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Q8SRS8

- SYLC_ENCCU

UniProt

Q8SRS8 - SYLC_ENCCU

Protein

Probable leucine--tRNA ligase, cytoplasmic

Gene

ECU06_0280

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei547 – 5471ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. leucine-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. tRNA aminoacylation for protein translation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase
    Short name:
    LeuRS
    Gene namesi
    Ordered Locus Names:ECU06_0280
    OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
    Taxonomic identifieri284813 [NCBI]
    Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
    ProteomesiUP000000819: Chromosome VI

    Organism-specific databases

    EuPathDBiMicrosporidiaDB:ECU06_0280.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 874874Probable leucine--tRNA ligase, cytoplasmicPRO_0000388413Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi6035.ECU06_0280.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8SRS8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi36 – 4611"HIGH" regionBy similarityAdd
    BLAST
    Motifi544 – 5485"KMSKS" regionBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000149408.
    KOiK01869.
    OMAiYSKFVEW.
    OrthoDBiEOG7TTQH2.

    Family and domain databases

    Gene3Di3.40.50.620. 2 hits.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PfamiPF00133. tRNA-synt_1. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8SRS8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEERSKLRYL SMLEVERDTI SDVSEKRKFF VTFTYPYMNG RLHLGHLFSI    50
    SKADFFSYYK ELQGYNVLFP FSFHCTGMPI SASAKKLAEE LSGEKVDLSV 100
    KKIIEDLGFD DVKPFTDPVH WVRTFPGLCE RSLKRFHGNI DWRRSFITTD 150
    INKYYDSFIK WQFNRLNELG HLSFGKRHSI FCPVDKQPCL DHDRRKGENV 200
    KPVGVVLCKL RFSEGILLAR IKQGCVPSKA VVGSRCDFIG FEYCNEKYFA 250
    EKDVFENVDA QASGICVRES VKGDFFGGRR FSGFGKEVVC DAIEKDVPCV 300
    VKGTQDKKDP SLAEYIKNEI ELISKVESTE TQLAETKDLL KFYEPEEEVI 350
    SRSGGKCIVA LTDQWYINYC DPEWKKKVKR CIENLVCTDD TRAILEDGLE 400
    WIGKWGFSRS FGLGTRIPWD SEYLIDSLSD STIYMAMYTF KHFLYRDLEG 450
    KDELFPSNRL SDDVWNYIFL NRSITEDLAP YEEILSNCRE SFNYFYPIDL 500
    RVGGKDLLKN HLIFFLFNHV ALFEEKHWPK RMFTNGHLML NSEKMSKSSG 550
    NYMTVDESLD KFGVSSTRMC LAVCGDGNED ANFVESNANA FVLKLYSYVK 600
    MIEELCTGRS LNPCILDLMK GYGEMGFADR FLMQTISMNV AHATRAHEDM 650
    TYRDVVKYGF YEMVHAKEMY HILGGTNNEI LFLLCKAMTQ LLYPIIPSLA 700
    RYLIETYFYS NFSLPVPFLS DTAEIDGVSY LKNTLKRLVA QKRRKKRCEV 750
    VEILVGVEYS EWKRKCMSII DQIACECKVL NINVSEEMKT GESQFVPKII 800
    DAVREVLKEF GIPEKKGILF SMDYLNHPEN YSVKFNEYEV LKAHKYYIEN 850
    NTGLEVIVCV SPRADPGTPL FEFK 874
    Length:874
    Mass (Da):101,082
    Last modified:June 1, 2002 - v1
    Checksum:i2021A5BAFA798E90
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590446 Genomic DNA. Translation: CAD25388.1.
    RefSeqiNP_585784.1. NM_001041406.1.

    Genome annotation databases

    GeneIDi859207.
    KEGGiecu:ECU06_0280.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590446 Genomic DNA. Translation: CAD25388.1 .
    RefSeqi NP_585784.1. NM_001041406.1.

    3D structure databases

    ProteinModelPortali Q8SRS8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6035.ECU06_0280.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 859207.
    KEGGi ecu:ECU06_0280.

    Organism-specific databases

    EuPathDBi MicrosporidiaDB:ECU06_0280.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000149408.
    KOi K01869.
    OMAi YSKFVEW.
    OrthoDBi EOG7TTQH2.

    Family and domain databases

    Gene3Di 3.40.50.620. 2 hits.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    Pfami PF00133. tRNA-synt_1. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.

    Entry informationi

    Entry nameiSYLC_ENCCU
    AccessioniPrimary (citable) accession number: Q8SRS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3