Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8SR56 (VPS34_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable phosphatidylinositol 3-kinase VPS34 homolog

Short name=PI3-kinase VPS34
Short name=PI3K VPS34
Short name=PtdIns-3-kinase VPS34
EC=2.7.1.137
Gene names
Name:VPS34
Ordered Locus Names:ECU10_0590
OrganismEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]
Taxonomic identifier284813 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Phosphatidylinositol 3-kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II By similarity.

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

Subunit structure

Component of the autophagy-specific VPS34 PI3-kinase complex I composed; and of the VPS34 PI3-kinase complex II By similarity.

Subcellular location

Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein By similarity. Endosome membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Contains 1 PI3K/PI4K domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Probable phosphatidylinositol 3-kinase VPS34 homolog
PRO_0000388438

Regions

Domain202 – 444243PI3K/PI4K

Sequences

Sequence LengthMass (Da)Tools
Q8SR56 [UniParc].

Last modified May 29, 2013. Version 2.
Checksum: 1750449946A5C2F8

FASTA44652,007
        10         20         30         40         50         60 
MEEWDGDVFT WNAGKEDIYT LIESILRLLK NGCKGMENMV VKYLGTGATD SLYMYFPWLR 

        70         80         90        100        110        120 
GRDLESRLSV EHQIDRFWKI RFELHEERTN SMDRYEDIVS SFLALDTKCG ILGKLEGQMA 

       130        140        150        160        170        180 
LVDEIRRIYS ITSQGSPRKK RLREYRLHGT LCLHPGAGSM RLYASLFSLD ATVKEIIFPE 

       190        200        210        220        230        240 
IEIFPSSTFP ILVPLRTDRG VSRIIYKKGD DLTRDLFVLE TIRYMSRLMG VDLVTYKVIP 

       250        260        270        280        290        300 
LSRKEGIVEV VDGIDFTRIR SRKDLEMYIE EDRDPRHQES FEKRKVFVST LCGYSVACYV 

       310        320        330        340        350        360 
MGIGDRNPGN MMVTRDGKFF HIDFSHVFGR DPKPISSRIT IARPIRDYLV NDELIYQDFL 

       370        380        390        400        410        420 
ARSGEAFLQI RRSCRKIFVL WCILAQNRIF QFDLNEIIPF AQARLRMEMS EQRALELFEK 

       430        440 
EIRGAVGSLK TSVAHLINRV GMFLRR 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi."
Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P.
Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.
[2]"Identification of transcriptional signals in Encephalitozoon cuniculi widespread among Microsporidia phylum: support for accurate structural genome annotation."
Peyretaillade E., Goncalves O., Terrat S., Dugat-Bony E., Wincker P., Cornman R.S., Evans J.D., Delbac F., Peyret P.
BMC Genomics 10:607-607(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GB-M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590449 Genomic DNA. Translation: CAD25778.2.
RefSeqNP_586174.1. NM_001042007.1.

3D structure databases

ProteinModelPortalQ8SR56.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6035.ECU10_0590.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID859823.
KEGGecu:ECU10_0590.

Organism-specific databases

EuPathDBMicrosporidiaDB:ECU10_0590.

Phylogenomic databases

eggNOGCOG5032.
HOGENOMHOG000149280.
OrthoDBEOG7H79BZ.

Family and domain databases

Gene3D1.10.1070.11. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR015433. PI_Kinase.
[Graphical view]
PANTHERPTHR10048. PTHR10048. 1 hit.
PfamPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
SMARTSM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVPS34_ENCCU
AccessionPrimary (citable) accession number: Q8SR56
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 29, 2013
Last modified: April 16, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families