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Q8SRU3

- PSA5_ENCCU

UniProt

Q8SRU3 - PSA5_ENCCU

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Protein

Probable proteasome subunit alpha type-5

Gene

PUP2

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Probable proteasome subunit alpha type-5 (EC:3.4.25.1)
Alternative name(s):
26S proteasome alpha-type subunit PUP2
Multicatalytic endopeptidase complex subunit PUP2
Gene namesi
Name:PUP2
Ordered Locus Names:ECU05_1400
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
ProteomesiUP000000819: Chromosome V

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU05_1400.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. proteasome core complex, alpha-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Probable proteasome subunit alpha type-5PRO_0000382756Add
BLAST

Expressioni

Developmental stagei

Expressed in late sporogonial stages.1 Publication

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).By similarity

Protein-protein interaction databases

STRINGi6035.ECU05_1400.

Structurei

3D structure databases

ProteinModelPortaliQ8SRU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091085.
InParanoidiQ8SRU3.
KOiK02729.
OMAiNVEMATI.
OrthoDBiEOG7SBP0C.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8SRU3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDFKQSVNT YSSEGRIHQI EYAMKAMNLG TTTIGVRTKE FVILCSEKKI
60 70 80 90 100
LSTLQNPKSV VKHYKIYDHI VLGFSGISGD TKTIVKRSRD FCISHTHMYG
110 120 130 140 150
ENISVERLLK YLSSLSLRFG EEDEAKMIFR RPFGVSLIIA GFDTEPRLYS
160 170 180 190 200
LDPSGSYISY KAKAIGSGHE VVEGILENEY EEYSGLDQSL RKVLCTLSKV
210 220 230
MKDKISKDNV EVVVVTQEES KFLTPEEVSV YL
Length:232
Mass (Da):26,291
Last modified:June 1, 2002 - v1
Checksum:i9F8E7CC6EDBECF13
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590445 Genomic DNA. Translation: CAD26660.1.
RefSeqiNP_597483.1. NM_001041349.1.

Genome annotation databases

GeneIDi859150.
KEGGiecu:ECU05_1400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590445 Genomic DNA. Translation: CAD26660.1 .
RefSeqi NP_597483.1. NM_001041349.1.

3D structure databases

ProteinModelPortali Q8SRU3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6035.ECU05_1400.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 859150.
KEGGi ecu:ECU05_1400.

Organism-specific databases

EuPathDBi MicrosporidiaDB:ECU05_1400.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000091085.
InParanoidi Q8SRU3.
KOi K02729.
OMAi NVEMATI.
OrthoDBi EOG7SBP0C.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view ]
SMARTi SM00948. Proteasome_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.
  2. "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
    Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
    Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiPSA5_ENCCU
AccessioniPrimary (citable) accession number: Q8SRU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3