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Q8SRI2

- GFA1_ENCCU

UniProt

Q8SRI2 - GFA1_ENCCU

Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

GFA1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).By similarity

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate biosynthetic process Source: InterPro
    2. glutamine metabolic process Source: UniProtKB-KW
    3. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00113; UER00528.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
    Short name:
    GFAT
    Alternative name(s):
    D-fructose-6-phosphate amidotransferase
    Hexosephosphate aminotransferase
    Gene namesi
    Name:GFA1
    Ordered Locus Names:ECU07_1280
    OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
    Taxonomic identifieri284813 [NCBI]
    Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
    ProteomesiUP000000819: Chromosome VII

    Organism-specific databases

    EuPathDBiMicrosporidiaDB:ECU07_1280.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 699698Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000381745Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed in late sporogonial stages.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi6035.ECU07_1280.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8SRI2.
    SMRiQ8SRI2. Positions 339-687.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 303302Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini377 – 516140SIS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini544 – 689146SIS 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
    Contains 2 SIS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Phylogenomic databases

    eggNOGiCOG0449.
    HOGENOMiHOG000258898.
    KOiK00820.
    OMAiSAGICTL.
    OrthoDBiEOG7034RG.

    Family and domain databases

    Gene3Di3.60.20.10. 2 hits.
    InterProiIPR017932. GATase_2_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
    PfamiPF01380. SIS. 2 hits.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 2 hits.
    TIGRFAMsiTIGR01135. glmS. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8SRI2-1 [UniParc]FASTAAdd to Basket

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    MCGIFGYANF SKERSKDEIA NIMINGLKRI EYRGYDSAGF CITDNTDRNF    50
    ARIRAVGKVN SLYEIKNSQT SVDLTRKVLN HVSIAHTRWA THGQPSIENS 100
    HPLSSDENNS FLVVHNGIIT NYKDLKVYLK KKGFTFESDT DTECAAKLAL 150
    YFYREMERKK EETDFVAIVK NVVKHCEGAF AFVFASSLFP NELVTVRKSS 200
    PVLIGLKPSG KMSFDFFGVN YGDPADDTPT SLLDSPLAFS KSDRHGFDQN 250
    IDMMTRDVQD CTLHAHNKEP LEVFVASDAS ALIEHTRKVI FLEDNDIVHI 300
    SNGNISIHRM HSKAKESGPE IREVKTIETE LAAIMKGNYD HYMIKEINEQ 350
    EESVVNTMRG RINFESLTVS LGGLKDHVSG IRKSQRIIFV ACGTSYHASL 400
    ANRALLEELL EIPVSVEIAS DFLDRAPPIM RSDCVFFVSQ SGETADSVMA 450
    LRYCMSMGAL CVGITNTVGS TISRETACGV HINAGPEIGV ASTKAYTSQY 500
    IALVLVALQL SDQNLVKQAR RREIMEGLKN ISSQINRVLE LSTSVKSLAN 550
    GPMKDDASLL LIGRGYQYPT CMEGALKIKE ITYIHAEGLA AGELKHGPIA 600
    LVDDKLRIIF IATKDLLYDK TRNAMEQIFA RGGRPIVICT EDISGDYAEY 650
    DTFVVPKTVD CLQGILTVIP LQLLSYHLAV AKGYNADFPR NLAKSVTVE 699
    Length:699
    Mass (Da):77,544
    Last modified:June 1, 2002 - v1
    Checksum:i225F2F74C19C6B36
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590447 Genomic DNA. Translation: CAD25661.1.
    RefSeqiNP_586057.1. NM_001041679.1.

    Genome annotation databases

    GeneIDi859487.
    KEGGiecu:ECU07_1280.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590447 Genomic DNA. Translation: CAD25661.1 .
    RefSeqi NP_586057.1. NM_001041679.1.

    3D structure databases

    ProteinModelPortali Q8SRI2.
    SMRi Q8SRI2. Positions 339-687.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6035.ECU07_1280.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 859487.
    KEGGi ecu:ECU07_1280.

    Organism-specific databases

    EuPathDBi MicrosporidiaDB:ECU07_1280.

    Phylogenomic databases

    eggNOGi COG0449.
    HOGENOMi HOG000258898.
    KOi K00820.
    OMAi SAGICTL.
    OrthoDBi EOG7034RG.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00528 .

    Family and domain databases

    Gene3Di 3.60.20.10. 2 hits.
    InterProi IPR017932. GATase_2_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
    Pfami PF01380. SIS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 2 hits.
    TIGRFAMsi TIGR01135. glmS. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.
    2. "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
      Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
      Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiGFA1_ENCCU
    AccessioniPrimary (citable) accession number: Q8SRI2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3