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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

DUT1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.By similarity

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei133 – 1331SubstrateBy similarity

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dUMP biosynthetic process Source: UniProtKB-UniPathway
  2. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:DUT1
Ordered Locus Names:ECU06_0430
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
ProteomesiUP000000819 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU06_0430.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 144144Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000182928Add
BLAST

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi6035.ECU06_0430.

Structurei

3D structure databases

ProteinModelPortaliQ8SRS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 673Substrate bindingBy similarity
Regioni79 – 824Substrate bindingBy similarity
Regioni138 – 1392Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

eggNOGiCOG0756.
HOGENOMiHOG000028966.
InParanoidiQ8SRS0.
KOiK01520.
OMAiICERISY.
OrthoDBiEOG7T7H6C.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8SRS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVSEIRVRK INPRAKIPKR QSEGAAGYDI HSVESGRIPP NGRKSVRTGL
60 70 80 90 100
AWDVPQSVVG LVFGRSGLAL RNWIEVVETC VHPGEDKELV ITLVNNGREV
110 120 130 140
FEYEESSRIA QLVFVPVLSC EIDLVESLDL TERGCLGFGS TGMK
Length:144
Mass (Da):15,850
Last modified:June 1, 2002 - v1
Checksum:i9921FE374F5413FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590446 Genomic DNA. Translation: CAD25403.1.
RefSeqiNP_585799.1. NM_001041421.1.

Genome annotation databases

GeneIDi859223.
KEGGiecu:ECU06_0430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590446 Genomic DNA. Translation: CAD25403.1.
RefSeqiNP_585799.1. NM_001041421.1.

3D structure databases

ProteinModelPortaliQ8SRS0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6035.ECU06_0430.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi859223.
KEGGiecu:ECU06_0430.

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU06_0430.

Phylogenomic databases

eggNOGiCOG0756.
HOGENOMiHOG000028966.
InParanoidiQ8SRS0.
KOiK01520.
OMAiICERISY.
OrthoDBiEOG7T7H6C.

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.

Entry informationi

Entry nameiDUT_ENCCU
AccessioniPrimary (citable) accession number: Q8SRS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: June 1, 2002
Last modified: January 7, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.