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Q8SRB8

- NTH_ENCCU

UniProt

Q8SRB8 - NTH_ENCCU

Protein

Endonuclease III homolog

Gene

NTH1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.UniRule annotation

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei149 – 1491Nucleophile; for N-glycosylase activityUniRule annotation
    Sitei168 – 1681Important for catalytic activityUniRule annotation
    Metal bindingi217 – 2171Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi224 – 2241Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi227 – 2271Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi233 – 2331Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
    3. hydrolase activity, acting on glycosyl bonds Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. base-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease III homologUniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
    Alternative name(s):
    Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
    Short name:
    DNA glycoslyase/AP lyaseUniRule annotation
    Gene namesi
    Name:NTH1UniRule annotation
    Ordered Locus Names:ECU08_0880
    OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
    Taxonomic identifieri284813 [NCBI]
    Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
    ProteomesiUP000000819: Chromosome VIII

    Organism-specific databases

    EuPathDBiMicrosporidiaDB:ECU08_0880.

    Subcellular locationi

    Nucleus UniRule annotation. Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238Endonuclease III homologPRO_0000382919Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed in late sporogonial stages.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi6035.ECU08_0880.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8SRB8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini129 – 15527HhHUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Nth/MutY family.UniRule annotation
    Contains 1 HhH domain.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0177.
    HOGENOMiHOG000252209.
    KOiK10773.
    OMAiHINECIK.
    OrthoDBiEOG7PK99N.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPiMF_03183. Endonuclease_III_Nth.
    InterProiIPR011257. DNA_glycosylase.
    IPR004035. Endouclease-III_FeS-bd_BS.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR023170. HTH_base_excis_C.
    IPR005759. Nth.
    [Graphical view]
    PfamiPF00730. HhH-GPD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001435. Nth. 1 hit.
    SMARTiSM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    [Graphical view]
    SUPFAMiSSF48150. SSF48150. 1 hit.
    PROSITEiPS00764. ENDONUCLEASE_III_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8SRB8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSASGEERE GPLGLYLEIK MQRKDIVSPV DTMGCSITPS CRTEEERRFH    50
    ILVSLLLSSQ TKDEVTYEAM ARLRKLLPES AATDGEARGG LTIERVANSD 100
    VKHINECIKK VGFHNRKAAN LKKIAEILRE KGLPREMKDL ISLPGIGNKM 150
    ALLYMSHACN RTVGISVDTH VHRISNRIGL VRTRDVESTR RELERVVPRK 200
    EWKTINNILV GFGQTICVAK RPRCEECCIR GRCPSSLF 238
    Length:238
    Mass (Da):26,755
    Last modified:June 1, 2002 - v1
    Checksum:i3D9D7B774187F35B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590448 Genomic DNA. Translation: CAD26394.1.
    RefSeqiNP_597218.1. NM_001041827.1.

    Genome annotation databases

    GeneIDi859640.
    KEGGiecu:ECU08_0880.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590448 Genomic DNA. Translation: CAD26394.1 .
    RefSeqi NP_597218.1. NM_001041827.1.

    3D structure databases

    ProteinModelPortali Q8SRB8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6035.ECU08_0880.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 859640.
    KEGGi ecu:ECU08_0880.

    Organism-specific databases

    EuPathDBi MicrosporidiaDB:ECU08_0880.

    Phylogenomic databases

    eggNOGi COG0177.
    HOGENOMi HOG000252209.
    KOi K10773.
    OMAi HINECIK.
    OrthoDBi EOG7PK99N.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPi MF_03183. Endonuclease_III_Nth.
    InterProi IPR011257. DNA_glycosylase.
    IPR004035. Endouclease-III_FeS-bd_BS.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR023170. HTH_base_excis_C.
    IPR005759. Nth.
    [Graphical view ]
    Pfami PF00730. HhH-GPD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001435. Nth. 1 hit.
    SMARTi SM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48150. SSF48150. 1 hit.
    PROSITEi PS00764. ENDONUCLEASE_III_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.
    2. "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
      Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
      Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiNTH_ENCCU
    AccessioniPrimary (citable) accession number: Q8SRB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3