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Q8SR56 (VPS34_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable phosphatidylinositol 3-kinase VPS34 homolog
Short name=PI3-kinase VPS34
Short name=PI3K VPS34
Short name=PtdIns-3-kinase VPS34
EC=2.7.1.137
Gene names
Name:VPS34
Ordered Locus Names:ECU10_0590
OrganismEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]
Taxonomic identifier284813 [NCBI]
Taxonomic lineageEukaryota › Fungi › Microsporidia › Unikaryonidae › Encephalitozoon ›

Protein attributes

Sequence length458 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Phosphatidylinositol 3-kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II By similarity.

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

Subunit structure

Component of the autophagy-specific VPS34 PI3-kinase complex I composed; and of the VPS34 PI3-kinase complex II By similarity.

Subcellular location

Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein By similarity. Endosome membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Contains 1 PI3K/PI4K domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 458458Probable phosphatidylinositol 3-kinase VPS34 homolog
PRO_0000388438

Sequences

Sequence LengthMass (Da)Tools
Q8SR56 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 0B95F23A4F879D4D

FASTA45853,688
        10         20         30         40         50         60 
MWRYQNTRKY RPMEEWDGDV FTWNAGKEDI YTLIESILRL LKNGCKGMEN MVVKYLGTGA 

        70         80         90        100        110        120 
TDSLYMYFPW LRGRDLESRL SVEHQIDRFW KIRFELHEER TNSMDRYEDI VSSFLALDTK 

       130        140        150        160        170        180 
CGILGKLEGQ MALVDEIRRI YSITSQGSPR KKRLREYRLH GTLCLHPGAG SMRLYASLFS 

       190        200        210        220        230        240 
LDATVKEIIF PEIEIFPSST FPILVPLRTD RGVSRIIYKK GDDLTRDLFV LETIRYMSRL 

       250        260        270        280        290        300 
MGVDLVTYKV IPLSRKEGIV EVVDGIDFTR IRSRKDLEMY IEEDRDPRHQ ESFEKRKVFV 

       310        320        330        340        350        360 
STLCGYSVAC YVMGIGDRNP GNMMVTRDGK FFHIDFSHVF GRDPKPISSR ITIARPIRDY 

       370        380        390        400        410        420 
LVNDELIYQD FLARSGEAFL QIRRSCRKIF VLWCILAQNR IFQFDLNEII PFAQARLRME 

       430        440        450 
MSEQRALELF EKEIRGAVGS LKTSVAHLIN RVGMFLRR

« Hide

References

[1]"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi."
Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P.
Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL590449 Genomic DNA. Translation: CAD25778.1.
RefSeqNP_586174.1. NM_001042007.1.

3D structure databases

HSSPHSSP built from PDB template 1E8Y based on UniProtKB P48736.
ProteinModelPortalQ8SR56.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID859823.
KEGGecu:ECU10_0590.

Phylogenomic databases

eggNOGCOG5032.
HOGENOMHOG000149280.

Family and domain databases

Gene3D1.10.1070.11. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR015433. PI_Kinase.
[Graphical view]
PANTHERPTHR10048. PTHR10048. 1 hit.
PfamPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
SMARTSM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00915. PI3_4_KINASE_1. False negative.
PS00916. PI3_4_KINASE_2. False negative.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVPS34_ENCCU
AccessionPrimary (citable) accession number: Q8SR56
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: June 1, 2002
Last modified: May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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