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Protein

Endonuclease III homolog

Gene

NTH1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei149Nucleophile; for N-glycosylase activityUniRule annotation1
Sitei168Important for catalytic activityUniRule annotation1
Metal bindingi217Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi224Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi227Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi233Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase, Lyase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease III homologUniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
Alternative name(s):
Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Short name:
DNA glycosylase/AP lyaseUniRule annotation
Gene namesi
Name:NTH1UniRule annotation
Ordered Locus Names:ECU08_0880
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000000819 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU08_0880

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003829191 – 238Endonuclease III homologAdd BLAST238

Expressioni

Developmental stagei

Expressed in late sporogonial stages.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi284813.NP_597218.1

Structurei

3D structure databases

ProteinModelPortaliQ8SRB8
SMRiQ8SRB8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini129 – 155HhHUniRule annotationAdd BLAST27

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1921 Eukaryota
COG0177 LUCA
HOGENOMiHOG000252209
InParanoidiQ8SRB8
KOiK10773
OMAiKAKNPLC
OrthoDBiEOG092C3TX5

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 1 hit
HAMAPiMF_03183 Endonuclease_III_Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR023170 HTH_base_excis_C
IPR030841 NTH1
PfamiView protein in Pfam
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q8SRB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSASGEERE GPLGLYLEIK MQRKDIVSPV DTMGCSITPS CRTEEERRFH
60 70 80 90 100
ILVSLLLSSQ TKDEVTYEAM ARLRKLLPES AATDGEARGG LTIERVANSD
110 120 130 140 150
VKHINECIKK VGFHNRKAAN LKKIAEILRE KGLPREMKDL ISLPGIGNKM
160 170 180 190 200
ALLYMSHACN RTVGISVDTH VHRISNRIGL VRTRDVESTR RELERVVPRK
210 220 230
EWKTINNILV GFGQTICVAK RPRCEECCIR GRCPSSLF
Length:238
Mass (Da):26,755
Last modified:June 1, 2002 - v1
Checksum:i3D9D7B774187F35B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590448 Genomic DNA Translation: CAD26394.1
RefSeqiNP_597218.1, NM_001041827.1

Genome annotation databases

EnsemblFungiiCAD26394; CAD26394; CAD26394
GeneIDi859640
KEGGiecu:ECU08_0880

Similar proteinsi

Entry informationi

Entry nameiNTH_ENCCU
AccessioniPrimary (citable) accession number: Q8SRB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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