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Q8SRS0 (DUT_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase

Short name=dUTPase
EC=3.6.1.23
Alternative name(s):
dUTP pyrophosphatase
Gene names
Name:DUT1
Ordered Locus Names:ECU06_0430
OrganismEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]
Taxonomic identifier284813 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon

Protein attributes

Sequence length144 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.

Catalytic activity

dUTP + H2O = dUMP + diphosphate.

Cofactor

Magnesium By similarity.

Pathway

Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.

Subunit structure

Homotrimer By similarity.

Sequence similarities

Belongs to the dUTPase family.

Ontologies

Keywords
   Biological processNucleotide metabolism
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdUMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

dUTP metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functiondUTP diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 144144Deoxyuridine 5'-triphosphate nucleotidohydrolase
PRO_0000182928

Regions

Region65 – 673Substrate binding By similarity
Region79 – 824Substrate binding By similarity
Region138 – 1392Substrate binding By similarity

Sites

Binding site1331Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8SRS0 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 9921FE374F5413FC

FASTA14415,850
        10         20         30         40         50         60 
MTVSEIRVRK INPRAKIPKR QSEGAAGYDI HSVESGRIPP NGRKSVRTGL AWDVPQSVVG 

        70         80         90        100        110        120 
LVFGRSGLAL RNWIEVVETC VHPGEDKELV ITLVNNGREV FEYEESSRIA QLVFVPVLSC 

       130        140 
EIDLVESLDL TERGCLGFGS TGMK 

« Hide

References

[1]"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi."
Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P.
Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590446 Genomic DNA. Translation: CAD25403.1.
RefSeqNP_585799.1. NM_001041421.1.

3D structure databases

ProteinModelPortalQ8SRS0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6035.ECU06_0430.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID859223.
KEGGecu:ECU06_0430.

Organism-specific databases

EuPathDBMicrosporidiaDB:ECU06_0430.

Phylogenomic databases

eggNOGCOG0756.
HOGENOMHOG000028966.
KOK01520.
OMALICEWIF.
OrthoDBEOG7T7H6C.

Enzyme and pathway databases

UniPathwayUPA00610; UER00666.

Family and domain databases

InterProIPR008180. dUTP_pyroPase.
IPR008181. dUTP_pyroPase_sf.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00576. dut. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDUT_ENCCU
AccessionPrimary (citable) accession number: Q8SRS0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: June 1, 2002
Last modified: November 13, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways