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Q8SRS8 (SYLC_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable leucine--tRNA ligase, cytoplasmic

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Ordered Locus Names:ECU06_0280
OrganismEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]
Taxonomic identifier284813 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon

Protein attributes

Sequence length874 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA aminoacylation for protein translation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 874874Probable leucine--tRNA ligase, cytoplasmic
PRO_0000388413

Regions

Motif36 – 4611"HIGH" region By similarity
Motif544 – 5485"KMSKS" region By similarity

Sites

Binding site5471ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8SRS8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 2021A5BAFA798E90

FASTA874101,082
        10         20         30         40         50         60 
MEERSKLRYL SMLEVERDTI SDVSEKRKFF VTFTYPYMNG RLHLGHLFSI SKADFFSYYK 

        70         80         90        100        110        120 
ELQGYNVLFP FSFHCTGMPI SASAKKLAEE LSGEKVDLSV KKIIEDLGFD DVKPFTDPVH 

       130        140        150        160        170        180 
WVRTFPGLCE RSLKRFHGNI DWRRSFITTD INKYYDSFIK WQFNRLNELG HLSFGKRHSI 

       190        200        210        220        230        240 
FCPVDKQPCL DHDRRKGENV KPVGVVLCKL RFSEGILLAR IKQGCVPSKA VVGSRCDFIG 

       250        260        270        280        290        300 
FEYCNEKYFA EKDVFENVDA QASGICVRES VKGDFFGGRR FSGFGKEVVC DAIEKDVPCV 

       310        320        330        340        350        360 
VKGTQDKKDP SLAEYIKNEI ELISKVESTE TQLAETKDLL KFYEPEEEVI SRSGGKCIVA 

       370        380        390        400        410        420 
LTDQWYINYC DPEWKKKVKR CIENLVCTDD TRAILEDGLE WIGKWGFSRS FGLGTRIPWD 

       430        440        450        460        470        480 
SEYLIDSLSD STIYMAMYTF KHFLYRDLEG KDELFPSNRL SDDVWNYIFL NRSITEDLAP 

       490        500        510        520        530        540 
YEEILSNCRE SFNYFYPIDL RVGGKDLLKN HLIFFLFNHV ALFEEKHWPK RMFTNGHLML 

       550        560        570        580        590        600 
NSEKMSKSSG NYMTVDESLD KFGVSSTRMC LAVCGDGNED ANFVESNANA FVLKLYSYVK 

       610        620        630        640        650        660 
MIEELCTGRS LNPCILDLMK GYGEMGFADR FLMQTISMNV AHATRAHEDM TYRDVVKYGF 

       670        680        690        700        710        720 
YEMVHAKEMY HILGGTNNEI LFLLCKAMTQ LLYPIIPSLA RYLIETYFYS NFSLPVPFLS 

       730        740        750        760        770        780 
DTAEIDGVSY LKNTLKRLVA QKRRKKRCEV VEILVGVEYS EWKRKCMSII DQIACECKVL 

       790        800        810        820        830        840 
NINVSEEMKT GESQFVPKII DAVREVLKEF GIPEKKGILF SMDYLNHPEN YSVKFNEYEV 

       850        860        870 
LKAHKYYIEN NTGLEVIVCV SPRADPGTPL FEFK 

« Hide

References

[1]"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi."
Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P.
Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590446 Genomic DNA. Translation: CAD25388.1.
RefSeqNP_585784.1. NM_001041406.1.

3D structure databases

ProteinModelPortalQ8SRS8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6035.ECU06_0280.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID859207.
KEGGecu:ECU06_0280.

Organism-specific databases

EuPathDBMicrosporidiaDB:ECU06_0280.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000149408.
KOK01869.
OMAMRINDAT.
OrthoDBEOG7TTQH2.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYLC_ENCCU
AccessionPrimary (citable) accession number: Q8SRS8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: June 1, 2002
Last modified: November 13, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries