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Protein

Inosine triphosphate pyrophosphatase

Gene

ECU04_1180

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn2+.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Magnesium or manganeseUniRule annotation
Binding sitei46 – 461SubstrateUniRule annotation
Metal bindingi64 – 641Magnesium or manganeseUniRule annotation
Binding sitei164 – 1641SubstrateUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nucleoside-triphosphate diphosphatase activity Source: UniProtKB-HAMAP
  3. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. deoxyribonucleoside triphosphate catabolic process Source: UniProtKB-HAMAP
  2. nucleotide metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine triphosphate pyrophosphataseUniRule annotation (EC:3.6.1.19UniRule annotation)
Short name:
ITPaseUniRule annotation
Short name:
Inosine triphosphataseUniRule annotation
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:ECU04_1180
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
ProteomesiUP000000819: Chromosome IV

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU04_1180.

Subcellular locationi

Cytoplasm UniRule annotation. Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 192192Inosine triphosphate pyrophosphatasePRO_0000413139Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi6035.ECU04_1180.

Structurei

3D structure databases

ProteinModelPortaliQ8SS24.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 136Substrate bindingUniRule annotation
Regioni64 – 652Substrate bindingUniRule annotation
Regioni169 – 1702Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0127.
HOGENOMiHOG000293320.
InParanoidiQ8SS24.
KOiK01519.
OMAiVCRGALP.
OrthoDBiEOG74R22R.

Family and domain databases

Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_03148. HAM1_NTPase.
InterProiIPR002637. Ham1p-like.
IPR027502. ITPase.
IPR029001. ITPase-like_fam.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8SS24-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRIYFATTN LKKLKEIRSL FEADIVHMNI PMVEIQASLE RIVDHKLNQV
60 70 80 90 100
VPCIGEGDAV IVDDTAVAFE GLYGFPGVYI KDFLRIGSRK ISEIVGKIGN
110 120 130 140 150
SNATAFCCLG IAHYRDGRVV KKVFFGELEG SIVESKEDGL EGFDYIFLPS
160 170 180 190
GSSMCLGDMP VDEKNRISHR RIASKKLADY MASVGIIKAH GS
Length:192
Mass (Da):21,149
Last modified:June 1, 2002 - v1
Checksum:iF6BA42A8B3B40F82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590444 Genomic DNA. Translation: CAD25306.1.
RefSeqiNP_584802.1. NM_001041152.1.

Genome annotation databases

GeneIDi858950.
KEGGiecu:ECU04_1180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590444 Genomic DNA. Translation: CAD25306.1.
RefSeqiNP_584802.1. NM_001041152.1.

3D structure databases

ProteinModelPortaliQ8SS24.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6035.ECU04_1180.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi858950.
KEGGiecu:ECU04_1180.

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU04_1180.

Phylogenomic databases

eggNOGiCOG0127.
HOGENOMiHOG000293320.
InParanoidiQ8SS24.
KOiK01519.
OMAiVCRGALP.
OrthoDBiEOG74R22R.

Family and domain databases

Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_03148. HAM1_NTPase.
InterProiIPR002637. Ham1p-like.
IPR027502. ITPase.
IPR029001. ITPase-like_fam.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.

Entry informationi

Entry nameiITPA_ENCCU
AccessioniPrimary (citable) accession number: Q8SS24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: June 1, 2002
Last modified: January 7, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.