Q8SR40 (GPDH_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glycerol-3-phosphate dehydrogenase
Short name=GPDH
EC=1.1.5.3

Gene names

Ordered Locus Names:

ECU10_0870

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.
Cofactor	FAD By similarity.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.
Subcellular location	Cytoplasm Ref.2.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycerol catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	Probable glycerol-3-phosphate dehydrogenase		PRO_0000383045
Regions
	Nucleotide binding	57 – 85	29	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8SR40 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 20942D4D6F7AC40E	FASTA	614	68,640
	10 20 30 40 50 60 MLVALVVLFL SVFMAMKFLY KRIFVASRLK MIEKPSEDWE PASREAMIER LRSEVFDLVV 70 80 90 100 110 120 VGGGSTGAGC ALDGATRGLK VALVDAGDFG SGTSSKSTKL VHGGVRYLAK AVSNLDWSQY 130 140 150 160 170 180 KLVWQALGER TTMFEISPYL TNSIKIMVPI YSKILIPYYY VGLKLYDWIS GFKSLGKSYF 190 200 210 220 230 240 IDRKEAVDAF PHINKKNLCG AMVYFDGQQD DARNNVMIVM TAVCHGAVAA NHVSARSLMI 250 260 270 280 290 300 EGGKIVGVRC RDEITGSEIE IRGTGVINST GNLADDLRRM DDADAREIIV QSSGTHIVIP 310 320 330 340 350 360 KEYAPKEMGF LDPLTSDNRI AFFMPWMGKT IVGSTDIKTK TELSPSPTEE DLEFLIHEVQ 370 380 390 400 410 420 AYTSMHPKLT RDEVSAVWTG IRPLVKDPDV SDTGSIVRKH FVRIEKNGLL TVTGGKWTIY 430 440 450 460 470 480 RKMAEDAIDL AISAFSLKPS GPCVTKYVRI LGGDGYTKNT WASIQKELGV PKNVAERLAR 490 500 510 520 530 540 SYGTRALRLS SYIKKNRKKV LSVKYSYLIE EVEYCIDNEM AVKVCDVLCN RLMIGLMDVK 550 560 570 580 590 600 EAYQCIDKVL GVFKKKHGWD ADRCNREEAD AIRMLDKYGL QILRGCGQDA SSLQMECPEE 610 KRHRGERRLP PQEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[2]	"Distinct localization patterns of two putative mitochondrial proteins in the microsporidian Encephalitozoon cuniculi." Williams B.A.P., Cali A., Takvorian P.M., Keeling P.J. J. Eukaryot. Microbiol. 55:131-133(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590449 Genomic DNA. Translation: CAD25806.1.
RefSeq	NP_586202.1. NM_001042035.1.
3D structure databases
ProteinModelPortal	Q8SR40.
ModBase	Search...
Protein-protein interaction databases
STRING	6035.ECU10_0870.
Proteomic databases
PRIDE	Q8SR40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	859851.
KEGG	ecu:ECU10_0870.
Phylogenomic databases
eggNOG	COG0578.
HOGENOM	HOG000004813.
KO	K00111.
OMA	EWMLLPI.
Enzyme and pathway databases
UniPathway	UPA00618; UER00673.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR000447. G3P_DH_FAD-dep. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
PROSITE	PS00977. FAD_G3PDH_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPDH_ENCCU

Accession

Primary (citable) accession number: Q8SR40

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program