Q8SRR2 (RIR2_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase small chain
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small subunit

Gene names

Ordered Locus Names:

ECU06_0730

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small subunit By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Ribonucleoside-diphosphate reductase small chain		PRO_0000190461
Sites
	Active site	114	1	By similarity
	Metal binding	76	1	Iron 1 By similarity
	Metal binding	107	1	Iron 1 By similarity
	Metal binding	107	1	Iron 2 By similarity
	Metal binding	110	1	Iron 1 By similarity
	Metal binding	170	1	Iron 2 By similarity
	Metal binding	204	1	Iron 2 By similarity
	Metal binding	207	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8SRR2 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 79814EA716494886	FASTA	325	37,987
	10 20 30 40 50 60 MSHGEETELL LDPKEERFVL LPIKYHDIWK MYKKAESSFW TVEEVSLDKD IDDWGKLNAK 70 80 90 100 110 120 ERHFISYVLA FFAASDGIVN LNLVERFSTE VKVLEARFFY GFQMAIENIH SEMYSLLIDT 130 140 150 160 170 180 YIRDNDEKNF LFDAIRTIPS VKEKADWAIR WIEDKNSDFA TRLVAFACVE GIFFSGAFAS 190 200 210 220 230 240 IFWLKKRGLM PGLTFSNELI SRDEGLHCEF ACLLHYHLKK KCNRIKEVVM DAVEIEKKFL 250 260 270 280 290 300 SESLPVNLIG MNCNLMCRYI EFVADRLLEN LGEERVYNAT NPFDFMENIS LVGKTNFFDK 310 320 RESQYQKAFV GIENGNDSFR IDVDF

References

[1]

"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590446 Genomic DNA. Translation: CAD25433.1.
RefSeq	NP_585829.1. NM_001041451.1.
3D structure databases
ProteinModelPortal	Q8SRR2.
SMR	Q8SRR2. Positions 6-287.
ModBase	Search...
Protein-protein interaction databases
STRING	6035.ECU06_0730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	859253.
KEGG	ecu:ECU06_0730.
Phylogenomic databases
eggNOG	COG0208.
HOGENOM	HOG000255975.
KO	K10808.
OMA	MIASADS.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_ENCCU

Accession

Primary (citable) accession number: Q8SRR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program