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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

GFA1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (GFA1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene namesi
Name:GFA1
Ordered Locus Names:ECU07_1280
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000000819 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU07_1280

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003817452 – 699Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST698

Proteomic databases

PRIDEiQ8SRI2

Expressioni

Developmental stagei

Expressed in late sporogonial stages.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi284813.NP_586057.1

Structurei

3D structure databases

ProteinModelPortaliQ8SRI2
SMRiQ8SRI2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 303Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST302
Domaini377 – 516SIS 1PROSITE-ProRule annotationAdd BLAST140
Domaini544 – 689SIS 2PROSITE-ProRule annotationAdd BLAST146

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiKOG1268 Eukaryota
COG0449 LUCA
HOGENOMiHOG000258898
InParanoidiQ8SRI2
KOiK00820
OMAiASEYRYA
OrthoDBiEOG092C0ST1

Family and domain databases

CDDicd05008 SIS_GlmS_GlmD_1, 1 hit
cd05009 SIS_GlmS_GlmD_2, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR035466 GlmS/AgaS_SIS
IPR035490 GlmS/FrlB_SIS
IPR005855 GlmS_trans
IPR029055 Ntn_hydrolases_N
IPR001347 SIS
PfamiView protein in Pfam
PF01380 SIS, 2 hits
SUPFAMiSSF56235 SSF56235, 2 hits
TIGRFAMsiTIGR01135 glmS, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS51464 SIS, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8SRI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFGYANF SKERSKDEIA NIMINGLKRI EYRGYDSAGF CITDNTDRNF
60 70 80 90 100
ARIRAVGKVN SLYEIKNSQT SVDLTRKVLN HVSIAHTRWA THGQPSIENS
110 120 130 140 150
HPLSSDENNS FLVVHNGIIT NYKDLKVYLK KKGFTFESDT DTECAAKLAL
160 170 180 190 200
YFYREMERKK EETDFVAIVK NVVKHCEGAF AFVFASSLFP NELVTVRKSS
210 220 230 240 250
PVLIGLKPSG KMSFDFFGVN YGDPADDTPT SLLDSPLAFS KSDRHGFDQN
260 270 280 290 300
IDMMTRDVQD CTLHAHNKEP LEVFVASDAS ALIEHTRKVI FLEDNDIVHI
310 320 330 340 350
SNGNISIHRM HSKAKESGPE IREVKTIETE LAAIMKGNYD HYMIKEINEQ
360 370 380 390 400
EESVVNTMRG RINFESLTVS LGGLKDHVSG IRKSQRIIFV ACGTSYHASL
410 420 430 440 450
ANRALLEELL EIPVSVEIAS DFLDRAPPIM RSDCVFFVSQ SGETADSVMA
460 470 480 490 500
LRYCMSMGAL CVGITNTVGS TISRETACGV HINAGPEIGV ASTKAYTSQY
510 520 530 540 550
IALVLVALQL SDQNLVKQAR RREIMEGLKN ISSQINRVLE LSTSVKSLAN
560 570 580 590 600
GPMKDDASLL LIGRGYQYPT CMEGALKIKE ITYIHAEGLA AGELKHGPIA
610 620 630 640 650
LVDDKLRIIF IATKDLLYDK TRNAMEQIFA RGGRPIVICT EDISGDYAEY
660 670 680 690
DTFVVPKTVD CLQGILTVIP LQLLSYHLAV AKGYNADFPR NLAKSVTVE
Length:699
Mass (Da):77,544
Last modified:June 1, 2002 - v1
Checksum:i225F2F74C19C6B36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590447 Genomic DNA Translation: CAD25661.1
RefSeqiNP_586057.1, NM_001041679.1

Genome annotation databases

EnsemblFungiiCAD25661; CAD25661; CAD25661
GeneIDi859487
KEGGiecu:ECU07_1280

Similar proteinsi

Entry informationi

Entry nameiGFA1_ENCCU
AccessioniPrimary (citable) accession number: Q8SRI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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