Q8SRD8 (SYR_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Ordered Locus Names:

ECU08_0550

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
Subunit structure	Monomer By similarity.
Developmental stage	Expressed in late sporogonial stages.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 563	563	Arginine--tRNA ligase		PRO_0000383142

Sequences

	Sequence		Length	Mass (Da)
	Q8SRD8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F6E50A60D4D4BB50	FASTA	563	64,253
	10 20 30 40 50 60 MFNELFRKVV EAISKASKFT PEEIAACMER SYQPKKPNVT LFLDRISPSP QEDAKELLET 70 80 90 100 110 120 LAGANIELIE NLAIRKSSVC CDINKRAILK DVLGYIQKNR EIFGNNNVGK GKRMVVEYSS 130 140 150 160 170 180 PNIAKIFHIG HLRTTVLGQF IVNLLRASGY ETTSINYFGD WGKQFGFVLL GYSKYGSEEE 190 200 210 220 230 240 LEKDPLKHLF NVYVKISADA EKNPDVDSEA KEIFRMMEED KDEWCMNLWR RFRELSIEKY 250 260 270 280 290 300 KVLYKRLNVE FDVYSGESMY NEKGKSIVET SKQIKTDEDG SKVFDLGKAG KVLVMKNDGT 310 320 330 340 350 360 TLYITRDIAA AIERLEEYSP EKIIYVVSSE QNKHFEDLFG VLEMLGYDKD KFQHVSYGLV 370 380 390 400 410 420 AGMSTRAGKV QLLEDIIQES TEVMKNVMMS DNNKGSFTAA EMDQTAEVLA ISTLLVMDFT 430 440 450 460 470 480 ARRVKGYEFD IEKRARNTSG TGLYLQYAHC RLRSIETKNS NVDYNDIETI DFELIHVPKV 490 500 510 520 530 540 LNLVYKLLWF EHVVEKCLED YEPSRIVTYL QDLASSINGA INILRVLGVD KELARARLLV 550 560 LSSARIVLHN GLRILGATPL NKM

References

[1]

"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.

[2]

"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages." Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C. Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590448 Genomic DNA. Translation: CAD26360.1.
RefSeq	NP_597184.1. NM_001041793.1.
3D structure databases
HSSP	HSSP built from PDB template 1BS2 based on UniProtKB Q05506.
ProteinModelPortal	Q8SRD8.
ModBase	Search...
Protein-protein interaction databases
STRING	6035.ECU08_0550.
Proteomic databases
PRIDE	Q8SRD8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	859606.
KEGG	ecu:ECU08_0550.
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247211.
KO	K01887.
OMA	IHFDEYS.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_ENCCU

Accession

Primary (citable) accession number: Q8SRD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program