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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

ZWF1

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (ZWF1)
  2. no protein annotated in this organism
  3. 6-phosphogluconate dehydrogenase, decarboxylating (ECU05_0860)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NADPBy similarity1
Binding sitei112NADP; via carbonyl oxygenBy similarity1
Binding sitei112SubstrateBy similarity1
Binding sitei175SubstrateBy similarity1
Binding sitei193SubstrateBy similarity1
Active sitei198Proton acceptorBy similarity1
Binding sitei280SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 14NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
Short name:
G6PD
Gene namesi
Name:ZWF1
Ordered Locus Names:ECU08_1850
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000000819 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU08_1850.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003817521 – 434Glucose-6-phosphate 1-dehydrogenaseAdd BLAST434

Proteomic databases

PRIDEiQ8SR89.

Expressioni

Developmental stagei

Expressed in late sporogonial stages.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi284813.NP_597312.1.

Structurei

3D structure databases

ProteinModelPortaliQ8SR89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 141Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0563. Eukaryota.
COG0364. LUCA.
HOGENOMiHOG000149291.
InParanoidiQ8SR89.
KOiK00036.
OMAiEFHETEM.
OrthoDBiEOG092C2MOH.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8SR89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVVIFGSSG DLAKRKLFPA LSRIDLEGVG VVGYARTKYN IEFSEVLQEV
60 70 80 90 100
GNYSPEFLSK VTYIPGPYDD LSKLKEVSDS ETVLYFSVPS SVYTCLFREI
110 120 130 140 150
SKLDYKVIGV EKPYGDSIES FMEIKGFDLG KTRFIDHYLL KPLVVAMPGI
160 170 180 190 200
IRETGAIREV MSNRYVKSVE IVSKEVLGGE GRHYFDKNGI IRDMVLSHMG
210 220 230 240 250
ELLGVVASDV TKPSRIMEAL ARMEVFKACT VDTERCIYGQ YDDYIKEIHK
260 270 280 290 300
DSSTETFCMV PISIGTPRWS KVPFIIVAGK GMNEKRTEII LEFRRDVFAK
310 320 330 340 350
CIELFSMPRQ SSCRIVHTNE IETVRLVFNI YPECEVFLEV LVGGEPVRYV
360 370 380 390 400
LHDKKEIDGL MHDSYGGYHD YEIIFDSLVR GKDFSSVSSQ EAELLWKVFN
410 420 430
PILSISKEDM LFYYSKGIDM PKEAEEMIRE IKDH
Length:434
Mass (Da):49,544
Last modified:June 1, 2002 - v1
Checksum:i0FD1BE2052CF0D45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590448 Genomic DNA. Translation: CAD26488.1.
RefSeqiNP_597312.1. NM_001041921.1.

Genome annotation databases

EnsemblFungiiCAD26488; CAD26488; CAD26488.
GeneIDi859734.
KEGGiecu:ECU08_1850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590448 Genomic DNA. Translation: CAD26488.1.
RefSeqiNP_597312.1. NM_001041921.1.

3D structure databases

ProteinModelPortaliQ8SR89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284813.NP_597312.1.

Proteomic databases

PRIDEiQ8SR89.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAD26488; CAD26488; CAD26488.
GeneIDi859734.
KEGGiecu:ECU08_1850.

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU08_1850.

Phylogenomic databases

eggNOGiKOG0563. Eukaryota.
COG0364. LUCA.
HOGENOMiHOG000149291.
InParanoidiQ8SR89.
KOiK00036.
OMAiEFHETEM.
OrthoDBiEOG092C2MOH.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG6PD_ENCCU
AccessioniPrimary (citable) accession number: Q8SR89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 1, 2002
Last modified: October 5, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.