binding sitei344 – 3441GlutamateBy similarity
    Binding sitei347 – 3471ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi382 – 3865ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
    Alternative name(s):
    Glutamyl-tRNA synthetase
    Short name:
    GluRS
    Gene namesi
    Ordered Locus Names:ECU02_1210
    OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
    Taxonomic identifieri284813 [NCBI]
    Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
    ProteomesiUP000000819: Chromosome II

    Organism-specific databases

    EuPathDBiMicrosporidiaDB:ECU02_1210.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 642642Probable glutamate--tRNA ligase, cytoplasmicPRO_0000388391Add
    BLAST

    Proteomic databases

    PRIDEiQ8SSE4.

    Interactioni

    Protein-protein interaction databases

    STRINGi6035.ECU02_1210.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8SSE4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni152 – 1543Glutamate bindingBy similarity
    Regioni326 – 3305Glutamate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi157 – 16610"HIGH" regionBy similarity
    Motifi382 – 3865"KMSKS" regionBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0008.
    KOiK01885.
    OMAiLRLWEEM.
    OrthoDBiEOG7MKWFN.

    Family and domain databases

    Gene3Di1.10.1160.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR004526. Glu-tRNA-synth_arc/euk.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF50715. SSF50715. 1 hit.
    TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8SSE4-1 [UniParc]FASTAAdd to Basket

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    MDGKSEFKEE LINYILLKKY GKGAQDPPVY SNALKKAPQE MFPPGLVDAL    50
    DSYSINLSRS EGVEFLVLLN SLVNDIRSEE VKDIIFGMIN TNQMLTKLMK 100
    DKKEVERFPD TCKMYSEQFK ANKPLLKEFN AGSRKEQGNL EIGEPSENVV 150
    TRFPPEPNGR LHIGHARAAL LNWYFASKGN GRLLVRFDDT NPEKEEERFE 200
    RGILSDLSLL GINEYTLSHT SDYFDKIIDL GVFLIGEGKA YADNTPQEVM 250
    RDERGRGVES RCRSMDVEES KRIFKEMARG NASGYCLRAK IDMSSSNKAM 300
    RDPVIFRVNE SPHHRTGDKY KVYPTYDFAC PIVDSLEGIT LSLRANEYRD 350
    RNQQYYWFID NLRLRNRPKI HDFSRLNFEN TVLSKRKLKY YVDNGFVSGW 400
    DDPRLATIAG IKRLGMNMEA LREYILMQGV SQKTCTISWD KVWAINRKKI 450
    DPVSARYFCV QQRDAVEVSI DNTSEYTMDV PKHKKNGDLG TKEVFYSSQI 500
    LLSQEDGRVL QDNEEFTLMN WGNAIVKSKT VENGTVTKME VSLNPDGDFK 550
    LTKNKMSWVS KRGSVTVELA EYGNLMNDED TEDLALRFNR NSVKKEYWYA 600
    ESAIINVREG EVIQFERNGF YYCDGFLVFN LLPFTKQKRT GN 642
    Length:642
    Mass (Da):73,964
    Last modified:June 1, 2002 - v1
    Checksum:iB2BC6D4B2C329BB2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590442 Genomic DNA. Translation: CAD25150.1.
    RefSeqiNP_584646.1. NM_001040835.1.

    Genome annotation databases

    GeneIDi858636.
    KEGGiecu:ECU02_1210.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590442 Genomic DNA. Translation: CAD25150.1 .
    RefSeqi NP_584646.1. NM_001040835.1.

    3D structure databases

    ProteinModelPortali Q8SSE4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6035.ECU02_1210.

    Proteomic databases

    PRIDEi Q8SSE4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 858636.
    KEGGi ecu:ECU02_1210.

    Organism-specific databases

    EuPathDBi MicrosporidiaDB:ECU02_1210.

    Phylogenomic databases

    eggNOGi COG0008.
    KOi K01885.
    OMAi LRLWEEM.
    OrthoDBi EOG7MKWFN.

    Family and domain databases

    Gene3Di 1.10.1160.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR004526. Glu-tRNA-synth_arc/euk.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF50715. SSF50715. 1 hit.
    TIGRFAMsi TIGR00463. gltX_arch. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.

    Entry informationi

    Entry nameiSYEC_ENCCU
    AccessioniPrimary (citable) accession number: Q8SSE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3